Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,282,833 G→A 5.2% intergenic (+32/+107) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,8330GA 5.2% 1122.1 / 27.3 325intergenic (+32/+107)rplY/yejK50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (175/133);  new base A (12/5);  total (187/138)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 338 total aligned reads displayed.

CTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAA  >  NC_000913/2282684‑2282982
                                                                                                                                                     |                                                                                                                                                       
cTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg                                                                                                                                                         >  2:3898070/1‑150 (MQ=255)
 tACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcgg                                                                                                                                                        >  2:2194308/1‑150 (MQ=255)
      cGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCgggg                                                                                                                                                   >  2:4285483/1‑150 (MQ=255)
       gAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAATCCCCGCTCCGGCGGGGt                                                                                                                                                  >  2:2957268/1‑150 (MQ=255)
       gAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTACAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGt                                                                                                                                                  <  1:4118883/150‑1 (MQ=255)
        aaGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtt                                                                                                                                                 <  1:4890152/150‑1 (MQ=255)
           ttCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGttttt                                                                                                                                              <  2:3406984/150‑1 (MQ=255)
            tCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtttttt                                                                                                                                             <  2:4753870/150‑1 (MQ=255)
                aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTAt                                                                                                                                         >  2:2311719/1‑150 (MQ=255)
                aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTAt                                                                                                                                         >  2:4216649/1‑150 (MQ=255)
                aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTAt                                                                                                                                         >  2:2677126/1‑150 (MQ=255)
                 ccATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATc                                                                                                                                        >  2:2479398/1‑150 (MQ=255)
                     cGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCa                                                                                                                                    >  2:4907612/1‑150 (MQ=255)
                        tgttgACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGAt                                                                                                                                 >  2:829771/1‑150 (MQ=255)
                          ttgACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTa                                                                                                                               >  1:3680092/1‑150 (MQ=255)
                          ttgACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTa                                                                                                                               >  2:548271/1‑150 (MQ=255)
                          ttgACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTa                                                                                                                               >  2:1974389/1‑150 (MQ=255)
                             aCGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTACAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGc                                                                                                                            <  1:319082/150‑1 (MQ=255)
                                     gAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTAc                                                                                                                    >  1:101519/1‑150 (MQ=255)
                                     gAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTAc                                                                                                                    >  1:3431679/1‑150 (MQ=255)
                                     gAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTAc                                                                                                                    >  1:2541664/1‑150 (MQ=255)
                                       aaTCAAAGTTAACGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACgg                                                                                                                  <  1:723729/150‑1 (MQ=255)
                                       aaTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACgg                                                                                                                  <  1:2900038/150‑1 (MQ=255)
                                         tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTa                                                                                                                >  1:962622/1‑150 (MQ=255)
                                         tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTa                                                                                                                <  1:76974/150‑1 (MQ=255)
                                         tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTa                                                                                                                >  2:1458891/1‑150 (MQ=255)
                                         tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTa                                                                                                                <  1:1896658/150‑1 (MQ=255)
                                           aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAtt                                                                                                              >  2:895188/1‑150 (MQ=255)
                                           aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAtt                                                                                                              <  1:1530415/150‑1 (MQ=255)
                                                     cTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCTGATTATGCCTGATTACGGTATTGCTAtttttt                                                                                                    >  2:3664594/1‑150 (MQ=255)
                                                     cTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt                                                                                                    <  2:2357205/150‑1 (MQ=255)
                                                     cTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt                                                                                                    >  1:3841415/1‑150 (MQ=255)
                                                      tCAGGACGTACAGCGTCACCCGTACAACCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTg                                                                                                   <  2:1115998/150‑1 (MQ=255)
                                                      tCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTg                                                                                                   <  2:2782405/150‑1 (MQ=255)
                                                        aGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGACTAAGTTGTAAACAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGcg                                                                                                 >  1:3540561/1‑149 (MQ=255)
                                                         ggACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAg                                                                                                <  1:2194343/150‑1 (MQ=255)
                                                              tACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCGGACTAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTCTCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAg                                                                                           >  2:2995743/1‑150 (MQ=255)
                                                               aCAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATGTTTTGCAGGCCAGa                                                                                          >  2:3205645/1‑150 (MQ=255)
                                                                  gCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATaa                                                                                       <  2:5149824/150‑1 (MQ=255)
                                                                  gCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATaa                                                                                       <  2:577694/150‑1 (MQ=255)
                                                                  gCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATaa                                                                                       <  2:1188066/150‑1 (MQ=255)
                                                                       aCCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGt                                                                                  <  1:3050429/150‑1 (MQ=255)
                                                                         ccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTc                                                                                <  1:551833/150‑1 (MQ=255)
                                                                         ccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCACGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGTTATTGCTATTTTTTGCATGCCAGATAAGGCTTTc                                                                                >  2:4440096/1‑150 (MQ=255)
                                                                          cGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCa                                                                               <  1:1387986/150‑1 (MQ=255)
                                                                              cAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgcc                                                                           <  1:5042473/150‑1 (MQ=255)
                                                                              cAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgcc                                                                           <  1:443325/150‑1 (MQ=255)
                                                                                aaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgccgc                                                                         <  1:4809293/150‑1 (MQ=255)
                                                                                  ccGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCAt                                                                       >  2:921007/1‑150 (MQ=255)
                                                                                  ccGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCAt                                                                       >  1:1814502/1‑150 (MQ=255)
                                                                                   cGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATc                                                                      <  1:5218837/150‑1 (MQ=255)
                                                                                    gAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCt                                                                     <  2:204642/150‑1 (MQ=255)
                                                                                     aaGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTg                                                                    <  1:1220694/150‑1 (MQ=255)
                                                                                      aGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTgg                                                                   <  2:4721674/150‑1 (MQ=255)
                                                                                        cTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCa                                                                 <  1:2087713/150‑1 (MQ=255)
                                                                                        cTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAATTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCa                                                                 <  2:214687/150‑1 (MQ=255)
                                                                                          gcagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCAtt                                                               <  2:1687440/150‑1 (MQ=255)
                                                                                           cagcaCATCGACTGCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCTGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTc                                                              >  2:1066125/1‑150 (MQ=255)
                                                                                               ccaTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa                                                          <  1:5450924/149‑1 (MQ=255)
                                                                                               acaTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa                                                          <  2:4070807/150‑1 (MQ=255)
                                                                                                 aTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAaca                                                        >  1:1436059/1‑150 (MQ=255)
                                                                                                  tCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAacac                                                       >  1:4718781/1‑150 (MQ=255)
                                                                                                    gACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaa                                                     <  2:4806922/150‑1 (MQ=255)
                                                                                                    gACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaa                                                     >  2:4513460/1‑150 (MQ=255)
                                                                                                    gACTTCGTTCGCGCTTAATTACTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaa                                                     >  1:2812279/1‑150 (MQ=255)
                                                                                                        tcgttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAAcc                                                 <  1:5284848/150‑1 (MQ=255)
                                                                                                         cgttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCg                                                >  1:2573426/1‑150 (MQ=255)
                                                                                                            tcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTCTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGAtt                                             >  2:1893856/1‑150 (MQ=255)
                                                                                                              gcgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTaa                                           >  2:1494780/1‑150 (MQ=255)
                                                                                                              gcgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGAAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTaa                                           >  1:1857662/1‑150 (MQ=255)
                                                                                                                 cTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTg                                        >  2:5095823/1‑150 (MQ=255)
                                                                                                                    aaTTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgc‑‑cg                                   <  2:2828772/150‑1 (MQ=255)
                                                                                                                     aTTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgc‑‑cgc                                  <  2:4316687/150‑1 (MQ=255)
                                                                                                                     aTTGCTGAATAAGGTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgc‑‑cgc                                  <  1:3990777/150‑1 (MQ=255)
                                                                                                                       tGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCa                                <  2:1861242/150‑1 (MQ=255)
                                                                                                                         cTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTAAGAACACAAAACCGATTAATTGC‑‑CGCCAGa                              >  1:4319696/1‑150 (MQ=255)
                                                                                                                          tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGAAGATTATGGCTGATTAAGGTATTGCTATTTTTTTCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGAc                             >  1:2317712/1‑150 (MQ=255)
                                                                                                                           gAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACg                            >  2:622090/1‑150 (MQ=255)
                                                                                                                            aaTAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGt                           >  2:3488159/1‑150 (MQ=255)
                                                                                                                            aaTAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCCATTAATTTC‑‑CGCCCGACGt                           >  1:1086151/1‑150 (MQ=255)
                                                                                                                             aTAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATATGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCCCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCCGACGTc                          >  1:3718222/1‑150 (MQ=255)
                                                                                                                               aaGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCg                        <  1:4519221/150‑1 (MQ=255)
                                                                                                                                 gTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGAAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGgc                      >  2:5258107/1‑150 (MQ=255)
                                                                                                                                  ttGTAAAAAACCACGCTCCGGCGGGGTTTTTTGTATCAGGAGATTATGCCTGATTAAGTTATTGCAATTTTTTGCAGGCCAGATAAGGCGTACACGCCGCATCTGGCATGAAGTACACCAAACCGATTAAATGA‑‑CGCCAGACGTCCGgcg                     >  1:3284361/1‑150 (MQ=255)
                                                                                                                                    gTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCt                   <  2:1277858/150‑1 (MQ=255)
                                                                                                                                    gTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCt                   >  1:3894253/1‑150 (MQ=255)
                                                                                                                                     tAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCTg                  >  2:4242576/1‑150 (MQ=255)
                                                                                                                                       aaaaaCCCCGCCGGAGCGGGGGTTTTTGTATCAGCGGATTATGCCTGATTACGGGATTGCTATTTTTTGCAGGCCAGATAAGTCGTTCACGCCGCCTCTGGCAATCAGAAAACAAAACCCATTAATTGC‑‑CGCCAGACCTCCGGCGCGGcc                >  1:4147082/1‑149 (MQ=255)
                                                                                                                                        aaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCTGCaa               >  2:3553236/1‑150 (MQ=255)
                                                                                                                                        aaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGAGAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCTGCaa               >  2:4635876/1‑150 (MQ=255)
                                                                                                                                         aaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCAGCGCCGGACGTCTGgcggcaat                <  1:2261739/150‑6 (MQ=255)
                                                                                                                                         aaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGAGTATGCCGGATTACGGGCTTGCTATTTTTTGCAGGCCCGATAAGGCGGTCACGCCGCATCTGGCATTCAGAACACACAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCGGCAAt              >  1:4084445/1‑150 (MQ=255)
                                                                                                                                          aaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCTGCAAtt             >  2:3252949/1‑150 (MQ=255)
                                                                                                                                           aCCCTGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCTGCAATTg            >  1:4909023/1‑150 (MQ=255)
                                                                                                                                              ccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTTCAGGCCCGAAAAGGCGTTCACGCCCCAACTGGCAATAAGAACACAAAACCGATTAATTTC‑‑CGCCAGACGTCCGGCGCTGCAATTGGTc         >  1:1747723/1‑150 (MQ=255)
                                                                                                                                              ccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAATGCGTTCACGCCGCATCTGGAATTCAGAACACAAAAAAGATTAATAGC‑‑CGCCAGACGTCCGGCGCTGCAATTGGTc         >  2:3065663/1‑150 (MQ=255)
                                                                                                                                              ccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCTGCAATTGGTc         >  2:2536044/1‑150 (MQ=255)
                                                                                                                                              ccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCTGCAATTGGTc         >  1:5314988/1‑150 (MQ=255)
                                                                                                                                               cGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCTGCAATTGGTcg        >  1:3132516/1‑150 (MQ=255)
                                                                                                                                                     ggcggGGTTTTTTTTATAATCAGATTATGACAGAATAAGGTATTGCTATTTTTTTCATGCCAGATAAGGCGTTCACGCCCCATAAGGCATTAAGAAAACAAAAACAATTAAATTC‑‑AGCCCGAAGTCCGGCGCTGCAATAGGTCGCGCaaa  >  1:5400083/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
CTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGC‑‑CGCCAGACGTCCGGCGCTGCAATTGGTCGCGCAAA  >  NC_000913/2282684‑2282982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: