Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,324,952 A→G 5.0% intergenic (‑41/+49) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,324,9520AG 5.0% 1064.1 / 30.8 321intergenic (‑41/+49)folP/ftsH7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
Reads supporting (aligned to +/- strand):  ref base A (159/143);  new base G (6/10);  total (166/155)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-01

Only 100 of 325 total aligned reads displayed.

GCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAG  >  NC_000913/3324803‑3325098
                                                                                                                                                     |                                                                                                                                                  
  gAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGc                                                                                                                                                  >  1:3763075/1‑150 (MQ=255)
        tGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCCTTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGccc                                                                                                                                            >  2:4771421/1‑150 (MQ=255)
        tGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTGTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGccc                                                                                                                                            >  2:4312233/1‑150 (MQ=255)
           gTGCCACCATCCTAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTACGGTCCAGTGAAGTACCCTGGGAAAAGTGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgg                                                                                                                                         <  1:11221/150‑1 (MQ=255)
           gTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgg                                                                                                                                         >  2:3949074/1‑150 (MQ=255)
            tGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCTGTGATGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCggg                                                                                                                                        <  1:271798/150‑1 (MQ=255)
            tGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGATAAGGTCCAGTGAAGTACCCTGGGCAAATAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCggg                                                                                                                                        <  1:5352938/150‑1 (MQ=255)
              ccaccaTCCGAAAAGGAATCCGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAATATTTTCATTATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGt                                                                                                                                      <  1:5224561/150‑1 (MQ=255)
              ccaccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGTGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGt                                                                                                                                      <  2:1913140/150‑1 (MQ=255)
               caccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGtt                                                                                                                                     <  1:4256028/150‑1 (MQ=255)
                accaTCCGAAAAGTAATCTGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGTTGTTATCCCTGGTATGATTTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttt                                                                                                                                    <  1:4074350/150‑1 (MQ=255)
                accaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGttt                                                                                                                                    >  1:1014671/1‑150 (MQ=255)
                   aTCCTAAATGGAATCCGGCGTGACGTTGAGGATCCCCATTACGTTAGTATGGCTATGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATTATGTTTTCCCTGTTTTGTATTTATAATAAAAAACCCCGGCGCACGCCCCGGGGTTTTCg                                                                                                                                 <  1:77660/150‑1 (MQ=255)
                   aTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCg                                                                                                                                 <  1:2837005/150‑1 (MQ=255)
                    tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCCTTACGTGAGGAGGGCGAAGGGCCAGTGAAGTACCCGGGGCAAAGAGGTGCATGATGTTATCCCTGGTATGAATGGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCgg                                                                                                                                <  2:3163009/150‑1 (MQ=255)
                    tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttc                                                                                                                                <  2:665903/150‑4 (MQ=255)
                       gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                             >  2:3617335/1‑150 (MQ=255)
                       gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                             >  1:4411034/1‑150 (MQ=255)
                         aaaGGAATCAGGCGTGACGTTGAGGATCCCGATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACaa                                                                                                                           <  2:5061838/150‑1 (MQ=255)
                         aaaGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACaa                                                                                                                           <  2:1874500/150‑1 (MQ=255)
                           aGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAAt                                                                                                                         <  2:791130/150‑1 (MQ=255)
                             gactCAGGCGTGACGTTGAGGATCCCCAGTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAAGTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTCCAAATAc                                                                                                                       >  1:1764357/4‑150 (MQ=255)
                             gAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATAc                                                                                                                       <  1:3060433/150‑1 (MQ=255)
                              aaTCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACa                                                                                                                      <  2:4471572/150‑1 (MQ=255)
                                  aGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCa                                                                                                                  <  1:4895985/150‑1 (MQ=255)
                                    gCGTGTCGTTGTGGATCCCCATTCCTTGAGGATGGCTATGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTTATATGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATc                                                                                                                <  1:4559693/150‑1 (MQ=255)
                                    gCGTGACGTTGATGATCCCCATGCCGTGAGGAGGGCGAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGAAGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATc                                                                                                                <  2:5295099/150‑1 (MQ=255)
                                      gTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTg                                                                                                              >  1:2587662/1‑150 (MQ=255)
                                       tgccGTTGAGGAGCCCCATTACGTGAGGAGGGCTACGGTCCCGTGCAGTACCCTGGGCAAAGAGTTTCATGATGTTCTCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGa                                                                                                             <  2:3904138/147‑1 (MQ=255)
                                          cGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGc                                                                                                          <  2:1336093/150‑1 (MQ=255)
                                          cGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGc                                                                                                          >  2:3806072/1‑150 (MQ=255)
                                           gTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGTTGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCg                                                                                                         <  1:3467052/150‑1 (MQ=255)
                                           gTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCGCGGGGTTTTCGGTACATATACAGTCATCTGATGCg                                                                                                         <  2:567522/150‑1 (MQ=255)
                                           gTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCg                                                                                                         >  1:4727121/1‑150 (MQ=255)
                                            ttGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCgg                                                                                                        <  1:3899739/150‑1 (MQ=255)
                                               aGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGaa                                                                                                     <  1:4214626/150‑1 (MQ=255)
                                               aGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGaa                                                                                                     <  1:5083594/150‑1 (MQ=255)
                                                ggATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                    >  2:2102664/1‑150 (MQ=255)
                                                 gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAact                                                                                                   >  2:3035599/1‑150 (MQ=255)
                                                 gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAact                                                                                                   >  1:3394767/1‑150 (MQ=255)
                                                     cccATTACGTGAGGATGGCTACGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATACGAAAAAACCCCGGAGCGCGCCCCGGGGTTTTCGGTACCCAGACAGTCCTCTGATGCGGGAacttacg                                                                                               >  2:2660887/1‑149 (MQ=255)
                                                       cATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTg                                                                                             >  1:2082783/1‑150 (MQ=255)
                                                          taagTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTATCAAGATGTTATCCCTGGTATGAATTGATAAGAAAAAACACCCGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCg                                                                                          >  2:1219295/4‑150 (MQ=255)
                                                          tACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCg                                                                                          <  1:2026005/150‑1 (MQ=255)
                                                           aCGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGc                                                                                         >  2:513912/1‑150 (MQ=255)
                                                           aCGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGc                                                                                         >  1:2786236/1‑150 (MQ=255)
                                                                  gATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTg                                                                                  >  1:2213145/1‑150 (MQ=255)
                                                                      gCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGctct                                                                              <  1:5284502/150‑1 (MQ=255)
                                                                       cTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                             >  1:3538332/1‑150 (MQ=255)
                                                                           ggTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACAt                                                                         >  2:5472001/1‑150 (MQ=255)
                                                                             tCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATgg                                                                       >  2:4567911/1‑150 (MQ=255)
                                                                             tCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATgg                                                                       <  1:2492449/150‑1 (MQ=255)
                                                                               cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtg                                                                     <  1:1584961/150‑1 (MQ=255)
                                                                                  tGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTa                                                                  >  2:2946233/1‑150 (MQ=255)
                                                                                    aaGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAcc                                                                >  2:5341100/1‑150 (MQ=255)
                                                                                     aGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAccc                                                               <  2:2740733/150‑1 (MQ=255)
                                                                                         cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGt                                                           <  1:2371677/150‑1 (MQ=255)
                                                                                          ccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGtt                                                          >  1:3737475/1‑150 (MQ=255)
                                                                                               gCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCg                                                     >  2:1052743/1‑150 (MQ=255)
                                                                                                cAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTCCCCGGGTTCGGCGt                                                    >  2:1624189/1‑150 (MQ=255)
                                                                                                 aaaGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTa                                                   >  1:921936/1‑150 (MQ=255)
                                                                                                 aaaGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTa                                                   >  1:3641613/1‑150 (MQ=255)
                                                                                                   agagTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTAcg                                                 <  2:4132118/150‑1 (MQ=255)
                                                                                                       tttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                             >  2:3270168/1‑150 (MQ=255)
                                                                                                       tttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                             >  2:2762496/1‑150 (MQ=255)
                                                                                                          cATGATGTTATCCCTGGTATGACTTGATAAGAACAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtca                                          <  1:1364865/150‑1 (MQ=255)
                                                                                                             gatgTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtcatca                                       <  1:62277/150‑1 (MQ=255)
                                                                                                               tgTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAAc                                     <  2:3683115/150‑1 (MQ=255)
                                                                                                                   aTCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGa                                 >  1:2560905/1‑150 (MQ=255)
                                                                                                                   aTCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGa                                 <  2:1498475/150‑1 (MQ=255)
                                                                                                                    tCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGAc                                >  2:1543001/1‑150 (MQ=255)
                                                                                                                      ccTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACga                              >  1:1210534/1‑150 (MQ=255)
                                                                                                                        tGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTCCCCGGGTTCGGCGTACGCGGTTCATCAACCGGACgagg                            >  2:3667289/1‑150 (MQ=255)
                                                                                                                        tGGTATGAAGTGCTAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATACAGTCCTCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTGACCCGGGTTCGGCGGACGCGGTTCATCAACCGGACgagg                            >  2:4890133/1‑150 (MQ=255)
                                                                                                                             tGAATTGATAAGAAAAAACCCCGGCGCACTCCCCGGGGATTTCTGTAAAAATACAGTCATCTGATGCGGGAACATACTAGTCGCCTAACTGATCTGACATCGTGTTCACCGGGTTCGGCGTACGCGCTTCATCAACCGGACGAGGAGCCt                       >  2:470432/1‑150 (MQ=255)
                                                                                                                              gAAGTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCtt                      <  2:231361/150‑1 (MQ=255)
                                                                                                                               aaatGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCttt                     <  1:4917295/147‑1 (MQ=255)
                                                                                                                               aaTTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCttt                     <  1:4789762/150‑1 (MQ=255)
                                                                                                                                aTTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                    >  1:1829245/1‑150 (MQ=255)
                                                                                                                                 ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTgg                   <  1:2319433/150‑1 (MQ=255)
                                                                                                                                  tGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGa                  >  1:3670594/1‑150 (MQ=255)
                                                                                                                                  tGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGGACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGa                  <  1:4346926/150‑1 (MQ=255)
                                                                                                                                   gATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGTGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCTGACGAGGAGCCTTTGGAc                 <  1:3283037/150‑1 (MQ=255)
                                                                                                                                   gATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGAc                 <  1:3780593/150‑1 (MQ=255)
                                                                                                                                    aTAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACt                <  2:2344229/150‑1 (MQ=255)
                                                                                                                                     tAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAGCTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTa               >  1:4841215/1‑150 (MQ=255)
                                                                                                                                     tAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTa               >  2:1856256/1‑150 (MQ=255)
                                                                                                                                      aaGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAc              >  1:5148478/1‑150 (MQ=255)
                                                                                                                                       aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAcc             >  1:1037503/1‑150 (MQ=255)
                                                                                                                                        ccgaaaaCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCa            >  2:2476601/4‑150 (MQ=255)
                                                                                                                                         aaaaaaCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCAt           >  1:580801/1‑150 (MQ=255)
                                                                                                                                          aaaaaCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCAtt          <  2:4943885/150‑1 (MQ=255)
                                                                                                                                             aaCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc       <  2:2564902/150‑1 (MQ=255)
                                                                                                                                             aaCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc       >  1:707438/1‑150 (MQ=255)
                                                                                                                                               ccccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc     <  1:4025447/150‑1 (MQ=255)
                                                                                                                                                cccGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc    >  2:3358866/1‑150 (MQ=255)
                                                                                                                                                cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc    >  1:5036206/1‑150 (MQ=255)
                                                                                                                                                cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc    >  2:182674/1‑150 (MQ=255)
                                                                                                                                                cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGGTACCCGGGTTCGGCGTCCGCGGTTCAGCAACCGGACGAGGAGCCGTGGGACTACCCGGGTCGcc    >  2:3421785/1‑150 (MQ=255)
                                                                                                                                                  cGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAg  >  1:595948/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCGAGTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAG  >  NC_000913/3324803‑3325098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: