Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,431 G→T 12.0% R434I (AGA→ATA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4310GT12.0% 468.1 / 8.2 177R434I (AGA→ATA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base G (70/78);  new base T (18/4);  total (93/84)
Fisher's exact test for biased strand distribution p-value = 2.67e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

GTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470283‑2470580
                                                                                                                                                    |                                                                                                                                                     
     tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAaataaatcaaataaatca                                                                                                                                                 >  2:4325898/1‑150 (MQ=255)
       tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAtaaaaaaaataaaaaaaaaaaaa                                                                                                                                               >  2:5201951/1‑145 (MQ=255)
        ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAACAAGa                                                                                                                                              >  2:249755/1‑150 (MQ=255)
           tGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACTGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACaa                                                                                                                                           <  1:4228178/150‑1 (MQ=255)
            ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACaaa                                                                                                                                          >  1:3148539/1‑149 (MQ=255)
            ggAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAATCaataaaaa                                                                                                                                          >  1:445835/1‑144 (MQ=255)
             gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                         <  2:1752961/150‑1 (MQ=255)
                   ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAAGAAAAAAGCGAtt                                                                                                                                   >  1:3844648/1‑149 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTAAAATAATTAAAAAAAAAAAATAAAATAACGATGt                                                                                                                                  >  1:2008092/1‑150 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGt                                                                                                                                  >  2:3227611/1‑150 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGt                                                                                                                                  >  1:3046208/1‑150 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGt                                                                                                                                  >  2:4998066/1‑150 (MQ=255)
                    tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAATAAAAACAATAGAGATGt                                                                                                                                  >  1:1522820/1‑150 (MQ=255)
                     cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTGAAATAAAAAAAGACAAAAGCGATGTa                                                                                                                                 >  1:1126121/1‑150 (MQ=255)
                     cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAAAAAGACAAAAACGATTTa                                                                                                                                 >  1:2779421/1‑150 (MQ=255)
                      aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAATAGCGATTTaa                                                                                                                                >  2:3029614/1‑150 (MQ=255)
                       aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                               <  1:3713610/150‑1 (MQ=255)
                       aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                               <  1:1973674/150‑1 (MQ=255)
                              gtgtCATTTTTCGGGGAAGGATTTAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                        <  2:1957055/150‑1 (MQ=255)
                              gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                        <  2:2153365/150‑1 (MQ=255)
                               tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAACAATAAAATAGCGATGTAAAGCACAAAg                                                                                                                       >  2:1455834/1‑150 (MQ=255)
                                gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAATTCAAAGAAATCAAGAAAATAGCGATGTAAAGAACTAAGt                                                                                                                      >  2:2200642/1‑150 (MQ=255)
                                  cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                                                                                                    <  1:1464902/150‑1 (MQ=255)
                                  cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                                                                                                    <  1:4668377/150‑1 (MQ=255)
                                  cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                                                                                                    <  1:2288017/150‑1 (MQ=255)
                                   aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAATAAAGAAAAAATCGATGTAAAGAACGAAGTTTa                                                                                                                   >  1:1907145/1‑150 (MQ=255)
                                    tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATATTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                  <  2:1579596/150‑1 (MQ=255)
                                      tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAAACAAGAAAAAAGCGATGTAAATCCAAAATTTTAAAt                                                                                                                >  1:617684/1‑150 (MQ=255)
                                      tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAATAAATCAAAAAAAAAGCGATGTAAAGCACAAAATTTAAAt                                                                                                                >  1:5314975/1‑150 (MQ=255)
                                            ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                                                                                                          <  1:4783552/150‑1 (MQ=255)
                                             gcaGGATATAGAGATTTAAGCAAGCATTGATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATCGCGCTGTAAAGCACTAAGTTTAACTTgcgcgc                                                                                                         <  2:4034881/148‑1 (MQ=255)
                                             gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAAAAAAAAACGATGTAAAAAACAAAGTTTAAATTtcgcgc                                                                                                         >  1:1314139/1‑150 (MQ=255)
                                                 gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                                                                                                     <  2:3988429/150‑1 (MQ=255)
                                                 gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATATCGATGTAAAGCAATAAGTTTAAATTGAGCGCCaaa                                                                                                     >  2:2726701/1‑149 (MQ=255)
                                                 gATATAGAGATATAAGCAAGCATATATATGGAATGTATTTTTCGTTAGACCTTTGCTTATACATAACAGTCGTTTTTTTAATATAAAAAATAAATAAAAAAAATAAAGACAATAGCTAATTAAAAAACTAAGTTTAAATTTAGAGCCaaa                                                                                                     >  2:2726613/1‑149 (MQ=255)
                                                    ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGAGCGCCAATCAt                                                                                                  >  2:394749/1‑150 (MQ=255)
                                                    ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                                                                                                  >  2:394381/1‑150 (MQ=255)
                                                     taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                 <  1:857325/150‑1 (MQ=255)
                                                     taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                 <  2:3234085/150‑1 (MQ=255)
                                                          aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcg                                                                                            <  2:832669/150‑1 (MQ=255)
                                                            ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca                                                                                          <  2:2473049/150‑1 (MQ=255)
                                                              aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATATCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGcaca                                                                                        >  2:4376346/1‑150 (MQ=255)
                                                               agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                       <  2:2831837/150‑1 (MQ=255)
                                                                 caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATTAAAAAATTAAAAGAAATAAATAAAAAATCGATGTAAAACACTAATTTTAAATTTCGCGCCAAAACAGGCGCGCACaaca                                                                                     >  1:4096576/1‑148 (MQ=255)
                                                                 caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                     <  2:1653697/150‑1 (MQ=255)
                                                                 caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAATAGCGATGTAAATCACGAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGc                                                                                     >  2:771353/1‑150 (MQ=255)
                                                                  aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATATCGATGTAAAGCAATAAGTTTAAATTGCGCGCCAAACAAGGCGCGCACAAGCt                                                                                    >  2:4055729/1‑150 (MQ=255)
                                                                  aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTTTTTTTTAAATAATTAAAATAAAAAAAAACAAAAACGATGTAAATAACAAACTTTTAATTTGGATCCACACAGGGCGCGcaccacca                                                                                    >  1:1865486/1‑145 (MQ=255)
                                                                       tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTAAAAAAATTAAAATAAAAAAAAACAAAATCGATGTAAAGCACTAAGTTTATATTTAGCGCCAAACATGGCGCGCACAAGCGataaa                                                                               >  1:303913/1‑149 (MQ=255)
                                                                       tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTataat                                                                               >  2:3657686/1‑150 (MQ=255)
                                                                        ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataata                                                                              <  2:3472989/150‑1 (MQ=255)
                                                                        ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataata                                                                              <  2:4942076/150‑1 (MQ=255)
                                                                         tttttGTAATGTATTTTTCGTTCGACCTTTGATTATAAATAACTGTCTTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGAAAATAGCTATGTAATGCAATATGTTTAATTTGCGCGCCAATCATGGCGCGCACAAGCTATAATAc                                                                             <  1:818748/148‑1 (MQ=255)
                                                                            ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCaa                                                                          <  2:2628852/150‑1 (MQ=255)
                                                                                      tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAAAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATTGCGCGCACAAGCTATAATACCAACCAAAATtct                                                                >  2:5407067/1‑150 (MQ=255)
                                                                                      tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAATCGATGTAAAGCACTAAGTTTAAATTTAGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAtttat                                                                >  2:4943488/1‑148 (MQ=255)
                                                                                       ttttCGTTCGACCTTTGCTTATACATAACATTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                               <  2:3712662/150‑1 (MQ=255)
                                                                                           cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCAATAAGTTTAAATTGCGCGCCAATCATTGCGCGCACAAGCTATAATAACAACCTAATTTctcctcc                                                           >  2:850051/1‑150 (MQ=255)
                                                                                             ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGAAAAAAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATAAAAACCTAATTTatactccgc                                                         >  2:2379107/1‑148 (MQ=255)
                                                                                                  cattttCTTTTTCATAACTGTCGTTTTTTTATTTTATAAAATAATTCTAAGAAATCAAGACAATAGCTATTTAAATAACTAATTTTAATTTGCGCGCCAATATTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                    <  1:2176165/148‑1 (MQ=255)
                                                                                                   cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTagag                                                   <  1:254133/150‑1 (MQ=255)
                                                                                                   cTTTGATTATACATAACATTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTagag                                                   <  1:253995/150‑1 (MQ=255)
                                                                                                    tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCAAATTTCTCCTCCTCTTAGAGt                                                  >  2:3934842/1‑150 (MQ=255)
                                                                                                    tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATATCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAAAACCAAACTAATTTCTCCACCTCTTATAGt                                                  >  2:4863929/1‑150 (MQ=255)
                                                                                                    tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAAAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATAACAACCTAATTTCACCTCCTCTTagagg                                                  >  2:1262683/1‑149 (MQ=255)
                                                                                                      tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                <  2:4610737/150‑1 (MQ=255)
                                                                                                         ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAATACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTATAGTGTCta                                             >  2:4904229/1‑150 (MQ=255)
                                                                                                         ttATAAATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                             <  1:3152035/150‑1 (MQ=255)
                                                                                                            taCATAACTGTCGTTTTTTTAATTTATAAATTAATTCAAAGATATCATGAGAATAGCGATGTAAAGCACTTAGTTTATATGGCGCGCCATTCATGGCGCGCACAAGCTATAATACCTACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                          <  1:2208306/150‑1 (MQ=255)
                                                                                                            taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAACGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAACTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCGCCTCTTAGAGTGACTatat                                          <  1:4939532/150‑1 (MQ=255)
                                                                                                            taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                          <  1:3826203/150‑1 (MQ=255)
                                                                                                                tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                      <  2:2298225/150‑1 (MQ=255)
                                                                                                                 aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                                     <  1:4038566/150‑1 (MQ=255)
                                                                                                                 aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                                     <  1:4038618/150‑1 (MQ=255)
                                                                                                                         tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                             >  1:622014/1‑150 (MQ=255)
                                                                                                                            ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                          <  1:3493381/150‑1 (MQ=255)
                                                                                                                             tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         <  1:5016632/150‑1 (MQ=255)
                                                                                                                             tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         <  2:1638249/150‑1 (MQ=255)
                                                                                                                              ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCGTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                        >  2:4771228/1‑150 (MQ=255)
                                                                                                                              ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                        >  1:4749400/1‑150 (MQ=255)
                                                                                                                              ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                        >  2:5463567/1‑150 (MQ=255)
                                                                                                                               tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                       >  2:2985280/1‑150 (MQ=255)
                                                                                                                                aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                      >  2:4762465/1‑150 (MQ=255)
                                                                                                                                aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                      >  2:5134236/1‑150 (MQ=255)
                                                                                                                                   ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                   >  1:4883777/1‑150 (MQ=255)
                                                                                                                                    tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  >  1:735803/1‑150 (MQ=255)
                                                                                                                                    tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  >  2:1032933/1‑150 (MQ=255)
                                                                                                                                    tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  >  2:532688/1‑150 (MQ=255)
                                                                                                                                    tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  >  2:1032981/1‑150 (MQ=255)
                                                                                                                                    taaaaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAGTGa                  >  2:3525153/4‑150 (MQ=255)
                                                                                                                                        aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt              >  1:2714395/1‑150 (MQ=255)
                                                                                                                                          ataataCAAACAAAACAACACAATAGCGATCTAAACCACTAAGATTAAATTGCGCCCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa            <  1:2466417/150‑1 (MQ=255)
                                                                                                                                              ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg        >  2:414630/1‑150 (MQ=255)
                                                                                                                                                 aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  2:295453/1‑150 (MQ=255)
                                                                                                                                                 aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCAAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCATGATAGAATTGCGGTATTGACTATCAAATGccc     >  1:37831/1‑150 (MQ=255)
                                                                                                                                                  aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    >  1:731953/1‑150 (MQ=255)
                                                                                                                                                  aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    >  2:1622231/1‑150 (MQ=255)
                                                                                                                                                   aGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATCATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTg   >  2:4669109/1‑150 (MQ=255)
                                                                                                                                                   aGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTg   >  2:1443631/1‑150 (MQ=255)
                                                                                                                                                    gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa  <  2:2683636/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470283‑2470580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: