Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,409,191 A→C 5.7% E110D (GAA→GAC)  yfbR → 5'‑nucleotidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,409,1910AC 5.7% 988.9 / 5.8 317E110D (GAA→GAC) yfbR5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base A (153/139);  new base C (22/3);  total (175/142)
Fisher's exact test for biased strand distribution p-value = 5.39e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

ATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCA  >  NC_000913/2409042‑2409338
                                                                                                                                                     |                                                                                                                                                   
   gCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAagag                                                                                                                                                  >  1:815669/1‑150 (MQ=255)
   gCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAagag                                                                                                                                                  >  1:3583159/1‑150 (MQ=255)
    cTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGACGAGc                                                                                                                                                 >  2:1080484/1‑150 (MQ=255)
    cTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGc                                                                                                                                                 >  2:3819256/1‑150 (MQ=255)
     tttACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATCTGGTTCCGGACGAGCt                                                                                                                                                >  1:4780389/1‑150 (MQ=255)
      ttACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTg                                                                                                                                               >  2:2042554/1‑150 (MQ=255)
       tACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGc                                                                                                                                              <  1:1039280/150‑1 (MQ=255)
          tGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCggg                                                                                                                                           >  1:4212818/1‑150 (MQ=255)
           ggCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGa                                                                                                                                          <  1:3326066/150‑1 (MQ=255)
            gCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGat                                                                                                                                         <  2:4987915/150‑1 (MQ=255)
              gATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGatat                                                                                                                                       >  1:5450048/1‑150 (MQ=255)
                tGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCt                                                                                                                                     <  1:4337853/150‑1 (MQ=255)
                    ccACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGAGATATCTATgc                                                                                                                                 >  2:938832/1‑150 (MQ=255)
                     cACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTgcg                                                                                                                                >  2:3096189/1‑150 (MQ=255)
                              tCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTTAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAAtt                                                                                                                       <  1:5413811/150‑1 (MQ=255)
                              tCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACGAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAAtt                                                                                                                       <  1:2235091/150‑1 (MQ=255)
                              tCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGACGAGCTGCGGGATATCTTTGCGCCGTTAAtt                                                                                                                       >  1:3510214/1‑150 (MQ=255)
                              tCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAAtt                                                                                                                       >  2:2220894/1‑150 (MQ=255)
                              tCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAAtt                                                                                                                       <  1:2360097/150‑1 (MQ=255)
                              tCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATAGCTTTGCGCCGTTAAtt                                                                                                                       >  2:3250287/1‑150 (MQ=255)
                                aGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGa                                                                                                                     >  2:3608523/1‑150 (MQ=255)
                                 gAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGAc                                                                                                                    <  1:1243427/150‑1 (MQ=255)
                                 gAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGAc                                                                                                                    >  1:2520961/1‑150 (MQ=255)
                                       cTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcat                                                                                                              <  1:1703081/150‑1 (MQ=255)
                                       cTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcat                                                                                                              >  2:1264493/1‑150 (MQ=255)
                                        tCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcatg                                                                                                             >  1:2408529/1‑150 (MQ=255)
                                        tCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcatg                                                                                                             <  1:828771/150‑1 (MQ=255)
                                        tCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcatg                                                                                                             >  1:2015770/1‑150 (MQ=255)
                                         cACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcatgc                                                                                                            >  1:3761116/1‑150 (MQ=255)
                                         cACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcatgc                                                                                                            <  1:2672119/150‑1 (MQ=255)
                                           ccGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGACGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAgcatgcat                                                                                                          >  1:2773389/1‑150 (MQ=255)
                                            cGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCata                                                                                                         >  1:5038386/1‑150 (MQ=255)
                                            cGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCata                                                                                                         >  1:1500882/1‑150 (MQ=255)
                                              gCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGACGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCAtata                                                                                                       >  1:4562207/1‑150 (MQ=255)
                                                gATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGc                                                                                                     <  1:4909545/150‑1 (MQ=255)
                                                   ctcCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATGGCGAt                                                                                                  <  2:2762204/150‑1 (MQ=255)
                                                   ctcCCTACTCCCGTGAAAAACTTCAATTCCCAAATCGCACAGGAGTACAACGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGAt                                                                                                  <  2:4367014/150‑1 (MQ=255)
                                                      ccTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATgaa                                                                                               <  1:2185308/150‑1 (MQ=255)
                                                      ccTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATgaa                                                                                               <  1:4175319/150‑1 (MQ=255)
                                                      ccTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATgaa                                                                                               <  2:1410607/150‑1 (MQ=255)
                                                       cTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATgaag                                                                                              <  1:5120981/150‑1 (MQ=255)
                                                          cTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGaaa                                                                                           <  2:2571866/150‑1 (MQ=255)
                                                             cGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGaaaatc                                                                                        <  1:4511674/150‑3 (MQ=255)
                                                             cGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAAt                                                                                        >  1:407170/1‑150 (MQ=255)
                                                              ggTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGTAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATc                                                                                       <  2:4041942/150‑1 (MQ=255)
                                                               gTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATTCATATAGCGATGAAGAAAAATCg                                                                                      <  2:5104691/150‑1 (MQ=255)
                                                               gTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCg                                                                                      <  1:2313288/150‑1 (MQ=255)
                                                                tGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGACGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGc                                                                                     >  1:4990164/1‑150 (MQ=255)
                                                                tGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGc                                                                                     >  2:3033761/1‑150 (MQ=255)
                                                                tGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGc                                                                                     <  1:859387/150‑1 (MQ=255)
                                                                     tACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTg                                                                                >  2:2476849/1‑150 (MQ=255)
                                                                        ttCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGaaa                                                                             >  2:2376302/1‑150 (MQ=255)
                                                                          cAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACa                                                                           >  1:2138561/1‑150 (MQ=255)
                                                                              tCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCa                                                                       <  2:4926479/150‑1 (MQ=255)
                                                                              tCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCa                                                                       <  2:1818649/150‑1 (MQ=255)
                                                                                 cAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGAt                                                                    >  2:5439812/1‑150 (MQ=255)
                                                                                    aTCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCa                                                                 >  2:431990/1‑150 (MQ=255)
                                                                                    aTCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCa                                                                 <  2:1658059/150‑1 (MQ=255)
                                                                                    aTCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGGTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCa                                                                 >  2:1462642/1‑150 (MQ=255)
                                                                                        cTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACtgt                                                             <  1:1454809/150‑1 (MQ=255)
                                                                                        cTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACtgt                                                             >  1:2867199/1‑150 (MQ=255)
                                                                                         tCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACtgtg                                                            >  1:336969/1‑150 (MQ=255)
                                                                                         tCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACtgtg                                                            <  2:167962/150‑1 (MQ=255)
                                                                                          cAGGAGTACACGGCTATTTAAAAAATCGCACACCAAAAACTGGTCGATATGGGTCCGGACGTGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCTTGCATATAGGGATTAAGAAAAATCGCTGGTGAAACAGGCAGATGCACtgtgt                                                           <  1:2473832/150‑1 (MQ=255)
                                                                                             gAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGACGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCa                                                        >  1:4465966/1‑150 (MQ=255)
                                                                                                   aaGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTg                                                  <  1:5282678/150‑1 (MQ=255)
                                                                                                    aGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGa                                                 <  1:3246993/150‑1 (MQ=255)
                                                                                                       cTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAAt                                              >  2:26752/1‑150 (MQ=255)
                                                                                                          ttGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTc                                           >  2:2330549/1‑150 (MQ=255)
                                                                                                           tGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCt                                          >  1:4819576/1‑150 (MQ=255)
                                                                                                             aaaaaaTCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTgg                                        >  1:193689/1‑150 (MQ=255)
                                                                                                               aaaaTCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGgaa                                      <  1:2541009/150‑1 (MQ=255)
                                                                                                                      cTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCCGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTcg                               >  1:1582743/1‑150 (MQ=255)
                                                                                                                       tCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTcgc                              >  2:105313/1‑150 (MQ=255)
                                                                                                                       tCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTcgc                              >  2:5362015/1‑150 (MQ=255)
                                                                                                                       tCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTcgc                              >  1:3861172/1‑150 (MQ=255)
                                                                                                                           cAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGcc                          >  2:3975686/1‑150 (MQ=255)
                                                                                                                                  tGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAaata                   >  2:2838010/1‑150 (MQ=255)
                                                                                                                                  tGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAaata                   >  2:3026374/1‑150 (MQ=255)
                                                                                                                                  tGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAaata                   >  2:3921763/1‑150 (MQ=255)
                                                                                                                                  gggTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAaata                   <  1:4661989/149‑1 (MQ=255)
                                                                                                                                   ggTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATTAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAactaa                  >  1:720690/1‑150 (MQ=255)
                                                                                                                                   ggTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAaataa                  <  1:3608523/150‑1 (MQ=255)
                                                                                                                                     tCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATg                <  2:4257693/150‑1 (MQ=255)
                                                                                                                                        atatGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAAt             >  2:475266/1‑150 (MQ=255)
                                                                                                                                        atatGGTTCCGCAAGAGCTGCGGTATAACTTTCCGCCCTTAATTGACGACCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGAATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAAt             <  1:994564/150‑1 (MQ=255)
                                                                                                                                          atGGTTCCGGACGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTc           >  1:4274250/1‑150 (MQ=255)
                                                                                                                                          atGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTc           >  1:3044352/1‑150 (MQ=255)
                                                                                                                                             gTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATCTCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTg        >  1:1262200/1‑150 (MQ=255)
                                                                                                                                             gTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGTAGAACTCGCGGCCGGAAATAATGAATTCTTg        <  2:3745712/150‑1 (MQ=255)
                                                                                                                                             gTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTg        <  2:3478399/150‑1 (MQ=255)
                                                                                                                                             gTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTg        >  2:518235/1‑150 (MQ=255)
                                                                                                                                             gTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTg        <  2:4861245/150‑1 (MQ=255)
                                                                                                                                              ttCCTGAAGTGCTGCGGGATTTCTTTGAGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGc       <  2:4670407/150‑1 (MQ=255)
                                                                                                                                              ttCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGc       <  1:1823634/150‑1 (MQ=255)
                                                                                                                                              ttCCGGAAGAGCTGCGGGATATCTTTGCGCCGTCAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGc       <  2:815669/150‑1 (MQ=255)
                                                                                                                                               tCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCt      <  2:620791/150‑1 (MQ=255)
                                                                                                                                                 cGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTgg    >  2:460593/1‑150 (MQ=255)
                                                                                                                                                 cGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTgg    <  1:1168566/150‑1 (MQ=255)
                                                                                                                                                   caaGAGCAGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCAGATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAAAGAATTCTTGATGGCa  >  2:3140170/2‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATCGCTTTACTGGCGATGTACCACGATGCCTCAGAAGTGCTCACCGGCGATCTCCCTACTCCGGTGAAATACTTCAATTCGCAAATCGCTCAGGAGTACAAGGCTATTGAAAAAATCGCTCAGCAAAAACTGGTCGATATGGTTCCGGAAGAGCTGCGGGATATCTTTGCGCCGTTAATTGACGAGCATGCATATAGCGATGAAGAAAAATCGCTGGTGAAACAGGCAGATGCACTGTGTGCATATCTGAAATGTCTGGAAGAACTCGCGGCCGGAAATAATGAATTCTTGCTGGCA  >  NC_000913/2409042‑2409338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑