Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,089,203 G→T 5.9% intergenic (‑75/+8) yeeZ ← / ← yoeB putative epimerase/toxin of the YoeB‑YefM toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,089,2030GT 5.9% 541.0 / 12.4 145intergenic (‑75/+8)yeeZ/yoeBputative epimerase/toxin of the YoeB‑YefM toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (74/62);  new base T (4/5);  total (78/67)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

CAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAAT  >  NC_000913/2089055‑2089352
                                                                                                                                                    |                                                                                                                                                     
cAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTatgaatga                                                                                                                                                      >  2:703022/1‑150 (MQ=255)
      cgcgCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGt                                                                                                                                                >  2:681358/1‑150 (MQ=255)
      cgcgCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGt                                                                                                                                                >  2:817819/1‑150 (MQ=255)
       gcgcTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGtt                                                                                                                                               >  1:299820/1‑150 (MQ=255)
           tGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCaat                                                                                                                                           >  1:165771/1‑150 (MQ=255)
           tGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAAATGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCaat                                                                                                                                           >  1:607544/1‑150 (MQ=255)
              aaGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCaataat                                                                                                                                        <  1:1634111/150‑1 (MQ=255)
              aaGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCaataat                                                                                                                                        <  1:1634067/150‑1 (MQ=255)
                gTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAAGTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGa                                                                                                                                      <  2:2027554/150‑1 (MQ=255)
                   aCATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATaa                                                                                                                                   >  1:1124007/1‑150 (MQ=255)
                   aCATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATaa                                                                                                                                   >  1:1124011/1‑150 (MQ=255)
                      tCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGa                                                                                                                                >  1:399092/1‑150 (MQ=255)
                       cGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGAc                                                                                                                               >  2:1403204/1‑150 (MQ=255)
                       cGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGAc                                                                                                                               >  2:1402962/1‑150 (MQ=255)
                       cGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGAATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGAc                                                                                                                               >  2:512579/1‑150 (MQ=255)
                          cAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACAtg                                                                                                                            >  2:182253/1‑150 (MQ=255)
                          cAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCAGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACAtg                                                                                                                            >  2:1056683/1‑150 (MQ=255)
                            gCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACAtgct                                                                                                                          <  2:2353659/150‑1 (MQ=255)
                            gCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACAtgct                                                                                                                          <  2:1277498/150‑1 (MQ=255)
                            gCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACAtgct                                                                                                                          <  2:328233/150‑1 (MQ=255)
                                cATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCCTCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCaa                                                                                                                      <  2:832064/150‑1 (MQ=255)
                                 aTGCCTAACCACCCTACCCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAAt                                                                                                                     <  2:1835208/150‑1 (MQ=255)
                                   gCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGa                                                                                                                   <  2:384709/150‑1 (MQ=255)
                                    ccTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATTAATCTGTTCAATAATGATAACGACATGCTGCAATGAg                                                                                                                  >  1:1585449/1‑150 (MQ=255)
                                    ccTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATTAATCTGTTCAATAATGATAACGACATGCTGCAATGAg                                                                                                                  >  1:1585464/1‑150 (MQ=255)
                                        accaccCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGt                                                                                                              <  2:1030276/150‑1 (MQ=255)
                                            cccTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAAt                                                                                                          <  2:1551941/150‑1 (MQ=255)
                                               tAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAAtcgt                                                                                                       <  2:912939/150‑1 (MQ=255)
                                                aaCCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTTATACACCTTAGATCTATAAGGCTACGCTAGCTTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAAtcgtc                                                                                                      <  1:1882580/150‑1 (MQ=255)
                                                aaCCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAAtcgtc                                                                                                      <  1:2142024/150‑1 (MQ=255)
                                                 aCCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGAACTATAAGGCTACGGTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAAGGATAACGACATGCTGCAATGAGCAGTGAAtcgtcg                                                                                                     <  1:2381175/150‑1 (MQ=255)
                                                   ccGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGt                                                                                                   >  2:1166436/1‑150 (MQ=255)
                                                    cGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTa                                                                                                  >  1:1060952/1‑150 (MQ=255)
                                                    cGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTa                                                                                                  >  1:1480182/1‑150 (MQ=255)
                                                    cGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTa                                                                                                  >  1:273929/1‑150 (MQ=255)
                                                        aaTTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACcg                                                                                              >  1:806886/1‑150 (MQ=255)
                                                         aTTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATACGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACcgc                                                                                             <  1:1154510/150‑1 (MQ=255)
                                                            gCGACCTTTTTCTTCCGTCATCTCCTGATACACCTTAGATCTATAAGGCTACTCTTGCGTATCAAAACTGACAATTCATTCTATGAATGAATGTGTTCAATTATGATAACGACTTGCTGCAATGAGCAGTGTATCGTCGGTAACCGCGta                                                                                          <  2:825350/150‑1 (MQ=255)
                                                            gCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGta                                                                                          <  2:1613196/150‑1 (MQ=255)
                                                            gCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGta                                                                                          <  2:1983488/150‑1 (MQ=255)
                                                            gCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGta                                                                                          <  2:547677/150‑1 (MQ=255)
                                                               aCCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACCATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATAc                                                                                       >  2:637211/1‑150 (MQ=255)
                                                                  tttttCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAg                                                                                    >  2:1189781/1‑150 (MQ=255)
                                                                  tttttCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAg                                                                                    >  1:1487599/1‑150 (MQ=255)
                                                                        aTCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTg                                                                              >  2:635864/1‑150 (MQ=255)
                                                                          ccGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGct                                                                            <  2:899864/150‑1 (MQ=255)
                                                                            gTTATCGCCTGATACACCTTAGATCTATAAGGCTACGCTCGCGTATCAAAACTGACAATTCATTCTATGAATGAATCGGGTCAATAATGATCACGACATGCTGCAATGAGCAGTGAATCGTCGGGGACCGCGTATACCAGCCGGTGctcc                                                                          >  2:1708945/1‑150 (MQ=255)
                                                                               aTCTCCTGATACACCTTAGATCTATACGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCctct                                                                       <  1:2275479/150‑1 (MQ=255)
                                                                                tctcCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTg                                                                      >  2:1102462/1‑150 (MQ=255)
                                                                                     tGATACACCTTAGATCTAGAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATg                                                                 <  1:369040/150‑1 (MQ=255)
                                                                                         acacCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTc                                                             <  1:1303587/150‑1 (MQ=255)
                                                                                         acacCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTc                                                             >  1:973875/1‑150 (MQ=255)
                                                                                         acacCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTc                                                             <  1:86264/150‑1 (MQ=255)
                                                                                                  aTCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGa                                                    >  1:167662/1‑150 (MQ=255)
                                                                                                  aTCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGa                                                    >  1:657133/1‑150 (MQ=255)
                                                                                                  aTCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGa                                                    >  2:739687/1‑150 (MQ=255)
                                                                                                  aTCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGa                                                    >  2:1464232/1‑150 (MQ=255)
                                                                                                  aTCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGa                                                    >  2:1461373/1‑150 (MQ=255)
                                                                                                    cTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGaaa                                                  >  1:2327739/1‑150 (MQ=255)
                                                                                                     tataAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAAc                                                 >  2:1777415/1‑150 (MQ=255)
                                                                                                     tataAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAAc                                                 >  1:2255933/1‑150 (MQ=255)
                                                                                                     tataAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAAc                                                 >  1:2256003/1‑150 (MQ=255)
                                                                                                      ataAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTCTGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAAcc                                                >  2:1742102/1‑150 (MQ=255)
                                                                                                      ataAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAAcc                                                >  1:2317667/1‑150 (MQ=255)
                                                                                                      ataAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAAcc                                                >  1:1560676/1‑150 (MQ=255)
                                                                                                          ggCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGAc                                            <  1:1527264/150‑1 (MQ=255)
                                                                                                            cTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACaa                                          >  2:797322/1‑150 (MQ=255)
                                                                                                            cTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACaa                                          >  1:684835/1‑150 (MQ=255)
                                                                                                            cTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACaa                                          >  1:1425279/1‑150 (MQ=255)
                                                                                                            cTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACaa                                          >  1:20598/1‑150 (MQ=255)
                                                                                                            cTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACaa                                          >  1:111811/1‑150 (MQ=255)
                                                                                                             tACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACaaa                                         <  1:442401/150‑1 (MQ=255)
                                                                                                             tACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACaaa                                         <  1:2239703/150‑1 (MQ=255)
                                                                                                                  tAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATg                                    >  2:1835970/1‑150 (MQ=255)
                                                                                                                     cGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGttt                                 <  1:1403204/150‑1 (MQ=255)
                                                                                                                     cGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGttt                                 <  1:512579/150‑1 (MQ=255)
                                                                                                                        aTCAAAACTGACAATTCATTCTATGAATTAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAg                              <  2:1585449/150‑1 (MQ=255)
                                                                                                                        aTCAAAACTGACAATTCATTCTATGAATTAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAg                              <  2:1997036/150‑1 (MQ=255)
                                                                                                                         tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGACTCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAgg                             <  1:1781943/150‑1 (MQ=255)
                                                                                                                         tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAgg                             >  1:250428/1‑150 (MQ=255)
                                                                                                                         tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAgg                             <  2:2115013/150‑1 (MQ=255)
                                                                                                                          cAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAggg                            >  2:474725/1‑150 (MQ=255)
                                                                                                                          cAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAggg                            >  2:2331341/1‑150 (MQ=255)
                                                                                                                            aaaCTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGt                          >  2:1804172/1‑150 (MQ=255)
                                                                                                                            aaaCTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGt                          <  1:1910334/150‑1 (MQ=255)
                                                                                                                              aCTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTc                        <  1:42868/150‑1 (MQ=255)
                                                                                                                              aCTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTc                        <  1:1230865/150‑1 (MQ=255)
                                                                                                                              aCTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTc                        <  1:1834518/150‑1 (MQ=255)
                                                                                                                              aCTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTc                        <  1:1230904/150‑1 (MQ=255)
                                                                                                                               cTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCt                       >  2:2258204/1‑150 (MQ=255)
                                                                                                                               cTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCt                       >  2:2258126/1‑150 (MQ=255)
                                                                                                                                tGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTg                      >  1:1390148/1‑150 (MQ=255)
                                                                                                                                 gACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTgg                     >  1:2106668/1‑150 (MQ=255)
                                                                                                                                    acttcattcTATGAATGAATCTGTTCAATACTGATCACGACATGCTGCACTGCGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCtt                  <  1:839233/148‑1 (MQ=255)
                                                                                                                                    aaTTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCtt                  <  1:1948511/150‑1 (MQ=255)
                                                                                                                                     attcattcTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTc                 <  1:1920480/150‑1 (MQ=255)
                                                                                                                                       tcattcTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTccc               <  1:1166436/150‑1 (MQ=255)
                                                                                                                                             tATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTAcc         <  2:1124011/150‑1 (MQ=255)
                                                                                                                                              atgaatgaATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCt        >  1:1092716/1‑150 (MQ=255)
                                                                                                                                                    gaatCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAAt  >  1:2164744/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAAT  >  NC_000913/2089055‑2089352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: