Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 658,952 T→C 6.5% G2G (GGT→GGC tatE → TatABCE protein translocation system subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913658,9520TC 6.5% 402.6 / 13.6 123G2G (GGT→GGCtatETatABCE protein translocation system subunit
Reads supporting (aligned to +/- strand):  ref base T (53/62);  new base C (4/4);  total (57/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

Only 100 of 129 total aligned reads displayed.

GCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAA  >  NC_000913/658803‑659101
                                                                                                                                                     |                                                                                                                                                     
gccgcTATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGt                                                                                                                                                       <  1:1236341/150‑1 (MQ=255)
gccgcAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGt                                                                                                                                                       <  2:994105/150‑1 (MQ=255)
    cAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAATGTATCTATGGGTgaga                                                                                                                                                   >  1:411430/1‑150 (MQ=255)
    cAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTgaga                                                                                                                                                   >  1:2382600/1‑150 (MQ=255)
    cAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTgaga                                                                                                                                                   >  1:2128579/1‑150 (MQ=255)
    cAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATATATGGGTgaga                                                                                                                                                   >  1:1097204/1‑150 (MQ=255)
          ttatGATGTTTTTTTACTCGGCGCTTGATTCAGCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACGGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGGCGGTTATATGTCAAGTTGGTATCTATGGGTGAGATTAGtt                                                                                                                                             >  2:1597967/1‑149 (MQ=255)
          ttatGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTATGGCGCGTTCTGTTGGGGGTTATTTGTCAAGTAGGTATCTTTGGGTGAGATTAGTa                                                                                                                                             >  2:1598016/1‑150 (MQ=255)
           tatGTTGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTTTGCGCGCGTCGTATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGttt                                                                                                                                            <  2:1443005/150‑3 (MQ=255)
           tatGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTAt                                                                                                                                            <  1:1279387/150‑1 (MQ=255)
           tatGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTAt                                                                                                                                            <  2:1442997/150‑1 (MQ=255)
                tgTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCa                                                                                                                                       >  2:2334997/1‑150 (MQ=255)
                tgTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCa                                                                                                                                       >  2:1332578/1‑150 (MQ=255)
                 gTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCaa                                                                                                                                      >  2:1296795/1‑150 (MQ=255)
                 gTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCaa                                                                                                                                      >  2:1296792/1‑150 (MQ=255)
                 gTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCaa                                                                                                                                      >  2:1532408/1‑150 (MQ=255)
                       ttACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAAAGACCGTTAATATTCTCTGGTTTTTAATGCGCGTTCTGTTGCCGGGTATATTTCAAGAAGGTATCTATTGGTGAGATTATTATTAACAAAatgct                                                                                                                                >  1:1593347/1‑150 (MQ=255)
                        tACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAActgctg                                                                                                                               <  2:1001244/150‑1 (MQ=255)
                          cTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGt                                                                                                                             >  2:2171797/1‑150 (MQ=255)
                           tCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTa                                                                                                                            >  2:1191145/1‑150 (MQ=255)
                           tCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGCGAGATTAGTATTACCAAACTGCTGGTa                                                                                                                            >  2:1792945/1‑150 (MQ=255)
                           tCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGCGAGATTAGTATTACCAAACTGCTGGTa                                                                                                                            >  2:1793147/1‑150 (MQ=255)
                           tCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGCGAGATTAGTATTACCAAACTGCTGGTa                                                                                                                            >  2:2029094/1‑150 (MQ=255)
                              gcgcTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGtt                                                                                                                         >  2:441582/1‑150 (MQ=255)
                              gcgcTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGtt                                                                                                                         >  2:2134972/1‑150 (MQ=255)
                                 cTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTgcg                                                                                                                      >  1:1247013/1‑150 (MQ=255)
                                  ttGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTgcgg                                                                                                                     <  2:271290/150‑1 (MQ=255)
                                  ttGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTgcgg                                                                                                                     <  2:926509/150‑1 (MQ=255)
                                        cACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTgg                                                                                                               <  1:515534/150‑1 (MQ=255)
                                        cACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTgg                                                                                                               <  1:409818/150‑1 (MQ=255)
                                           cTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCg                                                                                                            >  1:1139495/1‑150 (MQ=255)
                                           cTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCg                                                                                                            >  1:2109264/1‑150 (MQ=255)
                                           cTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCg                                                                                                            >  1:1139490/1‑150 (MQ=255)
                                           cTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCg                                                                                                            >  1:588908/1‑150 (MQ=255)
                                            ttGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGt                                                                                                           >  2:1441095/1‑150 (MQ=255)
                                            ttGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGt                                                                                                           <  1:2361931/150‑1 (MQ=255)
                                            ttGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGt                                                                                                           <  1:1366378/150‑1 (MQ=255)
                                              gTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTc                                                                                                         <  2:1305012/150‑1 (MQ=255)
                                               ttACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTct                                                                                                        <  1:655963/150‑1 (MQ=255)
                                                     aTTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGtt                                                                                                  >  1:292223/1‑150 (MQ=255)
                                                     aTTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGtt                                                                                                  <  2:1840825/150‑1 (MQ=255)
                                                     aTTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGtt                                                                                                  <  2:1586362/150‑1 (MQ=255)
                                                     aTTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGtt                                                                                                  >  1:104425/1‑150 (MQ=255)
                                                          tATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGa                                                                                             <  1:1547989/150‑1 (MQ=255)
                                                             tgtgtgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTa                                                                                          <  1:1849053/150‑1 (MQ=255)
                                                             tgtgtgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTa                                                                                          <  1:2008495/150‑1 (MQ=255)
                                                             tgtgtgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTa                                                                                          <  1:865308/150‑1 (MQ=255)
                                                               tgtgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGTGGCGCTGGTCGTTCTGCTGGTTGGGACTaag                                                                                        >  1:1273115/1‑150 (MQ=255)
                                                               tgtgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTaag                                                                                        >  2:694738/1‑150 (MQ=255)
                                                                 tgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTaagaa                                                                                      <  1:1137381/150‑1 (MQ=255)
                                                                 tgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTaagaa                                                                                      <  1:2032623/150‑1 (MQ=255)
                                                                 tgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTaagaa                                                                                      <  2:2068683/150‑1 (MQ=255)
                                                                 tgCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTaagaa                                                                                      <  1:1943776/150‑1 (MQ=255)
                                                                    gcgcgTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGtt                                                                                   >  1:1895697/1‑150 (MQ=255)
                                                                                 ccGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGc                                                                      <  1:1861887/150‑1 (MQ=255)
                                                                                   gTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCgg                                                                    <  1:1299526/150‑1 (MQ=255)
                                                                                           tctcTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTg                                                            <  2:921788/150‑1 (MQ=255)
                                                                                              ctGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAg                                                         <  2:1127619/150‑1 (MQ=255)
                                                                                               tGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGc                                                        >  1:648006/1‑150 (MQ=255)
                                                                                               tGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGc                                                        >  1:2385874/1‑150 (MQ=255)
                                                                                               tGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGc                                                        >  1:1372701/1‑150 (MQ=255)
                                                                                                ggTTTTTTAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCg                                                       <  2:497737/150‑1 (MQ=255)
                                                                                                 gTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCgg                                                      >  1:497082/1‑150 (MQ=255)
                                                                                                 gTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCgg                                                      >  1:932838/1‑150 (MQ=255)
                                                                                                 gTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCgg                                                      >  1:497098/1‑150 (MQ=255)
                                                                                                  tttttAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTTTGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGc                                                     <  1:469109/150‑1 (MQ=255)
                                                                                                  tttttAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTATGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGc                                                     <  1:1766303/150‑1 (MQ=255)
                                                                                                  tttttAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGc                                                     >  2:2060624/1‑150 (MQ=255)
                                                                                                  tttttAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGc                                                     <  1:1766331/150‑1 (MQ=255)
                                                                                                  tttttAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGc                                                     <  1:469096/150‑1 (MQ=255)
                                                                                                  tttttAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGc                                                     <  1:1111639/150‑1 (MQ=255)
                                                                                                  tttttAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGc                                                     <  2:620981/150‑1 (MQ=255)
                                                                                                  tttttAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATGAGTAGTACCAACCTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCGGGGCGGAGACCTTGGAGCGGc                                                     <  2:621021/150‑1 (MQ=255)
                                                                                                        aGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTaa                                               <  2:2081647/150‑1 (MQ=255)
                                                                                                         tgctcgTTCTTTTGCCGGTTATATGTCAAGTAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTATTTGCGGCGGTGGTCGTTATGCTGTTTGGGAATAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTaaa                                              <  2:489361/146‑1 (MQ=255)
                                                                                                          gcgcGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAg                                             >  2:422771/1‑150 (MQ=255)
                                                                                                          gcgcGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTAGGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAg                                             >  2:161657/1‑150 (MQ=255)
                                                                                                               ttCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGCGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTc                                        <  1:1684728/150‑1 (MQ=255)
                                                                                                               ttCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGCGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTc                                        <  1:2029094/150‑1 (MQ=255)
                                                                                                               ttCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGCGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTc                                        <  1:1793147/150‑1 (MQ=255)
                                                                                                               ttCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGCGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCGGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTc                                        <  1:1792945/150‑1 (MQ=255)
                                                                                                                    ttGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCaagaa                                   >  1:1459339/1‑150 (MQ=255)
                                                                                                                    ttGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCaagaa                                   <  1:1747348/150‑1 (MQ=255)
                                                                                                                    ttGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCaagaa                                   <  1:312376/150‑1 (MQ=255)
                                                                                                                    ttGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCAATAAAGGGTTCaagaa                                   >  1:950544/1‑150 (MQ=255)
                                                                                                                       ccGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGc                                <  2:1173784/150‑1 (MQ=255)
                                                                                                                       ccGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGc                                >  2:2314159/1‑150 (MQ=255)
                                                                                                                       ccGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGc                                >  1:759502/1‑150 (MQ=255)
                                                                                                                         ggTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGa                              <  2:1935397/150‑1 (MQ=255)
                                                                                                                             atatGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGatgaa                          >  1:1073931/1‑150 (MQ=255)
                                                                                                                                tGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGatgaatga                       <  1:44933/150‑1 (MQ=255)
                                                                                                                                   cAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAAtgatga                    <  2:1895697/150‑1 (MQ=255)
                                                                                                                                      gaagGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGa                 >  2:1592581/1‑150 (MQ=255)
                                                                                                                                      gaagGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGa                 >  2:1576035/1‑150 (MQ=255)
                                                                                                                                      gaagGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGa                 >  1:2182456/1‑150 (MQ=255)
                                                                                                                                        agGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGAtg               <  1:1306200/150‑1 (MQ=255)
                                                                                                                                          gTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGAtgct             >  2:1912904/1‑150 (MQ=255)
                                                                                                                                                   ggTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGaaa    <  2:2145685/150‑1 (MQ=255)
                                                                                                                                                   ggTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGaaa    <  1:2171401/150‑1 (MQ=255)
                                                                                                                                                     tGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGaaaaa  >  2:177783/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGATTAGTATTACCAAACTGCTGGTAGTTGCGGCGCTGGTCGTTCTGCTGTTTGGGACTAAGAAGTTACGTACGCTGGGCGGAGACCTTGGAGCGGCCATTAAAGGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAA  >  NC_000913/658803‑659101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: