Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,362,529 T→C 5.9% intergenic (‑176/+22) yjdQ ← / ← pheU pseudogene, P4‑like integrase remnant/tRNA‑Phe

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,362,5290TC 5.9% 1064.0 / 38.2 323intergenic (‑176/+22)yjdQ/pheUpseudogene, P4‑like integrase remnant/tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base T (162/141);  new base C (13/6);  total (176/147)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 326 total aligned reads displayed.

AGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCA  >  NC_000913/4362380‑4362675
                                                                                                                                                     |                                                                                                                                                  
 gTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTg                                                                                                                                                   >  2:4784544/1‑150 (MQ=255)
    cTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCTAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGTATTGTTTTGGAGGCAGAACGCTTAATTCGTGGCGTCCTGTAACGAAATTCGGACCTCCGTGGAg                                                                                                                                                >  1:4354843/1‑150 (MQ=255)
    cTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAg                                                                                                                                                >  2:360722/1‑150 (MQ=255)
    cTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAg                                                                                                                                                <  2:4139154/150‑1 (MQ=255)
    cTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAg                                                                                                                                                <  2:454835/150‑1 (MQ=255)
     tataGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAgg                                                                                                                                               >  2:5353365/1‑150 (MQ=255)
           tCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGtt                                                                                                                                         >  1:4266752/1‑150 (MQ=255)
            cAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGttt                                                                                                                                        <  2:278930/150‑1 (MQ=255)
             aGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTta                                                                                                                                       <  2:1620325/150‑1 (MQ=255)
             aGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTta                                                                                                                                       >  2:715625/1‑150 (MQ=255)
              ggAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTACATCGTGGCGTCCTGAAACGAAAAACGGACCTCCACGGAGGTCCGttttt                                                                                                                                      <  2:1827613/150‑3 (MQ=255)
               gaactcaTTTTCCAGTATTGGGATATGCCCGCAACTTGCTGGTGATGTGGGGAGTTTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTtata                                                                                                                                     <  2:4917874/144‑1 (MQ=255)
               gAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTtata                                                                                                                                     >  2:1654562/1‑150 (MQ=255)
                  cAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGa                                                                                                                                  <  1:1025/150‑1 (MQ=255)
                   aaaTTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGaa                                                                                                                                 >  1:202315/1‑150 (MQ=255)
                   aaaTTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGaa                                                                                                                                 >  1:1591950/1‑150 (MQ=255)
                    actttgCCCGAATTGGGATATGCCCGCAACTTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAAt                                                                                                                                <  2:5333016/148‑1 (MQ=255)
                       tttCCCGAATTGGGATATGCCCGCAACTGGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTg                                                                                                                             <  2:1493832/150‑1 (MQ=255)
                           ccGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGc                                                                                                                         >  2:808504/1‑150 (MQ=255)
                             gAATTGGGATATGCCCGCAACTTGCGGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGccc                                                                                                                       <  2:4186247/150‑1 (MQ=255)
                                   ggATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTc                                                                                                                 >  2:608709/1‑150 (MQ=255)
                                   ggATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTc                                                                                                                 >  2:1334835/1‑150 (MQ=255)
                                   ggATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTc                                                                                                                 <  2:2336635/150‑1 (MQ=255)
                                   ggATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTc                                                                                                                 >  2:4873064/1‑150 (MQ=255)
                                    gATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCg                                                                                                                >  1:2063980/1‑150 (MQ=255)
                                      tatGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGa                                                                                                              <  2:3029893/150‑1 (MQ=255)
                                        tGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAAt                                                                                                            <  1:5099335/150‑1 (MQ=255)
                                          cccGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCg                                                                                                          <  1:1756825/150‑1 (MQ=255)
                                            cGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGaa                                                                                                        >  1:3304016/1‑150 (MQ=255)
                                            cGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGaa                                                                                                        >  1:3383037/1‑150 (MQ=255)
                                            cGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGaa                                                                                                        >  1:4377349/1‑150 (MQ=255)
                                             gCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAAc                                                                                                       >  2:925110/1‑150 (MQ=255)
                                             gCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTCGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAAc                                                                                                       <  1:109486/150‑1 (MQ=255)
                                              cAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAAcc                                                                                                      >  1:2121538/1‑150 (MQ=255)
                                                  ttGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGTAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAgg                                                                                                  <  1:4497217/150‑1 (MQ=255)
                                                   tGCTGGTGATGTGGGGAGACTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGa                                                                                                 <  2:1074928/150‑1 (MQ=255)
                                                      tGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGacac                                                                                              >  1:2703620/1‑150 (MQ=255)
                                                        gTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACgg                                                                                            >  2:1156243/1‑150 (MQ=255)
                                                        gTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACgg                                                                                            >  1:4997715/1‑150 (MQ=255)
                                                            tgaggggAGAATCTGGTTGAGTTCGGTAGAGTTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGAt                                                                                        >  2:358594/4‑150 (MQ=255)
                                                             gtgGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGAtt                                                                                       <  1:4790472/150‑1 (MQ=255)
                                                                gggAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATTAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTc                                                                                    >  2:1759927/1‑150 (MQ=255)
                                                                gggAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTc                                                                                    >  1:870506/1‑150 (MQ=255)
                                                                gggAGAATCTGGTAGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTc                                                                                    >  1:3470167/1‑150 (MQ=255)
                                                                   agaATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAAt                                                                                 >  2:2504657/1‑150 (MQ=255)
                                                                     aaTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATcc                                                                               <  2:4716191/150‑1 (MQ=255)
                                                                     aaTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAACGGGGATTTTCAATccc                                                                              <  1:896190/150‑1 (MQ=255)
                                                                       tCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATcccc                                                                             <  2:2857677/150‑1 (MQ=255)
                                                                       tCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATcccc                                                                             <  2:3703642/150‑1 (MQ=255)
                                                                         tGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTg                                                                           <  1:3704507/150‑1 (MQ=255)
                                                                         tGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGATTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTg                                                                           <  1:1848823/150‑1 (MQ=255)
                                                                           gTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGGTTATATGAATTTGGTGCCCGGACTCGGACTCGAACCAAGGACACGGGGATTTTCAATCCCCTGct                                                                         >  1:2628762/1‑150 (MQ=255)
                                                                            ttGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGctc                                                                        <  1:4204349/150‑1 (MQ=255)
                                                                              gAGTTCGGTAGAATTGATTCGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTa                                                                      <  1:5216418/150‑1 (MQ=255)
                                                                                   cGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGAc                                                                 >  1:5087185/1‑150 (MQ=255)
                                                                                    ggTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACt                                                                <  1:1133856/150‑1 (MQ=255)
                                                                                     gTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTg                                                               >  2:2173119/1‑150 (MQ=255)
                                                                                      tAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGa                                                              >  1:4135146/1‑150 (MQ=255)
                                                                                         aaTTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCt                                                           <  1:1654562/150‑1 (MQ=255)
                                                                                          aTTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTa                                                          >  1:1438865/1‑150 (MQ=255)
                                                                                            tGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATc                                                        >  2:405197/1‑150 (MQ=255)
                                                                                               tttGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCgg                                                     >  1:991691/1‑150 (MQ=255)
                                                                                                ttGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCAGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCggg                                                    >  1:747615/1‑150 (MQ=255)
                                                                                                     ggCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACg                                               >  2:2651328/1‑150 (MQ=255)
                                                                                                      gCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACgg                                              >  1:2225270/1‑150 (MQ=255)
                                                                                                         gAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGgc                                           <  2:3247752/150‑1 (MQ=255)
                                                                                                         gAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGgc                                           >  2:2405706/1‑150 (MQ=255)
                                                                                                           aCGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGgcgc                                         <  1:1335231/150‑1 (MQ=255)
                                                                                                               ttAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTa                                     >  1:2262556/1‑150 (MQ=255)
                                                                                                               ttAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTa                                     >  1:3637238/1‑150 (MQ=255)
                                                                                                               ttAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTa                                     >  1:2477677/1‑150 (MQ=255)
                                                                                                                    tCGTGGCGTCATGAAACGAAAAACGGACATCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGCGATTTTCAATCCCCTGCTCTACCGACTGAGCTCTCCGGGCAACGGGGCGCATTAAACCg                                >  2:3108153/1‑150 (MQ=255)
                                                                                                                       tGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTaa                             <  2:1333019/150‑1 (MQ=255)
                                                                                                                         gCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATc                           >  1:5337630/1‑150 (MQ=255)
                                                                                                                           gTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTg                         <  2:4997715/150‑1 (MQ=255)
                                                                                                                           gTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTg                         <  1:400773/150‑1 (MQ=255)
                                                                                                                           gTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTg                         <  2:4623378/150‑1 (MQ=255)
                                                                                                                            tCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGc                        >  1:3575488/1‑150 (MQ=255)
                                                                                                                              cTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGACTCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCAGTAAACCGTAATCTGcac                      >  2:2910875/1‑150 (MQ=255)
                                                                                                                                 aaaCGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTAAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACAtc                   >  1:1939913/1‑150 (MQ=255)
                                                                                                                                   aCGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGCATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACAtctc                 <  2:3765412/150‑1 (MQ=255)
                                                                                                                                     tataaaCGGACCTCCACGGAGGTCCGTGTAGATGCATTTGGTGCCCGGACTCGGAATCGTACCAAGGACACGGGGAGTTTCCCTCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAGACCGTAATCTGCTCATCTCGt               >  2:5427671/4‑150 (MQ=18)
                                                                                                                                     gAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGt               >  2:1939405/1‑150 (MQ=255)
                                                                                                                                     gAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACAGAGCTATCCGGGCAACGGGGCGCCTTAAACCGTAATCTGCACATCTCGt               >  1:3063653/1‑150 (MQ=255)
                                                                                                                                       aaaaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCa             >  2:4462120/1‑150 (MQ=255)
                                                                                                                                       aaaaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCa             <  1:570644/150‑1 (MQ=255)
                                                                                                                                         aaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAc           >  1:4888722/1‑150 (MQ=255)
                                                                                                                                         aaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAc           >  1:5207908/1‑150 (MQ=255)
                                                                                                                                         aaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAc           >  1:2874225/1‑150 (MQ=255)
                                                                                                                                         aaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAc           <  1:1309427/150‑1 (MQ=255)
                                                                                                                                         aaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAc           >  1:5208024/1‑150 (MQ=255)
                                                                                                                                         aaCGGACCTCCACGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAc           >  1:1913283/1‑150 (MQ=255)
                                                                                                                                         aaCGGACCTCCACGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAc           >  1:2609228/1‑150 (MQ=255)
                                                                                                                                         aaCGGACCTCCACGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATACGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAc           >  1:2322508/1‑150 (MQ=255)
                                                                                                                                          aCGGACCTCCACGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAAcc          >  1:5014975/1‑150 (MQ=255)
                                                                                                                                             gACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTaa       <  1:4596028/150‑1 (MQ=255)
                                                                                                                                              aCCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAAt      <  1:168535/150‑1 (MQ=255)
                                                                                                                                                  ccGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCa  >  1:3823597/1‑150 (MQ=255)
                                                                                                                                                  ccGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCa  <  2:5087185/150‑1 (MQ=255)
                                                                                                                                                  ccGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCa  <  1:4784544/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCA  >  NC_000913/4362380‑4362675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: