Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,695 A→T 6.0% intergenic (+356/‑160) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,6950AT 6.0% 377.7 / 10.4 118intergenic (+356/‑160)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base A (49/58);  new base T (3/4);  total (52/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 124 total aligned reads displayed.

CCGTGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGT  >  NC_000913/2653546‑2653842
                                                                                                                                                     |                                                                                                                                                   
ccgTGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCaaaa                                                                                                                                                     <  2:393145/150‑1 (MQ=255)
    gtTCTGTATTAAAATATCCTGGCTTATTATTCCGAATTCAGAAAAATATAAATAAATCCTGCTCAAAATTAAAATTTCTAACCGTTAAAATATTTTAATTAACCATGTAAATTCACTGTCCTTTAAAAAACAAAAAACCGCCAAAATCAg                                                                                                                                                 <  1:1813196/150‑1 (MQ=255)
     ttCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAgg                                                                                                                                                <  1:1455879/150‑1 (MQ=255)
        ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                             >  2:836569/1‑150 (MQ=255)
        ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                             >  2:2330574/1‑150 (MQ=255)
        ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                             >  1:573703/1‑150 (MQ=255)
         gTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGt                                                                                                                                            >  1:2340164/1‑150 (MQ=255)
           aataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAACCAAAAAACCGCCAAAATCAGGCGGttt                                                                                                                                          <  1:1811636/150‑1 (MQ=255)
           aataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttt                                                                                                                                          <  2:1441773/150‑1 (MQ=255)
           aataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttt                                                                                                                                          <  2:1210428/150‑1 (MQ=255)
             taaaataaCCTGTATTATTAGTCATAATTCATAAAAATATAAATAAATCCTGATAAAAATTAAAAATTCTAACCGGTAAAAGATATTAATTAAACTTTTAAATTCACTTTCATTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttttt                                                                                                                                        <  1:1022243/150‑1 (MQ=255)
             taaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAACCCGCCTGATTTTGGCGGttttt                                                                                                                                        <  2:2126079/150‑1 (MQ=255)
              aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  2:275118/150‑1 (MQ=255)
              aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       >  1:1263388/1‑150 (MQ=255)
              aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  1:1674144/150‑1 (MQ=255)
              aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATAAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  1:837633/150‑1 (MQ=255)
              aaaataaCCTGGATTATTAGTCCGAATTCAGAAAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  1:169922/150‑1 (MQ=255)
                aataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgt                                                                                                                                     >  2:1006865/1‑150 (MQ=255)
                aataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgt                                                                                                                                     >  2:1487613/1‑150 (MQ=255)
                 ataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgtt                                                                                                                                    <  1:763057/150‑1 (MQ=255)
                   aaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                  <  1:1234809/150‑3 (MQ=39)
                   aaCCTGGCTTATTAGTCCGAATACAGAAAAATATAAATAAACCCGCCACAAAATAAAAAATTCTAACCGCTAAAAGATATTACTTAACCATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                  <  1:1903852/150‑3 (MQ=37)
                   aaCCTGCATTATTTGACCCAATACAGACAAATATAACAAAACCCCCCAAAAAAAAAAAATTTCTACCCGCTAAAATATATAACTAAAACATGAAAATACACTTTCCTTTAAAAAACAAAACACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                  <  1:1812243/150‑3 (MQ=255)
                      cTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAACGTTATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTgg                                                                                                                               <  1:374826/150‑1 (MQ=255)
                        ggCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTc                                                                                                                             >  1:912078/1‑150 (MQ=255)
                             attaGTCCGACTTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTTGTCCGGtt                                                                                                                        >  2:1017881/1‑150 (MQ=255)
                                  tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  2:1952593/1‑150 (MQ=255)
                                  tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  2:1815781/1‑150 (MQ=255)
                                  tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  1:1704493/1‑150 (MQ=255)
                                  tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  2:759430/1‑150 (MQ=255)
                                           aGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCcag                                                                                                          >  2:593978/1‑150 (MQ=255)
                                               aaaTATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAgg                                                                                                      >  2:2217191/1‑150 (MQ=255)
                                                 atatAAATTAAGCCTGCTCAACATTAAAAATTCTACCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCACAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                    <  2:701139/150‑1 (MQ=39)
                                                 atatAAATAAATCCTGCACAAACTTACAAATTCTACCCGGTTAACGTTATGACTTACACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                    <  2:1026898/150‑1 (MQ=38)
                                                    taaataaaTCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTa                                                                                                 <  2:907120/150‑1 (MQ=255)
                                                        taaatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAAAAAAAAAACGCCAAAATCAGGAGGTTTTTTTTTGCTGGTCCGGTTCGCGGCCTTTCCAGAAGTGTGTATTAc                                                                                             >  2:880024/1‑150 (MQ=255)
                                                         aaatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTAcc                                                                                            <  1:1641089/150‑1 (MQ=255)
                                                         aaatCCTGATAAAAATTAAAAATTCTTACCGTTAAAAGATATTACTTAAACATGTATATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCAGGTTCGCGGCCTTTCCAGCAGGTTGTATTAcc                                                                                            <  1:1641183/150‑1 (MQ=38)
                                                          aatCCTGCTCAAAATTAAAAATTCTAACCGGTACAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAACAACAAAACACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCg                                                                                           <  1:1538792/150‑1 (MQ=255)
                                                                 cTCAAAATTAAAAATTCTAACCGGTACAAGATATTACTTAAACATGTAAATTCACTGTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                    <  1:811987/150‑1 (MQ=255)
                                                                 cTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                    <  1:2282617/150‑1 (MQ=255)
                                                                 cTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                    <  2:1357709/150‑1 (MQ=255)
                                                                    aaaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCa                                                                                 >  2:842339/1‑150 (MQ=255)
                                                                    aaaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCa                                                                                 <  2:1214942/150‑1 (MQ=255)
                                                                      aaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAg                                                                               >  1:98077/1‑150 (MQ=255)
                                                                            aaaTTCTAACCGGTAAACGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:719472/150‑1 (MQ=255)
                                                                            aaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAACACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:80682/150‑1 (MQ=255)
                                                                            aaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:1850133/150‑1 (MQ=255)
                                                                              aTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtctc                                                                       >  2:1745439/1‑150 (MQ=255)
                                                                                  tAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCg                                                                   <  1:870833/150‑1 (MQ=255)
                                                                                   aaCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCACGCGCGTCTCAGCgg                                                                  >  1:1703493/1‑150 (MQ=255)
                                                                                   aaCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCgg                                                                  >  2:1487993/1‑150 (MQ=255)
                                                                                     ccGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgag                                                                >  1:1381555/1‑150 (MQ=255)
                                                                                      cGGTAACCGAAATTACTTACACATGTAAATTCACTTTCCTTTAAAAAACAAAACTCCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:2092166/150‑1 (MQ=39)
                                                                                      cGGTAAATGTTATTACTTAAACATGTAAAGTCACTTTCCTTTAAAATAAAAAAATCCGCCAAAATCTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:2092155/150‑1 (MQ=38)
                                                                                      cGGTAAAAGATATTACTTAAACATGTCAATTCACTTTCCTTTAAAAAACAAAACACCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  1:1255439/150‑1 (MQ=255)
                                                                                      cGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:1593857/150‑1 (MQ=255)
                                                                                      cGGGAAACGTTATTACTTACACATGTAAATTCACTGTCCTGTAAAATACAAACATCCGCCACAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:2092150/150‑1 (MQ=38)
                                                                                              gATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTc                                                       <  2:1916321/150‑1 (MQ=255)
                                                                                              gATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTc                                                       <  1:1128918/150‑1 (MQ=255)
                                                                                                 atTACTTAAACATGTAAATTCACTTTCCTTTAAAATACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCGGGTTCGGGGCCTTTCCAGCAGGTTGTATTAGCGTAGTAATGCTAGCGCGTCTCAGCGGAGACAATAGTCGcc                                                    >  2:139279/1‑150 (MQ=255)
                                                                                                  ttACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCa                                                   >  1:232583/1‑150 (MQ=255)
                                                                                                  ttACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCa                                                   <  1:1006865/150‑1 (MQ=255)
                                                                                                    aCTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGt                                                 >  2:1502002/1‑150 (MQ=255)
                                                                                                     cTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTa                                                >  2:2040305/1‑150 (MQ=255)
                                                                                                     cTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTGTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTa                                                >  2:1396594/1‑150 (MQ=255)
                                                                                                      ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                               >  1:1131102/1‑150 (MQ=255)
                                                                                                      ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                               >  1:95786/1‑150 (MQ=255)
                                                                                                      ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                               >  1:1187646/1‑150 (MQ=255)
                                                                                                              gTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttt                                       >  2:1013647/1‑150 (MQ=255)
                                                                                                                aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                     <  1:759430/150‑1 (MQ=255)
                                                                                                                aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                     <  1:1815781/150‑1 (MQ=255)
                                                                                                                aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                     <  1:1952593/150‑1 (MQ=255)
                                                                                                                aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGCAGACAATACTCGCCAGTAACTCTCttttt                                     >  2:1626501/1‑150 (MQ=255)
                                                                                                                   ttCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTc                                  <  2:1263388/150‑1 (MQ=255)
                                                                                                                    tCACTTTACTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCa                                 <  1:1900056/150‑1 (MQ=255)
                                                                                                                          tCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaa                           <  1:2263227/150‑1 (MQ=255)
                                                                                                                          tCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaa                           <  1:2263215/150‑1 (MQ=255)
                                                                                                                          tCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaa                           <  1:2387815/150‑1 (MQ=255)
                                                                                                                           ccTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCaaaa                          >  1:1059501/1‑150 (MQ=255)
                                                                                                                               tAAAAAACAAAAAACCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAagaga                      <  2:816270/150‑1 (MQ=255)
                                                                                                                                  aaaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTCTCAAGCAAAAGAGAGtt                   <  1:2181143/150‑1 (MQ=255)
                                                                                                                                      caaaacacCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttatta               <  1:552411/150‑1 (MQ=255)
                                                                                                                                      caaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttatta               <  1:2271467/150‑1 (MQ=255)
                                                                                                                                       aaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTAGCGGCCTTTCCAGCAGGTTTTATTAACGTAGGAATGCAAGCGCGGCAAAGCGGAGACAAAAAAAGCCACTAAATCTCTTTTTGTCAATCAAAAGAGAGTtattat              >  1:2077578/1‑150 (MQ=255)
                                                                                                                                         acccccGCCCAAATCCGGCGGTATTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCCGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg            <  1:567364/146‑1 (MQ=38)
                                                                                                                                         aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg            >  2:2228124/1‑150 (MQ=25)
                                                                                                                                         aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg            >  2:2112143/1‑150 (MQ=25)
                                                                                                                                         aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg            >  2:697628/1‑150 (MQ=25)
                                                                                                                                          aaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGt           <  1:1208607/150‑1 (MQ=255)
                                                                                                                                          aaacCGACAAAAACAGGCGGTATTTTGTTGCTGGTCCGGTTCTCGGCCTTTCCAGCATGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCCGAGACAATACTCCCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGt           <  1:139279/150‑1 (MQ=37)
                                                                                                                                            acCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTc         >  2:1361128/1‑150 (MQ=255)
                                                                                                                                            acCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTAACGTAGTAATTCAAGCGCGTCTCAGCGGAGACAATAATCGCCAGTAACTCTCTTTTTTTCAAGCAAAAGAGAGTTATTATttttg         >  1:1863546/1‑149 (MQ=38)
                                                                                                                                              cGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTATg       >  1:1738656/1‑150 (MQ=255)
                                                                                                                                              cGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGGTCGCGGCCTTTCCAGCAGGTTGTATTAACGTAGTAATTCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAATAGAGGTATTATttttgtt       >  1:2022675/1‑147 (MQ=38)
                                                                                                                                                  aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg   >  2:1783365/1‑150 (MQ=255)
                                                                                                                                                  aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg   >  2:1485071/1‑150 (MQ=255)
                                                                                                                                                  aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg   >  2:1783270/1‑150 (MQ=255)
                                                                                                                                                   ataTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCATGCGCGTATCATCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAATAGAGTTATTATTGTTCTGTTAgt  <  1:1898414/148‑1 (MQ=255)
                                                                                                                                                   aaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTAGTGTTCTGTTAgt  >  2:1267415/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCGTGTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGT  >  NC_000913/2653546‑2653842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: