BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,362,581	C→A	86.3%	noncoding (46/76 nt)	pheU ←	tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,362,581	0	C	A	86.3%	376.3 / 55.0	140	noncoding (46/76 nt)	pheU	tRNA‑Phe
Reads supporting (aligned to +/- strand): ref base C (13/6); new base A (73/46); total (87/53)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 145 total aligned reads displayed.

TGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGT  >  NC_000913/4362434‑4362725

tGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGaaac                                                                                                                                                >  1:2056286/1‑150 (MQ=255)

 ggTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACg                                                                                                                                               <  2:272410/150‑1 (MQ=255)

 ggTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACg                                                                                                                                               >  1:188781/1‑150 (MQ=255)

   tGATGTGGGGAGACTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACggg                                                                                                                                             <  1:1514216/150‑1 (MQ=255)

   tGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACggg                                                                                                                                             <  1:1269309/150‑1 (MQ=255)

      tgtgGTGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGAt                                                                                                                                          <  2:1954666/150‑1 (MQ=255)

      tgtgGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGAt                                                                                                                                          <  2:1123677/150‑1 (MQ=255)

        tgGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAATCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGAttt                                                                                                                                        >  1:2135685/1‑150 (MQ=255)

        tgGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGAttt                                                                                                                                        >  1:1386826/1‑150 (MQ=255)

        tgGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGAttt                                                                                                                                        >  2:284996/1‑150 (MQ=255)

         ggggAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGTCCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGAtttt                                                                                                                                       <  2:381274/150‑1 (MQ=255)

          gggAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTc                                                                                                                                      <  1:1223063/150‑1 (MQ=255)

          gggAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTc                                                                                                                                      <  1:1570397/150‑1 (MQ=255)

          gggAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTc                                                                                                                                      >  2:2312087/1‑150 (MQ=255)

               aaTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATcc                                                                                                                                 <  1:455993/150‑1 (MQ=255)

               aaTCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATcc                                                                                                                                 <  1:456037/150‑1 (MQ=255)

                 tCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATcccc                                                                                                                               >  1:976551/1‑150 (MQ=255)

                 tCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATcccc                                                                                                                               >  1:941000/1‑150 (MQ=255)

                 tCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATcccc                                                                                                                               >  2:618947/1‑150 (MQ=255)

                      ttGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGA‑ACGGGGATTTTCAATCCCCTGctct                                                                                                                         >  1:1475537/1‑150 (MQ=255)

                             cGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGAc                                                                                                                   <  2:669289/150‑1 (MQ=255)

                             cGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGAc                                                                                                                   <  1:1419520/150‑1 (MQ=255)

                               gTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTg                                                                                                                 <  1:284996/150‑1 (MQ=255)

                                     ttGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTAt                                                                                                           >  1:298347/1‑150 (MQ=255)

                                        aTTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCg                                                                                                        <  2:1298453/150‑1 (MQ=255)

                                        aTTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGATTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCg                                                                                                        <  2:679618/150‑1 (MQ=255)

                                             gAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCaa                                                                                                   >  1:1732668/1‑150 (MQ=255)

                                                    aaCGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGgcg                                                                                            <  1:1532740/150‑1 (MQ=255)

                                                       gCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCAt                                                                                         <  1:2024868/150‑1 (MQ=255)

                                                            aaTCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAAc                                                                                    >  1:601548/1‑150 (MQ=255)

                                                               cGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGt                                                                                 >  2:418375/1‑150 (MQ=255)

                                                               cGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGt                                                                                 >  2:1892042/1‑150 (MQ=255)

                                                                 tGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGACTTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTaa                                                                               <  1:896470/150‑1 (MQ=255)

                                                                 tGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTCCCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTaa                                                                               <  2:686392/150‑1 (MQ=255)

                                                                 tGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTaa                                                                               <  1:1713533/150‑1 (MQ=255)

                                                                 tGGCGTCCAGAAACGAAAAACGGACCTCCGTGGAGGTCCTTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTaa                                                                               <  1:1145939/150‑1 (MQ=255)

                                                                    cGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCt                                                                            >  2:392991/1‑150 (MQ=255)

                                                                       ccTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGca                                                                         <  2:246758/150‑1 (MQ=255)

                                                                          gAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACAt                                                                      >  1:894661/1‑150 (MQ=255)

                                                                               gAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGt                                                                 >  2:2141722/1‑150 (MQ=255)

                                                                                aaaaaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTc                                                                <  1:2080150/150‑1 (MQ=255)

                                                                                aaaaaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTc                                                                >  2:141736/1‑150 (MQ=255)

                                                                                aaaaaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTc                                                                <  1:76663/150‑1 (MQ=255)

                                                                                 aaaaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCa                                                               >  2:90494/1‑150 (MQ=255)

                                                                                 aaaaCGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCa                                                               <  1:1150868/150‑1 (MQ=255)

                                                                                      ggACCTCCACGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTa                                                          >  2:539513/1‑150 (MQ=255)

                                                                                          cTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAAttt                                                      <  2:1950316/150‑1 (MQ=255)

                                                                                          cTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAAttt                                                      >  1:436722/1‑150 (MQ=255)

                                                                                          cTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAAttt                                                      >  1:1574595/1‑150 (MQ=255)

                                                                                          cTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAAtta                                                      >  1:2084943/1‑149 (MQ=255)

                                                                                          cTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAAACGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAAACTGCACAACTCGTCAACCTAAtat                                                      >  1:436723/1‑148 (MQ=255)

                                                                                              gTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGt                                                  >  1:614602/1‑150 (MQ=255)

                                                                                                  aGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTaaaa                                              >  2:2179045/1‑150 (MQ=255)

                                                                                                  aGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTaaaa                                              >  2:1631473/1‑150 (MQ=255)

                                                                                                      ccGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGTGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGa                                          <  1:980823/150‑1 (MQ=255)

                                                                                                      ccGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGa                                          <  1:1505775/150‑1 (MQ=255)

                                                                                                       cGTTTATATGAATTTGGTGCCCGGACTCGGAAACGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGAAATCTGCACATCTCGTCAACCTCATTTCAGTAAAAGCGAt                                         >  2:2083509/1‑150 (MQ=255)

                                                                                                       cGTGTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGAt                                         >  2:2083499/1‑150 (MQ=255)

                                                                                                        gTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGAtt                                        >  1:1002579/1‑150 (MQ=255)

                                                                                                        gTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGAtt                                        >  1:909957/1‑150 (MQ=255)

                                                                                                        gTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGAtt                                        >  1:2318648/1‑150 (MQ=255)

                                                                                                        gTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGAtt                                        >  2:1130209/1‑150 (MQ=255)

                                                                                                        gTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGAtt                                        >  1:2098720/1‑150 (MQ=255)

                                                                                                        gTTTATATGAATTTGGAGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATATCAGTAAAAGCGAta                                        >  2:815299/1‑149 (MQ=255)

                                                                                                         tttATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTc                                       >  2:1417504/1‑150 (MQ=255)

                                                                                                              atGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTg                                  >  1:670076/1‑150 (MQ=255)

                                                                                                              atGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTg                                  >  1:1865748/1‑150 (MQ=255)

                                                                                                              atGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTg                                  >  1:670069/1‑150 (MQ=255)

                                                                                                               tGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGc                                 >  1:1084959/1‑150 (MQ=255)

                                                                                                               tGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGc                                 >  1:1935009/1‑150 (MQ=255)

                                                                                                                gAATTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCt                                >  1:1253283/1‑150 (MQ=255)

                                                                                                                 aaTTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCtt                               >  1:1903585/1‑150 (MQ=255)

                                                                                                                 aaTTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCtt                               >  1:351849/1‑150 (MQ=255)

                                                                                                                 aaTTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCtt                               >  1:351853/1‑150 (MQ=255)

                                                                                                                  aTTTGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTa                              >  1:1654097/1‑150 (MQ=255)

                                                                                                                   tttGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTaa                             <  2:601548/150‑1 (MQ=255)

                                                                                                                   tttGGTGCCCGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTaa                             >  1:1621264/1‑150 (MQ=255)

                                                                                                                   tttGGTGCCCGGACTCGGAATCGAACCAAGAACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTaa                             >  2:708390/1‑150 (MQ=255)

                                                                                                                   tttGGTGCCCGGACTCGGAATCGAACCAAGAACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTaa                             >  2:2391182/1‑150 (MQ=255)

                                                                                                                   tttGGTGCCCGGACTCGGAATCGAACCAAGAACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTaa                             >  2:720933/1‑150 (MQ=255)

                                                                                                                   tttGGTGCCCGGACTCGGAATCGAACCAAGAACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTaa                             >  2:720960/1‑150 (MQ=255)

                                                                                                                          cccGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCg                      <  1:1272289/150‑1 (MQ=255)

                                                                                                                           ccGGACTCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCgg                     >  2:419436/1‑150 (MQ=255)

                                                                                                                               aCTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCaaa                 >  2:1767997/1‑150 (MQ=255)

                                                                                                                                 tCGGAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATc               >  2:320196/1‑150 (MQ=255)

                                                                                                                                   ggAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGc             >  1:1619357/1‑150 (MQ=255)

                                                                                                                                   ggAATCGAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGc             >  1:1078394/1‑150 (MQ=255)

                                                                                                                                   ggAAACGAACCAAGGAAACGGGGATTTTAAATCCCATGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTCCACATCTCGTCAACCTAATTTCAGTAAAATCGATTCAACTGCTTAAGATATCGGCAAAgcgc             >  1:1877541/1‑150 (MQ=21)

                                                                                                                                     aaTCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGctt           <  2:1952793/150‑1 (MQ=255)

                                                                                                                                     aaTCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGctt           <  2:1952778/150‑1 (MQ=255)

                                                                                                                                         gAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCttt       >  1:211566/1‑150 (MQ=255)

                                                                                                                                         gAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCttt       >  1:2245920/1‑150 (MQ=255)

                                                                                                                                         gAACCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCttt       >  2:1496110/1‑150 (MQ=255)

                                                                                                                                          aaCCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAATGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCtttt      >  1:1339504/1‑150 (MQ=255)

                                                                                                                                          aaCCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCtttt      >  1:202959/1‑150 (MQ=255)

                                                                                                                                           aCCAAGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTc     >  2:254952/1‑150 (MQ=255)

                                                                                                                                              aaGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCtgt  >  1:195181/1‑150 (MQ=255)

                                                                                                                                              aaGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCtgt  <  2:2026855/150‑1 (MQ=255)

                                                                                                                                              aaGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCtgt  <  2:1192613/150‑1 (MQ=255)

                                                                                                                                              aaGGAAACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCtgt  <  2:1192615/150‑1 (MQ=255)

TGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAATTGATTTGGAGGCAGAACGCTTAAATCGTGGCGTCCTGAAACGAAAAACGGACCTCCGTGGAGGTCCGTTTATATGAATTTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCGTAATCTGCACATCTCGTCAACCTAATTTCAGTAAAAGCGATTCAACTGCTTAAGATTGCGGCAAATCGCTTCTTTTCTGT  >  NC_000913/4362434‑4362725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`