Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,698 A→T 6.2% intergenic (+359/‑157) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,6980AT 6.2% 405.7 / 13.4 123intergenic (+359/‑157)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base A (53/61);  new base T (3/5);  total (56/67)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 124 total aligned reads displayed.

GTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGTGTATT  >  NC_000913/2653550‑2653847
                                                                                                                                                    |                                                                                                                                                     
gtTCTGTATTAAAATATCCTGGCTTATTATTCCGAATTCAGAAAAATATAAATAAATCCTGCTCAAAATTAAAATTTCTAACCGTTAAAATATTTTAATTAACCATGTAAATTCACTGTCCTTTAAAAAACAAAAAACCGCCAAAATCAg                                                                                                                                                      <  1:1813196/150‑1 (MQ=255)
 ttCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAgg                                                                                                                                                     <  1:1455879/150‑1 (MQ=255)
    ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                                  >  2:836569/1‑150 (MQ=255)
    ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                                  >  2:2330574/1‑150 (MQ=255)
    ggTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCgg                                                                                                                                                  >  1:573703/1‑150 (MQ=255)
     gTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGt                                                                                                                                                 >  1:2340164/1‑150 (MQ=255)
       aataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAACCAAAAAACCGCCAAAATCAGGCGGttt                                                                                                                                               <  1:1811636/150‑1 (MQ=255)
       aataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttt                                                                                                                                               <  2:1441773/150‑1 (MQ=255)
       aataaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttt                                                                                                                                               <  2:1210428/150‑1 (MQ=255)
         taaaataaCCTGTATTATTAGTCATAATTCATAAAAATATAAATAAATCCTGATAAAAATTAAAAATTCTAACCGGTAAAAGATATTAATTAAACTTTTAAATTCACTTTCATTTAAAAAACAAAAAACCGCCAAAATCAGGCGGttttt                                                                                                                                             <  1:1022243/150‑1 (MQ=255)
         taaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAACCCGCCTGATTTTGGCGGttttt                                                                                                                                             <  2:2126079/150‑1 (MQ=255)
          aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                            <  2:275118/150‑1 (MQ=255)
          aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                            >  1:1263388/1‑150 (MQ=255)
          aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                            <  1:1674144/150‑1 (MQ=255)
          aaaataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATAAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                            <  1:837633/150‑1 (MQ=255)
          aaaataaCCTGGATTATTAGTCCGAATTCAGAAAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGtttttt                                                                                                                                            <  1:169922/150‑1 (MQ=255)
            aataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgt                                                                                                                                          >  2:1006865/1‑150 (MQ=255)
            aataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgt                                                                                                                                          >  2:1487613/1‑150 (MQ=255)
             ataaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTttgtt                                                                                                                                         <  1:763057/150‑1 (MQ=255)
               aaCCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                       <  1:1234809/150‑3 (MQ=39)
               aaCCTGGCTTATTAGTCCGAATACAGAAAAATATAAATAAACCCGCCACAAAATAAAAAATTCTAACCGCTAAAAGATATTACTTAACCATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                       <  1:1903852/150‑3 (MQ=37)
               aaCCTGCATTATTTGACCCAATACAGACAAATATAACAAAACCCCCCAAAAAAAAAAAATTTCTACCCGCTAAAATATATAACTAAAACATGAAAATACACTTTCCTTTAAAAAACAAAACACCGCCTGATTTTGGCGGTTTTttgtttt                                                                                                                                       <  1:1812243/150‑3 (MQ=255)
                  cTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAACGTTATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTgg                                                                                                                                    <  1:374826/150‑1 (MQ=255)
                    ggCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTc                                                                                                                                  >  1:912078/1‑150 (MQ=255)
                         attaGTCCGACTTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTTGTCCGGtt                                                                                                                             >  2:1017881/1‑150 (MQ=255)
                              tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                        >  2:1815781/1‑150 (MQ=255)
                              tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                        >  2:1952593/1‑150 (MQ=255)
                              tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                        >  1:1704493/1‑150 (MQ=255)
                              tCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCgg                                                                                                                        >  2:759430/1‑150 (MQ=255)
                                       aGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCcag                                                                                                               >  2:593978/1‑150 (MQ=255)
                                           aaaTATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAgg                                                                                                           >  2:2217191/1‑150 (MQ=255)
                                             atatAAATTAAGCCTGCTCAACATTAAAAATTCTACCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCACAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                         <  2:701139/150‑1 (MQ=39)
                                             atatAAATAAATCCTGCACAAACTTACAAATTCTACCCGGTTAACGTTATGACTTACACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                         <  2:1026898/150‑1 (MQ=38)
                                                taaataaaTCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTa                                                                                                      <  2:907120/150‑1 (MQ=255)
                                                    taaatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAAAAAAAAAACGCCAAAATCAGGAGGTTTTTTTTTGCTGGTCCGGTTCGCGGCCTTTCCAGAAGTGTGTATTAc                                                                                                  >  2:880024/1‑150 (MQ=255)
                                                     aaatCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTAcc                                                                                                 <  1:1641089/150‑1 (MQ=255)
                                                     aaatCCTGATAAAAATTAAAAATTCTTACCGTTAAAAGATATTACTTAAACATGTATATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCAGGTTCGCGGCCTTTCCAGCAGGTTGTATTAcc                                                                                                 <  1:1641183/150‑1 (MQ=38)
                                                      aatCCTGCTCAAAATTAAAAATTCTAACCGGTACAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAACAACAAAACACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCg                                                                                                <  1:1538792/150‑1 (MQ=255)
                                                             cTCAAAATTAAAAATTCTAACCGGTACAAGATATTACTTAAACATGTAAATTCACTGTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                         <  1:811987/150‑1 (MQ=255)
                                                             cTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                         <  2:1357709/150‑1 (MQ=255)
                                                             cTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAAt                                                                                         <  1:2282617/150‑1 (MQ=255)
                                                                aaaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCa                                                                                      >  2:842339/1‑150 (MQ=255)
                                                                aaaaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCa                                                                                      <  2:1214942/150‑1 (MQ=255)
                                                                  aaTTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAg                                                                                    >  1:98077/1‑150 (MQ=255)
                                                                        aaaTTCTAACCGGTAAACGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                              <  1:719472/150‑1 (MQ=255)
                                                                        aaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAACACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                              <  1:80682/150‑1 (MQ=255)
                                                                        aaaTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtc                                                                              <  1:1850133/150‑1 (MQ=255)
                                                                          aTTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGtctc                                                                            >  2:1745439/1‑150 (MQ=255)
                                                                              tAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCg                                                                        <  1:870833/150‑1 (MQ=255)
                                                                               aaCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCACGCGCGTCTCAGCgg                                                                       >  1:1703493/1‑150 (MQ=255)
                                                                               aaCCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCgg                                                                       >  2:1487993/1‑150 (MQ=255)
                                                                                 ccGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgag                                                                     >  1:1381555/1‑150 (MQ=255)
                                                                                  cGGTAACCGAAATTACTTACACATGTAAATTCACTTTCCTTTAAAAAACAAAACTCCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                                    <  2:2092166/150‑1 (MQ=39)
                                                                                  cGGTAAATGTTATTACTTAAACATGTAAAGTCACTTTCCTTTAAAATAAAAAAATCCGCCAAAATCTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                                    <  2:2092155/150‑1 (MQ=38)
                                                                                  cGGTAAAAGATATTACTTAAACATGTCAATTCACTTTCCTTTAAAAAACAAAACACCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                                    <  1:1255439/150‑1 (MQ=255)
                                                                                  cGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                                    <  2:1593857/150‑1 (MQ=255)
                                                                                  cGGGAAACGTTATTACTTACACATGTAAATTCACTGTCCTGTAAAATACAAACATCCGCCACAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGgaga                                                                    <  2:2092150/150‑1 (MQ=38)
                                                                                          gATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTc                                                            <  2:1916321/150‑1 (MQ=255)
                                                                                          gATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTc                                                            <  1:1128918/150‑1 (MQ=255)
                                                                                             atTACTTAAACATGTAAATTCACTTTCCTTTAAAATACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCGGGTTCGGGGCCTTTCCAGCAGGTTGTATTAGCGTAGTAATGCTAGCGCGTCTCAGCGGAGACAATAGTCGcc                                                         >  2:139279/1‑150 (MQ=255)
                                                                                              ttACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCa                                                        >  1:232583/1‑150 (MQ=255)
                                                                                              ttACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCa                                                        <  1:1006865/150‑1 (MQ=255)
                                                                                                aCTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGt                                                      >  2:1502002/1‑150 (MQ=255)
                                                                                                 cTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTa                                                     >  2:2040305/1‑150 (MQ=255)
                                                                                                 cTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTGTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTa                                                     >  2:1396594/1‑150 (MQ=255)
                                                                                                  ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                                    >  1:1187646/1‑150 (MQ=255)
                                                                                                  ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                                    >  1:95786/1‑150 (MQ=255)
                                                                                                  ttAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTaa                                                    >  1:1131102/1‑150 (MQ=255)
                                                                                                          gTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttt                                            >  2:1013647/1‑150 (MQ=255)
                                                                                                            aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                          <  1:1815781/150‑1 (MQ=255)
                                                                                                            aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                          <  1:1952593/150‑1 (MQ=255)
                                                                                                            aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCttttt                                          <  1:759430/150‑1 (MQ=255)
                                                                                                            aaaTTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGCAGACAATACTCGCCAGTAACTCTCttttt                                          >  2:1626501/1‑150 (MQ=255)
                                                                                                               ttCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTc                                       <  2:1263388/150‑1 (MQ=255)
                                                                                                                tCACTTTACTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCa                                      <  1:1900056/150‑1 (MQ=255)
                                                                                                                      tCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaa                                <  1:2263227/150‑1 (MQ=255)
                                                                                                                      tCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaa                                <  1:2263215/150‑1 (MQ=255)
                                                                                                                      tCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCaaa                                <  1:2387815/150‑1 (MQ=255)
                                                                                                                       ccTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCaaaa                               >  1:1059501/1‑150 (MQ=255)
                                                                                                                           tAAAAAACAAAAAACCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAagaga                           <  2:816270/150‑1 (MQ=255)
                                                                                                                              aaaacaaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTCTCAAGCAAAAGAGAGtt                        <  1:2181143/150‑1 (MQ=255)
                                                                                                                                  caaaacacCGCCAAACTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttatta                    <  1:552411/150‑1 (MQ=255)
                                                                                                                                  caaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGttatta                    <  1:2271467/150‑1 (MQ=255)
                                                                                                                                   aaaaaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTAGCGGCCTTTCCAGCAGGTTTTATTAACGTAGGAATGCAAGCGCGGCAAAGCGGAGACAAAAAAAGCCACTAAATCTCTTTTTGTCAATCAAAAGAGAGTtattat                   >  1:2077578/1‑150 (MQ=255)
                                                                                                                                     acccccGCCCAAATCCGGCGGTATTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCCGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg                 <  1:567364/146‑1 (MQ=38)
                                                                                                                                     aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg                 >  2:2112143/1‑150 (MQ=25)
                                                                                                                                     aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg                 >  2:2228124/1‑150 (MQ=25)
                                                                                                                                     aaaacCGCCTGATTTTGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTg                 >  2:697628/1‑150 (MQ=25)
                                                                                                                                      aaacCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGt                <  1:1208607/150‑1 (MQ=255)
                                                                                                                                      aaacCGACAAAAACAGGCGGTATTTTGTTGCTGGTCCGGTTCTCGGCCTTTCCAGCATGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCCGAGACAATACTCCCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGt                <  1:139279/150‑1 (MQ=37)
                                                                                                                                        acCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTc              >  2:1361128/1‑150 (MQ=255)
                                                                                                                                        acCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTAACGTAGTAATTCAAGCGCGTCTCAGCGGAGACAATAATCGCCAGTAACTCTCTTTTTTTCAAGCAAAAGAGAGTTATTATttttg              >  1:1863546/1‑149 (MQ=38)
                                                                                                                                          cGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTATg            >  1:1738656/1‑150 (MQ=255)
                                                                                                                                          cGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGGTCGCGGCCTTTCCAGCAGGTTGTATTAACGTAGTAATTCAAGCGCGTCTCAGCGGAGACAAAACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAATAGAGGTATTATttttgtt            >  1:2022675/1‑147 (MQ=38)
                                                                                                                                              aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg        >  2:1783270/1‑150 (MQ=255)
                                                                                                                                              aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg        >  2:1485071/1‑150 (MQ=255)
                                                                                                                                              aaaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAg        >  2:1783365/1‑150 (MQ=255)
                                                                                                                                               ataTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCATGCGCGTATCATCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAATAGAGTTATTATTGTTCTGTTAgt       <  1:1898414/148‑1 (MQ=255)
                                                                                                                                               aaaTCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTAGTGTTCTGTTAgt       >  2:1267415/1‑150 (MQ=255)
                                                                                                                                                    aGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTATCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGTGtatt  <  2:2327221/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTTCGGTAATAAAATAACCTGGCTTATTAGTCCGAATTCAGACAAATATAAATAAATCCTGCTCAAAATTAAAAATTCTAACCGGTAAAAGATATTACTTAAACATGTAAATTCACTTTCCTTTAAAAAACAAAAAACCGCCAAAATCAGGCGGTTTTTTGTTGCTGGTCCGGTTCGCGGCCTTTCCAGCAGGTTGTATTACCGTAGTAATGCAAGCGCGTCTCAGCGGAGACAATACTCGCCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTTATTATTGTTCTGTTAGTGTATT  >  NC_000913/2653550‑2653847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: