Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 12.9% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT12.9% 634.0 / 10.6 268intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (116/101);  new base T (33/13);  total (149/119)
Fisher's exact test for biased strand distribution p-value = 3.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

TCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177098‑2177394
                                                                                                                                                   |                                                                                                                                                      
tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                      <  2:3648348/150‑1 (MQ=255)
    ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                                  >  2:1839376/1‑150 (MQ=255)
    ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATaattaaatta                                                                                                                                                  >  1:4248065/1‑150 (MQ=255)
      accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                                <  1:4072526/150‑1 (MQ=255)
      accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAAAGAAattatt                                                                                                                                                >  1:1904412/1‑150 (MQ=255)
       ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                               <  2:3151863/150‑1 (MQ=255)
           ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                           <  2:3110828/150‑1 (MQ=255)
              cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                        <  2:2362512/150‑1 (MQ=255)
               aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                                       <  2:2982035/150‑1 (MQ=255)
                tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTaaa                                                                                                                                      >  2:5195627/1‑150 (MQ=255)
                   tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAatat                                                                                                                                   >  2:4462581/1‑150 (MQ=255)
                    ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAAAATGAAATTATTTTTTTTTAAatata                                                                                                                                  >  2:4371795/1‑149 (MQ=255)
                    ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAATTATTTTTTTTTAAatata                                                                                                                                  >  2:135608/1‑149 (MQ=255)
                     tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  1:2155848/150‑1 (MQ=255)
                     tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  2:409780/150‑1 (MQ=255)
                       gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                              >  2:429129/1‑150 (MQ=255)
                       gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGATTTTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTAAATATAgaa                                                                                                                               >  1:3794415/1‑149 (MQ=255)
                            tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                         <  1:4202604/150‑1 (MQ=255)
                              tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                                       <  2:4903232/150‑1 (MQ=255)
                                  tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                                   >  2:1338368/1‑150 (MQ=255)
                                  tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAAATATTTTTTTTTAAATATCGATATAAAGATcac                                                                                                                   >  1:3257732/1‑150 (MQ=255)
                                  tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATCGAGATTACGATaac                                                                                                                   >  1:3615696/1‑150 (MQ=255)
                                    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGATATAACGATCACaa                                                                                                                 >  2:2250864/1‑150 (MQ=255)
                                    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGAGTTCAAAATAAAATGAAAATATTTGTTTTTAAATATAGAGATAACGATCCCaa                                                                                                                 >  1:1085366/1‑150 (MQ=255)
                                       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              <  1:4856438/150‑1 (MQ=255)
                                       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              <  1:518722/150‑1 (MQ=255)
                                          gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                           <  2:1249863/150‑1 (MQ=255)
                                          gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTTAATATGGAGATAACGATAACAAAAACGa                                                                                                           >  1:4491771/1‑150 (MQ=255)
                                             ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAAGTAAATATTTTTTTTTTAATATAGAGATAACGATCAAAAAAAAGACaa                                                                                                        >  1:5337636/1‑150 (MQ=255)
                                              gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATTAAAATATTTTTTTTTTAATATAGATATAACGATAACAAAAAACACaaa                                                                                                       >  1:629923/1‑149 (MQ=255)
                                               aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACaaaa                                                                                                      >  1:3167885/1‑148 (MQ=255)
                                                    cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTTAATATAGAGATAAAGATAAAAAAAAACACAAAATTaaa                                                                                                 >  2:5090/1‑150 (MQ=255)
                                                    cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                                 <  2:815891/150‑1 (MQ=255)
                                                    cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTTAATATAGAGATAACGATCACAAAAACGACAATATGaaa                                                                                                 >  2:4210071/1‑150 (MQ=255)
                                                     aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAATTATTTTTTTTTAAATATAGAGGTAACGATAACAACAAAGACAATATGaaaa                                                                                                >  1:1481156/1‑150 (MQ=255)
                                                     aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGAATTAAAAATATAATTTAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAACGACAAAATGtaaa                                                                                                >  1:153695/1‑150 (MQ=255)
                                                      tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAAAATTAAAATATTTTTTTTTTAAAAACGAGATAACCATAACAAAAAAGACAAAATTAAAat                                                                                               >  1:2567540/1‑150 (MQ=255)
                                                      tAACCGCCGCGCTGTGAGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTATCAAATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATTAAATTATTTGTTTTTAAATATAGAGATTCCGATCACAAAAAAGACAGAATGAGAat                                                                                               >  2:3284295/1‑150 (MQ=255)
                                                       aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATTTGTTTTTTAATATCGAGAGAAACATCACAAAAACGACAAAATTaaaaat                                                                                              >  1:4432796/1‑148 (MQ=255)
                                                           gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                                          <  2:4150462/150‑1 (MQ=255)
                                                           gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAAGAAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAAAGACAATATTAAAattatta                                                                                          >  2:112503/1‑149 (MQ=255)
                                                           gccgcGCTGTGCGGTGTTCAGAATCTGCTTTGTCGATACCACGTACATTTACATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAAATAAAAAATTTTATTTTAAAAAAAGAGATAAACAAAACAAAAAAGAAAAAAATAAAATTAATc                                                                                          >  2:2892261/1‑150 (MQ=255)
                                                            ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAAATATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCg                                                                                         >  2:4708841/1‑150 (MQ=255)
                                                              gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAAATAAATTAATTTTTTTTAAATAAATAGATAAACAAAACAAAAAAAACAAAATTAAAAATAATagag                                                                                       >  1:1482328/1‑150 (MQ=255)
                                                              gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTAATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                                       <  2:2773441/150‑1 (MQ=255)
                                                                  tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGTATTTAAAAAAAAAAATAAAATAATTTTTTTTTAATATAGAGATAACGAAAACAAAAAAAACAAAAAGAAAAATATTAGAGGAGt                                                                                   >  1:2474491/1‑150 (MQ=255)
                                                                  tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGATAATTATTCGAGGAGt                                                                                   <  2:3439891/150‑1 (MQ=255)
                                                                     gCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                                <  2:465979/150‑1 (MQ=255)
                                                                       tttgttCAGAATCTGCTTTGTCGCTACCACGTACTTTTAAATATCCTTTCTTTTTTTTTCTATTACAAAATATAATTAAATTATTTGTTTTTAAATATCGATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                              <  1:3369918/149‑1 (MQ=255)
                                                                           ttCAGATTCTGCTTTGTCTATACCACTTAAATTTTCATCTCCTGTGTTTTTTTTTCTATTTAAAAATATAATCAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                                          <  1:1125490/150‑1 (MQ=255)
                                                                             cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                                        <  2:5488427/150‑1 (MQ=255)
                                                                               gCATCTGCTTTGTCTATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaa                                                                      <  2:1534863/150‑1 (MQ=255)
                                                                                cttCTGCTTTTTCTATACCACTTACATTTTCATATCCTGTCTTTTTTTTTCTTTTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCGAGATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                     <  1:2659829/148‑1 (MQ=255)
                                                                                cATCTGCTTTGTCAATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                     <  2:4135797/150‑1 (MQ=255)
                                                                                 aTCTGCTTTGTCTATACCACGTACATTTTAATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATGAAATTTTTTTTTTTTAAATATCTAGATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGCAGTGAAAGGCAAAAAAAc                                                                    <  1:429129/150‑1 (MQ=255)
                                                                                 aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGATTTTAAAAAATAAATTAAATATTTTTTTTTTTAAAAAGACATAAACATGAAAAAAAAAAACAAATGTAAAATATTAGAGGATTGTAATGCcaaaaaaa                                                                    >  1:2613865/1‑149 (MQ=255)
                                                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAAAATAAATAAAAAATTTGTTTTTAAAAAAAGATAAAAAAATAACAAAAAAAACAAAAAGAAAAAAATTAGAGGGGTGATAGTGaaaaaaaaa                                                                   >  1:4966020/1‑148 (MQ=255)
                                                                                    tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGc                                                                 >  2:1641189/1‑150 (MQ=255)
                                                                                    tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTTAAAAAATAAATAAAATATTTTTTTTTTAATATAGAGATAAAGAAAAAAAAAAAAAAAAAAAGAAAAATATTAGAGGGGGGTAAGGCaaaaaaaaagc                                                                 >  1:2374895/1‑146 (MQ=255)
                                                                                       tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGGTTTAAAAAAATAAATAAAATAATTTTTTTTAAATATAGATGTAAAGAAAACAAAAAAGACAAAATGTAAATTATTATGGGAGTGTAAGGCaaaaaaaacgccac                                                              >  1:378168/1‑143 (MQ=255)
                                                                                       tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTCGATTTCAAAATATAATTAAAATATTTTTTTTTAAATATCCCGATAACGATCACCCCAACGACCAACTGCAAAATATTCGCGGCGGGCCAGGCCAAAAAACGGcccc                                                              >  2:1127154/1‑148 (MQ=255)
                                                                                           tCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAAAATAATGAAAATATTTTTTTTTTAATTTAGATGTTAACATCACAAAAAACACAATATGAAAATTTTTTGAGGTGTGTAAGGCCAAAAAAACGCCAccccg                                                          >  2:1878188/1‑148 (MQ=255)
                                                                                           tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAAATATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCgc                                                          >  2:2618962/1‑149 (MQ=255)
                                                                                            cGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                                         <  2:1303703/150‑1 (MQ=255)
                                                                                              aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGTGGAGTGGAAGGCAAAAAAAACGCCACCCGATAg                                                       >  2:2394702/1‑150 (MQ=255)
                                                                                               tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                      <  2:2122947/150‑1 (MQ=255)
                                                                                                aCCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGCTAggg                                                     >  2:1478351/1‑150 (MQ=255)
                                                                                                     gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                <  2:4080689/150‑1 (MQ=255)
                                                                                                       aCATTTTAATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGt                                              <  2:629964/150‑1 (MQ=255)
                                                                                                        cATTTTCATATCCTTTCGTTTTTTTTCTATTTCAAAATATATTTAATTTATTTGTTTTTAAATTTCGTGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  1:3440554/150‑1 (MQ=255)
                                                                                                        cATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  2:1073556/150‑1 (MQ=255)
                                                                                                         aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGATATAAAGATAAAAAAAAAGAAAAAATGAAAATTATTAGAGGATTGAAATGCAAAAAAACGGCCTACCGATAGGGAAGCCGTAg                                            >  2:5296158/1‑150 (MQ=255)
                                                                                                         aTTTTCATATCCTGGCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                            <  2:3843467/150‑1 (MQ=255)
                                                                                                                       tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                              >  2:5410297/1‑150 (MQ=255)
                                                                                                                            tgtttTCGATTTCAAAATATACTGAATTTTTTTGTTTTTAATTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                         <  1:1897959/150‑1 (MQ=255)
                                                                                                                             gtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        <  2:2525301/150‑1 (MQ=255)
                                                                                                                               tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAATTCGACAATATGAAAATTTTTCGAGGAGTGAATGGGTATATTTCGGCCTCGCGTTTGGGAAGCGGTAGCTTAGTGCGGGTGTTTTTTTgg                      >  2:5424008/1‑149 (MQ=255)
                                                                                                                               ataccaTTTCAAAAAATAATTAAATTATTTGTTATTAAATATCTAGATAACGAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                      <  2:110464/145‑1 (MQ=255)
                                                                                                                                ttCGATTTCAAATTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     <  2:9155/150‑1 (MQ=255)
                                                                                                                                ttCGATTTCAAAATATTATGATATTATTTGTTTTTAAATAGCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGTGGTGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     <  1:1194880/150‑1 (MQ=255)
                                                                                                                                ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:4197643/1‑150 (MQ=255)
                                                                                                                                ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCTGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:4734590/1‑150 (MQ=255)
                                                                                                                                ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:4758534/1‑150 (MQ=255)
                                                                                                                                   gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAATCGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                  >  2:742857/1‑150 (MQ=255)
                                                                                                                                   gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                  >  1:651347/1‑150 (MQ=255)
                                                                                                                                    aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGTTTTTATGCCGGAt                 >  1:815735/1‑150 (MQ=255)
                                                                                                                                    aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                 >  2:2837064/1‑150 (MQ=255)
                                                                                                                                     tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTGTTATGCCGGATg                >  2:3531293/1‑150 (MQ=255)
                                                                                                                                     attCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCACCCGATAGGGAAGCCGTAGCACAGGGCGCGTGTTGTTATGCCGGATg                >  2:1668297/2‑150 (MQ=255)
                                                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              <  1:1755801/150‑1 (MQ=255)
                                                                                                                                        cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             >  2:1426401/1‑150 (MQ=255)
                                                                                                                                        aaaaaTATAATTAAATTATTTGTTTTTAAATATCTAGATACCGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             <  1:1853512/149‑1 (MQ=255)
                                                                                                                                           aaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATCTGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt          <  2:533775/150‑1 (MQ=255)
                                                                                                                                             tttaATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTTTGCCGGATGCGGTGTaa        <  1:4181786/148‑1 (MQ=255)
                                                                                                                                             tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        <  1:4462581/150‑1 (MQ=255)
                                                                                                                                             tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        <  2:1904412/150‑1 (MQ=255)
                                                                                                                                              ataCTGAAATTATTTGTTTTTAAATATCGAGATAACGAGCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       <  2:5003096/150‑1 (MQ=255)
                                                                                                                                                  tGAAATTATTTGTTTTTAAATATCGAGATAACGATCTCAAAAACGAGAATATGTAATTTATTCGAGGTGTGATAGGGAAAATATCGGGCTCGCGTTTGGGAAGCGGTAGCTTAGTGGGGGTGTTTTTGTGCGGGATGGGgtgttatgatt   >  2:3415338/1‑143 (MQ=255)
                                                                                                                                                   gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  >  1:4676734/1‑150 (MQ=255)
                                                                                                                                                   gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  >  1:2880910/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
TCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177098‑2177394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: