Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,743,931 C→T 12.0% P159S (CCG→TCG)  mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,743,9310CT12.0% 348.2 / 29.3 117P159S (CCG→TCG) mdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base C (49/53);  new base T (7/7);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

GTTACATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTCCT  >  NC_000913/1743785‑1744073
                                                                                                                                                  |                                                                                                                                              
gTTACATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCgg                                                                                                                                             <  1:1899950/150‑1 (MQ=255)
 ttACATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCggg                                                                                                                                            >  2:1725760/1‑150 (MQ=255)
 ttACATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCggg                                                                                                                                            >  2:410810/1‑150 (MQ=255)
  tACATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGt                                                                                                                                           <  2:469979/150‑1 (MQ=255)
   aCATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTa                                                                                                                                          >  1:1626083/1‑150 (MQ=255)
   aCATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTa                                                                                                                                          >  1:39065/1‑150 (MQ=255)
   aCATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTa                                                                                                                                          >  1:1339912/1‑150 (MQ=255)
   aCATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTa                                                                                                                                          >  1:1339688/1‑150 (MQ=255)
   aCATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTa                                                                                                                                          >  1:1247328/1‑150 (MQ=255)
   aCATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGGTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTa                                                                                                                                          >  1:1638121/1‑150 (MQ=255)
     aTTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATg                                                                                                                                        <  1:1923795/150‑1 (MQ=255)
          ccGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAAt                                                                                                                                   >  2:2078/1‑150 (MQ=255)
             cTCCATGGAAAACATCGATCCGGCTCTGGCAGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATggg                                                                                                                                <  2:1864225/150‑1 (MQ=255)
              tCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGc                                                                                                                               >  2:639695/1‑150 (MQ=255)
                 aTGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCttt                                                                                                                            <  2:525206/150‑1 (MQ=255)
                 aTGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCttt                                                                                                                            >  1:203942/1‑150 (MQ=255)
                 aTGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCttt                                                                                                                            >  1:115057/1‑150 (MQ=255)
                  tGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTCTGGTAATGGGCTTTa                                                                                                                           >  2:24837/1‑150 (MQ=255)
                  tGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTa                                                                                                                           >  2:24816/1‑150 (MQ=255)
                    gAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATc                                                                                                                         >  2:1225054/1‑150 (MQ=255)
                     aaaaCATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCg                                                                                                                        <  1:2041153/150‑1 (MQ=255)
                     aaaaCATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCg                                                                                                                        <  2:285089/150‑1 (MQ=255)
                     aaaaCATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCg                                                                                                                        <  2:1134786/150‑1 (MQ=255)
                      aaaCATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCgg                                                                                                                       >  1:570758/1‑150 (MQ=255)
                      aaaCATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCgg                                                                                                                       >  1:1134663/1‑150 (MQ=255)
                       aaCATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGc                                                                                                                      <  1:1830266/150‑1 (MQ=255)
                       aaCATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGc                                                                                                                      <  2:1263377/150‑1 (MQ=255)
                       aaCATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGc                                                                                                                      <  2:1930020/150‑1 (MQ=255)
                             gATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCctgctg                                                                                                                <  1:1026207/150‑1 (MQ=255)
                              aTCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTgg                                                                                                               >  1:1571419/1‑150 (MQ=255)
                              aTCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTgg                                                                                                               >  1:123116/1‑150 (MQ=255)
                                ccGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTg                                                                                                             <  1:550678/150‑1 (MQ=255)
                                  ggCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGaa                                                                                                           <  1:2324470/150‑1 (MQ=255)
                                  ggCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGaa                                                                                                           <  1:1080868/150‑1 (MQ=255)
                                  ggCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGaa                                                                                                           <  1:451620/150‑1 (MQ=255)
                                  ggCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGaa                                                                                                           <  1:1958974/150‑1 (MQ=255)
                                     tctGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACAt                                                                                                        >  2:273090/1‑150 (MQ=255)
                                       tGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATcc                                                                                                      <  1:1188761/150‑1 (MQ=255)
                                              cAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTCTGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGaa                                                                                               >  2:1711064/1‑150 (MQ=255)
                                              cAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGaa                                                                                               >  2:768702/1‑150 (MQ=255)
                                               aaaGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAAc                                                                                              >  1:252439/1‑150 (MQ=255)
                                                 aGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACta                                                                                            >  1:1041530/1‑150 (MQ=255)
                                                                gcgtgcgtTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATgg                                                                             <  1:563704/150‑1 (MQ=255)
                                                                  gtgcgtTGTTGTGGGGCGCGCCGGGATATCTGGTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTc                                                                           <  1:1692651/150‑1 (MQ=255)
                                                                    gcgtTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCAt                                                                         <  1:255391/150‑1 (MQ=255)
                                                                    gcgtTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCAt                                                                         <  1:847096/150‑1 (MQ=255)
                                                                    gcgtTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCAt                                                                         <  1:347921/150‑1 (MQ=255)
                                                                    gcgtTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCAt                                                                         <  1:255394/150‑1 (MQ=255)
                                                                    gcgtTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCAt                                                                         <  1:255386/150‑1 (MQ=255)
                                                                    gcgtTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCAt                                                                         <  1:1604978/150‑1 (MQ=255)
                                                                          ttgtGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGt                                                                   >  1:1774932/1‑150 (MQ=255)
                                                                                 gcgcgcCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTg                                                            <  1:273090/150‑1 (MQ=255)
                                                                                  cgcgcCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGTGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGa                                                           <  1:551130/150‑1 (MQ=255)
                                                                                  cgcgcCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGa                                                           <  2:2080748/150‑1 (MQ=255)
                                                                                  cgcgcCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGa                                                           <  2:807771/150‑1 (MQ=255)
                                                                                  cgcgcCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGATTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGa                                                           <  2:1407092/150‑1 (MQ=255)
                                                                                              tCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgt                                               <  1:189399/150‑1 (MQ=255)
                                                                                              tCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgt                                               <  1:2311188/150‑1 (MQ=255)
                                                                                              tCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgt                                               <  1:1063308/150‑1 (MQ=255)
                                                                                              tCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgt                                               >  1:331356/1‑150 (MQ=255)
                                                                                               cTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgtt                                              <  2:230932/150‑1 (MQ=255)
                                                                                               cTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgtt                                              <  1:444779/150‑1 (MQ=255)
                                                                                               cTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgtt                                              <  1:444689/150‑1 (MQ=255)
                                                                                               cTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgtt                                              <  2:1374614/150‑1 (MQ=255)
                                                                                                tGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTATGGTAATTGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgttg                                             <  2:1638121/150‑1 (MQ=255)
                                                                                                tGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgttg                                             <  2:39065/150‑1 (MQ=255)
                                                                                                tGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgttg                                             <  2:1626083/150‑1 (MQ=255)
                                                                                                tGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgttg                                             <  2:1339912/150‑1 (MQ=255)
                                                                                                tGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgttg                                             <  2:1247328/150‑1 (MQ=255)
                                                                                                tGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGTCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgttg                                             <  1:968540/150‑1 (MQ=255)
                                                                                                tGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgttg                                             >  1:298296/1‑150 (MQ=255)
                                                                                                tGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgttg                                             <  2:863588/150‑1 (MQ=255)
                                                                                                  ttcttcCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgttggt                                           >  2:1499992/1‑150 (MQ=255)
                                                                                                  ttcttcCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCgttggt                                           >  2:491464/1‑150 (MQ=255)
                                                                                                     ttcCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTtgt                                        <  1:398409/150‑1 (MQ=255)
                                                                                                      tcCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTtgtg                                       <  2:752398/150‑1 (MQ=255)
                                                                                                      tcCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTtgtg                                       <  1:1455903/150‑1 (MQ=255)
                                                                                                       ccAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTgtgg                                      <  2:413808/150‑1 (MQ=255)
                                                                                                           gTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTgtggcgtg                                  <  2:1207179/150‑1 (MQ=255)
                                                                                                            ttGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTgtggcgtgg                                 >  1:1318351/1‑150 (MQ=255)
                                                                                                                     aCCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTACTGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTgcgg                        <  1:947042/150‑1 (MQ=255)
                                                                                                                     aCCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTgcgg                        >  1:1235237/1‑150 (MQ=255)
                                                                                                                      ccAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTgcggc                       <  2:1607383/150‑1 (MQ=255)
                                                                                                                       cAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTgcggcg                      >  1:1672186/1‑150 (MQ=255)
                                                                                                                        aGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGcggcgg                     <  1:1296498/150‑1 (MQ=255)
                                                                                                                        aGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGcggcgg                     >  1:834703/1‑150 (MQ=255)
                                                                                                                                ggTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTgg             <  1:1662457/150‑1 (MQ=255)
                                                                                                                                 gTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTggg            >  2:956878/1‑150 (MQ=255)
                                                                                                                                 gTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTggg            >  2:1502221/1‑150 (MQ=255)
                                                                                                                                   cTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTc          >  1:1059723/1‑150 (MQ=255)
                                                                                                                                    tGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTTGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCa         >  2:1723232/1‑150 (MQ=255)
                                                                                                                                    tGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCa         >  1:1228425/1‑150 (MQ=255)
                                                                                                                                    tGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCGTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCa         >  2:392803/1‑150 (MQ=255)
                                                                                                                                      gCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATg       >  1:2041833/1‑150 (MQ=255)
                                                                                                                                      gCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATg       >  1:2174400/1‑150 (MQ=255)
                                                                                                                                        aaaaaCCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGtt     >  2:2253521/1‑150 (MQ=255)
                                                                                                                                          aaaCCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTcc   >  1:965054/1‑150 (MQ=255)
                                                                                                                                           aaCCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTCCt  >  2:1754812/1‑150 (MQ=255)
                                                                                                                                           aaCCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTCCt  >  2:1120553/1‑150 (MQ=255)
                                                                                                                                           aaCCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTCCt  >  1:661133/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GTTACATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGTTGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGTATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTCCT  >  NC_000913/1743785‑1744073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: