Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,038 T→A 13.4% intergenic (+21/‑177) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0380TA13.4% 589.4 / 10.7 219intergenic (+21/‑177)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (16/154);  new base A (22/2);  total (62/157)
Fisher's exact test for biased strand distribution p-value = 9.19e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

TGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTG  >  NC_000913/3780890‑3781185
                                                                                                                                                    |                                                                                                                                                     
tGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTa                                                                                                                                                      >  1:3221427/1‑150 (MQ=255)
 gAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTaa                                                                                                                                                     <  1:1175953/150‑1 (MQ=255)
  aaaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAc                                                                                                                                                    <  2:1785670/150‑1 (MQ=255)
   aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAcc                                                                                                                                                   <  1:4924095/150‑1 (MQ=255)
   aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAcc                                                                                                                                                   <  1:2196269/150‑1 (MQ=255)
     gTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCct                                                                                                                                                 <  1:3291638/150‑1 (MQ=255)
        gCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCtctc                                                                                                                                              <  2:5091661/150‑1 (MQ=255)
          gATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCCAAaccacaacc                                                                                                                                            >  2:4811438/1‑144 (MQ=255)
            tGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTc                                                                                                                                          <  1:4993895/150‑1 (MQ=255)
             gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAATGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCGCACCCACAcccccc                                                                                                                                         >  2:2792068/1‑147 (MQ=255)
             gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCa                                                                                                                                         <  2:5357051/150‑1 (MQ=255)
             gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCa                                                                                                                                         <  2:3894859/150‑1 (MQ=255)
              aCGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                                       <  1:3883238/150‑1 (MQ=255)
               cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAgg                                                                                                                                      <  1:2976930/150‑1 (MQ=255)
                 cTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGGTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGGGTGCCCCCCCCCCCCCCCACCCACTcccccccggc                                                                                                                                     >  2:3483348/1‑143 (MQ=255)
                   gACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGgaga                                                                                                                                  <  1:553606/150‑1 (MQ=255)
                   gACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACCCCCGAACCCACACCCACAGGgagt                                                                                                                                  >  1:4504247/1‑149 (MQ=255)
                   gACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACCCCCCAAACCACAACCCCAgggggt                                                                                                                                  >  2:4230222/1‑147 (MQ=255)
                    aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                                                 <  1:1920774/150‑1 (MQ=255)
                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                <  1:2498364/150‑1 (MQ=255)
                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                <  2:2254349/150‑1 (MQ=255)
                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                                                <  1:4055032/150‑1 (MQ=255)
                      tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                                               <  1:5402464/150‑1 (MQ=255)
                        gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                                             <  1:1646695/150‑1 (MQ=255)
                        gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                                             <  1:3371994/150‑1 (MQ=255)
                        gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCCCCCAAAAACACACCCACACGGAGAggggg                                                                                                                             >  1:2065403/1‑149 (MQ=255)
                         cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGAc                                                                                                                            <  2:1252504/150‑1 (MQ=255)
                         cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCAAACCCACACCCGCAgggggggggggc                                                                                                                            >  2:5283223/1‑148 (MQ=255)
                          gcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggttag                                                                                                                           <  2:794925/150‑5 (MQ=255)
                          gagAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggttag                                                                                                                           <  2:3221427/148‑5 (MQ=255)
                           cgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCAAAACCACACCCAACGGGCGGGGGGAccc                                                                                                                          >  1:1048209/1‑149 (MQ=255)
                           cgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCAAAACCCCACCCACACGGCGAGGGGACCg                                                                                                                          >  1:4411104/1‑150 (MQ=255)
                             aaaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                                                                        <  2:3061837/150‑1 (MQ=255)
                               aTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                                      <  2:1192791/150‑1 (MQ=255)
                                tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGTGTTGCCTGCGGCACTGGCTCCCATGGCTAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCgg                                                                                                                     <  1:106790/150‑1 (MQ=255)
                                tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCgg                                                                                                                     <  1:1378163/150‑1 (MQ=255)
                                   aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCAAACCCAAACCCACTACCTCAGGGAGAGGGGACCGTTCGgcgc                                                                                                                  >  2:1379039/1‑150 (MQ=255)
                                      aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCCCCCCCCAAACCCCAACCCCAGGGGGGGGGGGCCCGTCGgcgccgg                                                                                                               >  2:1168956/1‑147 (MQ=255)
                                      aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTCGCCCCCACACCCAAAACCACAACCACAGGGAGAGGGGACCCTTCGGCGCagt                                                                                                               >  2:3467654/1‑150 (MQ=255)
                                      aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCGAACCCACACCCGCAGGGAGAGGGGCCCCTTCGGCGCggt                                                                                                               >  2:4063004/1‑150 (MQ=255)
                                       gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACTCCCACAGGGAGAGGGGGCCGTTCGGCGCtgta                                                                                                              >  1:1837414/1‑150 (MQ=255)
                                        cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtat                                                                                                             <  2:411534/150‑1 (MQ=255)
                                           aaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatgta                                                                                                          <  2:3785217/150‑1 (MQ=255)
                                              tACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTc                                                                                                       <  1:5011218/150‑1 (MQ=255)
                                                cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACACACACCCACACGGGGGGGGGGCCCGTCGGCGCGTTATGTAcaacc                                                                                                     >  1:3192942/1‑146 (MQ=255)
                                                 gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAg                                                                                                                                       <  2:4370468/115‑1 (MQ=255)
                                                 gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                                                                    <  1:5176908/150‑1 (MQ=255)
                                                   aaGATTCGATGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGTCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCAACCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTTCTCCCTCa                                                                                                  <  2:459222/150‑1 (MQ=255)
                                                    aGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCTCCCCCTAACCCTAACCCTCAGGGGGAGGGGACCCTTCGGCGCTGTATGTTCTCCCTCAc                                                                                                 >  1:3048118/1‑150 (MQ=255)
                                                     gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCAct                                                                                                <  1:1778339/150‑1 (MQ=255)
                                                       ttCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCTCGCCCGCAGGGAGAGGGGACCCTTCGGCGCTGTATGTAATCCCACAcact                                                                                              >  2:3661031/1‑150 (MQ=255)
                                                         cGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                            <  2:5383375/150‑1 (MQ=255)
                                                                gCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGaca                                                                                     <  2:1282730/150‑1 (MQ=255)
                                                                 cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGacac                                                                                    <  2:4668808/150‑1 (MQ=255)
                                                                    gggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCAAAACCAAAACCAAAACCAAAGGGAGAGGGGAACCTTAGGCGCAGTATGTACACCCACACACTGAAACGACAcccc                                                                                 >  2:415574/1‑148 (MQ=255)
                                                                     gggtGGGTAATGTGTTGCCTGCGGCACTGGCTCCCTTGGCTATTGGGTTTGCGGCATTGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGTGTGGGGACCGTTCGGCGGTTTTTTTACTCCCTCACTCTGAAACGACACCGCa                                                                                <  1:3748319/147‑1 (MQ=255)
                                                                     ggCTGGGTAATGAGTTGCCTGCGGCACTGGCTCCCATGGCGACAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAAACCGCTACCTCCAGGGGTGGGGCCCGTTCGGCGCAGTATGTACTACCGCCCACAGCAACGACACCCCa                                                                                >  1:5355804/1‑150 (MQ=255)
                                                                        tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCGAAACCCAAACCACAGGGAGAGGGGACCCCTAGGCGCGGTATTTAAACACACACACAGAAACCACAccccccac                                                                             >  2:3941882/1‑144 (MQ=255)
                                                                          ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtt                                                                           <  2:297046/150‑1 (MQ=255)
                                                                          ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCCCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtt                                                                           <  2:2578880/150‑1 (MQ=255)
                                                                            tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                         <  2:4462518/150‑1 (MQ=255)
                                                                             aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                        <  1:1518058/150‑1 (MQ=255)
                                                                              aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAAACCCCACCCACACGGGGAGGGGGCCCGGCGGCGCGGGTTGTTGAACCACACGCTGGAAAGACACCCCCCACtttttt                                                                       >  1:394599/1‑150 (MQ=255)
                                                                               agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                      <  1:143419/150‑1 (MQ=255)
                                                                               agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                      <  1:857508/150‑1 (MQ=255)
                                                                               agagTTGCCTGCGGCACTGGCGCCCAGGGCGACAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTAGGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                      <  1:2202964/150‑1 (MQ=255)
                                                                                  gTTGCCTGCGGCAGTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                   <  1:4160315/150‑1 (MQ=255)
                                                                                  gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                   <  1:4811452/150‑1 (MQ=255)
                                                                                  gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                   <  1:765853/150‑1 (MQ=255)
                                                                                     gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCt                                                                <  1:2396102/150‑1 (MQ=255)
                                                                                      ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                               <  2:2761367/150‑1 (MQ=255)
                                                                                       cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                              <  1:3548565/150‑1 (MQ=255)
                                                                                            gCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCt                                                        <  1:4665806/150‑1 (MQ=255)
                                                                                                tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                    <  2:4852780/150‑1 (MQ=255)
                                                                                                 ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGACACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                   <  2:2347481/150‑1 (MQ=255)
                                                                                                 ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                   <  1:421941/150‑1 (MQ=255)
                                                                                                    tCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACCCCCAAACCCAAGACCGCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACAACCACACAAAGAAACGACACCCCACACATTTTTTAACCCACGCCCCACCCGGgag                                                >  2:5000626/1‑150 (MQ=255)
                                                                                                     cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                               <  1:2433239/150‑1 (MQ=255)
                                                                                                      ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                              <  2:5381310/150‑1 (MQ=255)
                                                                                                       cagggCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                             <  1:1884528/147‑1 (MQ=255)
                                                                                                       cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                             <  1:2580567/150‑1 (MQ=255)
                                                                                                        aTGGCGAAAGGGAATGCGGCATAGTCATTTGCGCCCCCTCACCCAAACCCTCTCCCTCAGGGTGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                            <  1:4148204/150‑1 (MQ=255)
                                                                                                           gCGAAAGGGAATGCGGCATCGTCGTTTGCGCCCCCGCCCCCTCACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAACCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                         <  2:3212107/150‑1 (MQ=255)
                                                                                                           gCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                         <  1:1165315/150‑1 (MQ=255)
                                                                                                             gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgggagaaa                                       <  2:576631/150‑6 (MQ=255)
                                                                                                              aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCCAAACCACAACCAAACGGGGGGGGGGCCCGTACGCGCGGTTTGTTCGCCCACACACAGAAACGAAACCCCACAAATTTTTTAGACCACCCCCCCCCCGGGGGAGGGGCgggg                                      >  2:2147661/1‑150 (MQ=255)
                                                                                                               aaGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCGAAACCACTCCCGCAGGGAGAGGGGGCCCGTGGGCGCTGTGTGTAATCCCGCACGATGAAACGACACCGCCCACTTTTTTTTTACCCCGCCCCGCCCGGGGGGCGGGCGgggg                                      >  2:4901423/1‑150 (MQ=255)
                                                                                                                  ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCACACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                  <  1:2775131/150‑1 (MQ=255)
                                                                                                                  ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                  <  2:2915797/150‑1 (MQ=255)
                                                                                                                  ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                  <  1:3630399/150‑1 (MQ=255)
                                                                                                                   gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                 <  2:4691986/150‑1 (MQ=255)
                                                                                                                    aaTGCGGCATCGTCGTTTGCGCCCCCTCACCCTCCCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                <  2:3069699/150‑1 (MQ=255)
                                                                                                                       gCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaa                             <  2:1977163/150‑1 (MQ=255)
                                                                                                                        cGGCATAGTCGTTTGCGCCCCCTCACCCTCACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaa                            <  2:2399066/150‑1 (MQ=255)
                                                                                                                                          ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCa          >  2:4780783/1‑150 (MQ=255)
                                                                                                                                             cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt       >  1:3304708/1‑150 (MQ=255)
                                                                                                                                                aCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTc    >  1:3130488/1‑150 (MQ=255)
                                                                                                                                                 cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt   >  1:5223571/1‑150 (MQ=255)
                                                                                                                                                  ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg  >  1:1095656/1‑150 (MQ=255)
                                                                                                                                                  ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg  >  1:4322308/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTG  >  NC_000913/3780890‑3781185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: