Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 8.0% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA 8.0% 262.9 / 11.0 102noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (57/34);  new base A (5/3);  total (63/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGT  >  NC_000913/1287002‑1287297
                                                                                                                                                   |                                                                                                                                                    
tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCACTCTCGCTGGCGCACGAg                                                                                                                                                    >  2:2365451/1‑150 (MQ=255)
   ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCg                                                                                                                                                 >  2:349275/1‑150 (MQ=255)
   ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTAGCAGGCGCTCGAGTCg                                                                                                                                                 >  1:1315297/1‑150 (MQ=255)
          ggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCTCCCATCGGGTCGTTTCCTGCCGCAAAGCGCACTCGCTGGCGCACGAGGCGAAccgag                                                                                                                                          >  1:985997/1‑147 (MQ=14)
          ggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTg                                                                                                                                          >  2:981049/1‑150 (MQ=255)
          ggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTCAGCGCTTCGCCCATCGGGTCGTTGCAGGCGGCAAAGCACTCTCGCTGGCCCACGAGTCGAACCtgg                                                                                                                                          >  2:647118/1‑148 (MQ=14)
           gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGACGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTAgg                                                                                                                                         >  2:2027672/1‑150 (MQ=255)
            cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAGGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                        >  2:1966879/1‑150 (MQ=255)
                 tatTTGATTGCGGATTCGTTTGAGACTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAg                                                                                                                                   <  2:1149663/150‑1 (MQ=255)
                      gATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCGCTCGAATCGAACCTTGGTCGAAGGttat                                                                                                                              >  2:1815938/1‑148 (MQ=255)
                      gATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGGTCTCTCGCTTGCGCTCGAATCGAACCTTGGTCGAAGGTtct                                                                                                                              >  2:1815957/1‑150 (MQ=14)
                         tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCAGCGGCAACCCACTCACGCTGGCGCTCGAGTCGAACCATGGTCGAAGCttataaa                                                                                                                           >  1:570648/1‑145 (MQ=14)
                         tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCATGCGCACGAGTCGAACCTTGGTCGAAGCttataat                                                                                                                           >  1:1346363/1‑145 (MQ=255)
                         tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCACTCTCGCTGGCGCTCGATTCGAACCTTGGTCGAAGCTTCTAAt                                                                                                                           >  1:602780/1‑150 (MQ=255)
                             gATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCtt                                                                                                                       <  1:258541/150‑1 (MQ=255)
                                   cTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCACCGCTCTCTCGCTGGCGCTCGAAACGAACCTTGGTCGAAGCTTCTCATCAAGCCCCGc                                                                                                                 >  1:963454/1‑150 (MQ=14)
                                    ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTAGTCGAAGGTTCTCAACCTTCCCCgat                                                                                                                >  2:2141150/1‑148 (MQ=255)
                                    ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCCCACGAGTCGAACCTTGGTCGAAGCTTCACATCCTTCCCCGCa                                                                                                                >  2:1789804/1‑150 (MQ=255)
                                    ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTAGGGACGTTGCCTGAGGCAACGGTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAATGTTCTCAACCTTCCCCgaa                                                                                                                >  2:952841/1‑148 (MQ=11)
                                    ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCCCCCTTCGGGTCGTTGCCTGCGGCAACCCTCACTCCCATGCGCTCGAGTCGAAACTTAGTCGAAGCTTCTCAACCTTCCCCGCa                                                                                                                >  2:889121/1‑150 (MQ=14)
                                     tGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGGGGCAACGGTCGCTCGCGTTCGCTCGAGTCGAACCGTTGTCGAAGCGTCACAACCGTCCCCGCag                                                                                                               >  1:695129/1‑149 (MQ=14)
                                        gagTTCCGGGCCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                            <  1:1271190/150‑1 (MQ=255)
                                        gagTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                            <  1:1066509/150‑1 (MQ=255)
                                        gagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                            <  1:1066518/150‑1 (MQ=255)
                                          gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCa                                                                                                          >  1:798227/1‑150 (MQ=255)
                                            tCAGGGCCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCGTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                        <  1:2299959/150‑1 (MQ=255)
                                             cAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTGGGTCGAAGCGTCTCATCCTTCCCCCCCAGGGCACaa                                                                                                       >  2:2282276/1‑150 (MQ=14)
                                                     tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACTTGGTAGAAGCTTATAATACTTACCCGCATGGGCAGAATATTTAAt                                                                                               >  2:1668395/1‑150 (MQ=14)
                                                     tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGACTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGAATGGGCCGAAGATTAAAt                                                                                               >  2:1997503/1‑150 (MQ=11)
                                                     tttGAAAGTGATGGTGGTGGGGGAAGGATTAATCATCCCTTCGCCCTTCGCACTACGGGTCGAAGACTGACCCAAAGCTCTCACGCAGGCGCTCGAGACGAACCATGGAAGAAGCTTATCATCCTTCCCCCCCGGGGCAGAATATTTAAt                                                                                               >  2:333936/1‑150 (MQ=2)
                                                                 ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                   <  1:2246261/150‑1 (MQ=37)
                                                                   tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCATGGTCGAAGCTTCTCAACCATCCCCGCATGGGCAGAAAATTTAATTGCGGATTCGTTgg                                                                                 >  2:2264083/1‑150 (MQ=11)
                                                                   tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCCCAGGGGCCGAAAATTTTATTGCGGCTTCGTTgg                                                                                 >  2:2264074/1‑150 (MQ=11)
                                                                       gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGGGGCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCCGAATATTTAATTGCGGATTCGTTGGGAAg                                                                             >  2:1174675/1‑150 (MQ=255)
                                                                       gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                             <  1:1841693/150‑1 (MQ=255)
                                                                           gAAGGATGACTCAGCGCTGCGCGCTTCGCCATTCGTGTAGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         <  1:1217852/150‑1 (MQ=255)
                                                                                     tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCCGGGGCCGAACATTGACGTGCGGATTCCGTGGGCAGGTCAGGGAcggttg                                                               >  2:1173704/1‑145 (MQ=14)
                                                                                     tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                               >  2:2193319/1‑150 (MQ=255)
                                                                                     tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAAAATTTAATTGCGGATTCGGTGGGCAGTTCAGGGACTTTTg                                                               >  2:2241359/1‑150 (MQ=255)
                                                                                     tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATCTTTAAATGCGGCTTCGGTGGGAAGTTCAGGGACTTTTg                                                               >  2:1916551/1‑150 (MQ=14)
                                                                                     tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                               >  2:1853254/1‑150 (MQ=255)
                                                                                     tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCGTCTCATCCTTCCCCGCATGGGCAGAATATTTAAGTGCGGATTCGTTGGGACGGTCAGGGACGTTTg                                                               >  2:2193522/1‑150 (MQ=14)
                                                                                     tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCGTCTCAACCTTCCCCGCATGGGCAGGAAATTTAATTGCGGATTCGTTGGGAAGTTCCGGGAcggttg                                                               >  2:1854794/1‑145 (MQ=14)
                                                                                     tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGAGTCGAACCTTGGTCGAAGCATCTCATCCTTCCCCGCATGGGCACAATATTTAATTGCGGTTTCGGTGGGAAGTTCAGGGACGTTTg                                                               >  2:767693/1‑150 (MQ=14)
                                                                                       aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGaa                                                             >  2:2355247/1‑150 (MQ=255)
                                                                                        gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTCATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                            <  1:2264074/150‑1 (MQ=255)
                                                                                        gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                            <  1:2264083/150‑1 (MQ=255)
                                                                                           cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTg                                                         >  2:2139099/1‑150 (MQ=255)
                                                                                           cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACTCGCTGGCGCGCGAGTCGAACCGTGGTCGAAGCTTCACAACCATCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTg                                                         >  2:2087111/1‑150 (MQ=17)
                                                                                            tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                  <  1:727495/60‑1 (MQ=35)
                                                                                            tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                  >  2:727495/1‑60 (MQ=35)
                                                                                                    tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCACGAGTCGGACCTTGGTCGAAGCATCTCAACCTTCCCCGAAGGGGCCGTAAATTTAATTGCGGATACCATGGGACCTTAAGGGCCTTTTGAAAGTGAtggtgggg                                                >  2:1934730/1‑148 (MQ=14)
                                                                                                    tCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACTTGGTCGAAGCTTCACATCCATACCCGCAAGGGCAGAATATTTAAATTCGGATTCGTTGGGAAGTTGAGGGACTTTTGAGAgtggtggtggtg                                                >  2:1099302/1‑150 (MQ=17)
                                                                                                     cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTACTTGCg                                                                                           <  1:336741/106‑1 (MQ=12)
                                                                                                     cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                           <  1:336858/106‑1 (MQ=17)
                                                                                                     cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                           >  2:336741/1‑106 (MQ=17)
                                                                                                     cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                           >  2:336858/1‑106 (MQ=17)
                                                                                                            cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAgg                                        <  2:1956855/150‑1 (MQ=255)
                                                                                                            cGGGTCGGTGCCGGCGGCAACGCTCTCTCGCGGGCGCTCGAGGCGACCCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAAGATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAgg                                        <  2:2323891/150‑1 (MQ=14)
                                                                                                             gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                       <  2:1682189/150‑1 (MQ=255)
                                                                                                             agagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCCGAATAGGTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGAGGGTGGTGGGGGACGGa                                       >  2:1906633/5‑150 (MQ=14)
                                                                                                             agagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                       >  2:2187465/5‑150 (MQ=255)
                                                                                                                   ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCCGAATATTTAAATGAGGATTCGGTGGGAAGTTCAGGGACGTTTGAAAGTGAGGGTGGTGGGGGGAGGATTCgta                                 >  1:1850239/1‑148 (MQ=17)
                                                                                                                   ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTAGAAGCATCTAATACTTCCCCCCAAGGGGAGGATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACGTTTGTAAGTGATTGTGGTGGGGGCCGGATTCGaa                                 >  1:478723/1‑150 (MQ=17)
                                                                                                                   ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTTAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                 >  1:1284632/1‑150 (MQ=255)
                                                                                                                    tGCCTGCGGCAACGCTCTCTCGCTGGCGATAGAGTCGAACCTTCGTCTTATCTACTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGCAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                <  2:2063234/150‑1 (MQ=17)
                                                                                                                     gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCACATCCTTACCCGCAGGGGCAGAATATTTAATTTCGGATTCGTTGGGAAGTTAAGGGACTTTTGAAAGTGATGGTGGTGGGGGGAGGATTCGaaac                               >  1:1434813/1‑148 (MQ=17)
                                                                                                                      ccTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGaa                                                                                                       <  1:577556/77‑1 (MQ=21)
                                                                                                                      ccTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGaa                                                                                                       >  2:577556/1‑77 (MQ=21)
                                                                                                                      ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGaa                                                                                                       >  2:1885669/1‑77 (MQ=39)
                                                                                                                      ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGaa                                                                                                       <  1:1885669/77‑1 (MQ=39)
                                                                                                                       cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                             <  2:1406772/150‑1 (MQ=255)
                                                                                                                       cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTAAAACTTACCCGCAAGGGCAGAAAATTTAATTTCGGAATCGTTGTGAAGTTAAGGGAATTTTTAAAGTGATGGTGGTGGGGGGAGGATTCGaaaatt                             >  1:2337423/1‑146 (MQ=17)
                                                                                                                          cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                            >  2:685689/1‑84 (MQ=255)
                                                                                                                          cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                            <  1:1713401/84‑1 (MQ=255)
                                                                                                                          cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                            <  1:685689/84‑1 (MQ=255)
                                                                                                                          cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                            >  2:1713399/1‑84 (MQ=255)
                                                                                                                          cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                            >  2:1713401/1‑84 (MQ=255)
                                                                                                                          cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                            <  1:1713399/84‑1 (MQ=255)
                                                                                                                             cAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGt                       <  1:988441/150‑1 (MQ=255)
                                                                                                                                   tctctcGCTGTCGCTCGAGTCGAACGTTTGTCGAATCTTCTCATCCGTCCCCGCATTGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                 <  1:63755/150‑1 (MQ=18)
                                                                                                                                   tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                 <  1:532367/150‑1 (MQ=255)
                                                                                                                                   tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                 <  1:233553/150‑1 (MQ=255)
                                                                                                                                   tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                 <  1:233515/150‑1 (MQ=255)
                                                                                                                                   tctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                 >  2:1086366/1‑150 (MQ=255)
                                                                                                                                      ctcGCTGGCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg              <  2:637204/150‑1 (MQ=255)
                                                                                                                                      ctcGCTGGCGCTAGAGGCGCACCTCGGACTAATCCACTCAACCTTCCCCGCATGGGCACCACCACTAAGTGCGGATCCGTTGGCTATTTCAGGGCCTTTTGAAAGCGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg              <  2:1038483/150‑1 (MQ=16)
                                                                                                                                      cgcGCTGGCGCGCGCGGCGCCCCCTGGTCGAAGCGTCGCATCCGTCCCCGCATGGGCAGCCGCTTGCCTTGCGGCTTCGGTGGGACGTTCAGGGGCGTTTGGAAGGGGTGGGGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg              <  2:637199/148‑1 (MQ=12)
                                                                                                                                         gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         >  2:1217854/1‑88 (MQ=255)
                                                                                                                                         gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         >  2:1278673/1‑88 (MQ=255)
                                                                                                                                         gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         <  1:1278673/88‑1 (MQ=255)
                                                                                                                                         gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         <  1:1217854/88‑1 (MQ=255)
                                                                                                                                         gCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         <  1:1657303/88‑1 (MQ=255)
                                                                                                                                         gCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         >  2:1657303/1‑88 (MQ=255)
                                                                                                                                          cTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGa          <  1:2231398/150‑1 (MQ=255)
                                                                                                                                                cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa    <  2:1434813/150‑1 (MQ=255)
                                                                                                                                                 tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg   >  2:1077689/1‑150 (MQ=255)
                                                                                                                                                 tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg   >  2:898412/1‑150 (MQ=255)
                                                                                                                                                 tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg   >  2:898421/1‑150 (MQ=255)
                                                                                                                                                  cGAGTCGATCCTTGGTCGCAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGt  <  1:2367441/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGT  >  NC_000913/1287002‑1287297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: