Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,131 T→G 5.2% intergenic (+114/‑84) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1310TG 5.2% 689.6 / 15.1 204intergenic (+114/‑84)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (146/42);  new base G (4/6);  total (155/49)
Fisher's exact test for biased strand distribution p-value = 1.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

GCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGC  >  NC_000913/3780982‑3781280
                                                                                                                                                       |                                                                                                                                                       
    tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                                                                     <  2:4852780/150‑1 (MQ=255)
    gggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                                                                     <  2:2967301/149‑1 (MQ=255)
     ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                                                    <  1:807051/150‑1 (MQ=255)
     ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                                                    <  1:421941/150‑1 (MQ=255)
     ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                                                    <  1:203431/150‑1 (MQ=255)
        tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCAACCGAAACCACTCCCGCAGGGGGGGGGGACCCTTCGGCGCGGGATGTTATACCGCACAAGGGAACGACACCCCACGCTTTTTTTATGCCGCGCCCCGCCCGGgag                                                                                                                                                 >  2:3678477/1‑150 (MQ=255)
        tCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACCCCCAAACCCAAGACCGCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACAACCACACAAAGAAACGACACCCCACACATTTTTTAACCCACGCCCCACCCGGgag                                                                                                                                                 >  2:5000626/1‑150 (MQ=255)
          ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                                                               <  2:5381310/150‑1 (MQ=255)
           cagggCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                                                                                              <  1:1884528/147‑1 (MQ=255)
            aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCGCCCTCTCCCCGGAGGGGCGAggg                                                                                                                                             <  1:2185103/150‑1 (MQ=255)
             tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGc                                                                                                                                            <  2:5138428/150‑1 (MQ=255)
               gCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                                                                                                          <  1:1165315/150‑1 (MQ=255)
               gCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                                                                                                          <  1:3473952/150‑1 (MQ=255)
                  aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCCAAACCACAACCAAACGGGGGGGGGGCCCGTACGCGCGGTTTGTTCGCCCACACACAGAAACGAAACCCCACAAATTTTTTAGACCACCCCCCCCCCGGGGGAGGGGCgggg                                                                                                                                       >  2:2147661/1‑150 (MQ=255)
                   aaGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCGAAACCACTCCCGCAGGGAGAGGGGGCCCGTGGGCGCTGTGTGTAATCCCGCACGATGAAACGACACCGCCCACTTTTTTTTTACCCCGCCCCGCCCGGGGGGCGGGCGgggg                                                                                                                                       >  2:4901423/1‑150 (MQ=255)
                     gggACTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                                                                    <  2:1713856/150‑1 (MQ=255)
                      ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCACACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                                                   <  1:2775131/150‑1 (MQ=255)
                      ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                                                   <  1:3630399/150‑1 (MQ=255)
                       gactGCGGCATAGTCGTTTGCGCCCCCTCCCCCAAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACACTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                  <  1:1384085/147‑1 (MQ=255)
                       gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGTGGGGACCGTTCGGCGCTTTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                  <  1:2994911/150‑1 (MQ=255)
                       gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                  <  2:1263278/150‑1 (MQ=255)
                       gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                  <  2:4691986/150‑1 (MQ=255)
                        aaTGCGGCATCGTCGTTTGCGCCCCCTCACCCTCCCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                                                                                                 <  2:3069699/150‑1 (MQ=255)
                           gCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑aa                                                                                                                            <  2:1977163/150‑1 (MQ=255)
                            cGGCATAGTCGTTTGCGCCCCCTCACCCTCACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑aaa                                                                                                                           <  2:2399066/150‑1 (MQ=255)
                                                 cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAAtt                                                                                                      >  1:3304708/1‑150 (MQ=255)
                                                  tCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAAttt                                                                                                     >  2:2765964/1‑150 (MQ=255)
                                                    aCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTc                                                                                                   >  1:3130488/1‑150 (MQ=255)
                                                      ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                                                 >  1:4322308/1‑150 (MQ=255)
                                                      ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                                                 >  2:4803615/1‑150 (MQ=255)
                                                      ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                                                                 >  1:1095656/1‑150 (MQ=255)
                                                          aCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTAt                                                                                             >  2:1792200/1‑150 (MQ=255)
                                                           cccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                                                            >  1:4784924/1‑150 (MQ=255)
                                                             ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTTAATCGAAACCGCAGTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑TAAAGGCCGCACTGCTCTCCAATTTTCTGCTATcat                                                                                          >  2:3697461/1‑148 (MQ=255)
                                                              tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                                         >  2:4739790/1‑150 (MQ=255)
                                                              tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                                         >  2:4668073/1‑150 (MQ=255)
                                                                         ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                                               >  2:4927727/1‑150 (MQ=255)
                                                                         ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                                               >  1:3095205/1‑150 (MQ=255)
                                                                          gagaGTGGACCGTTCGGCGCTGTATGTACTCCCTCACACTGAAACGACACCGCACTCTTTTTTTCTACCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCACTTTTCTGCTATCCTGCCCCCGCACTa                                                                              >  2:1114245/1‑150 (MQ=255)
                                                                             agGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                           >  1:4966646/1‑150 (MQ=255)
                                                                             agGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                           >  1:3760995/1‑150 (MQ=255)
                                                                               gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                         >  1:2652742/1‑150 (MQ=255)
                                                                               gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                         >  1:2595318/1‑150 (MQ=255)
                                                                               gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                         >  1:5489831/1‑150 (MQ=255)
                                                                                      ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                                                  >  2:5411407/1‑150 (MQ=255)
                                                                                                tatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                                                        >  2:877711/1‑150 (MQ=255)
                                                                                                  tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                                                      >  2:4630745/1‑150 (MQ=255)
                                                                                                  tgtaCTCCCTCACTCTGAAACGACACCGCACACTTTTTTTCTCCCTCGCCCCTCCCGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCCCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                                                      >  2:1733885/1‑150 (MQ=255)
                                                                                                     acacacTCACTCTGAAACGACACCGCACTCTTTTTTTCTAACTCGCCCCACCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAACGCCGAACTGCTCAACAATTTTCTGCTATACTGCCACCGCACTAAGGCGGCAGTATGCTAAACATCGTACt                                                   >  2:3896559/6‑150 (MQ=255)
                                                                                                     aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCCCGAAGGGGGCAGTATGCTAAACATCGTACt                                                   >  2:3059075/1‑150 (MQ=255)
                                                                                                     aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt                                                   >  2:2322718/1‑150 (MQ=255)
                                                                                                     aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt                                                   >  1:4974264/1‑150 (MQ=255)
                                                                                                     aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt                                                   >  2:4058616/1‑150 (MQ=255)
                                                                                                     aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt                                                   >  1:4439467/1‑150 (MQ=255)
                                                                                                       tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                                                 >  2:3147834/1‑150 (MQ=255)
                                                                                                       tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                                                 >  1:2474836/1‑150 (MQ=255)
                                                                                                         ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTGTGCTCAACATCGTACTTTAc                                               >  1:4001834/1‑150 (MQ=255)
                                                                                                         ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                               >  2:1414433/1‑150 (MQ=255)
                                                                                                         ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                               >  2:2716487/1‑150 (MQ=255)
                                                                                                           tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                             >  2:1464396/1‑150 (MQ=255)
                                                                                                           tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                             >  2:3558639/1‑150 (MQ=255)
                                                                                                             aCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAc                                           >  1:4146134/1‑150 (MQ=255)
                                                                                                               tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                                         >  1:694867/1‑150 (MQ=255)
                                                                                                                ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                        >  1:4065711/1‑150 (MQ=255)
                                                                                                                ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                        >  2:4341263/1‑150 (MQ=255)
                                                                                                                ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                        >  1:227973/1‑150 (MQ=255)
                                                                                                                 tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa                                       >  2:3019659/1‑150 (MQ=255)
                                                                                                                  gAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaa                                      >  2:4399718/1‑150 (MQ=255)
                                                                                                                   aaaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa                                     >  2:690532/1‑150 (MQ=255)
                                                                                                                    aaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAt                                    >  1:529737/1‑150 (MQ=255)
                                                                                                                      cGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTc                                  >  1:124604/1‑150 (MQ=255)
                                                                                                                            cGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa                            >  2:3011248/1‑150 (MQ=255)
                                                                                                                              cttttttttttcCTCCCGCGCCCGGGGGGTGTGTGGGGGGGGGTGGGGGC‑‑ACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAAt                          <  2:1837414/145‑1 (MQ=255)
                                                                                                                              cACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAAt                          >  2:2583579/1‑150 (MQ=255)
                                                                                                                                 tctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                       >  2:3728723/1‑150 (MQ=255)
                                                                                                                                 tctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                       >  2:1060616/1‑150 (MQ=255)
                                                                                                                                   tttttttGTCCCCCCCCCCCCGGGGGGGTGGGGGGGGGGGGGGGGCCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                       <  2:5358390/150‑1 (MQ=255)
                                                                                                                                   tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     >  2:1618809/1‑150 (MQ=255)
                                                                                                                                   tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     >  1:3096369/1‑150 (MQ=255)
                                                                                                                                   tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTATCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     >  1:914468/1‑150 (MQ=255)
                                                                                                                                   ttttttgCCCCCCCGCCCGGGGGGGGTGGGGGGGGGGGGGTGGGC‑‑ACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     <  2:4527743/150‑1 (MQ=255)
                                                                                                                                    ttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGc                    >  1:4008886/1‑150 (MQ=255)
                                                                                                                                       tttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTGTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAc                 >  1:550762/1‑150 (MQ=255)
                                                                                                                                         tctcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcat               >  2:3346696/1‑150 (MQ=255)
                                                                                                                                           tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca             >  1:1352638/1‑150 (MQ=255)
                                                                                                                                           tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca             >  2:4847771/1‑150 (MQ=255)
                                                                                                                                           tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca             >  2:907513/1‑150 (MQ=255)
                                                                                                                                            cccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat            >  1:140085/1‑150 (MQ=255)
                                                                                                                                             ccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑ACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACatcatc           >  2:2353961/1‑150 (MQ=255)
                                                                                                                                              cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCACCATCATcc          >  2:3257697/1‑150 (MQ=255)
                                                                                                                                              cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc          >  2:5213195/1‑150 (MQ=255)
                                                                                                                                              cTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATcc          >  1:5188989/1‑150 (MQ=255)
                                                                                                                                               tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg         >  1:4609513/1‑150 (MQ=255)
                                                                                                                                               tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg         >  1:211503/1‑150 (MQ=255)
                                                                                                                                               tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg         >  1:1651069/1‑150 (MQ=255)
                                                                                                                                               tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg         >  1:659220/1‑150 (MQ=255)
                                                                                                                                                     cTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTg   >  1:4373836/1‑150 (MQ=255)
                                                                                                                                                     cTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTg   >  1:2935772/1‑150 (MQ=255)
                                                                                                                                                       tCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgc  >  1:3006570/1‑150 (MQ=255)
                                                                                                                                                       tCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgc  >  2:3932131/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
GCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGG‑‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGC  >  NC_000913/3780982‑3781280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: