Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 915,226 T→G 10.0% intergenic (‑369/+126) ybjE ← / ← aqpZ putative transporter/aquaporin Z

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913915,2260TG10.0% 406.3 / 5.0 188intergenic (‑369/+126)ybjE/aqpZputative transporter/aquaporin Z
Reads supporting (aligned to +/- strand):  ref base T (121/23);  new base G (6/15);  total (146/42)
Fisher's exact test for biased strand distribution p-value = 4.49e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

CATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGT  >  NC_000913/915077‑915375
                                                                                                                                                     |                                                                                                                                                     
caTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCt                                                                                                                                                       <  2:2129352/150‑1 (MQ=32)
 aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTg                                                                                                                                                      >  2:1840967/1‑150 (MQ=32)
 aTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTg                                                                                                                                                      >  1:4714979/1‑150 (MQ=32)
    gATGTGGGCTGAGGGCGCGTTTTACAGGGATGGTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTgcgc                                                                                                                                                   <  2:1319760/150‑1 (MQ=17)
        tgGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAATGGCCGCCCCCTGCGCGCTc                                                                                                                                               >  2:4967972/1‑150 (MQ=17)
         gggCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTcc                                                                                                                                              >  2:607030/1‑150 (MQ=32)
           gCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTccat                                                                                                                                            >  2:5066576/1‑148 (MQ=17)
                gggCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGcc                                                                                                                                       >  2:360830/1‑150 (MQ=32)
                   cgcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGt                                                                                                                                    >  1:3487727/1‑150 (MQ=17)
                       ttttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCa                                                                                                                                >  1:4289799/1‑150 (MQ=34)
                         ttACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAat                                                                                                                              >  2:373632/1‑150 (MQ=35)
                          tACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCCGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAata                                                                                                                             >  2:884988/1‑150 (MQ=255)
                           aCAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAatat                                                                                                                            >  1:436874/1‑150 (MQ=38)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCATCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                          >  1:5029389/1‑150 (MQ=255)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                          >  2:5003891/1‑150 (MQ=39)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGt                                                                                                                          >  1:1016264/1‑150 (MQ=39)
                             aGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCACCTTGCGCCAGTGGCAATATGt                                                                                                                          >  2:4117344/1‑150 (MQ=255)
                                   gTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTa                                                                                                                    >  2:3923514/1‑150 (MQ=255)
                                    ttACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAg                                                                                                                   >  1:3447142/1‑150 (MQ=255)
                                       ccTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTc                                                                                                                >  1:2974284/1‑150 (MQ=255)
                                        cTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                               >  2:1068712/1‑150 (MQ=255)
                                        cTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                               >  2:3297655/1‑150 (MQ=255)
                                        cTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCa                                                                                                               >  2:1395131/1‑150 (MQ=255)
                                         tCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCAt                                                                                                              >  2:1020566/1‑150 (MQ=255)
                                          cgcgCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCAGTGGCAAAATGTTGCTTAGCTCATg                                                                                                             >  1:3717509/1‑150 (MQ=255)
                                            cgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCGCCGAGCGCCCGGGGCAATATGTTGCTTAGCGCaggga                                                                                                           >  1:4135656/1‑146 (MQ=14)
                                              cccGACCCGGTCGCCGTAAGGGATCCGTCTGGGGCCCCGGGCTGGGGCGTTTTTGTTCGCCAGTGCCGGGGGGTGCTGGGGGCGCCGGCTATGGGCCGCCCCCCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAg                                                                                                         <  2:4004795/150‑1 (MQ=255)
                                               ccGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCACTGGCAATATGTTTCGTAGCTCATGAAAgg                                                                                                        >  1:2498401/1‑150 (MQ=255)
                                                  aCCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAgc                                                                                                     >  1:1679598/1‑150 (MQ=255)
                                                    ccGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAgcgc                                                                                                   >  2:5307354/1‑150 (MQ=255)
                                                     cGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCACCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGca                                                                                                  >  2:223208/1‑150 (MQ=255)
                                                      gggcgcggTAGGGGATTGGTGGGGGGCCCGGCGCGGCGGGGATTTTGGGCGCCCGCGCCGGGGGGTGCTGGGGGGGGGGGCGTTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGcaa                                                                                                 <  2:867447/143‑1 (MQ=255)
                                                            cGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgat                                                                                           >  2:4310678/1‑150 (MQ=255)
                                                              tAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCGGCGCGCTCCTTTCGCCAGGGGCAATAAGTTGCTTAGCGCATGAAAGGAGCGCCACAAgatga                                                                                         >  1:770085/1‑150 (MQ=17)
                                                               acgggATATGCCGCGGGCACCGCCCCGCGGCTTTTTTGTGCGCCAGCGCCGGGGGGGGCGGGGGGCGGCGGGTATGGGCCGCCCCGTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAgatgat                                                                                        <  2:4870454/148‑1 (MQ=17)
                                                                aGGTATACGTCGCGGGCACGGCGCCGCGGGGATTTTGTTCGCCAGCGCCGGGGGGTGCTGGGGGGGGCGGGTTTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGatgatg                                                                                       <  2:2715027/150‑1 (MQ=18)
                                                                  ggATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGCTTGGCCGCCCCCTGCGCGCTCCGTGCGCCCGGGGCCATATTTTGCTTAGCTCCTGGAAGGGGCGCAACAAGATGATGaa                                                                                     >  1:694567/1‑150 (MQ=17)
                                                                   gATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCCCCCCCAGCGCGCGCCATGCGCCACTGGCAAAAATTTTCTTAGCTCAAGAAAGGAGCGCAACAAAAAGATTaaa                                                                                    >  1:5435808/1‑149 (MQ=17)
                                                                     tAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGGTTGGCCCCCCCCAGCGCGCGCCTTTCGCCCGTGGCAATAAGTTGCTTAGCTCATGTAAGGAGCGCAACAAGATGAAGAAtaa                                                                                  >  1:4971873/1‑148 (MQ=18)
                                                                      atgtggtgggCCCCGCGCTGGGGGTTTTTTGTGCGCCTGTGCCGGGGGGTGCTGGGGGGGGGGGGTTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCaa                                                                                 <  2:3511338/143‑1 (MQ=18)
                                                                        gtggtgggCACGGCGCCGGGGGTTTTTTGTTCGCCTGAGCCGGGGGGTGCTGGGGGGGGGGGCTTTTGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACa                                                                               <  2:805920/145‑1 (MQ=18)
                                                                         tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                              >  2:5404568/1‑150 (MQ=255)
                                                                         tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACat                                                                              >  2:1982119/1‑150 (MQ=255)
                                                                         tCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGAGTTGCCGCCCCCAGCGCGCTCCGTGCGCCAGTGGCCAAATTTTTGGTTGCTCATGAAAGGAGAGCAAAAAAAAGTGGAATCAACat                                                                              >  1:55058/1‑150 (MQ=14)
                                                                           gAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTTGCCGCCCCCAGCGCGCTCCATGCGCCCGTGGCAAAAATTTGCTTAGCTAAAGAAAGGAGCGCAACAAGAATATGAATCAACaaat                                                                            >  1:3633592/1‑150 (MQ=17)
                                                                            aGGGCACCGCGCAGCGGCGAGTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCGCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACagatc                                                                           >  2:1788770/1‑149 (MQ=255)
                                                                             gggCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaa                                                                          >  2:4481132/1‑150 (MQ=255)
                                                                                cACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCCGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaacaa                                                                       >  2:492716/1‑150 (MQ=255)
                                                                                 aCCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaacaac                                                                      >  2:3541777/1‑150 (MQ=255)
                                                                                   cgcgCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATTAATCAACATATAACAACAt                                                                    >  2:3780797/1‑150 (MQ=255)
                                                                                          cggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaa                                                             >  2:4986704/1‑150 (MQ=255)
                                                                                          cggcgATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTaaaa                                                             >  2:771664/1‑150 (MQ=255)
                                                                                              gtttttGTTCGCCCGCGCCGGGGGGTGCTGGGGGGGGCGGCGAGTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAg                                                         <  2:3078721/148‑1 (MQ=25)
                                                                                              gagtttGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCAGCGCGCTCCTTGCGCCCGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCGTAAAAAAAg                                                         >  2:2856375/4‑150 (MQ=255)
                                                                                                     ttCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAACATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCAGAc                                                  >  2:4396057/1‑150 (MQ=255)
                                                                                                     ttCGACAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCAAGAAAGGAGCGCAACAAGATGATGAATCAACAAATAACAACATCTTAAAAAAATGCCTGAc                                                  >  2:3245935/1‑150 (MQ=255)
                                                                                                         ccAGCGCCGGGGGGTGCGGGGGGCGGCGGCGTTGGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTa                                              <  2:3434757/150‑1 (MQ=37)
                                                                                                         ccAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTa                                              >  2:4916361/1‑150 (MQ=255)
                                                                                                         ccAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTa                                              >  2:2791346/1‑150 (MQ=255)
                                                                                                         ccAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTa                                              >  2:4725343/1‑150 (MQ=255)
                                                                                                                    gggggggggggggggggGCGTGGGGCCCCCCCCGCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCtt                                   <  2:1606307/143‑1 (MQ=255)
                                                                                                                    gggTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCtt                                   >  2:3844883/1‑150 (MQ=255)
                                                                                                                      gTGCTGGGGGGGGGGGCTATGGGCCCCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCt                                 <  1:3038292/150‑1 (MQ=38)
                                                                                                                            ggggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtta                           >  2:2963910/1‑150 (MQ=255)
                                                                                                                            ggggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGtta                           >  2:2179585/1‑150 (MQ=255)
                                                                                                                             gggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaa                          >  2:430306/1‑150 (MQ=255)
                                                                                                                             gggCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaa                          >  1:1111383/1‑150 (MQ=255)
                                                                                                                              ggcggggggGTTGGGCCGCCCCCGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaat                         <  1:2925534/150‑1 (MQ=255)
                                                                                                                               gcggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatt                        >  2:1189146/1‑150 (MQ=255)
                                                                                                                               gcggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatt                        >  2:1189162/1‑150 (MQ=255)
                                                                                                                                 ggcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaattaa                      >  1:5265345/1‑150 (MQ=255)
                                                                                                                                  gcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGACTCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTtaattaat                     >  2:582382/1‑150 (MQ=255)
                                                                                                                                  gcggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTtaattaat                     >  2:5445037/1‑150 (MQ=255)
                                                                                                                                   cggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATc                    >  2:1702094/1‑150 (MQ=255)
                                                                                                                                    ggcgTTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCa                   <  2:4934518/150‑1 (MQ=255)
                                                                                                                                    ggcgATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCa                   <  2:4934635/150‑1 (MQ=255)
                                                                                                                                       gtgtGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc                <  2:2650889/147‑1 (MQ=255)
                                                                                                                                       gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc                >  2:1542776/1‑150 (MQ=255)
                                                                                                                                       gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc                >  1:347342/1‑150 (MQ=255)
                                                                                                                                       gATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc                >  2:2195833/1‑150 (MQ=255)
                                                                                                                                       gATGGGCCGCCCCCCCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGc                <  1:119405/150‑1 (MQ=255)
                                                                                                                                          gggCCGCCCCGGGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCttt             <  1:3357428/149‑1 (MQ=255)
                                                                                                                                            gccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc           >  2:4836870/1‑150 (MQ=255)
                                                                                                                                            gccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc           >  1:2686778/1‑150 (MQ=255)
                                                                                                                                            ccccccccccccGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTc           <  1:5181840/146‑1 (MQ=255)
                                                                                                                                             ccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTcc          >  1:447110/1‑150 (MQ=255)
                                                                                                                                             ccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTcc          >  2:2433514/1‑150 (MQ=255)
                                                                                                                                             ccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTcc          >  1:1813642/1‑150 (MQ=255)
                                                                                                                                             ccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTcc          >  2:855046/1‑150 (MQ=255)
                                                                                                                                             ccgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTcc          >  1:2863870/1‑150 (MQ=255)
                                                                                                                                              cgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCca         >  1:2364205/1‑150 (MQ=255)
                                                                                                                                              cgccCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCca         <  2:3388082/150‑1 (MQ=255)
                                                                                                                                              ccccccccGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCca         <  1:2214137/148‑1 (MQ=255)
                                                                                                                                                cccccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACACATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagc       <  1:3174075/150‑1 (MQ=255)
                                                                                                                                                cccccTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagc       <  2:3819887/150‑1 (MQ=255)
                                                                                                                                                 ccccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagca      >  2:3957296/1‑150 (MQ=255)
                                                                                                                                                 ccccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagca      >  2:3957397/1‑150 (MQ=255)
                                                                                                                                                  cccTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCcagcag     <  1:209458/150‑1 (MQ=255)
                                                                                                                                                   ccTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAgg    >  1:4912913/1‑150 (MQ=255)
                                                                                                                                                     tGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGt  >  2:224373/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGT  >  NC_000913/915077‑915375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: