Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,379,044 A→G 5.4% L108P (CTG→CCG)  frdD ← fumarate reductase (anaerobic), membrane anchor subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,379,0440AG 5.4% 439.3 / 10.6 131L108P (CTG→CCG) frdDfumarate reductase (anaerobic), membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base A (53/70);  new base G (3/4);  total (56/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 135 total aligned reads displayed.

GTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGC  >  NC_000913/4378895‑4379192
                                                                                                                                                     |                                                                                                                                                    
gtgGAGCAAGAGGCGGTAATTAATACGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCa                                                                                                                                                      <  1:1245798/150‑1 (MQ=255)
gtgGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCTTTTTGAACATCGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGTTTGTAACGACACCAATCAGCGTGACAACTGTCa                                                                                                                                                      <  1:1181394/150‑1 (MQ=255)
gtgGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCa                                                                                                                                                      <  1:2055253/150‑1 (MQ=255)
 tgGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAg                                                                                                                                                     <  1:1089378/150‑1 (MQ=255)
 cggcgCAAGACGCGGTAATTCATCCGGCGCAGAGCGTCGTTTTGAACATCGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCTTGACAACTGTCAg                                                                                                                                                     <  2:1616899/146‑1 (MQ=255)
   gAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCGGGa                                                                                                                                                   >  1:1259652/1‑150 (MQ=255)
   gAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCAAAGGCGGGCCGGATTTACAATGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCGGGa                                                                                                                                                   >  1:1259602/1‑150 (MQ=255)
       aaGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATATGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATagc                                                                                                                                               >  1:168560/1‑150 (MQ=255)
       aaGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATagc                                                                                                                                               >  1:168637/1‑150 (MQ=255)
       aaGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTACATACAAAAAGGCCCGCCATAGGCGGGGCGGTTTTAGATTGGCGATGCGTTAGATTGTAACGACACCAATAAGCGTGACAAATGTCAGGATagc                                                                                                                                               >  1:2354271/1‑150 (MQ=255)
         gagGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATagcag                                                                                                                                             >  2:2385402/1‑150 (MQ=255)
         gagGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACAGTCAGGATagcag                                                                                                                                             >  2:465662/1‑150 (MQ=255)
              ggTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGa                                                                                                                                        <  1:1606437/150‑1 (MQ=255)
              ggTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGa                                                                                                                                        <  1:2182373/150‑1 (MQ=255)
              ggTAATTAATAAGCCGCAGAGCGTCTTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGa                                                                                                                                        <  1:2159232/150‑1 (MQ=255)
               gTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATAAGCGTGACAACTTTCAGGATAGCAGCCAGAc                                                                                                                                       >  1:1946161/1‑150 (MQ=255)
                taattaatAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGAcc                                                                                                                                      >  2:1543389/1‑150 (MQ=255)
                   ttaatAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCCCCATAGGCGGGCCGGATTTACATTTGCGATGCGTTAGATTTTAACGACACCAATCAGCGTGACAACAGTCAGGATAGCAGCCAGACCgga                                                                                                                                   >  1:1935554/1‑148 (MQ=255)
                    taataaGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAg                                                                                                                                  <  1:566335/150‑1 (MQ=255)
                           gcgcAGTGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAAccc                                                                                                                           <  2:1651088/150‑1 (MQ=255)
                           gcgcAGAGCGTCGTTTTGAACATATGGTCTGGTTTCCATACAAACCGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAAccc                                                                                                                           <  2:1157336/150‑1 (MQ=255)
                           gcgcAGAGCGTCGTTTTGAACATATGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAAccc                                                                                                                           <  2:1157321/150‑1 (MQ=255)
                            cgcAGAGCGTCGTTTTGAACATATGGTCTGGTTTACAAACAAAACGGCCCCCCAAAGGCGGGCCCGATTTACAATTGCGATGCGTTAGATTGTAAAGACACCAAACAGCGTGACAACAGTCAGGCTAGCAGCCAGACCGTAGAAAAcccc                                                                                                                          >  1:1518698/1‑149 (MQ=255)
                              cAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCAtt                                                                                                                        <  2:267726/150‑1 (MQ=255)
                                    gtcgtTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCg                                                                                                                  <  1:1109922/150‑1 (MQ=255)
                                      cgtTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCa                                                                                                                <  2:2129774/150‑1 (MQ=255)
                                        ttttGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAAGGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGTCCGTAGAAAACCCATTTGCCCGCAgg                                                                                                              >  1:901676/1‑150 (MQ=255)
                                        ttttGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAgg                                                                                                              >  1:1591353/1‑150 (MQ=255)
                                        ttttGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGAGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAgg                                                                                                              >  1:1686123/1‑150 (MQ=255)
                                        ttttGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGAGTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACAGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAgg                                                                                                              >  1:2010965/1‑150 (MQ=255)
                                        ttttGAACATAGGGTCTGGTTTCCATAAAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGATAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAgg                                                                                                              >  2:943849/1‑150 (MQ=255)
                                         tttGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGt                                                                                                             <  2:580896/150‑1 (MQ=255)
                                         tttGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGt                                                                                                             >  1:576321/1‑150 (MQ=255)
                                          ttGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATAAGCGTGACAACAGTCAGGATAGCAGCCAGAACGTAGAAAAACCAATTGCCCGCAGGTa                                                                                                            >  1:13588/1‑150 (MQ=255)
                                           tGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTAc                                                                                                           <  1:233362/150‑1 (MQ=255)
                                              aCATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTg                                                                                                        <  2:1355891/150‑1 (MQ=255)
                                                    ggTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGAtttt                                                                                                  <  2:2033560/150‑1 (MQ=255)
                                                    ggTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGAtttt                                                                                                  <  2:966776/150‑1 (MQ=255)
                                                    ggTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGAtttt                                                                                                  <  1:1375027/150‑1 (MQ=255)
                                                      tCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGACGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCa                                                                                                >  1:857390/1‑150 (MQ=255)
                                                      tCTGGTTTCCATACAAAACGGCCCGCCAGAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCa                                                                                                <  1:1675654/150‑1 (MQ=255)
                                                          gTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATc                                                                                            <  2:1746625/150‑1 (MQ=255)
                                                               cATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCa                                                                                       <  1:2307129/150‑1 (MQ=255)
                                                                aTACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCAt                                                                                      <  1:1231923/150‑1 (MQ=255)
                                                                 tACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATc                                                                                     <  2:1842981/150‑1 (MQ=255)
                                                                  aCAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATcg                                                                                    >  2:549063/1‑150 (MQ=255)
                                                                    aaaaCGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATcgcg                                                                                  >  2:1937512/1‑150 (MQ=255)
                                                                     aaaCGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCgt                                                                                 <  2:1479810/150‑1 (MQ=255)
                                                                      aaCGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCgtg                                                                                <  2:843898/150‑1 (MQ=255)
                                                                      aaCGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCgtg                                                                                <  2:1441121/150‑1 (MQ=255)
                                                                        cGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCgtggt                                                                              <  2:254044/150‑1 (MQ=255)
                                                                        cGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCgtggt                                                                              <  2:1018638/150‑1 (MQ=255)
                                                                             cGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATa                                                                         >  2:62018/1‑150 (MQ=255)
                                                                              gCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATAc                                                                        <  2:159533/150‑1 (MQ=255)
                                                                              gCCATAGACGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATAc                                                                        <  1:541433/150‑1 (MQ=255)
                                                                               ccATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACg                                                                       >  1:932736/1‑150 (MQ=255)
                                                                                      cGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTaaa                                                                >  2:1727472/1‑150 (MQ=255)
                                                                                       gggCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAac                                                               <  1:2385402/150‑1 (MQ=255)
                                                                                        ggCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAacc                                                              >  1:326212/1‑150 (MQ=255)
                                                                                           cGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAaccaca                                                           <  2:2004534/150‑1 (MQ=255)
                                                                                           cGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAaccaca                                                           <  2:806699/150‑1 (MQ=255)
                                                                                                  actttGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGAAAACTGTCTGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGTATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGc                                                    <  1:424103/147‑1 (MQ=255)
                                                                                                  aCATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGc                                                    <  1:424115/150‑1 (MQ=255)
                                                                                                     ttGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGc                                                 >  2:1107287/1‑150 (MQ=255)
                                                                                                        gCGATGCGTTAGATTGTAACGACACCAAGCAGCGAGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACACATATGACCGCAGGTACGTGGATTATCAGATCGTGCAGCGCGTCGTGCATACGGTGTAAACCACACCACAGCGGCAGa                                              >  1:2289993/1‑150 (MQ=255)
                                                                                                              cGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGatc                                        >  1:873009/1‑150 (MQ=255)
                                                                                                                ttAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGatcat                                      >  1:86942/1‑150 (MQ=255)
                                                                                                                  aGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGAtcatca                                    <  2:1260209/150‑1 (MQ=255)
                                                                                                                  aGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGAtcatca                                    <  2:570460/150‑1 (MQ=255)
                                                                                                                  aGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGAtcatca                                    <  2:1260248/150‑1 (MQ=255)
                                                                                                                   gATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcag                                   <  2:1395856/150‑1 (MQ=255)
                                                                                                                   gATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcag                                   <  2:1791109/150‑1 (MQ=255)
                                                                                                                   gATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcag                                   <  1:283444/150‑1 (MQ=255)
                                                                                                                    aTTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcagg                                  <  2:532012/150‑1 (MQ=255)
                                                                                                                    aTTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcagg                                  <  2:447160/150‑1 (MQ=255)
                                                                                                                    aTTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcagg                                  <  2:176705/150‑1 (MQ=255)
                                                                                                                     ttGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcagga                                 >  1:1951759/1‑150 (MQ=255)
                                                                                                                      tGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcaggaa                                >  1:1153308/1‑150 (MQ=255)
                                                                                                                      tGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcaggaa                                >  2:1273275/1‑150 (MQ=255)
                                                                                                                      tGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcaggaa                                >  1:2190406/1‑150 (MQ=255)
                                                                                                                      tGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcaggaa                                >  1:1946681/1‑150 (MQ=255)
                                                                                                                          aCGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATcaggaacagg                            >  1:1318553/1‑150 (MQ=255)
                                                                                                                                  aaTCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGGAGAACGATCATCAGGAACAGGAATAcgcg                    >  2:1413020/1‑150 (MQ=255)
                                                                                                                                  aaTCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATAcgcg                    >  1:1278563/1‑150 (MQ=255)
                                                                                                                                   aTCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGACCGATCATCAGGAACAGGAATACGCGa                   >  1:1787263/1‑150 (MQ=255)
                                                                                                                                   aTCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGa                   <  2:13588/150‑1 (MQ=255)
                                                                                                                                   aTCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGa                   <  1:2394625/150‑1 (MQ=255)
                                                                                                                                   aTCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGa                   <  1:1345184/150‑1 (MQ=255)
                                                                                                                                       gCGTGACAACTGTCGGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCaa               <  2:1259602/150‑1 (MQ=255)
                                                                                                                                       gCGTGACAACTGTCGGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCaa               <  2:1259586/150‑1 (MQ=255)
                                                                                                                                       gCGTGACAACTGTCGGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCaa               <  2:418169/150‑1 (MQ=255)
                                                                                                                                       gCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCCTCAGGCACAGGAATACGCGACCaa               >  1:737587/1‑150 (MQ=255)
                                                                                                                                       gCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCaa               <  1:16221/150‑1 (MQ=255)
                                                                                                                                              aaCTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGct        >  2:1329351/1‑150 (MQ=255)
                                                                                                                                                   tCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTgcg   >  1:570495/1‑150 (MQ=255)
                                                                                                                                                    cAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTgcgc  <  2:326212/150‑1 (MQ=255)
                                                                                                                                                    cAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTgcgc  <  1:686233/150‑1 (MQ=255)
                                                                                                                                                    cAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTgcgc  <  1:148283/150‑1 (MQ=255)
                                                                                                                                                    cAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTgcgc  <  1:686235/150‑1 (MQ=255)
                                                                                                                                                    cAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTgcgc  <  2:1640788/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCTGGTTTCCATACAAAACGGCCCGCCATAGGCGGGCCGGATTTACATTGGCGATGCGTTAGATTGTAACGACACCAATCAGCGTGACAACTGTCAGGATAGCAGCCAGACCGTAGAAAACCCATTTGCCCGCAGGTACGTGGATTTTCAGATCGTGCATCGCGTGGTGCATACGGTGTAAACCACACCACAGCGGCAGAACGATCATCAGGAACAGGAATACGCGACCAATGAAGCTCTGCGC  >  NC_000913/4378895‑4379192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: