Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 164,581 | A→C | 15.3% | intergenic (+47/‑149) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,581 | 0 | A | C | 15.3% | 195.3 / 10.8 | 116 | intergenic (+47/‑149) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base A (27/69); new base C (2/18); total (29/87) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.53e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 120 total aligned reads displayed.
CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA > NC_000913/164432‑164730 | ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa < 2:2719185/150‑1 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt < 2:4142420/150‑1 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc > 2:3782485/1‑150 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc > 2:1984728/1‑150 (MQ=255) tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCcgcgcg > 1:2490508/1‑150 (MQ=255) atgtgaTGAAAGGGCGTTATCCCAAACTTGTCTGGCGGGACGACCCGGAAAAT‑ACTGCACCGACGAGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGtt < 2:2700444/146‑1 (MQ=38) aaGAGATGATTGGGCTTTATCCCAAACTTGTGGGGCCGTACGACCGGGAAAAT‑ACTGCACCGACGCGACGGACTAAAATGTTTTCTTATGTTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGTTGTGGGCATCAGCGCGCACGtt < 2:3866992/150‑1 (MQ=21) aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCCCggg > 1:9558/1‑148 (MQ=255) aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtg > 2:3912156/1‑149 (MQ=255) agaTGACCGGGCGTTATCCCCAACATGTCTGGCGGGACGCCCCGGCACAT‑ACTGCACCGAGGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcac < 2:3055815/150‑1 (MQ=37) agaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtggcc > 1:2003930/1‑146 (MQ=255) tattaatgggCGTTATCACAAACTTTTGTGGCCGGACGACCCGGCAAAA‑ACTGCACCGAGGCGCCGTACTAAATTTTTTTCTTTTGGTTGTTTTTCCCCCTCCCCGTGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcacc < 2:3785614/143‑1 (MQ=255) gatgatTGGGCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGAGGGACGAAAAAGTTTTCGTACGGTTGGTTTTCGCCCTGTCCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTtcacc < 2:893818/150‑1 (MQ=255) gaTGCACGGGCTTTATCCCAAACTGGTCTGGCCGGACGACCCGGAAAAT‑CCTGCACCGCCGCGACGGACGAAAAAGTTTTCGTATGGTTGGTTTTCTCCCTCTCCGTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTtcacc < 2:5310542/150‑1 (MQ=255) tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgggcccg > 1:1911418/1‑143 (MQ=255) aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctca > 2:3968180/1‑150 (MQ=255) acgggCGTTATCCCAATCTTGTCGGGCCGGACGCCCCGGCAAAT‑ACTGCCCCGACGCGAGGGACTAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGGGGTTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTtcaccctcac < 2:4180123/148‑1 (MQ=255) tgggCTTTAACCAAAACTTGTCTGGCCGGACGACCCGGAAAAT‑ACTGCACCGACGCGACGGACTAAAATTTATTGTTAGGGTTGGTTTACCCCCACGCCCTGTGGGTTTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcaccctcacc < 2:2102292/149‑1 (MQ=255) cgggCTTTACCCCAAACAGGTCTGGCCGGACGACCCGCCAAAT‑ACTGCCCCGACGCGCCGGACGAAAATTTTTTGTTTTGGTTGGTTTTCTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 2:2109126/149‑1 (MQ=25) gTTACCCCAATCTTGTCGGGCGGGCCGA‑CCGGTAAATGCCGGCCCCGACGGGACGTACTAAAATGTATTGGTAGGGTTGGTTTTCTCCCCCTCCCTGTGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTatc < 2:4210093/150‑3 (MQ=255) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGTCCCACACCCaaaac > 1:965308/1‑148 (MQ=38) aacccAAACATCACTGCCCGCACGACCCCCAAAAA‑ACTCCACCCACCCCACGCACGAAAAACTATTCGTAAGGTTGCTTTACACCCACACCCTCTGGGACAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCct < 2:3207817/148‑1 (MQ=255) cAAACATGTCTGGCCGGACGACCCGGAAAAT‑ACGGCACCGACGCGACGGACGAAAAAGTATACTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:2774557/150‑1 (MQ=38) ttttCTGGCCGGCCCCCCCGCAAAAT‑AGTGCACCGCCGCGAGGTACCAAAATTTATTCTTACTTTTGTTTTCCCCCCCCCCCGTGGGGGTGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 1:1720012/147‑1 (MQ=255) ttGTCGGGCCGGACGACCCGGAAATT‑ACTGCACCGAGGCGACGTACTAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCCCCCTGGGGTTGAGGGCCGGGTTTAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:1773130/149‑1 (MQ=255) atgtggggCCGGACGACCCGGCAAAT‑ACGGCACGGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:4376797/144‑1 (MQ=38) aTGTCGGGCCGGACGACCCGCCAAAT‑ACTGCCCCGACGCGCCGTACGAAAATGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGGGGGCTCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:4376748/150‑1 (MQ=255) ttgggggCCGGACGCCCCGGAAAAT‑CCTGCACCGCGGCGAGGGACGAAAATGTTTTCGTTGGGTTGGTTTTCGCCCTCTCCCTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 1:2648209/145‑1 (MQ=25) tGTCGGGCCGGACGACCCGGAAAAT‑ACTCCACCTACGCGACGGACTAAAATGTATTCGTATGGTTGGTTTTCCCCCTCTCCCTTTGGGATTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:3893837/150‑1 (MQ=37) tCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCCAACCCACAccccccacgg > 2:641800/1‑143 (MQ=37) tGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGGACCCGCACCCGAAACCACGCCcacaccggcg > 1:3001512/1‑144 (MQ=37) tGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCGAACCCTCTCCCGCCAGGgag > 2:792226/1‑150 (MQ=255) tGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCAAACCCGCTCCCCCAAgggtg > 1:4397130/1‑148 (MQ=37) tGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGAGCCCACACCCAAACCCACTCACACAAgggtg > 1:3001511/1‑148 (MQ=37) gCCGGACGACCCGGAAAAT‑ACTGCACCGCCGCGACGGACGAAAATTTTTTCGTATGGTTGGTTTCCCCCCCCTCCCTGGGGGAGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 1:4899699/150‑1 (MQ=255) ccGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 2:5427290/150‑1 (MQ=255) ccGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 2:897620/150‑1 (MQ=255) ccGGACGACCCGCAAAAT‑ACGCCACGGACGCGCGGGACTAAAATTTTTTCGTCTGGTTGTTTTTACCCCGCGCCCTGTGGGAGCGGGGGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg < 1:5226318/150‑1 (MQ=255) cGGCCGCCCCGGCAAAT‑CCGCCCCCGCCCCGCCGGAGGAAAACGTCTTCGTACGGTGGGTTTCCTCCCTCGCCCGGGGGGCGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 2:2922165/150‑1 (MQ=255) cGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCCCACCCAAACCCTCTCCCGCAAGGGAGgggg > 2:4135861/1‑150 (MQ=38) cGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGGACGGGTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGGGCGCACGGTCACCCTCACCCGCACCCTCTCCCGCCCGGGAGAggg > 2:4135392/1‑150 (MQ=37) gccgccccGGAAAAA‑AGTGCACCTAGGCGCCGGCCTAAAATTTTTTCTTATGGTTGTTTTTCTCCCTCTCCGGGGGGGTGTGGGCGGGGTTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:1139393/145‑1 (MQ=255) ccGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCCCACCCAAACCCCCCCCCCAAAGGGGGTGGGGGcccaac > 2:1710160/1‑146 (MQ=25) gCAAAG‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:5042921/150‑1 (MQ=255) aaaT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGGCCCACCCCCAAACCCCCGCCCACACGGGGGGGGGGGCCCCTCGAGcc > 2:2603040/1‑149 (MQ=21) aaT‑ACGGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGGTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGTGAGGGGACCGATCGAGcac < 2:3530845/150‑1 (MQ=255) cTGCACCGACGCGACGGACGAACAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAt < 2:823151/150‑1 (MQ=255) tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGAGCCACACCCCAAACCACACCCACCACGGGGAGGGGCCCGCTCGGGCCCaaaat > 1:809734/1‑148 (MQ=21) tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACAACCAAACCCACTACCACCAGGGAGGGGGGCCCGGGAGAGCACAAAta > 2:266111/1‑149 (MQ=21) cACCGACGCGACGGACTAAAATGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:543414/150‑1 (MQ=255) aCCGACGCGACGTACCAAAATGTTTTCGTATGTTTGTTTTCCCCCCTCCCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTg < 2:5149604/150‑1 (MQ=38) ccGACGCGACGGACTAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:3968700/150‑1 (MQ=255) ccGACGCGACGGACGAAAAATTTTTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:4555868/150‑1 (MQ=38) ccGACGCGACGGACGAAAAAGTATTCGTAAGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:4455943/150‑1 (MQ=255) cgacgcgaggGACGAAAATGTTTTCGTACGGTTGGTGTTCTCCCTCCCCCGGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag < 1:1238378/150‑1 (MQ=38) gaggcgacgGACGAAAATGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 2:2662313/147‑1 (MQ=255) acgcgccgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag < 2:3929264/150‑1 (MQ=255) aattATTCGTAAGGTTGGTTTTCTCCCCCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:5466108/147‑1 (MQ=255) gTATTCGTATGGTTGGTTTTCTCCCTCTCCGTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:2209148/150‑1 (MQ=255) tCGTATGTTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 2:3026337/150‑1 (MQ=255) atgtttggttTCCTCCCTCCCCGTGGGGTGGTGGGCCGGGTTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:2165767/146‑1 (MQ=255) aatgttggttTTCCCCCTCTCCCTGTGGGAGAGGGCCGTCGTGAGGGCATCACCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGCTGACCGATCGAGCACAAATTTAGAGAGATATATTCTTCTGTCTTGTAACaca < 1:1830041/147‑3 (MQ=38) tggttggttTTCCCCCCCTCCGGGGGGGTGCGGGCGGGGTGGTGGGACTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 2:9558/149‑1 (MQ=255) aGGTTGGTTTTCACCCTCACCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:4788362/150‑1 (MQ=255) tggttTTCCCCCTCGCCGTGTGGGTGCGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 1:304733/150‑1 (MQ=255) ctccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCGAGCCCAAACCCACACCCAACAAGGAGAGGGGCCCGCTCGAGCACAAATGTTGAGAGAAATCATATTCAGTGTTGTAACAGAACAACAGaaaaa > 2:2285069/1‑149 (MQ=16) tccctctccgtGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:1760805/150‑1 (MQ=255) tcccccccccgGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:2030071/150‑1 (MQ=255) cccccccccctGCGGCAGAGGCCCGGGGAGCGCCCCTCAGCGCCCACGTTCACCCACACCCTAACCCTCACCCACAACGGAGAGGGGACCGATCGAGCACAAAATTTGAGAGATATCTTCTTCTGACTTGTAACAGAAGAACAGAAAATc < 2:1208798/143‑1 (MQ=255) ccctctccgtGTGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 1:2252226/150‑1 (MQ=255) ccctctccctGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 1:2252437/150‑1 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg < 2:3479033/150‑1 (MQ=255) tcgcccgGTGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGc < 1:3949615/147‑1 (MQ=255) cctGGGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtttt < 1:1216789/150‑1 (MQ=255) ccacagggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCAAACCCACACCCCCAAGGGGGCGGGGCCCCAAAGAGCCCAAAAGTTTAGAGATAAAATAGTCTGTCTTGTAAAACAATAACAGCAAAAAGGGCtttt > 2:1800096/6‑150 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc < 2:3501762/150‑1 (MQ=255) tgggggAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc < 2:5175163/147‑1 (MQ=255) tgGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:3444659/150‑1 (MQ=255) ggggAGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 2:3931816/149‑1 (MQ=255) gggggggggCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGcc < 2:924817/144‑1 (MQ=255) ggtgtgggCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 2:1029852/145‑1 (MQ=255) ggcgagGGCGGGGGTGTGGGCCTCATCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACAGATCGAGCACAAATTTTGAGAGATATCTTCGTCTGTCTTGTAACAGCAGAACAGAAAATCGGGCTTTTGCGCCt < 1:496026/147‑1 (MQ=255) ggAGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 2:129068/150‑1 (MQ=255) ggAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:496067/150‑1 (MQ=255) gagcGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTg < 2:4274172/150‑1 (MQ=255) agagGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGa < 2:5325084/150‑1 (MQ=255) gtgggCCGGGTGGCGGGCATCAGCGCGCACGTTCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGaa < 2:4831338/148‑1 (MQ=255) cgggCCGGGGGGAGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAat < 1:1324637/149‑1 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACACCCACAAGGGAGTGGGGGCCGCTAGTGCCCAAAAATTTAGTGATATAATATTAAGTGTTGTTAAAGAAGTACACAAAATCGGGCGTTTTCGCCGGAatat > 2:2746333/1‑150 (MQ=255) gggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:4808749/149‑1 (MQ=255) cGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACCGAAAATCGGGCTTTTGCGCCTGAATATTGc < 2:3770657/150‑1 (MQ=255) ggggTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:4376681/150‑1 (MQ=255) ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:2603040/150‑1 (MQ=255) ggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:196616/150‑1 (MQ=255) ggTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCAATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:1523559/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:3491620/150‑1 (MQ=255) gCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAg < 1:437586/150‑1 (MQ=255) aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCa < 2:3324774/150‑1 (MQ=255) aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCa < 2:446817/150‑1 (MQ=255) aTCAGCGCGCACGTTCACCCACAACCAAAACCAAAACCACAAGGGAGAGGGGACCCCTAGAGCCCAAAATTTTAGAGAAATAATATTATGTCTTGTAACAGAAGAACACAAAAACGGGCATTTGCGCCAGAAAATTTCTGATAAAAAGCa > 2:2284516/1‑150 (MQ=255) | CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA > NC_000913/164432‑164730 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |