Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,222 T→A 14.1% intergenic (‑183/+185) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,2220TA14.1% 271.8 / 2.2 98intergenic (‑183/+185)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base T (26/54);  new base A (15/2);  total (42/56)
Fisher's exact test for biased strand distribution p-value = 2.74e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCA  >  NC_000913/1351074‑1351370
                                                                                                                                                    |                                                                                                                                                     
tGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCATGGGGGGCGCTTTTCCCCTCACCCTAACCCtctc                                                                                                                                                      <  1:1308058/150‑1 (MQ=255)
tGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCtctc                                                                                                                                                      <  1:414761/150‑1 (MQ=255)
tGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCtctc                                                                                                                                                      <  1:1523378/150‑1 (MQ=255)
  aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTccc                                                                                                                                                    <  1:1691756/150‑1 (MQ=255)
  aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTccc                                                                                                                                                    <  2:1277629/150‑1 (MQ=255)
  aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTccc                                                                                                                                                    <  2:1277576/150‑1 (MQ=255)
  aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTccc                                                                                                                                                    <  2:1277688/150‑1 (MQ=255)
      tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                               <  2:370389/150‑1 (MQ=255)
      tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                               <  2:1092733/150‑1 (MQ=255)
           cGATTCGTCGTTTTAGTAAACAGTACGAGCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATACTTGCCAGGTCCGACCGGAGCAGGCTGCGGCTGGGGGGGCGCGTTTACCCACACCCGAACCCTCAACCCAAACGGGc                                                                                                                                          >  2:1675579/1‑150 (MQ=255)
           cGATTCGTCGTGTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTCCCCACACCCAAACCCACACCCCCCAGGGGc                                                                                                                                          >  2:1105096/1‑150 (MQ=255)
             aTTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                                                                        <  2:853279/150‑1 (MQ=255)
                cgtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAggg                                                                                                                                     <  2:381643/150‑1 (MQ=255)
                    gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAcc                                                                                                                                 <  2:2240590/150‑1 (MQ=255)
                    gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAcc                                                                                                                                 <  2:1105987/150‑1 (MQ=255)
                    gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAcc                                                                                                                                 <  2:2257206/150‑1 (MQ=255)
                    gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCACTCCCCAGAGGGGCGAGGGGAcc                                                                                                                                 >  1:2370052/1‑150 (MQ=255)
                    gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCACACCCCCCAGGGGCGAGGGGAcc                                                                                                                                 >  1:2041800/1‑150 (MQ=255)
                     ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGCGGGGACCg                                                                                                                                >  2:1792187/1‑150 (MQ=255)
                     ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCg                                                                                                                                >  2:1196150/1‑150 (MQ=255)
                     ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCg                                                                                                                                >  2:803548/1‑150 (MQ=255)
                     ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCACTCCCCAGAGGGGCGAGGGGACCg                                                                                                                                >  2:1765506/1‑150 (MQ=255)
                         aGTAAACAGTACGCACAGATAAACGGTTATGATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                            <  2:1382794/150‑1 (MQ=255)
                         aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                            <  2:668569/150‑1 (MQ=255)
                         aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                            <  2:668395/150‑1 (MQ=255)
                         aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                            <  2:749723/150‑1 (MQ=255)
                            aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                                                                         <  1:474152/150‑1 (MQ=255)
                               cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaa                                                                                                                      <  2:774331/150‑1 (MQ=255)
                               cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaa                                                                                                                      <  2:227665/150‑1 (MQ=255)
                               cAGTACGAACAGATAAACGGTTATTATAATCAACATGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaa                                                                                                                      <  2:1388600/150‑1 (MQ=255)
                                aGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCTAACCCGCTCCCCCGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                                                                     >  1:1981692/1‑150 (MQ=255)
                                 gTACGAACAGATAAACGGTTATTAAAAACAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCTGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCTCACCCCCGAGGGGCGAGGGGACCGAATAGTGCcaaa                                                                                                                    >  1:2164202/1‑149 (MQ=255)
                                     gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTg                                                                                                                <  1:903318/150‑1 (MQ=255)
                                       aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCACACCCCACAGGGGCGAGGGGACCGTATTGTGCAAATATtttt                                                                                                              >  1:1520999/1‑150 (MQ=255)
                                           aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAccc                                                                                                          >  2:602262/1‑150 (MQ=255)
                                             aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAACCCACACCCCACAGGGGCGAGGGGCCCCTAATGGGCAAAAAATTTTAACCCa                                                                                                        >  2:427860/1‑150 (MQ=255)
                                                  gTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGGCCGCACCCAAACCCACACCCCAGAGGGGGGAGGGGGGCCGATTTTTGAAAAATTTTTAACCCACcaac                                                                                                   >  1:831300/1‑150 (MQ=255)
                                                   ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCGAACCCACTCCCCACAGGGGCGCGGGGACCCTATTGTGCAAAAATTTTTAACCcaacaaaa                                                                                                  >  2:1836165/1‑148 (MQ=255)
                                                   ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCGAAACCACGACCCAGAGGGGCGAGGGGACCCGATTGTGCCAAAAATGTTAACCCACcaaca                                                                                                  >  2:1836155/1‑150 (MQ=255)
                                                   ttattaTAATCAACCTGGCTGTGAGGAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCACACCCCAGAGGGGCGAGGGGACCCGATTGTGCAAAAATTGTTACCCCCGcaaca                                                                                                  >  2:2062877/1‑150 (MQ=38)
                                                    tattatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGGATTGTGCAAATATTGTTACCCCCGcaacaa                                                                                                 >  2:387534/1‑150 (MQ=255)
                                                     attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                <  2:129277/150‑1 (MQ=255)
                                                     attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                <  2:1301393/150‑1 (MQ=255)
                                                     attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                <  2:2149552/150‑1 (MQ=255)
                                                        ataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAg                                                                                             <  1:1740099/150‑1 (MQ=255)
                                                         taATCAACCTGGCTGTGAGTAGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                            <  2:1832438/150‑1 (MQ=255)
                                                            tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCACTCCCCAGAGGGGCGAGGGGCCCGTAGTGTGCAAATATTGGTACCCCAGCACCAAACAGGCTc                                                                                         >  2:2149243/1‑150 (MQ=39)
                                                             cAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCa                                                                                        <  1:22742/150‑1 (MQ=255)
                                                                      tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGcccc                                                                               <  1:2141334/150‑1 (MQ=255)
                                                                      tgtgAGTAGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCCCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGcccc                                                                               <  2:1281840/150‑1 (MQ=255)
                                                                        tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTa                                                                             <  1:1701536/150‑1 (MQ=255)
                                                                        tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTa                                                                             <  1:1440603/150‑1 (MQ=255)
                                                                        tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTa                                                                             <  1:725855/150‑1 (MQ=255)
                                                                        tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTa                                                                             <  1:502322/150‑1 (MQ=255)
                                                                        tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCACTCCCCCGAGGGGCGAGGGGACCCAATTGTGCAAATAATGTTACCCCACCAACAAACAGGCTCATACAGCCCCTa                                                                             >  2:2247422/1‑150 (MQ=255)
                                                                          aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCGAAACCTCACCCCACAGGGGCCGGGGGCCCCGATTGTGCAAAAATTTTTAACCCACCAAAAAAAAGGCGCAAAAAGCCCCaaaa                                                                           >  2:644251/1‑149 (MQ=255)
                                                                          aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCAAACCCAAAACCTCACCCCAGAGGGGCGAGGGGACCCGAGTGTGCAAAAAATTTTAACCCACCCACAAACAAGCTCAAACAACCCCAAAc                                                                           >  2:644252/1‑150 (MQ=255)
                                                                               cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCACTCCCCCGAGGGGGGAGGGGACCGGATTGTGCAAAAATTTTTAACCCAGCAACAAACAGGCGCAGACAGCCCCGAACCCctt                                                                      >  2:1222605/1‑150 (MQ=38)
                                                                               cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCACACCCCCGAGGGGCGAGGGGACCGGATTGGGCCAATATTGTTACCCCAGCAACAAACAGGCTCAAACAGCCCCCAACCCgtt                                                                      >  2:2121595/1‑150 (MQ=255)
                                                                               cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTACCCACAACCAAAAACACACCCCAGGCGGGGCGGGGGGCCGGATTGTGGAAATAATTTTTCCCCACCAAAAAACAGGGGCAAAAAGACCCCAAACCttt                                                                      >  1:1240561/1‑150 (MQ=255)
                                                                                tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTc                                                                     <  1:1105096/150‑1 (MQ=255)
                                                                                tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTc                                                                     <  1:1124293/150‑1 (MQ=255)
                                                                                tataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTc                                                                     <  1:1105301/150‑1 (MQ=255)
                                                                                 ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCGAACCCAAACCCACACCCCACAGGGGCGCGGGGACCCGATTGTGCAAAAAATTTTAACCCAGCCACAAACAGGCGCATACAGCCCCAAAACCattaa                                                                    >  2:436957/1‑148 (MQ=255)
                                                                                 ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCa                                                                    <  2:2361345/150‑1 (MQ=255)
                                                                                 ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCa                                                                    <  2:2307228/150‑1 (MQ=255)
                                                                                 ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCa                                                                    <  2:909358/150‑1 (MQ=255)
                                                                                 ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACCCCCAAAACCACAACCCACAGGGGCGCCGGGGCCGTATTTTGCAAAAAATTTTAACCCCCCAACCAAAAAGCGCAAAAAGCCCCCAAccccatca                                                                    >  1:2376379/1‑145 (MQ=255)
                                                                                 ataGATGCCAGCTCCGACCGGAGCACGCTTCGGCAGGGGGGGCGCTATTCCCCTCACCCTAACCCTCACCCCACAGGGCCGAGGGGACCGTATTGTCCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCa                                                                    <  2:1422902/150‑1 (MQ=38)
                                                                                    gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg                                                                 <  2:2060399/150‑1 (MQ=255)
                                                                                       gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCTCTCCCCAGAGGGGCGAGGGGACCGGATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGa                                                              >  2:972329/1‑150 (MQ=255)
                                                                                                    ggAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCAAGGCGATGGCTGGGACGGt                                                 >  2:2372673/1‑150 (MQ=255)
                                                                                                        cAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAg                                             <  2:2068527/150‑1 (MQ=255)
                                                                                                             tGCGGCAGGGGGGGCGCTTTTCCCCAAACCCAAAACCTCTCCCCAGAGGGGCGCGGGGACCCTAATGTGGAAATAATGTTACCCCCGCAACAAAAAGGCTCATAAAGCCCCAAACCCAATAAAGGCGAAGGCGGGGAAGGTTCAGAccat                                        >  2:1832365/1‑148 (MQ=255)
                                                                                                               cGGCAGGGGGGGCGCTTGTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCGAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGc                                      <  2:527665/150‑1 (MQ=255)
                                                                                                                   aGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAATGTTACCCCAGCAAAAAACACGCTCAAACACCCCCTAACCCTATCATGGCGATGGCTGGGACGGTTCAGACCATGCCGaa                                  >  2:2319135/1‑150 (MQ=38)
                                                                                                                    gggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAat                                 <  2:787082/150‑1 (MQ=255)
                                                                                                                    gggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAat                                 <  2:1516218/150‑1 (MQ=255)
                                                                                                                    gggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAat                                 <  2:560856/150‑1 (MQ=255)
                                                                                                                      gggggCGCTTATCCCCTCACCCAAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAATGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGAAGGCTGGGACGGTTCAGACCTTGCCGAatat                               >  2:570044/1‑150 (MQ=255)
                                                                                                                          gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                           <  1:1196150/150‑1 (MQ=255)
                                                                                                                                 tCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCAcc                    <  2:1811294/150‑1 (MQ=255)
                                                                                                                                 accccACACCCTAACCCTCACCCCACAGGGGCGACGCCACCGAATAGACCAAATATTGTAACCCCACCAACAAACACCCTCATACAGCCCCTAACCCATTAATCGCGATGGCTGCGACGGATCAGACCTTGCCGAATATTCTCCAGCAcc                    <  2:1447674/149‑1 (MQ=255)
                                                                                                                                     cTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtct                >  2:2146412/1‑150 (MQ=255)
                                                                                                                                        aCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCa             <  1:580498/150‑1 (MQ=255)
                                                                                                                                        aCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCa             >  1:324550/1‑150 (MQ=255)
                                                                                                                                          ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATg           >  2:1900950/1‑150 (MQ=255)
                                                                                                                                          ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATg           >  2:723622/1‑150 (MQ=255)
                                                                                                                                           cTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGt          <  2:831300/150‑1 (MQ=255)
                                                                                                                                           cTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGt          <  2:831310/150‑1 (MQ=255)
                                                                                                                                            tAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtt         >  2:2033838/1‑150 (MQ=255)
                                                                                                                                             aaCCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGttt        <  1:1421148/150‑1 (MQ=255)
                                                                                                                                                ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCACATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca     <  2:2164202/150‑1 (MQ=255)
                                                                                                                                                ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca     <  2:1073520/150‑1 (MQ=255)
                                                                                                                                                 ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACCGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac    <  2:1012776/150‑1 (MQ=255)
                                                                                                                                                 ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac    <  1:727910/150‑1 (MQ=255)
                                                                                                                                                 ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac    <  1:268470/150‑1 (MQ=255)
                                                                                                                                                 ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac    >  1:2288709/1‑150 (MQ=255)
                                                                                                                                                   ctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacca  <  1:82205/150‑1 (MQ=255)
                                                                                                                                                   ctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacca  <  1:5202/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCA  >  NC_000913/1351074‑1351370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: