Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 14.6% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA14.6% 594.1 / 14.7 260intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (99/108);  new base A (38/8);  total (144/116)
Fisher's exact test for biased strand distribution p-value = 1.44e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177085‑2177383
                                                                                                                                                     |                                                                                                                                                       
gTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc                                                                                                                                                         >  2:3342068/1‑150 (MQ=255)
gTTGGCAGCGGTTTCTACCACCACTTGCATCGGTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTc                                                                                                                                                         >  2:4894218/1‑150 (MQ=255)
 ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGTCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                        >  2:2571725/1‑150 (MQ=255)
 ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                        <  1:921176/150‑1 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCGGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                                   <  2:3771540/150‑1 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                                   <  1:463050/150‑1 (MQ=255)
         ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                               <  2:2873015/150‑1 (MQ=255)
             tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                           <  2:3648348/150‑1 (MQ=255)
             tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                           <  1:707681/150‑1 (MQ=255)
              cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                          <  1:4105766/150‑1 (MQ=255)
               tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAat                                                                                                                                         >  2:1628110/1‑150 (MQ=255)
                 ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                       >  2:1839376/1‑150 (MQ=255)
                  caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAattat                                                                                                                                      >  1:4779869/1‑150 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                     <  2:4081585/150‑1 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                     <  1:4072526/150‑1 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTAAaatatt                                                                                                                                     >  1:1904369/1‑150 (MQ=255)
                    ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                    >  1:259363/1‑150 (MQ=255)
                    ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                    <  1:5207431/150‑1 (MQ=255)
                    ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                    <  2:3151863/150‑1 (MQ=255)
                    ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATTATTAAATTAttt                                                                                                                                    >  1:4208608/1‑150 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                <  2:3110828/150‑1 (MQ=255)
                           cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                             <  1:5101842/150‑1 (MQ=255)
                           cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                             <  2:2362512/150‑1 (MQ=255)
                           cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                             <  1:2299337/150‑1 (MQ=255)
                            aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                            <  2:2982035/150‑1 (MQ=255)
                             tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTaaa                                                                                                                           >  2:5195627/1‑150 (MQ=255)
                             tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAATGAAATTATTTTTTTTTaaa                                                                                                                           >  1:4269135/1‑150 (MQ=255)
                              cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATTAAATTATTTTTTTTTAAat                                                                                                                          >  1:5465466/1‑150 (MQ=255)
                                 ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                       <  1:1755374/150‑1 (MQ=255)
                                 ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATAAAATGAAATTATTTTTTTTTAAATATc                                                                                                                       >  2:134889/1‑150 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                      <  1:3456331/150‑1 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                      <  1:3402598/150‑1 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                      <  2:5262429/150‑1 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGATTTTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTAAATATAgaa                                                                                                                    >  1:3794415/1‑149 (MQ=255)
                                        ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                               <  2:4810094/150‑1 (MQ=255)
                                           tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                            <  1:2072658/150‑1 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           >  1:3136049/1‑150 (MQ=255)
                                               tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                        >  1:3615735/1‑150 (MQ=255)
                                                 ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                      <  2:2277533/150‑1 (MQ=255)
                                                 ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAAAGAAATTATTTTTTTTTAAATATAGAGATAACGATAACaa                                                                                                      >  2:1125390/1‑150 (MQ=255)
                                                 ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGAGTTCAAAATAAAATGAAAATATTTGTTTTTAAATATAGAGATAACGATCCCaa                                                                                                      >  1:1085366/1‑150 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATAGAGATAACGATCACaaaaa                                                                                                   >  1:1771661/1‑150 (MQ=255)
                                                           gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAATAATGAAATTATTTTTTTTTTAATATAGAGATAAAGATCACAAAAAAGACaaa                                                                                            >  1:629964/1‑149 (MQ=255)
                                                            aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTTAATATAGAGATAACGATCACAAAAAAGACAata                                                                                           >  1:3168065/1‑150 (MQ=255)
                                                              ccGCATAACCGCCGCGCTGTGCGTTGTTCAGAATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATg                                                                                         <  2:3953786/150‑1 (MQ=255)
                                                                 cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCAAAAAAACGACAAAATGaaa                                                                                      >  2:4384045/1‑150 (MQ=255)
                                                                 cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                      <  2:815891/150‑1 (MQ=255)
                                                                  aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAATTATTTTTTTTTAAATATAGAGGTAACGATAACAACAAAGACAATATGaaaa                                                                                     >  1:1481156/1‑150 (MQ=255)
                                                                  aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGAATTAAAAATATAATTTAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAACGACAAAATGtaaa                                                                                     >  1:153695/1‑150 (MQ=255)
                                                                   tAACCGCCGCGCTGTGAGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTATCAAATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATTAAATTATTTGTTTTTAAATATAGAGATTCCGATCACAAAAAAGACAGAATGAGAat                                                                                    >  2:3284295/1‑150 (MQ=255)
                                                                    aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATTTGTTTTTTAATATCGAGAGAAACATCACAAAAACGACAAAATTaaaaat                                                                                   >  1:4432796/1‑148 (MQ=255)
                                                                      ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATAGAGATAAAGATCACAAAAACGACAATATGAAAattat                                                                                 >  2:717654/1‑150 (MQ=255)
                                                                       cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                >  2:2203994/1‑150 (MQ=255)
                                                                         ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGAATTAAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAAGAAAAAATGAAAATTAATCg                                                                              >  2:4708861/1‑150 (MQ=255)
                                                                           gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAAATAAATTAATTTTTTTTAAATAAATAGATAAACAAAACAAAAAAAACAAAATTAAAAATAATagag                                                                            >  1:1482328/1‑150 (MQ=255)
                                                                             gcggtgCGTGTTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTTTGGTTTGTTTTCGTTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAGGTTCACAAAAACGACAATATGAAAATTATTCgagga                                                                          <  2:4142874/147‑1 (MQ=255)
                                                                               tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGATAATTATTCGAGGAGt                                                                        <  2:3439891/150‑1 (MQ=255)
                                                                                  gCGTTGTTCAGAATCTGATTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATATTGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                     <  2:1502641/150‑1 (MQ=255)
                                                                                   cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAACCATCACAAAAACGACAATATGAAAATTATTCGAGGAGTTaaa                                                                    >  2:5331142/1‑150 (MQ=255)
                                                                                   cGTTGTCCAGCATCTGCTTTGACGAAACCACTTACATTTTCATATCCTGTCTTTTGTTTTCTTTTACAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                    <  1:3325259/150‑1 (MQ=255)
                                                                                        ttCAGATTCTGCTTTGTCTATACCACTTAAATTTTCATCTCCTGTGTTTTTTTTTCTATTTAAAAATATAATCAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                               <  1:1125490/150‑1 (MQ=255)
                                                                                         acAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  2:4211770/149‑1 (MQ=255)
                                                                                             cttCTGCTTTTTCTATACCACTTACATTTTCATATCCTGTCTTTTTTTTTCTTTTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCGAGATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  1:2659829/148‑1 (MQ=255)
                                                                                              aTCTGCTTTGTCTATACCACGTACATTTTAATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATGAAATTTTTTTTTTTTAAATATCTAGATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGCAGTGAAAGGCAAAAAAAc                                                         <  1:429129/150‑1 (MQ=255)
                                                                                              aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGATTTTAAAAAATAAATTAAATATTTTTTTTTTTAAAAAGACATAAACATGAAAAAAAAAAACAAATGTAAAATATTAGAGGATTGTAATGCcaaaaaaa                                                         >  1:2613865/1‑149 (MQ=255)
                                                                                              aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGTATTTTAAAAAATATATTAAATATTTTTTTTTTAATATCGTGAGTAAGAACACAAAAACCACAAAATTTAAAATATTAGAGGGGTGTAAGGGCAAAAAAc                                                         >  1:2475670/1‑150 (MQ=255)
                                                                                                 tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGc                                                      >  2:1641189/1‑150 (MQ=255)
                                                                                                 tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTTAAAAAATAAATAAAATATTTTTTTTTTAATATAGAGATAAAGAAAAAAAAAAAAAAAAAAAGAAAAATATTAGAGGGGGGTAAGGCaaaaaaaaagc                                                      >  1:2374895/1‑146 (MQ=255)
                                                                                                     ttGTCTATCCCACGTACATTTTAATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                  <  2:4694588/150‑1 (MQ=255)
                                                                                                     ttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                  <  2:1177019/150‑1 (MQ=255)
                                                                                                        tCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAAAATAATGAAAATATTTTTTTTTTAATTTAGATGTTAACATCACAAAAAACACAATATGAAAATTTTTTGAGGTGTGTAAGGCCAAAAAAACGCCAccccg                                               >  2:1878188/1‑148 (MQ=255)
                                                                                                        tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAAATATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCgc                                               >  2:2618962/1‑149 (MQ=255)
                                                                                                         ccaaaccaccAACATTTTAAAATCCTGTCCTTTCTTTACCATTACAAAATATAATCAAATTATTTTATTTTAAATATCCACAAAACCAACAAAAAAACGACAATATGAAAATTATACGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                              <  2:1113386/146‑1 (MQ=255)
                                                                                                           aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGTGGAGTGGAAGGCAAAAAAAACGCCACCCGATAg                                            >  2:2394702/1‑150 (MQ=255)
                                                                                                            tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATTAAAATTATTCGAGGAGTGTAAGGCAAAAAAACGGCCTCCCGATAgg                                           >  2:4106594/1‑150 (MQ=255)
                                                                                                                   tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                    <  2:2762322/150‑1 (MQ=255)
                                                                                                                    aCATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGt                                   <  2:629923/150‑1 (MQ=255)
                                                                                                                      aTTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATAAACAGATAACGATCACAAAAAACACAAAATGAAAATTATTCGAGGAGTGCAAGGCAAAAAAAAGGCCGCCCCCGAGGGAAGCCGGAg                                 >  2:4207668/1‑150 (MQ=255)
                                                                                                                       ttttCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCACCCGATAGGGAAGCCGTAGc                                >  2:1316659/1‑150 (MQ=255)
                                                                                                                          tCATATCCTGTCGTTTTTTTTCTATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  1:4491557/150‑1 (MQ=255)
                                                                                                                          tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  2:2929554/150‑1 (MQ=255)
                                                                                                                          tCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATGAAATTATTTTTTTTTAAATATCGAGATAACCATCACAAAAACGACAATATGAAAATTATTCGAGGAGGGGAAGGCAAAAAAACGGCCTCCCGATAGGGGAGCCGGAGCaaa                             >  2:2112983/1‑150 (MQ=255)
                                                                                                                            atttCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                           <  2:474542/147‑1 (MQ=255)
                                                                                                                                ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  2:1282761/150‑1 (MQ=255)
                                                                                                                                  tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     >  2:1386462/1‑150 (MQ=255)
                                                                                                                                    tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                   >  2:5410297/1‑150 (MQ=255)
                                                                                                                                    tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                   >  2:4622513/1‑150 (MQ=255)
                                                                                                                                    tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                   >  2:5410339/1‑150 (MQ=255)
                                                                                                                                      gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 >  2:4988456/1‑150 (MQ=255)
                                                                                                                                          gtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  2:1085366/150‑1 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  2:4179928/1‑150 (MQ=255)
                                                                                                                                             ttCGATTTCAAATTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          <  2:9155/150‑1 (MQ=255)
                                                                                                                                             ttCGATTTCAAAATATTATGATATTATTTGTTTTTAAATAGCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGTGGTGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          <  1:1194880/150‑1 (MQ=255)
                                                                                                                                                 aaaacAAAAAATAATGAAAATAATTGTTAAAAAAAATCGACAAAACGATCACAAAAACGACAATATGACAAATATTCGAGGAGTGAAAGCCAAAAAAACCCCCTCCCGAAAGGGACCCCGAAGCAAAGTGCTCGTGATTTTATGCCGGAt      >  2:5448650/5‑150 (MQ=255)
                                                                                                                                                 aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt      >  2:2837064/1‑150 (MQ=255)
                                                                                                                                                  tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg     <  2:4294161/150‑1 (MQ=255)
                                                                                                                                                  tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTGTTATGCCGGATg     >  2:3531293/1‑150 (MQ=255)
                                                                                                                                                  attCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCACCCGATAGGGAAGCCGTAGCACAGGGCGCGTGTTGTTATGCCGGATg     >  2:1668297/2‑150 (MQ=255)
                                                                                                                                                    tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   >  1:488313/1‑150 (MQ=255)
                                                                                                                                                     cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  2:1426401/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177085‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: