Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,697 C→T 5.7% intergenic (+358/‑158) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,6970CT 5.7% 418.7 / 10.3 123intergenic (+358/‑158)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base C (53/63);  new base T (3/4);  total (56/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

Only 100 of 123 total aligned reads displayed.

GTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGT  >  NC_000913/2653550‑2653842
                                                                                                                                                   |                                                                                                                                                 
gtTCTGTATTAAAATATCCTGGCTTATTATTCCGAATTCAGAAAAATATAAATAAATCCTGCTCAAAATTAAAATTTCTAACCGTTAAAATATTTTAATTAACCATGTAAATTCACTGTCCTTTAAAAAACAAAAAACCGCCAAAATCAg                                                                                                                                                 <  1:1813196/150‑1 (MQ=255)
 ttCGTTAATAAAATAACCTGGATTATTTGTCCTAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAgg                                                                                                                                                <  1:1500728/150‑1 (MQ=255)
 ttCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAgg                                                                                                                                                <  1:1455879/150‑1 (MQ=255)
 ttCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCATGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAgg                                                                                                                                                <  2:1955944/150‑1 (MQ=255)
    ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                             >  2:836569/1‑150 (MQ=255)
    ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                             >  1:573703/1‑150 (MQ=255)
    ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                             >  2:2330574/1‑150 (MQ=255)
       attaaaataaCCTGTCTTATAAGTCCGAATTCATAAAATTATAAATAAATCCTGCACAAAAAAAAAAATTCTACCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttt                                                                                                                                          <  1:1811631/148‑1 (MQ=255)
       aataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttt                                                                                                                                          <  2:1441773/150‑1 (MQ=255)
       aataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttt                                                                                                                                          <  2:1210428/150‑1 (MQ=255)
         taaaataaCCTGTATTATTAGTCATAATTCATAAAAATATAAATAAATCCTGATAAAAATTAAAAATTCTAACCGGTAAAAGATATTAATTAAACTTTTAAATTCACTTTCATTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttttt                                                                                                                                        <  1:1022243/150‑1 (MQ=255)
         taaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAACCCGCCTGATTTTGGCGGttttt                                                                                                                                        <  2:2126079/150‑1 (MQ=255)
          aaaataaCCTGGCTTATTAGTCCTAATTCACACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGTTAAAAGATATTACTTAAACATTTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  1:1674462/150‑1 (MQ=255)
          aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       >  1:1263388/1‑150 (MQ=255)
          aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  2:275118/150‑1 (MQ=255)
          aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  1:1674144/150‑1 (MQ=255)
          aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATAAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                       <  1:837633/150‑1 (MQ=255)
            aataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgt                                                                                                                                     >  2:1006865/1‑150 (MQ=255)
               aaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                  <  1:1234809/150‑3 (MQ=39)
               aaCCTGGCTTATTAGTCCGAATACAGAAAAATATAAATAAACCCGCCACAAAATAAAAAATTCTAACCGCTAAAAGATATTACTTAACCATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                  <  1:1903852/150‑3 (MQ=37)
               aaCCTGCATTATTTGACCCAATACAGACAAATATAACAAAACCCCCCAAAAAAAAAAAATTTCTACCCGCTAAAATATATAACTAAAACATGAAAATACACTTTCCTTTAAAAAACAAAACACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                  <  1:1812243/150‑3 (MQ=255)
                  cTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAACGTTATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTgg                                                                                                                               <  1:374826/150‑1 (MQ=255)
                         attaGTCCGACTTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTTGTCCGGtt                                                                                                                        >  2:1017881/1‑150 (MQ=255)
                              tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  1:1704493/1‑150 (MQ=255)
                              tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  2:1815781/1‑150 (MQ=255)
                              tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  2:1952593/1‑150 (MQ=255)
                              tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                   >  2:759430/1‑150 (MQ=255)
                                       aGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCcag                                                                                                          >  2:593978/1‑150 (MQ=255)
                                             atatAAATTAAGCCTGCTCAACATTAAAAATTCTACCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCACAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                    <  2:701139/150‑1 (MQ=39)
                                             atatAAATAAATCCTGCACAAACTTACAAATTCTACCCGGTTAACGTTATGACTTACACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                    <  2:1026898/150‑1 (MQ=38)
                                                taaataaaTCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTa                                                                                                 <  2:907120/150‑1 (MQ=255)
                                                    taaatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAAAAAAAAAACGCCAAAATCAGGAGGTTTTTTTTTGCTGGTCCGGTTCGCGGCCTTTCCAGAAGTGTGTATTAc                                                                                             >  2:880024/1‑150 (MQ=255)
                                                     aaatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTAcc                                                                                            <  1:1641089/150‑1 (MQ=255)
                                                     aaatCCTGATAAAAATTAAAAATTCTTACCGTTAAAAGATATTACTTAAACATGTATATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCAGGTTCGCGGCCTTTCCAGCAGGTTGTATTAcc                                                                                            <  1:1641183/150‑1 (MQ=38)
                                                           tGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGATGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACAgtagta                                                                                      >  1:2215592/1‑150 (MQ=255)
                                                             cTCAAAATTAAAAATTCTAACCGGTACAAGATATTACTTAAACATGTAAATTCACTGTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                    <  1:811987/150‑1 (MQ=255)
                                                             cTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                    <  2:1357709/150‑1 (MQ=255)
                                                             cTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                    <  1:2282617/150‑1 (MQ=255)
                                                                aaaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCa                                                                                 >  2:842339/1‑150 (MQ=255)
                                                                aaaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCa                                                                                 >  1:998704/1‑150 (MQ=255)
                                                                  aaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAg                                                                               >  1:98077/1‑150 (MQ=255)
                                                                     tAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAgcgc                                                                            <  2:1749012/150‑1 (MQ=255)
                                                                       aaaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGt                                                                          >  2:2258438/1‑150 (MQ=255)
                                                                        aaaTTCTAACCGGTAAACGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:719472/150‑1 (MQ=255)
                                                                        aaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAACACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:80682/150‑1 (MQ=255)
                                                                        aaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:1917478/150‑1 (MQ=255)
                                                                        aaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                         <  1:1850133/150‑1 (MQ=255)
                                                                          aTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtctc                                                                       >  2:1745439/1‑150 (MQ=255)
                                                                              tAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCg                                                                   <  1:870833/150‑1 (MQ=255)
                                                                               aaCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCACGCGCGTCTCAGCgg                                                                  >  1:1703493/1‑150 (MQ=255)
                                                                               aaCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCgg                                                                  >  2:1487993/1‑150 (MQ=255)
                                                                                 ccGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgag                                                                >  1:1381555/1‑150 (MQ=255)
                                                                                  cGGTAACCGAAATTACTTACACATGTAAATTCACTTTCCTTTAAAAAACAAAACTCCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:2092166/150‑1 (MQ=39)
                                                                                  cGGTAAAAGATATTACTTAAACATGTCAATTCACTTTCCTTTAAAAAACAAAACACCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  1:1255439/150‑1 (MQ=255)
                                                                                  cGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  1:826866/150‑1 (MQ=255)
                                                                                  cGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:1593857/150‑1 (MQ=255)
                                                                                  cGGGAAACGTTATTACTTACACATGTAAATTCACTGTCCTGTAAAATACAAACATCCGCCACAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                               <  2:2092150/150‑1 (MQ=38)
                                                                                          gATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTc                                                       <  1:229805/150‑1 (MQ=255)
                                                                                          gATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTc                                                       <  1:1128918/150‑1 (MQ=255)
                                                                                             atTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGcc                                                    >  2:1208607/1‑150 (MQ=255)
                                                                                              ttACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCa                                                   <  1:1006865/150‑1 (MQ=255)
                                                                                              ttACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCa                                                   >  1:232583/1‑150 (MQ=255)
                                                                                              ttACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCa                                                   >  1:385445/1‑150 (MQ=255)
                                                                                                aCTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGt                                                 >  2:1502002/1‑150 (MQ=255)
                                                                                                 cTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTa                                                >  2:2040305/1‑150 (MQ=255)
                                                                                                 cTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTGTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTa                                                >  2:1396594/1‑150 (MQ=255)
                                                                                                  ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                               >  1:95786/1‑150 (MQ=255)
                                                                                                  ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                               >  1:1131102/1‑150 (MQ=255)
                                                                                                  ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                               >  1:1187646/1‑150 (MQ=255)
                                                                                                          gTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttt                                       <  1:1839410/150‑1 (MQ=255)
                                                                                                            aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                     >  2:209525/1‑150 (MQ=255)
                                                                                                            aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                     <  1:1815781/150‑1 (MQ=255)
                                                                                                            aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                     <  1:759430/150‑1 (MQ=255)
                                                                                                               ttCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTc                                  <  2:1263388/150‑1 (MQ=255)
                                                                                                                tCACTTTACTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCa                                 <  1:1900056/150‑1 (MQ=255)
                                                                                                                tCACTATCCATTCAAAAACACAAAACCGCCAAACTCAGGCGGTTTTATGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCa                                 <  2:1493946/150‑1 (MQ=39)
                                                                                                                      tCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaa                           <  1:2387815/150‑1 (MQ=255)
                                                                                                                      tCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaa                           <  1:2263227/150‑1 (MQ=255)
                                                                                                                       ccTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCaaaa                          >  1:1059501/1‑150 (MQ=255)
                                                                                                                           tAAAAAACAAAAAACCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAagaga                      <  2:816270/150‑1 (MQ=255)
                                                                                                                              aaaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTCTCAAGCAAAAGAGAGtt                   <  1:2181143/150‑1 (MQ=255)
                                                                                                                               aaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGtta                  >  2:1273444/1‑150 (MQ=255)
                                                                                                                                  caaaacacCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttatta               <  1:552411/150‑1 (MQ=255)
                                                                                                                                  caaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttatta               <  1:2271467/150‑1 (MQ=255)
                                                                                                                                   aaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCCGCAGGTTGTATTACCGTAGTAATGCAAGCCCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTtattat              >  1:738475/1‑150 (MQ=255)
                                                                                                                                   aaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTAGCGGCCTTTCCAGCAGGTTTTATTAACGTAGGAATGCAAGCGCGGCAAAGCGGAGACAAAAAAAGCCACTAAATCTCTTTTTGTCAATCAAAAGAGAGTtattat              >  1:2077578/1‑150 (MQ=255)
                                                                                                                                     aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg            >  2:2112143/1‑150 (MQ=25)
                                                                                                                                     aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg            >  2:2228124/1‑150 (MQ=25)
                                                                                                                                     aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg            >  2:697628/1‑150 (MQ=25)
                                                                                                                                      aaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGt           <  1:1208607/150‑1 (MQ=255)
                                                                                                                                      aaacCGACAAAAACAGGCGGTATTTTGTTGCTGGTCCGGTTCTCGGCCTTTCCAGCATGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCCGAGACAATACTCCCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGt           <  1:139279/150‑1 (MQ=37)
                                                                                                                                        acCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTc         >  2:1361128/1‑150 (MQ=255)
                                                                                                                                          cGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTATg       >  1:1738656/1‑150 (MQ=255)
                                                                                                                                          cGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGGTCGCGGCCTTTCCAGCAGGTTGTATTAACGTAGTAATTCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAATAGAGGTATTATttttgtt       >  1:2022675/1‑147 (MQ=38)
                                                                                                                                           gCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGt      >  2:1423854/1‑150 (MQ=255)
                                                                                                                                              aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg   >  2:1783365/1‑150 (MQ=255)
                                                                                                                                              aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg   >  2:1783270/1‑150 (MQ=255)
                                                                                                                                              aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg   >  2:1485071/1‑150 (MQ=255)
                                                                                                                                               ataTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCATGCGCGTATCATCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAATAGAGTTATTATTGTTCTGTTAgt  <  1:1898414/148‑1 (MQ=255)
                                                                                                                                               aaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTAGTGTTCTGTTAgt  >  2:1267415/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGT  >  NC_000913/2653550‑2653842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: