Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,282,830 T→C 6.9% intergenic (+29/+110) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,8300TC 6.9% 514.5 / 22.0 159intergenic (+29/+110)rplY/yejK50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base T (71/77);  new base C (2/9);  total (73/86)
Fisher's exact test for biased strand distribution p-value = 6.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

Only 100 of 160 total aligned reads displayed.

TTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCG  >  NC_000913/2282682‑2282978
                                                                                                                                                    |                                                                                                                                                    
ttCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTc                                                                                                                                                     >  1:1303241/1‑150 (MQ=255)
atCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAAAACCGCTc                                                                                                                                                     >  1:424684/2‑150 (MQ=255)
  cTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACCTCGACTTCGTTCGCGCTTAATTGCTGAAAAAGTTGTAAAAAActccgctccg                                                                                                                                                   >  1:1272655/1‑150 (MQ=255)
    aCAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcggc                                                                                                                                                 <  2:1663761/150‑1 (MQ=255)
    aCAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcggc                                                                                                                                                 <  2:1800550/150‑1 (MQ=255)
      aGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcggcgg                                                                                                                                               <  1:1950522/150‑1 (MQ=255)
      aGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcggcgg                                                                                                                                               <  2:754403/150‑1 (MQ=255)
      aGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcggcgg                                                                                                                                               <  2:1949598/150‑1 (MQ=255)
      aGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcggcgg                                                                                                                                               <  1:657099/150‑1 (MQ=255)
         gAAGTTCTGACAATCTTTGTTGACTGTAAATAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGAAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGt                                                                                                                                            <  1:463021/150‑1 (MQ=255)
          caGTTCTGACCATCGTGGTTGACGGTACAGACATCAAAGTGCCCGCTCAGGACGTACAGCGTCACCCGTACAACCCGAAGCTGCAGCACCTCGACTTCGTTCGCGCTTACTTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGtt                                                                                                                                           <  2:1514765/149‑1 (MQ=255)
          aaGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTTAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGtt                                                                                                                                           <  1:1231988/150‑1 (MQ=255)
          aaGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGtt                                                                                                                                           <  2:1919463/150‑1 (MQ=255)
          aaGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGtt                                                                                                                                           <  1:1501639/150‑1 (MQ=255)
          aaGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGtt                                                                                                                                           <  1:1243060/150‑1 (MQ=255)
          aaGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGtt                                                                                                                                           <  1:297772/150‑1 (MQ=255)
          aaGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGtt                                                                                                                                           <  1:1717596/150‑1 (MQ=255)
             ttCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGGTGTAAAAAACCCCGCTCCGGCGGGgttgtt                                                                                                                                        >  2:146196/1‑150 (MQ=255)
              tCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCATAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtttttt                                                                                                                                       <  2:946063/150‑1 (MQ=255)
                 gACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTa                                                                                                                                    <  2:1267487/150‑1 (MQ=255)
                    cATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCt                                                                                                                                 >  1:1429214/1‑150 (MQ=255)
                         ttgttgACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGa                                                                                                                            <  1:765043/150‑1 (MQ=255)
                                   tAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGa                                                                                                                  >  2:1219798/1‑150 (MQ=255)
                                      aGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGGTTTTTGTATCTGCAGATTATGCCTGATTa                                                                                                               >  1:241482/1‑150 (MQ=255)
                                      aGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGATTTTTGTATCTGCAGATTATGCCTGATTa                                                                                                               <  1:2275262/150‑1 (MQ=255)
                                          aTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTGTGTATCTGCAGATTATGCCTGATTACGGt                                                                                                           >  1:2107415/1‑150 (MQ=255)
                                             aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGACTAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAtt                                                                                                        >  1:9824/1‑150 (MQ=255)
                                             aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAtt                                                                                                        >  2:1506441/1‑150 (MQ=255)
                                             aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAtt                                                                                                        >  1:1274921/1‑150 (MQ=255)
                                             aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAtt                                                                                                        >  1:2024434/1‑150 (MQ=255)
                                              aaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTg                                                                                                       <  1:1876739/150‑1 (MQ=255)
                                                       cTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt                                                                                              <  1:1782680/150‑1 (MQ=255)
                                                       cTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt                                                                                              <  1:2236341/150‑1 (MQ=255)
                                                        tCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTg                                                                                             <  2:395709/150‑1 (MQ=255)
                                                         cAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGc                                                                                            >  2:1731535/1‑150 (MQ=255)
                                                          aGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCGGAAGAAGTTGTAAAAAACCCCGCTCCGGCGGGGGTGGGTGTATCTGCAGATTCTGCCTGAGTACGGGATTGCTATTTTTTGCa                                                                                           >  2:1552863/1‑150 (MQ=255)
                                                           ggACGTACAGCGTCACCCGTACAAACCGACGCTGCAGCACATCGACTTCGGTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAg                                                                                          <  1:860306/150‑1 (MQ=255)
                                                           ggACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAATAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAg                                                                                          <  1:759695/150‑1 (MQ=255)
                                                              cGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGcc                                                                                       <  1:121243/150‑1 (MQ=255)
                                                              cGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGcc                                                                                       >  1:70154/1‑150 (MQ=255)
                                                                tACAGCGTCACCCGTACAATCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTATTTGCTGTATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCGTGATTTCGGTATTGCTATTTTTTGCTGGCCAg                                                                                     >  2:1910031/1‑150 (MQ=255)
                                                                tACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAg                                                                                     >  2:714627/1‑150 (MQ=255)
                                                                tACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGAGTACGGTATTGCTATTTTTTGCAGGCCAg                                                                                     >  2:1668724/1‑150 (MQ=255)
                                                                  cAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTCTCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGAt                                                                                   >  1:1357673/1‑150 (MQ=255)
                                                                  cAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTCTTGCTATTTTTTGCAGGCCAGAt                                                                                   >  1:2366968/1‑150 (MQ=255)
                                                                  cAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGAt                                                                                   >  1:1187115/1‑150 (MQ=255)
                                                                  cAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGAt                                                                                   >  1:1721289/1‑150 (MQ=255)
                                                                       tCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGc                                                                              >  2:575841/1‑150 (MQ=255)
                                                                       tCACCCGTACAAACCGAAGCTGCAGCACAGCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGc                                                                              >  2:61672/1‑150 (MQ=255)
                                                                           ccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCt                                                                                                                    <  2:323982/108‑1 (MQ=255)
                                                                           ccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCt                                                                                                                    >  1:323982/1‑108 (MQ=255)
                                                                           ccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTc                                                                          >  1:2109747/1‑150 (MQ=255)
                                                                               aCAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgc                                                                      <  2:398999/150‑1 (MQ=255)
                                                                               aCAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgc                                                                      <  1:1906439/150‑1 (MQ=255)
                                                                                cAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgccg                                                                    >  1:1468148/1‑150 (MQ=255)
                                                                                  aaCCGACTCTGCTGAACATCGACTTCGTTCTCTATTAATTGCTGAATAAGTTTTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgccgc                                                                   <  1:2319202/150‑1 (MQ=255)
                                                                                  aaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgccgc                                                                   <  2:1826325/150‑1 (MQ=255)
                                                                                  aaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgccgc                                                                   <  2:1826340/150‑1 (MQ=255)
                                                                                  aaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgccgc                                                                   <  2:1091785/150‑1 (MQ=255)
                                                                                  aaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgccgc                                                                   <  1:884174/150‑1 (MQ=255)
                                                                                  aaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgccgc                                                                   <  1:299754/150‑1 (MQ=255)
                                                                                        aGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTgg                                                             <  2:1666974/150‑1 (MQ=255)
                                                                                           tGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCAt                                                          >  1:1676832/1‑150 (MQ=255)
                                                                                               gcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAg                                                      >  1:1665852/1‑150 (MQ=255)
                                                                                               gcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAg                                                      <  2:2360902/150‑1 (MQ=255)
                                                                                               gcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAg                                                      >  1:188661/1‑150 (MQ=255)
                                                                                                 acaTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa                                                    <  2:2231836/150‑1 (MQ=255)
                                                                                                 acaTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa                                                    <  2:1607428/150‑1 (MQ=255)
                                                                                                 acaTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa                                                    >  1:105294/1‑150 (MQ=255)
                                                                                                 acaTCGACTTCGTTCGCGCTTAATTACTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa                                                    <  1:2287427/150‑1 (MQ=255)
                                                                                                 acaTCCACTTCGTTCGCGCTTAATTGCTGAATAAGTAGTAAAAAACCCCGCTCAGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAATGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa                                                    >  2:1286324/1‑150 (MQ=255)
                                                                                                   aTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAaca                                                  <  1:383891/150‑1 (MQ=255)
                                                                                                       aCTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaaa                                              >  2:1896828/1‑150 (MQ=255)
                                                                                                        cTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaaaa                                             <  2:1410186/150‑1 (MQ=255)
                                                                                                        cTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaaaa                                             >  1:1005613/1‑150 (MQ=255)
                                                                                                        cTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaaaa                                             >  1:853656/1‑150 (MQ=255)
                                                                                                        cTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCGGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaaaa                                             >  1:1763156/1‑150 (MQ=255)
                                                                                                            gttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGa                                         <  2:1272209/150‑1 (MQ=255)
                                                                                                                gcgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTaa                                     >  2:102137/1‑150 (MQ=255)
                                                                                                                gcgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGATTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTaa                                     <  2:1915676/150‑1 (MQ=255)
                                                                                                                 cgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAAt                                    >  1:649660/1‑150 (MQ=255)
                                                                                                                   cTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTg                                  <  2:1851720/150‑1 (MQ=255)
                                                                                                                    ttAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgc                                 >  1:1551165/1‑150 (MQ=255)
                                                                                                                      aaTTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCCTTCAGAACACAAAACCGATTAATTgccg                               >  2:2045604/1‑150 (MQ=255)
                                                                                                                      aaTTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgccg                               >  1:1535529/1‑150 (MQ=255)
                                                                                                                      aaTTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgccg                               <  2:1295545/150‑1 (MQ=255)
                                                                                                                            tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGAc                         >  1:605202/1‑150 (MQ=255)
                                                                                                                            tGAATAAGTTGTAAAAAAACCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCAATAAGAACACAAAACCGATTAATTGCCGCCAGAc                         >  1:994449/1‑150 (MQ=255)
                                                                                                                                tAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTcc                     <  2:1906543/150‑1 (MQ=255)
                                                                                                                                tAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTcc                     <  2:1906556/150‑1 (MQ=255)
                                                                                                                                tAAGTTGTAAAAAAACCCGCTCCGGCGGGGTTTTTTGTATCTGCCGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCAACTGGCATTCAGAACACAAAAAAGATTAATTTCCGCCCGACGTcc                     >  1:386111/1‑150 (MQ=255)
                                                                                                                                 aaGTTGTAAAAAACCCCGCTCCGGCGGGTTTTTTTGTATCTGCTTATTATGCCTGATTAGGGTTTTGCTATTTTTTGCAGGCCAGATATGGCGTTCACGCCGCATCTGTCATTCAGAACACAAAACCTATTAATTGCCGCCAGACGTCCg                    <  1:900073/150‑1 (MQ=255)
                                                                                                                                 aaGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCTGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCg                    <  1:1770053/150‑1 (MQ=255)
                                                                                                                                  aGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCgg                   >  2:2327849/1‑150 (MQ=255)
                                                                                                                                      gTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCt               <  2:1429214/150‑1 (MQ=255)
                                                                                                                                        aaaaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGc             >  1:352452/1‑150 (MQ=255)
                                                                                                                                        aaaaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGc             >  2:596585/1‑150 (MQ=255)
                                                                                                                                         aaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGGCGGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCAACAGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCa            >  1:820648/1‑150 (MQ=255)
                                                                                                                                          aaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCaa           >  2:1957109/1‑150 (MQ=255)
                                                                                                                                                  gCTCCGGCGGGGATTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTcgc   <  2:1164253/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCTACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCG  >  NC_000913/2282682‑2282978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: