Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,768,609 T→C 5.1% intergenic (+36/‑56) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,768,6090TC 5.1% 1254.9 / 37.2 372intergenic (+36/‑56)yfjP/yfjQCP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (155/197);  new base C (8/11);  total (164/208)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 381 total aligned reads displayed.

CTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCG  >  NC_000913/2768460‑2768758
                                                                                                                                                     |                                                                                                                                                     
cTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGGTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTCTTATTCTCCGAGCCCTGt                                                                                                                                                       >  1:3954766/1‑150 (MQ=255)
 tCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTc                                                                                                                                                      <  2:2484003/150‑1 (MQ=255)
 tCGATACGGTAAGCACCTTTCCCCTAATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTc                                                                                                                                                      <  2:3156822/150‑1 (MQ=255)
   gATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGc                                                                                                                                                    >  2:3067169/1‑150 (MQ=255)
        ggTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTg                                                                                                                                               >  2:336318/1‑150 (MQ=255)
            aGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGc                                                                                                                                           <  1:1770762/150‑1 (MQ=255)
              cACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAg                                                                                                                                         >  1:4045693/1‑150 (MQ=255)
               aCCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAgg                                                                                                                                        <  1:4594535/150‑1 (MQ=255)
                ccTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGCCGCACTTGCGACAggg                                                                                                                                       <  1:5396543/150‑1 (MQ=255)
                    tCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGcttt                                                                                                                                   <  1:4497132/150‑1 (MQ=255)
                     ccccTTCTTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttc                                                                                                                                  <  2:3421679/150‑1 (MQ=255)
                     ccccTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttc                                                                                                                                  <  2:2378745/150‑1 (MQ=255)
                       ccTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttctt                                                                                                                                <  1:658690/150‑1 (MQ=255)
                       ccTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttctt                                                                                                                                >  1:4229350/1‑150 (MQ=255)
                         ttATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTa                                                                                                                              <  2:5158722/150‑1 (MQ=255)
                            ttCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGCTTTCTTCTTCTGAGTGTTGAATTCTGCCCGCTTATTCTCCGAGCCCTGTCGCACGTGCGGCAGGGCTGTCTGTAttt                                                                                                                           >  2:1979227/1‑150 (MQ=25)
                             tCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTg                                                                                                                          <  2:2125118/150‑1 (MQ=255)
                              cccGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGt                                                                                                                         >  1:2329166/1‑150 (MQ=255)
                               ccGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGtt                                                                                                                        >  1:2451623/1‑150 (MQ=255)
                               ccGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGtt                                                                                                                        <  2:1072728/150‑1 (MQ=255)
                               ccGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTAGTATTCTCCGAGCCCTGTCGCAAGTGCGGCCGGGCTTTCTTTATTTGtt                                                                                                                        >  2:2647982/1‑150 (MQ=255)
                                cGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGttt                                                                                                                       <  1:297763/150‑1 (MQ=255)
                                cGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGttt                                                                                                                       >  2:2421328/1‑150 (MQ=255)
                                  ccccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTa                                                                                                                     >  2:1098374/1‑150 (MQ=255)
                                      ggTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCa                                                                                                                 <  2:1409774/150‑1 (MQ=255)
                                          cGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACt                                                                                                             <  1:2063033/150‑1 (MQ=255)
                                           ggACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTg                                                                                                            >  1:3900276/1‑150 (MQ=255)
                                            gACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTga                                                                                                           <  1:1164008/150‑1 (MQ=255)
                                               cATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgagga                                                                                                        <  2:476714/150‑1 (MQ=255)
                                               cATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgagga                                                                                                        <  2:4526569/150‑1 (MQ=255)
                                                    ttCAGGCCGTGCGGTCCTCGGTGGTGTCAGGGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTg                                                                                                   <  2:4395245/150‑1 (MQ=255)
                                                     tCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGc                                                                                                  <  1:5296275/150‑1 (MQ=255)
                                                       aGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCtt                                                                                                >  1:5260529/1‑150 (MQ=255)
                                                        ggCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTa                                                                                               <  1:4083237/150‑1 (MQ=255)
                                                            gTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTTATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAc                                                                                           <  1:4568243/150‑1 (MQ=255)
                                                             tGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAcc                                                                                          >  1:926658/1‑150 (MQ=255)
                                                                   ccTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTg                                                                                    <  1:441131/150‑1 (MQ=255)
                                                                     tCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGc                                                                                  >  1:3147472/1‑150 (MQ=255)
                                                                        gtggtgTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTc                                                                               <  2:2009994/150‑1 (MQ=255)
                                                                        gtggtgTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTc                                                                               <  2:1442506/150‑1 (MQ=255)
                                                                          ggtgTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTcgc                                                                             <  1:1612460/150‑1 (MQ=255)
                                                                               cAGGGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCttt                                                                        <  2:2048961/150‑1 (MQ=255)
                                                                                agttgaccGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCACTATTATTCTCCGAGCCCTTTCGCAAGTGCGGCAGGGATTTCTTTATTTTTTTTACACATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCTCTTTg                                                                       >  2:57684/7‑150 (MQ=37)
                                                                                aGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTg                                                                       >  1:2400418/1‑150 (MQ=255)
                                                                                 gTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTgg                                                                      >  2:2745860/1‑150 (MQ=255)
                                                                                  tGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGTGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgc                                                                     <  2:3302647/150‑1 (MQ=255)
                                                                                     cccGCGCTGTCTGGGAGTTCTTCTTCTGAGTGTTTAATGCTGCCCTATTCTTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTGTATTTGTTTTACCCATACGGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCt                                                                  <  2:3721036/150‑1 (MQ=38)
                                                                                        gcgcTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCa                                                               <  2:2854971/150‑1 (MQ=255)
                                                                                             gTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCt                                                          <  1:3986560/150‑1 (MQ=255)
                                                                                               cTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTa                                                        <  2:185497/150‑1 (MQ=255)
                                                                                               cTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTa                                                        <  1:1576142/150‑1 (MQ=255)
                                                                                                tGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGTCGCTGCAAATCTTAt                                                       >  1:3104152/1‑150 (MQ=255)
                                                                                                tGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTAt                                                       >  1:435832/1‑150 (MQ=255)
                                                                                                tGGGATTTCTTCTTCCGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTAt                                                       >  1:4983660/1‑150 (MQ=255)
                                                                                                 gggATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTAtt                                                      >  2:1085271/1‑150 (MQ=255)
                                                                                                 gggATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTAtt                                                      >  2:3475884/1‑150 (MQ=255)
                                                                                                    aTTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGc                                                   <  1:144043/150‑1 (MQ=255)
                                                                                                     tttCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGcc                                                  >  2:5326962/1‑150 (MQ=255)
                                                                                                      ttcttcttcTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCg                                                 <  2:4310087/150‑1 (MQ=255)
                                                                                                       tcttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGt                                                <  1:1928729/150‑1 (MQ=255)
                                                                                                       tcttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTAGTCGCCGt                                                <  2:1342895/150‑1 (MQ=255)
                                                                                                       tcttcttctGAGTGTTTAATTCTGCCCTATTAGTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGt                                                <  2:4863155/150‑1 (MQ=255)
                                                                                                         ttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGa                                              >  1:185302/1‑150 (MQ=255)
                                                                                                         ttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGa                                              >  2:282644/1‑150 (MQ=255)
                                                                                                            ttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccg                                           >  2:2775587/1‑150 (MQ=255)
                                                                                                            ttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGACTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccg                                           >  1:4880658/1‑150 (MQ=255)
                                                                                                               tGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtcc                                        <  2:1746764/150‑1 (MQ=255)
                                                                                                                gAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtccg                                       <  1:471137/150‑1 (MQ=255)
                                                                                                                gAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtccg                                       >  2:2539948/1‑150 (MQ=255)
                                                                                                                   tgtTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGACCGTTa                                    >  1:4203723/1‑150 (MQ=255)
                                                                                                                     tttAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAc                                  <  2:426442/150‑1 (MQ=255)
                                                                                                                     tttAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAc                                  <  1:2129599/150‑1 (MQ=255)
                                                                                                                      ttAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAcc                                 <  2:1610298/150‑1 (MQ=255)
                                                                                                                      ttAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAcc                                 >  1:4742693/1‑150 (MQ=255)
                                                                                                                      ttAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAcc                                 >  2:3829597/1‑150 (MQ=255)
                                                                                                                       tAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAccc                                <  1:1045746/150‑1 (MQ=255)
                                                                                                                         aTTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGt                              <  1:3709458/150‑1 (MQ=255)
                                                                                                                            cTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGaa                           <  2:5086038/150‑1 (MQ=255)
                                                                                                                            cTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGaa                           <  2:4221568/150‑1 (MQ=255)
                                                                                                                              gCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAaga                         <  1:5468400/150‑1 (MQ=255)
                                                                                                                              gCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAaga                         <  1:3821353/150‑1 (MQ=255)
                                                                                                                              gCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAaga                         <  1:2889452/150‑1 (MQ=255)
                                                                                                                                ccTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGTCTTCGCGCTTTGGCGATGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGt                       >  2:523128/1‑150 (MQ=255)
                                                                                                                                ccTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGt                       >  1:2244951/1‑150 (MQ=255)
                                                                                                                                ccTATTATTCTCCGAGCCCTGACGCAAGTGCGGCAGGGCTTTCTTTATTTGTTATACCCATACAGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGt                       >  2:4049727/1‑150 (MQ=255)
                                                                                                                                ccTAATATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGt                       >  1:2644163/1‑150 (MQ=255)
                                                                                                                                  tattatTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTa                     >  1:1118738/1‑150 (MQ=255)
                                                                                                                                       ttCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTcg                >  2:2173331/1‑150 (MQ=255)
                                                                                                                                          tcCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgt             >  1:853392/1‑150 (MQ=255)
                                                                                                                                             gAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtggt          <  1:3397690/150‑1 (MQ=255)
                                                                                                                                                cccTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGcc       >  1:4689051/1‑150 (MQ=255)
                                                                                                                                                cccTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCATGGTGcc       >  2:3127784/1‑150 (MQ=255)
                                                                                                                                                cccTGCCGCACTTGCGACAGGGCTCTCTTTATTTTTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGcc       >  1:4308269/1‑150 (MQ=21)
                                                                                                                                                 ccTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtgggtccc      <  2:732905/150‑6 (MQ=255)
                                                                                                                                                 ccTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc      >  1:1288231/1‑150 (MQ=255)
                                                                                                                                                 ccTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc      >  1:514543/1‑150 (MQ=255)
                                                                                                                                                   tGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAg    <  2:1156652/150‑1 (MQ=255)
                                                                                                                                                   tGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAg    >  1:2163870/1‑150 (MQ=255)
                                                                                                                                                    gTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCTCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGc   <  1:2569479/150‑1 (MQ=255)
                                                                                                                                                     tCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCg  >  2:2984987/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCG  >  NC_000913/2768460‑2768758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: