BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,980,110	T→G	5.9%	S120A (TCC→GCC)	uspC →	universal stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,980,110	0	T	G	5.9%	413.1 / 10.0	124	S120A (TCC→GCC)	uspC	universal stress protein
Reads supporting (aligned to +/- strand): ref base T (60/55); new base G (6/2); total (67/57)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

ATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAT  >  NC_000913/1979961‑1980253

aTAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCAt                                                                                                                                                 >  2:560397/1‑150 (MQ=255)

aTAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCAt                                                                                                                                                 >  2:346429/1‑150 (MQ=255)

aTAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCAt                                                                                                                                                 >  2:346423/1‑150 (MQ=255)

aTAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCAt                                                                                                                                                 >  1:88790/1‑150 (MQ=255)

aTAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCAt                                                                                                                                                 >  1:804157/1‑150 (MQ=255)

aTAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGGGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCAt                                                                                                                                                 >  1:1944134/1‑150 (MQ=255)

 tAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATc                                                                                                                                                <  2:1276781/150‑1 (MQ=255)

 tAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATc                                                                                                                                                <  1:569927/150‑1 (MQ=255)

     ttAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGc                                                                                                                                            >  2:1190555/1‑150 (MQ=255)

          tCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGc                                                                                                                                       >  1:2297612/1‑150 (MQ=255)

            aGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGa                                                                                                                                     >  2:302352/1‑150 (MQ=255)

              gATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATGTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGaaa                                                                                                                                   >  1:424515/1‑150 (MQ=255)

               aTGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGaaaa                                                                                                                                  <  2:1945035/150‑1 (MQ=255)

                         tCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGc                                                                                                                        <  1:1722077/150‑1 (MQ=255)

                            cGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTc                                                                                                                     <  2:770619/150‑1 (MQ=255)

                            cGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTc                                                                                                                     <  2:1180875/150‑1 (MQ=255)

                              ttGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCaa                                                                                                                   <  1:1026130/150‑1 (MQ=255)

                              ttGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCaa                                                                                                                   <  1:1006251/150‑1 (MQ=255)

                                   aaGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCACGTGAg                                                                                                              >  2:83834/1‑150 (MQ=255)

                                   aaGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAg                                                                                                              >  2:1389205/1‑150 (MQ=255)

                                   aaGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAg                                                                                                              >  2:1389226/1‑150 (MQ=255)

                                   aaGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAg                                                                                                              >  2:1621376/1‑150 (MQ=255)

                                   aaGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAg                                                                                                              <  2:900591/150‑1 (MQ=255)

                                      aCGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTc                                                                                                           >  1:46280/1‑150 (MQ=255)

                                      aCGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTc                                                                                                           >  1:46275/1‑150 (MQ=255)

                                      aCGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTc                                                                                                           >  1:46243/1‑150 (MQ=255)

                                      aCGTTCATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTc                                                                                                           >  1:46261/1‑150 (MQ=255)

                                             ttGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGc                                                                                                    <  1:450565/150‑1 (MQ=255)

                                                ccTACGGTGAGTTAAGCGAACATATTCTTGAAGTATGTCACAAGCATCTTTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGt                                                                                                 <  1:103931/150‑1 (MQ=255)

                                                ccTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGt                                                                                                 >  1:1860330/1‑150 (MQ=255)

                                                ccTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGt                                                                                                 >  1:1860334/1‑150 (MQ=255)

                                                ccTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGt                                                                                                 <  1:2341715/150‑1 (MQ=255)

                                                 cTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGtt                                                                                                >  1:1761472/1‑150 (MQ=255)

                                                  tACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTa                                                                                               >  1:2130357/1‑150 (MQ=255)

                                                  tACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTa                                                                                               >  1:1989835/1‑150 (MQ=255)

                                                   aCGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAg                                                                                              <  2:83770/150‑1 (MQ=255)

                                                    cGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGt                                                                                             <  1:1583006/150‑1 (MQ=255)

                                                     ggTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGtt                                                                                            <  1:302352/150‑1 (MQ=255)

                                                      gTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTc                                                                                           <  1:1298616/150‑1 (MQ=255)

                                                       tGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTcc                                                                                          <  2:143537/150‑1 (MQ=255)

                                                        gAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCa                                                                                         <  1:1174755/150‑1 (MQ=255)

                                                             aaGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTAc                                                                                    >  2:591292/1‑150 (MQ=255)

                                                               gCGAACATAGTCTGGAAGTATGTCACAAGCCTCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTGTCTTTTCG‑CGAGCCGCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGGGAGGTCGATGTGCTGTTAGTTCCACTTACgg                                                                                  <  2:1358487/150‑1 (MQ=255)

                                                                cGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTAGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCT‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGATGTTAGTTCCACTTAAggg                                                                                 >  1:1659967/1‑150 (MQ=255)

                                                                 gAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGga                                                                                <  2:229582/150‑1 (MQ=255)

                                                                    cATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGAt                                                                             >  2:1721990/1‑150 (MQ=255)

                                                                       atTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTaa                                                                          <  1:560397/150‑1 (MQ=255)

                                                                       atTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTaa                                                                          <  1:346423/150‑1 (MQ=255)

                                                                       atTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTaa                                                                          <  2:88790/150‑1 (MQ=255)

                                                                       atTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTaa                                                                          <  2:804157/150‑1 (MQ=255)

                                                                        ttCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAAc                                                                         >  2:2084789/1‑150 (MQ=255)

                                                                         tCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAAcc                                                                        <  2:2055195/150‑1 (MQ=255)

                                                                             gAAGTATGTCACAAGCATCTTTTCGATTGGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCGGCTCGGCGAAATGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTc                                                                    <  1:1694530/150‑1 (MQ=255)

                                                                             gAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTc                                                                    <  2:135199/150‑1 (MQ=255)

                                                                             gAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTc                                                                    <  2:863024/150‑1 (MQ=255)

                                                                                   tGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGc                                                              >  2:436054/1‑150 (MQ=255)

                                                                                   tGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGc                                                              >  2:2192388/1‑150 (MQ=255)

                                                                                   tGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGc                                                              >  2:2192368/1‑150 (MQ=255)

                                                                                   tGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGc                                                              >  2:585854/1‑150 (MQ=255)

                                                                                     tCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCaa                                                            >  1:50766/1‑150 (MQ=255)

                                                                                     tCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCaa                                                            >  1:717879/1‑150 (MQ=255)

                                                                                     tCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCaa                                                            >  1:50755/1‑150 (MQ=255)

                                                                                         aaGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCtt                                                        >  2:2116431/1‑150 (MQ=255)

                                                                                           gCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTg                                                      <  1:506437/150‑1 (MQ=255)

                                                                                           gCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTg                                                      >  1:71385/1‑150 (MQ=255)

                                                                                           gCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTg                                                      <  1:624828/150‑1 (MQ=255)

                                                                                           gCATCATTTCGATTTGGTGATATGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTg                                                      >  2:635088/1‑150 (MQ=255)

                                                                                            catcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTgg                                                     <  1:2164099/150‑1 (MQ=255)

                                                                                             atcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGa                                                    >  2:1317345/1‑150 (MQ=255)

                                                                                              tcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAACAGAGGTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGaa                                                   >  2:897437/1‑150 (MQ=255)

                                                                                              tcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGaa                                                   >  2:1372429/1‑150 (MQ=255)

                                                                                               catTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGaaa                                                  <  1:1676845/150‑1 (MQ=255)

                                                                                               catTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGaaa                                                  <  1:497218/150‑1 (MQ=255)

                                                                                                  ttCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGt                                               >  2:1600986/1‑150 (MQ=255)

                                                                                                  ttCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGt                                               >  2:1871777/1‑150 (MQ=255)

                                                                                                      aTTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCa                                           <  1:1769805/150‑1 (MQ=255)

                                                                                                      aTTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCa                                           <  1:1769596/150‑1 (MQ=255)

                                                                                                       tttGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCaa                                          >  1:1486566/1‑150 (MQ=255)

                                                                                                          ggTGATTTGCGGTAATAACAATCACAGTTTCTTTTAG‑CGAGCAACCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGATGTTAGTTACAATTACGGGATATTAAACGCACCTAAGCAAGCTTTTGAAAGGTAGCAACtt                                       >  1:1041847/1‑150 (MQ=255)

                                                                                                             gATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTAt                                    <  2:1174768/150‑1 (MQ=255)

                                                                                                                       aatcacaatcacAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGctgctg                          <  1:48563/150‑1 (MQ=255)

                                                                                                                       aatcacaatcacAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGctgctg                          <  1:48620/150‑1 (MQ=255)

                                                                                                                        atcacaatcacaGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTgg                         <  1:2116431/150‑1 (MQ=255)

                                                                                                                           acaatcacaGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGACAGGTAGCAACTTTATCGCGCTGCTGGCtt                      >  2:2321318/1‑150 (MQ=255)

                                                                                                                           acaatcacaGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCtt                      >  2:2321250/1‑150 (MQ=255)

                                                                                                                             aatcacaGTTTCTTTTCG‑CGAGCATCCGGCTCGGCGAAAAGAGTTATTGCCTCCAGTGAGGTCGATGTGCGGTTAGTTCCACATACGGGAGATTAACCGCTCCTACGCAAGCTTTTGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTc                    >  1:2342870/1‑150 (MQ=255)

                                                                                                                             aatcacaGTTTCTTTTAG‑CGAGCATCCGGCTCGGCGAAAAGAGTTATTGCCGCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTAAGGGAGATTAACCGCTCCTACGCAAGCTTTTGAAAGGTAGCAACTTTATCGCGCTGCAGGCttta                    >  1:2301817/1‑149 (MQ=255)

                                                                                                                                     tttCTTTTCG‑CGAGCATCCTGCTCGGCGAAATGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAATGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTc            >  2:1190176/1‑150 (MQ=255)

                                                                                                                                     tttCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTc            >  1:985485/1‑150 (MQ=255)

                                                                                                                                      ttCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcg           <  2:1631743/150‑1 (MQ=255)

                                                                                                                                      ttCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcg           <  2:1294725/150‑1 (MQ=255)

                                                                                                                                       tCTTTTCGCCGAGCAGGATGCTC‑GCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcgc          >  1:731419/1‑150 (MQ=255)

                                                                                                                                       tCTTTTCGCCGAGCAGGATGCTC‑GCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcgc          >  1:517551/1‑150 (MQ=255)

                                                                                                                                         ttttCGCCGAGCAGGATGCTC‑GCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg        >  1:2085387/1‑150 (MQ=255)

                                                                                                                                         ttttCGCCGAGCAGGATGCTC‑GCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg        >  1:1570735/1‑150 (MQ=255)

                                                                                                                                         ttttCGCCGAGCAGGATGCTC‑GCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg        >  1:1570823/1‑150 (MQ=255)

                                                                                                                                         ttttCGCCGAGCAGGATGCTC‑GCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg        >  1:329281/1‑150 (MQ=255)

                                                                                                                                         ttttCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg        >  2:1268983/1‑150 (MQ=255)

                                                                                                                                                cgAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAt  <  1:1832371/150‑1 (MQ=255)

                                                                                                                                                cgAGCAGCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAt  <  2:150111/150‑1 (MQ=255)

ATAAGTTAATTCAGGATGCGGGGTATCCCGTTGACAAGACGTTTATTGCCTACGGTGAGTTAAGCGAACATATTCTGGAAGTATGTCACAAGCATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCG‑CGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAT  >  NC_000913/1979961‑1980253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`