Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,371,830 A→T 5.2% S539C (AGT→TGT)  ycjM → alpha amylase catalytic domain family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,371,8300AT 5.2% 379.5 / 7.8 106S539C (AGT→TGT) ycjMalpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand):  ref base A (58/42);  new base T (3/3);  total (61/45)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

ATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGA  >  NC_000913/1371681‑1371979
                                                                                                                                                     |                                                                                                                                                     
ataataATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTa                                                                                                                                                       <  1:180349/150‑1 (MQ=255)
  aataatTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGt                                                                                                                                                     >  1:2284157/1‑150 (MQ=255)
      atTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAg                                                                                                                                                 >  2:189009/1‑150 (MQ=255)
      atTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAg                                                                                                                                                 >  2:2042591/1‑150 (MQ=255)
          tACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa                                                                                                                                             <  1:2014994/150‑1 (MQ=255)
          tACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGa                                                                                                                                             <  1:1951163/150‑1 (MQ=255)
              aTTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGatata                                                                                                                                         >  1:942397/1‑150 (MQ=255)
                tGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGACGTGGATCTAtt                                                                                                                                       >  2:2195302/1‑150 (MQ=255)
                tGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATAtt                                                                                                                                       >  2:1568894/1‑150 (MQ=255)
                tGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATAtt                                                                                                                                       >  2:1607415/1‑150 (MQ=255)
                tGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATAtt                                                                                                                                       >  2:365891/1‑150 (MQ=255)
                 gATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTg                                                                                                                                      >  1:1364396/1‑150 (MQ=255)
                 gATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTg                                                                                                                                      >  1:1364471/1‑150 (MQ=255)
                 gATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCAGGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTg                                                                                                                                      >  2:1408410/1‑150 (MQ=255)
                  aTACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTgg                                                                                                                                     <  1:264271/150‑1 (MQ=255)
                     cGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCTTGTAAATATTAGTAATCTGCATGGTCGGGATCTGTTTTGTGATGTTGATATATTGGGTa                                                                                                                                  >  2:1127339/1‑150 (MQ=255)
                     cGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTTGTGAAGTTGATATATTGGGTa                                                                                                                                  >  2:1465578/1‑150 (MQ=255)
                     cGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTTGTGAAGTTGATATATTGGGTa                                                                                                                                  >  2:284470/1‑150 (MQ=255)
                      gATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTaa                                                                                                                                 <  2:2218206/150‑1 (MQ=255)
                         tAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGa                                                                                                                              <  2:698847/150‑1 (MQ=255)
                         tAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGa                                                                                                                              <  2:1885858/150‑1 (MQ=255)
                         tAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGa                                                                                                                              <  2:535202/150‑1 (MQ=255)
                         tAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGa                                                                                                                              <  2:698866/150‑1 (MQ=255)
                         tAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGa                                                                                                                              <  2:698907/150‑1 (MQ=255)
                           aTTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGaaa                                                                                                                            <  1:1363900/150‑1 (MQ=255)
                           aTTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGaaa                                                                                                                            <  1:488253/150‑1 (MQ=255)
                           aTTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGaaa                                                                                                                            <  1:38776/150‑1 (MQ=255)
                                  cGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCt                                                                                                                     >  1:1438289/1‑150 (MQ=255)
                                    tAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTgc                                                                                                                   <  2:477320/150‑1 (MQ=255)
                                           tATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTg                                                                                                            <  1:2035635/150‑1 (MQ=255)
                                             ttCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGc                                                                                                          <  2:238347/150‑1 (MQ=255)
                                                cAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAgg                                                                                                       >  2:1082676/1‑150 (MQ=255)
                                                cAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTAGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAgg                                                                                                       >  2:1082592/1‑150 (MQ=255)
                                                 aagaagTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGt                                                                                                      >  1:2272942/1‑150 (MQ=255)
                                                 aagaagTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGt                                                                                                      >  1:307328/1‑150 (MQ=255)
                                                  ataagTAACTCTGATGTTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGtt                                                                                                     <  1:562453/148‑1 (MQ=255)
                                                  agaagTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGtt                                                                                                     <  1:1551725/150‑1 (MQ=255)
                                                  agaagTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGtt                                                                                                     <  1:1469647/150‑1 (MQ=255)
                                                       tAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTAtgtg                                                                                                >  1:599645/1‑150 (MQ=255)
                                                       tAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTAtgtg                                                                                                >  1:599753/1‑150 (MQ=255)
                                                       tAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTAtgtg                                                                                                >  1:599865/1‑150 (MQ=255)
                                                         aCGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGGAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGa                                                                                              >  2:2372254/1‑150 (MQ=255)
                                                         aCGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGa                                                                                              <  2:1098998/150‑1 (MQ=255)
                                                          cGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGAt                                                                                             <  1:416427/150‑1 (MQ=255)
                                                           gCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGAtt                                                                                            >  2:1270089/1‑150 (MQ=255)
                                                            cTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATta                                                                                           >  2:1698472/1‑150 (MQ=255)
                                                               aTGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTATTCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATtaaat                                                                                        <  2:1195579/150‑1 (MQ=255)
                                                               aTGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATtaaat                                                                                        >  2:808148/1‑150 (MQ=255)
                                                               aTGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATtaaat                                                                                        >  2:639041/1‑150 (MQ=255)
                                                                 ggTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATtaaataa                                                                                      <  2:1130656/150‑1 (MQ=255)
                                                                   tAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATaaaa                                                                                    <  2:2172568/150‑1 (MQ=255)
                                                                   tAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATaaaa                                                                                    <  2:32886/150‑1 (MQ=255)
                                                                   tAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATaaaa                                                                                    >  2:278561/1‑150 (MQ=255)
                                                                    aaTTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCATGTTATGTGGATTAAATaaaaa                                                                                   <  2:166543/150‑1 (MQ=255)
                                                                    aaTTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATaaaaa                                                                                   <  1:2218233/150‑1 (MQ=255)
                                                                    aaTTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATaaaaa                                                                                   <  1:1391685/150‑1 (MQ=255)
                                                                    aaTTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATaaaaa                                                                                   <  1:699327/150‑1 (MQ=255)
                                                                         tCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAAc                                                                              >  1:1711420/1‑150 (MQ=255)
                                                                         tCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAAc                                                                              >  1:1711523/1‑150 (MQ=255)
                                                                          cTGACTGGATTGTTTAATGTCAGTAAAAATATTCTGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACa                                                                             >  2:671471/1‑150 (MQ=255)
                                                                          cTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACa                                                                             >  1:1476550/1‑150 (MQ=255)
                                                                           tGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACAt                                                                            >  2:671871/1‑150 (MQ=255)
                                                                            gACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACAtc                                                                           >  2:2128990/1‑150 (MQ=255)
                                                                             aCTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACAtct                                                                          <  1:2085497/150‑1 (MQ=255)
                                                                             aCTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACAtct                                                                          >  2:102786/1‑150 (MQ=255)
                                                                              cTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACAtctc                                                                         <  2:2104384/150‑1 (MQ=255)
                                                                              cTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACAtctc                                                                         <  2:1596211/150‑1 (MQ=255)
                                                                                      tttAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTaa                                                                 >  1:847280/1‑150 (MQ=255)
                                                                                      tttAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTaa                                                                 >  1:1335799/1‑150 (MQ=255)
                                                                                      tttAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTaa                                                                 >  1:839682/1‑150 (MQ=255)
                                                                                      tttAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTaa                                                                 >  1:839688/1‑150 (MQ=255)
                                                                                         aaTGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATc                                                              >  2:2073718/1‑150 (MQ=255)
                                                                                          aTGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCa                                                             >  1:326612/1‑150 (MQ=255)
                                                                                                 tAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCg                                                      >  2:1065350/1‑150 (MQ=255)
                                                                                                 tAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCg                                                      >  2:1047146/1‑150 (MQ=255)
                                                                                                  aaaaaTATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGt                                                     <  1:195753/150‑1 (MQ=255)
                                                                                                    aaaTATTCAGCATGTAAATATTACTAATCTGCAGGGTCGGGATCTGATGAGGGAAGTTGATATATTGGGTAAGGAAATAACCCTGCGCCCCGGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGc                                                   >  2:1436906/1‑150 (MQ=255)
                                                                                                              cATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGc                                         <  2:1626687/150‑1 (MQ=255)
                                                                                                                 gTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCt                                                  <  2:1205545/138‑1 (MQ=255)
                                                                                                                 gTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCt                                                  >  1:1205545/1‑138 (MQ=255)
                                                                                                                   aaaTATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTgg                                    >  1:2244433/1‑150 (MQ=255)
                                                                                                                      tatTACTAATCTGCATGGTCGGGATCTGATTTGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGttt                                 <  1:284470/150‑1 (MQ=255)
                                                                                                                      tatTACTAATCTGCATGGTCGGGATCTGATTTGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGttt                                 <  1:1465578/150‑1 (MQ=255)
                                                                                                                      tatTACTAAACTGCATGGTCGGGATCTGATTTGTGAAGTTGATATATTGGCTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGttt                                 <  1:1127339/150‑1 (MQ=255)
                                                                                                                         tACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCAt                              >  1:37145/1‑150 (MQ=255)
                                                                                                                                       gTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCa                <  2:1673878/150‑1 (MQ=255)
                                                                                                                                       gTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCa                <  1:2127350/150‑1 (MQ=255)
                                                                                                                                         cGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAgg              >  1:1378242/1‑150 (MQ=255)
                                                                                                                                         cGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAgg              >  1:1378249/1‑150 (MQ=255)
                                                                                                                                          gggATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGc             >  1:1157342/1‑150 (MQ=255)
                                                                                                                                          gggATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGc             >  1:2239610/1‑150 (MQ=255)
                                                                                                                                           ggATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCt            >  1:832636/1‑150 (MQ=255)
                                                                                                                                           ggATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCt            <  1:849304/150‑1 (MQ=255)
                                                                                                                                           ggATATGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATAAAATAAAAAATCTTGCTGTTATCAGAACTGGTTTCATGCGCCCTGATTTCAgtat            >  1:1361941/1‑147 (MQ=255)
                                                                                                                                                 gATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTAATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAaag      >  1:2305021/1‑150 (MQ=255)
                                                                                                                                                  aTTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAaaga     >  1:2374916/1‑150 (MQ=255)
                                                                                                                                                  aTTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAaaga     >  1:2375018/1‑150 (MQ=255)
                                                                                                                                                   ttAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAaagaa    >  2:458244/1‑150 (MQ=255)
                                                                                                                                                   ttAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAaagaa    >  2:1674737/1‑150 (MQ=255)
                                                                                                                                                     aGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAagaaga  <  1:1408410/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATTCAGCATGTAAATATTACTAATCTGCATGGTCGGGATCTGATTAGTGAAGTTGATATATTGGGTAATGAAATAACGCTGCGCCCCTGGCAGGTTATGTGGATTAAATAAAAAGGAACATCTCATGATTAAATCAAAAATCGTGCTGTTATCAGCACTGGTTTCATGCGCCCTGATTTCAGGCTGTAAAGAAGA  >  NC_000913/1371681‑1371979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: