Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,693 A→G 6.0% intergenic (+354/‑162) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,6930AG 6.0% 384.4 / 11.6 118intergenic (+354/‑162)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base A (49/60);  new base G (3/4);  total (52/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

Only 100 of 124 total aligned reads displayed.

CGCCGTGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGT  >  NC_000913/2653544‑2653842
                                                                                                                                                     |                                                                                                                                                     
  ccgTGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCaaaa                                                                                                                                                     <  2:393145/150‑1 (MQ=255)
      gtTCTGTATTAAAATATCCTGGCTTATTATTCCGAATTCAGAAAAATATAAATAAATCCTGCTCAAAATTAAAATTTCTAACCGTTAAAATATTTTAATTAACCATGTAAATTCACTGTCCTTTAAAAAACAAAAAACCGCCAAAATCAg                                                                                                                                                 <  1:1813196/150‑1 (MQ=255)
       ttCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAgg                                                                                                                                                <  1:1455879/150‑1 (MQ=255)
       ttCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCATGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAgg                                                                                                                                                <  2:1955944/150‑1 (MQ=255)
          ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                             >  2:836569/1‑150 (MQ=255)
          ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                             >  1:573703/1‑150 (MQ=255)
           gTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGt                                                                                                                                            >  1:2340164/1‑150 (MQ=255)
             aataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAACCAAAAAACCGCCAAAATCAGGCGGttt                                                                                                                                          <  1:1811636/150‑1 (MQ=255)
               taaaataaCCTGTATTATTAGTCATAATTCATAAAAATATAAATAAATCCTGATAAAAATTAAAAATTCTAACCGGTAAAAGATATTAATTAAACTTTTAAATTCACTTTCATTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttttt                                                                                                                                        <  1:1022243/150‑1 (MQ=255)
               taaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAACCCGCCTGATTTTGGCGGttttt                                                                                                                                        <  2:2126079/150‑1 (MQ=255)
                aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  2:275118/150‑1 (MQ=255)
                aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       >  1:1263388/1‑150 (MQ=255)
                aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  1:1674144/150‑1 (MQ=255)
                aaaataaCCTGGATTATTAGTCCGAATTCAGAAAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  1:169922/150‑1 (MQ=255)
                  aataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgt                                                                                                                                     >  2:1487613/1‑150 (MQ=255)
                   ataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgtt                                                                                                                                    <  1:763057/150‑1 (MQ=255)
                     aaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                  <  1:1234809/150‑3 (MQ=39)
                     aaCCTGGCTTATTAGTCCGAATACAGAAAAATATAAATAAACCCGCCACAAAATAAAAAATTCTAACCGCTAAAAGATATTACTTAACCATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                  <  1:1903852/150‑3 (MQ=37)
                     aaCCTGCATTATTTGACCCAATACAGACAAATATAACAAAACCCCCCAAAAAAAAAAAATTTCTACCCGCTAAAATATATAACTAAAACATGAAAATACACTTTCCTTTAAAAAACAAAACACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                  <  1:1812243/150‑3 (MQ=255)
                        cTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAACGTTATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTgg                                                                                                                               <  1:374826/150‑1 (MQ=255)
                          ggCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTc                                                                                                                             >  1:912078/1‑150 (MQ=255)
                               attaGTCCGACTTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTTGTCCGGtt                                                                                                                        >  2:1017881/1‑150 (MQ=255)
                                    tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  2:1815781/1‑150 (MQ=255)
                                    tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  1:1704493/1‑150 (MQ=255)
                                    tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  2:605222/1‑150 (MQ=255)
                                    tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  2:1952593/1‑150 (MQ=255)
                                    tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  2:759430/1‑150 (MQ=255)
                                             aGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCcag                                                                                                          >  2:593978/1‑150 (MQ=255)
                                                 aaaTATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAgg                                                                                                      >  2:2217191/1‑150 (MQ=255)
                                                   atatAAATTAAGCCTGCTCAACATTAAAAATTCTACCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCACAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                    <  2:701139/150‑1 (MQ=39)
                                                   atatAAATAAATCCTGCACAAACTTACAAATTCTACCCGGTTAACGTTATGACTTACACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                    <  2:1026898/150‑1 (MQ=38)
                                                      taaataaaTCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTa                                                                                                 <  2:907120/150‑1 (MQ=255)
                                                          taaatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAAAAAAAAAACGCCAAAATCAGGAGGTTTTTTTTTGCTGGTCCGGTTCGCGGCCTTTCCAGAAGTGTGTATTAc                                                                                             >  2:880024/1‑150 (MQ=255)
                                                           aaatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTAcc                                                                                            <  1:1641089/150‑1 (MQ=255)
                                                           aaatCCTGATAAAAATTAAAAATTCTTACCGTTAAAAGATATTACTTAAACATGTATATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCAGGTTCGCGGCCTTTCCAGCAGGTTGTATTAcc                                                                                            <  1:1641183/150‑1 (MQ=38)
                                                            aatCCTGCTCAAAATTAAAAATTCTAACCGGTACAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAACAACAAAACACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCg                                                                                           <  1:1538792/150‑1 (MQ=255)
                                                                 tGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGATGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACAgtagta                                                                                      >  1:2215592/1‑150 (MQ=255)
                                                                   cTCAAAATTAAAAATTCTAACCGGTACAAGATATTACTTAAACATGTAAATTCACTGTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                    <  1:811987/150‑1 (MQ=255)
                                                                   cTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                    <  1:2282617/150‑1 (MQ=255)
                                                                      aaaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCa                                                                                 <  2:1214942/150‑1 (MQ=255)
                                                                      aaaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCa                                                                                 >  1:998704/1‑150 (MQ=255)
                                                                      aaaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCa                                                                                 >  2:842339/1‑150 (MQ=255)
                                                                        aaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAg                                                                               >  1:98077/1‑150 (MQ=255)
                                                                           tAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAgcgc                                                                            <  2:1749012/150‑1 (MQ=255)
                                                                             aaaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGt                                                                          >  2:2258438/1‑150 (MQ=255)
                                                                              aaaTTCTAACCGGTAAACGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:719472/150‑1 (MQ=255)
                                                                              aaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAACACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:80682/150‑1 (MQ=255)
                                                                              aaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:1850133/150‑1 (MQ=255)
                                                                              aaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:1917478/150‑1 (MQ=255)
                                                                                aTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtctc                                                                       >  2:1745439/1‑150 (MQ=255)
                                                                                    tAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCg                                                                   <  1:870833/150‑1 (MQ=255)
                                                                                     aaCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCACGCGCGTCTCAGCgg                                                                  >  1:1703493/1‑150 (MQ=255)
                                                                                     aaCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCgg                                                                  >  2:1487993/1‑150 (MQ=255)
                                                                                       ccGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgag                                                                >  1:1381555/1‑150 (MQ=255)
                                                                                        cGGTAACCGAAATTACTTACACATGTAAATTCACTTTCCTTTAAAAAACAAAACTCCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:2092166/150‑1 (MQ=39)
                                                                                        cGGTAAATGTTATTACTTAAACATGTAAAGTCACTTTCCTTTAAAATAAAAAAATCCGCCAAAATCTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:2092155/150‑1 (MQ=38)
                                                                                        cGGTAAAAGATATTACTTAAACATGTCAATTCACTTTCCTTTAAAAAACAAAACACCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  1:1255439/150‑1 (MQ=255)
                                                                                        cGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  1:826866/150‑1 (MQ=255)
                                                                                        cGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:1593857/150‑1 (MQ=255)
                                                                                        cGGGAAACGTTATTACTTACACATGTAAATTCACTGTCCTGTAAAATACAAACATCCGCCACAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:2092150/150‑1 (MQ=38)
                                                                                                gATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTc                                                       <  2:1916321/150‑1 (MQ=255)
                                                                                                gATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTc                                                       <  1:1128918/150‑1 (MQ=255)
                                                                                                gATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTc                                                       <  1:229805/150‑1 (MQ=255)
                                                                                                   atTACTTAAACATGTAAATTCACTTTCCTTTAAAATACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCGGGTTCGGGGCCTTTCCAGCAGGTTGTATTAGCGTAGTAATGCTAGCGCGTCTCAGCGGAGACAATAGTCGcc                                                    >  2:139279/1‑150 (MQ=255)
                                                                                                   atTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGcc                                                    >  2:1208607/1‑150 (MQ=255)
                                                                                                    ttACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCa                                                   <  1:1006865/150‑1 (MQ=255)
                                                                                                    ttACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCa                                                   >  1:385445/1‑150 (MQ=255)
                                                                                                       cTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTGTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTa                                                >  2:1396594/1‑150 (MQ=255)
                                                                                                        ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                               >  1:95786/1‑150 (MQ=255)
                                                                                                        ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                               >  1:1187646/1‑150 (MQ=255)
                                                                                                        ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                               >  1:1131102/1‑150 (MQ=255)
                                                                                                                gTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttt                                       >  2:1013647/1‑150 (MQ=255)
                                                                                                                  aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                     >  2:209525/1‑150 (MQ=255)
                                                                                                                  aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                     <  1:1815781/150‑1 (MQ=255)
                                                                                                                  aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGCAGACAATACTCGCCAGTAACTCTCttttt                                     >  2:1626501/1‑150 (MQ=255)
                                                                                                                     ttCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTc                                  <  2:1263388/150‑1 (MQ=255)
                                                                                                                      tCACTTTACTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCa                                 <  1:1900056/150‑1 (MQ=255)
                                                                                                                      tCACTATCCATTCAAAAACACAAAACCGCCAAACTCAGGCGGTTTTATGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCa                                 <  2:1493946/150‑1 (MQ=39)
                                                                                                                            tCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaa                           <  1:2387815/150‑1 (MQ=255)
                                                                                                                            tCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaa                           <  1:2263227/150‑1 (MQ=255)
                                                                                                                             ccTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCaaaa                          >  1:1059501/1‑150 (MQ=255)
                                                                                                                                 tAAAAAACAAAAAACCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAagaga                      <  2:816270/150‑1 (MQ=255)
                                                                                                                                    aaaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTCTCAAGCAAAAGAGAGtt                   <  1:2181143/150‑1 (MQ=255)
                                                                                                                                     aaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGtta                  >  2:1273444/1‑150 (MQ=255)
                                                                                                                                        caaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttatta               <  1:2271467/150‑1 (MQ=255)
                                                                                                                                         aaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCCGCAGGTTGTATTACCGTAGTAATGCAAGCCCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTtattat              >  1:738475/1‑150 (MQ=255)
                                                                                                                                         aaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTAGCGGCCTTTCCAGCAGGTTTTATTAACGTAGGAATGCAAGCGCGGCAAAGCGGAGACAAAAAAAGCCACTAAATCTCTTTTTGTCAATCAAAAGAGAGTtattat              >  1:2077578/1‑150 (MQ=255)
                                                                                                                                           acccccGCCCAAATCCGGCGGTATTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCCGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg            <  1:567364/146‑1 (MQ=38)
                                                                                                                                           aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg            >  2:2228124/1‑150 (MQ=25)
                                                                                                                                           aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg            >  2:697628/1‑150 (MQ=25)
                                                                                                                                            aaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGt           <  1:1208607/150‑1 (MQ=255)
                                                                                                                                            aaacCGACAAAAACAGGCGGTATTTTGTTGCTGGTCCGGTTCTCGGCCTTTCCAGCATGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCCGAGACAATACTCCCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGt           <  1:139279/150‑1 (MQ=37)
                                                                                                                                              acCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTc         >  2:1361128/1‑150 (MQ=255)
                                                                                                                                              acCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTAACGTAGTAATTCAAGCGCGTCTCAGCGGAGACAATAATCGCCAGTAACTCTCTTTTTTTCAAGCAAAAGAGAGTTATTATttttg         >  1:1863546/1‑149 (MQ=38)
                                                                                                                                                cGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTATg       >  1:1738656/1‑150 (MQ=255)
                                                                                                                                                cGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGGTCGCGGCCTTTCCAGCAGGTTGTATTAACGTAGTAATTCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAATAGAGGTATTATttttgtt       >  1:2022675/1‑147 (MQ=38)
                                                                                                                                                 gCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGt      >  2:1423854/1‑150 (MQ=255)
                                                                                                                                                    aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg   >  2:1485071/1‑150 (MQ=255)
                                                                                                                                                    aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg   >  2:1783365/1‑150 (MQ=255)
                                                                                                                                                     aaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTAGTGTTCTGTTAgt  >  2:1267415/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCCGTGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGT  >  NC_000913/2653544‑2653842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: