Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,279 C→A 8.7% intergenic (‑75/+233) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2790CA 8.7% 307.5 / 3.4 102intergenic (‑75/+233)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (45/45);  new base A (10/2);  total (55/47)
Fisher's exact test for biased strand distribution p-value = 3.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

AATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTT  >  NC_000913/2177130‑2177427
                                                                                                                                                      |                                                                                                                                                    
aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAAAGATc                                                                                                                                                      >  2:1847496/1‑150 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                                                  <  1:2243217/150‑1 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                                                  <  1:2243201/150‑1 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                                                  <  1:2243192/150‑1 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                                                  <  1:683564/150‑1 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                                                  <  1:1433506/150‑1 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGAGACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                                                  <  1:843062/150‑1 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGAGACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                                                  <  1:1800914/150‑1 (MQ=255)
        aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATGAAATTATTTGTTTTTTAATATCGAGATAACAATCACAAAAAc                                                                                                                                              >  1:1502861/1‑150 (MQ=255)
          gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                                                            <  2:802937/150‑1 (MQ=255)
           ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTATATTTCAAAATAAAATGAAAATATTTTTTTTTAAATATAGAGATAACGATAACAAAAAAGAc                                                                                                                                           >  2:456250/1‑150 (MQ=255)
              gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAat                                                                                                                                        <  2:2132610/150‑1 (MQ=255)
              gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAAGAAAATATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAat                                                                                                                                        >  2:18296/1‑150 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGTTTTGAAAATATAAATAAATTATTTTTTGTTTAATATAGAGATTACGGTCACAAAAAAGACACAATGaa                                                                                                                                   >  1:871888/1‑150 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaa                                                                                                                                   >  2:2313572/1‑150 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAATAATGTAATTATTTTTTTTTTAATATCGAGATAAAGATCACAAAAAAGACAATATTaa                                                                                                                                   >  1:871899/1‑150 (MQ=255)
                         ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                                                             <  2:1533586/150‑1 (MQ=255)
                          cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                                                            >  2:1764277/1‑150 (MQ=255)
                          cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                                                            >  2:1001963/1‑150 (MQ=255)
                             cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                                                         <  2:78114/150‑1 (MQ=255)
                             cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGGCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                                                         <  2:421622/150‑1 (MQ=255)
                                gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCCCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                                                                      <  2:382431/150‑1 (MQ=255)
                                 cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTATATATCGAGAGAACGATCACAAAAACGACAATATGAAAATTATTCgaggag                                                                                                                     >  2:1605118/1‑150 (MQ=255)
                                    tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTAAAAATATAATGAAATTATTTTTTTTTTAATAAAGAGATAAAAATAACAAAAAAGACAATATTTAAAATATTAGAGGAGTgt                                                                                                                  >  1:594303/1‑149 (MQ=255)
                                      ggttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATTTCGATATAACGATAACAAACACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                <  1:1929632/149‑1 (MQ=255)
                                      ctttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                <  2:1897956/148‑1 (MQ=255)
                                      cGTTGTACAGAATCTGCTTTGTCTAAACCACGTAAATTTTCATATCCTTTCGTTTTTTTTCTATTACAAAATATAATGAAATTATTTTTTTTAAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                <  1:1406150/150‑1 (MQ=255)
                                             cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAAAGAAAATTATTCGCGGAGTGAAAGGCaaaa                                                                                                         >  2:1913491/1‑150 (MQ=255)
                                             cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTATATTTGAAAAAAAAAATAAATTATTTTTTTTTAAAAATAGAGTAAAAAATAACAAAAAAGACAAAATTTAAATTATTATAGTAGTGAAAGGGaaaa                                                                                                         >  1:2389953/1‑150 (MQ=255)
                                              aGCATCTGCTTTGTCGATACCACGTACGTTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAAAGACCAAATTAAAATTATTCGCGGAGTGAAAGGCaaaaa                                                                                                        >  2:1121355/1‑150 (MQ=255)
                                              aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAAATAAATTAATTTTTTTTAAATATAGAGATAACGAACAAAAAAACGACAAAATGAAAATTATTAGAGGAGGTTAAGGCaaaaa                                                                                                        >  2:1121450/1‑150 (MQ=255)
                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAAACAACACAAAAACGACAAAAAGAAAAATATTCGAGGAGGGAAAGGCaaaaaaaac                                                                                                    >  2:256506/1‑148 (MQ=255)
                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGAATTAAAAAAATAATTAAAATATTTTTTTTTTAATTTGGAGTAAAAGAAAAAAAAAAAGACAAAAATAAAAATATTAGAGGGGTGTAAGGCacaaaaaaa                                                                                                    >  1:2289182/1‑148 (MQ=255)
                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAAATATTAGAGGAGTGAAAGGCAAAAAAACg                                                                                                    >  2:48304/1‑150 (MQ=255)
                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTAATAGAGGAGTGAAAGGCAAAAAAACg                                                                                                    >  2:1269306/1‑150 (MQ=255)
                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACAATATGAAAAATATTCGAGGAGTGAAAGGCAAAAAAACg                                                                                                    >  2:2313070/1‑150 (MQ=255)
                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTCAAAATATAATTTAATTATTTGTTTTTTAATATAGAGATAAAGATAACAAAAAAGACAAAATGAAAATTATTAGAGGTGTGAAAGGCaaaaaaaag                                                                                                    >  2:283752/1‑148 (MQ=255)
                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGATATAACGATCACAAAAAAGACAAAATGAAAATTATTAGATGAGTGACAGGCaaaaaaaag                                                                                                    >  2:56210/1‑148 (MQ=255)
                                                   cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                                                                   <  2:2277949/150‑1 (MQ=255)
                                                    tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGTTTTAAAAAAAAAAATAAAATATTTTTTTTTTAAAATAAAGATAACGATAAAAAAAAAAACAAAAAGAAAAATATTAGAGGGGGGAGAGGCcaaaaaaaaga                                                                                                  >  1:2254047/1‑146 (MQ=255)
                                                    tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTTTTAAAAAAATAATTAAAATATTTTTTTTTTAATATAGAGATAAAGATAAAAAAAAAAAAAAAATAAAAAATATTGGGGGGGTGGGAAGCaaaaaaaaagc                                                                                                  >  1:1953196/1‑146 (MQ=255)
                                                       tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAAAATAAATAAAATATTTTTTTTTAAATATAGAGATAAACAAGAAAAAAAACAACAAAATAAAATTATTAGAGGGGTGAAAGGCaaaaaaaaagccgc                                                                                               >  1:2126922/1‑143 (MQ=255)
                                                               cacaaCGTACTTTTTATTTGCCTGTGTTTTGTTTTCTTTTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCTAGTTAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                                                       <  1:2047865/146‑1 (MQ=255)
                                                               aaCCACTTACATTTTAATATCTTTTGTTTTTTTTTCTATTTAAAATTATAATTAAATTTTTTTTTTTTAAATATCGATATACCTATCAAAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                                                       <  1:15604/149‑1 (MQ=255)
                                                                   acttaatttttCATATCCTTTTTTTTTTTTTCTTTTTAAAATTATAATTAATTTTTTTTTTTTTAATTATCTATATAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                                                   <  1:490845/143‑1 (MQ=255)
                                                                       aatttttCATATCCTGTCTTTTTTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATTTCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGt                                                                               <  1:1913491/147‑1 (MQ=255)
                                                                        ctttttCTTATCCTGTGGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCTAGATAACGTTAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                              <  2:2232557/148‑1 (MQ=255)
                                                                         aTTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGTGTGTAAGGCAAAAAAAAGGCCTCCCGAGAGGGCAGCCGTAg                                                                             >  2:2313621/1‑150 (MQ=255)
                                                                           tttCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGGGGGGTGAAAGGGAAAAAAACGGCCTCCCGGGGGGGCGGCCGTAGcc                                                                           >  2:1745195/1‑149 (MQ=255)
                                                                            ttCATATCCTGTCGTTTTTTTTCTATTTAAAAATATATTTAATTTATTTTTTTTTAAATATCGATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                                                                          <  1:2048091/150‑1 (MQ=255)
                                                                                 atCCTGTCGTTTGTTTTCTATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                                                                     <  2:1623081/150‑1 (MQ=255)
                                                                                  tCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATTAAAATTATTCGAGGAGTGAAATGCAAAAAAAAGGCCTACCCAAAGGGAAGCCGTATCAAAGTgcg                                                                    >  2:1162705/1‑150 (MQ=255)
                                                                                     tGTCGTTTGTTTTCTATTAAAAAATATAATGAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                                                 <  1:989502/150‑1 (MQ=255)
                                                                                          tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                                                            >  1:2049558/1‑150 (MQ=255)
                                                                                                   gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                                                   <  2:1233603/150‑1 (MQ=255)
                                                                                                     tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                                                 >  1:109411/1‑150 (MQ=255)
                                                                                                     tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                                                 >  1:109466/1‑150 (MQ=255)
                                                                                                     tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                                                 >  1:1281023/1‑150 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                               <  2:271286/150‑1 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                               <  1:835553/150‑1 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                               <  1:793944/150‑1 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                               <  1:1603862/150‑1 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                               <  1:1603997/150‑1 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                               <  2:271149/150‑1 (MQ=255)
                                                                                                        cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGGTGCgg                                              >  2:1650853/1‑150 (MQ=255)
                                                                                                        cAAAATAAAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAAGTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                              >  2:576536/1‑150 (MQ=255)
                                                                                                         aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                             >  1:1656394/1‑150 (MQ=255)
                                                                                                         aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                             >  2:551262/1‑150 (MQ=255)
                                                                                                         aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                             >  2:551381/1‑150 (MQ=255)
                                                                                                          aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACACTATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                            >  1:1142802/1‑150 (MQ=255)
                                                                                                          aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                            >  1:293294/1‑150 (MQ=255)
                                                                                                              ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                        <  2:1110916/150‑1 (MQ=255)
                                                                                                                     aaTTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                                 <  1:2085346/150‑1 (MQ=255)
                                                                                                                     aaTTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                                 <  2:1997402/150‑1 (MQ=255)
                                                                                                                     aaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                                 <  2:1431660/150‑1 (MQ=255)
                                                                                                                                   aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                   >  2:2350489/1‑150 (MQ=255)
                                                                                                                                   aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                   >  1:742995/1‑150 (MQ=255)
                                                                                                                                      tatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc                <  1:557631/150‑1 (MQ=255)
                                                                                                                                      tatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc                <  1:1067857/150‑1 (MQ=255)
                                                                                                                                      tatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc                <  1:557485/150‑1 (MQ=255)
                                                                                                                                        tCGAGATAACGATCACAAAAACGACCATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGCTGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt              >  2:2025659/1‑150 (MQ=255)
                                                                                                                                        tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt              >  2:1824458/1‑150 (MQ=255)
                                                                                                                                        tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt              >  2:1824441/1‑150 (MQ=255)
                                                                                                                                        tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGGTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt              >  2:1084407/1‑150 (MQ=255)
                                                                                                                                         cGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATg             >  2:129461/1‑150 (MQ=255)
                                                                                                                                         cGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATg             >  2:134222/1‑150 (MQ=255)
                                                                                                                                         cGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATg             >  2:134250/1‑150 (MQ=255)
                                                                                                                                           agaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGAt           >  1:2324014/1‑150 (MQ=255)
                                                                                                                                           agaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGAt           >  1:1105018/1‑150 (MQ=255)
                                                                                                                                             aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa         >  2:1110072/1‑150 (MQ=255)
                                                                                                                                             aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa         <  2:311210/150‑1 (MQ=255)
                                                                                                                                             aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa         >  2:554855/1‑150 (MQ=255)
                                                                                                                                              tAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAg        <  2:2126922/150‑1 (MQ=255)
                                                                                                                                                 aCGATCACAAATACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGAc      <  1:2193062/150‑1 (MQ=255)
                                                                                                                                                 aCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAgcc      <  1:1223085/150‑3 (MQ=255)
                                                                                                                                                 aCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGAc      <  1:2193095/150‑1 (MQ=255)
                                                                                                                                                   gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc    >  2:1420745/1‑150 (MQ=255)
                                                                                                                                                   gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc    >  2:590341/1‑150 (MQ=255)
                                                                                                                                                     tCACAACACCGCCAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGCTAGGGAAGCCGTAGCAACGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCtt  <  2:1635175/150‑1 (MQ=255)
                                                                                                                                                     tCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCtt  <  2:11105/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
AATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTT  >  NC_000913/2177130‑2177427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: