Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,739,179 A→G 5.7% A297A (GCT→GCC tyrA ← fused chorismate mutase T/prephenate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,739,1790AG 5.7% 594.5 / 18.5 175A297A (GCT→GCCtyrAfused chorismate mutase T/prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (98/67);  new base G (5/5);  total (103/72)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 181 total aligned reads displayed.

TCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCTGAATAAACGCCATATT  >  NC_000913/2739030‑2739328
                                                                                                                                                     |                                                                                                                                                     
tCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGa                                                                                                                                                       <  1:67628/150‑1 (MQ=255)
 ccACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAg                                                                                                                                                      >  1:1157543/1‑150 (MQ=255)
 ccACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAg                                                                                                                                                      >  1:716050/1‑150 (MQ=255)
  cACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGc                                                                                                                                                     >  2:247457/1‑150 (MQ=255)
  cACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGc                                                                                                                                                     >  1:129201/1‑150 (MQ=255)
   aCCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCa                                                                                                                                                    >  1:582628/1‑150 (MQ=255)
    ccTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCaa                                                                                                                                                   >  1:2105654/1‑150 (MQ=255)
     cTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCaaa                                                                                                                                                  <  1:446499/150‑1 (MQ=255)
     cTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCaaa                                                                                                                                                  >  1:1794652/1‑150 (MQ=255)
     cTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCaaa                                                                                                                                                  >  1:1705899/1‑150 (MQ=255)
     cTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGAAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACTCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCaaa                                                                                                                                                  >  1:717703/1‑150 (MQ=255)
      ttGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAAc                                                                                                                                                 >  1:51696/1‑150 (MQ=255)
              aCTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGccc                                                                                                                                         <  1:1980371/150‑1 (MQ=255)
                  tCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGAcc                                                                                                                                     <  1:229419/150‑1 (MQ=255)
                     aTAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATc                                                                                                                                  >  2:164344/1‑150 (MQ=255)
                     aTAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATc                                                                                                                                  >  1:650423/1‑150 (MQ=255)
                     aTAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATc                                                                                                                                  >  2:1710046/1‑150 (MQ=255)
                     aTAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATc                                                                                                                                  >  1:1889821/1‑150 (MQ=255)
                          cGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGAAAACAGTCGCCCGACCATCTCCAg                                                                                                                             >  1:2240633/1‑150 (MQ=255)
                                cTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAg                                                                                                                       >  1:995015/1‑150 (MQ=255)
                                cTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAg                                                                                                                       <  1:1181090/150‑1 (MQ=255)
                                    tCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCgg                                                                                                                   >  2:360996/1‑150 (MQ=255)
                                    tCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCgg                                                                                                                   >  2:2088227/1‑150 (MQ=255)
                                         cTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAAt                                                                                                              <  2:129201/150‑1 (MQ=255)
                                          tGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATc                                                                                                             <  1:617004/150‑1 (MQ=255)
                                           gCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCg                                                                                                            >  1:857333/1‑150 (MQ=255)
                                            cTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCgg                                                                                                           >  1:557719/1‑150 (MQ=255)
                                            cTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCgg                                                                                                           >  1:152458/1‑150 (MQ=255)
                                            cTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCgg                                                                                                           >  1:484091/1‑150 (MQ=255)
                                              ccAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCg                                                                                                         >  1:1837489/1‑150 (MQ=255)
                                              ccAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCg                                                                                                         >  1:2106041/1‑150 (MQ=255)
                                               cAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGa                                                                                                        >  2:1204646/1‑150 (MQ=255)
                                                aGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGGGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGaa                                                                                                       >  2:1328608/1‑150 (MQ=255)
                                                aGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGGGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGaa                                                                                                       >  2:1328516/1‑150 (MQ=255)
                                                aGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGGGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGaa                                                                                                       >  1:1103245/1‑150 (MQ=255)
                                                aGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGGGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGaa                                                                                                       >  1:2229574/1‑150 (MQ=255)
                                                       cAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAgcg                                                                                                <  1:822334/150‑1 (MQ=255)
                                                       cAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAgcg                                                                                                <  2:1040255/150‑1 (MQ=255)
                                                       cAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAgcg                                                                                                <  2:1972179/150‑1 (MQ=255)
                                                        aaTCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAgcgc                                                                                               >  1:2101580/1‑150 (MQ=255)
                                                          tcgcctcgccGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCa                                                                                             >  2:391163/1‑150 (MQ=255)
                                                             cctcgccGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGaa                                                                                          <  1:99243/150‑1 (MQ=255)
                                                              ctcgccGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAg                                                                                         >  1:1299817/1‑150 (MQ=255)
                                                               tcgccGAAACGCTTATAGTAACGTTTGATTAACGCCAGAATACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGTCGGTAAATCGGCGAAGAGAGCGCCAGAAGt                                                                                        >  2:2207114/1‑150 (MQ=255)
                                                                cgccGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGtt                                                                                       <  1:289875/150‑1 (MQ=255)
                                                                 gccGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTg                                                                                      <  2:836772/150‑1 (MQ=255)
                                                                 gccGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTg                                                                                      >  1:1538669/1‑150 (MQ=255)
                                                                      aaCGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCaa                                                                                 <  1:1781705/150‑1 (MQ=255)
                                                                        cGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGc                                                                               <  1:1442027/150‑1 (MQ=255)
                                                                          cTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTg                                                                             >  1:616530/1‑150 (MQ=255)
                                                                           ttATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGa                                                                            <  1:1005744/150‑1 (MQ=255)
                                                                            tataGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGaa                                                                           <  1:1363962/150‑1 (MQ=255)
                                                                            tataGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGaa                                                                           <  1:649220/150‑1 (MQ=255)
                                                                                 tAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACAttt                                                                      <  2:1986612/150‑1 (MQ=255)
                                                                                     gTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTttctt                                                                  >  2:216599/1‑150 (MQ=255)
                                                                                      tttGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTttcttc                                                                 >  2:1491497/1‑150 (MQ=255)
                                                                                      tttGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTttcttc                                                                 >  2:345735/1‑150 (MQ=255)
                                                                                      tttGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTttcttc                                                                 >  2:709885/1‑150 (MQ=255)
                                                                                       ttGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTtcttct                                                                >  2:1878723/1‑150 (MQ=255)
                                                                                              aCGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGt                                                         <  1:1488708/150‑1 (MQ=255)
                                                                                               cGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTg                                                        >  2:1521205/1‑150 (MQ=255)
                                                                                                gCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGc                                                       >  2:133446/1‑150 (MQ=255)
                                                                                                gCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGc                                                       >  1:528015/1‑150 (MQ=255)
                                                                                                gCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCACCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGc                                                       >  1:1264697/1‑150 (MQ=255)
                                                                                                   aGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAACTCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGc                                                    <  1:1605600/150‑1 (MQ=255)
                                                                                                   aGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGc                                                    <  1:638321/150‑1 (MQ=255)
                                                                                                     aTTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGGGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGccc                                                  <  1:1328608/150‑1 (MQ=255)
                                                                                                     aTTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGGGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGccc                                                  <  1:1328564/150‑1 (MQ=255)
                                                                                                     aTTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGGGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGccc                                                  <  2:1103245/150‑1 (MQ=255)
                                                                                                        aCGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTa                                               <  2:2049282/150‑1 (MQ=255)
                                                                                                        aCGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTa                                               >  1:1981053/1‑150 (MQ=255)
                                                                                                         cGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTaa                                              <  1:1252076/150‑1 (MQ=255)
                                                                                                          gCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAg                                             <  1:1962498/150‑1 (MQ=255)
                                                                                                          gCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAg                                             <  1:56405/150‑1 (MQ=255)
                                                                                                              tGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCaaa                                         >  1:1593697/1‑150 (MQ=255)
                                                                                                               gacgacATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCaaaa                                        <  2:1362750/150‑1 (MQ=255)
                                                                                                                acgacATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAg                                       <  2:1183099/150‑1 (MQ=255)
                                                                                                                      tAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCaa                                 >  2:1311550/1‑150 (MQ=255)
                                                                                                                      tAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCaa                                 >  2:397518/1‑150 (MQ=255)
                                                                                                                             gTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGgcg                          <  1:563834/150‑1 (MQ=255)
                                                                                                                             gTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGgcg                          <  1:13247/150‑1 (MQ=255)
                                                                                                                             gTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGgcg                          <  1:2343295/150‑1 (MQ=255)
                                                                                                                              tCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGgcgc                         >  1:814706/1‑150 (MQ=255)
                                                                                                                              tCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGgcgc                         >  2:748266/1‑150 (MQ=255)
                                                                                                                               cGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCa                        <  1:327053/150‑1 (MQ=255)
                                                                                                                                   aTAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGc                    >  2:289558/1‑150 (MQ=255)
                                                                                                                                   aTAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGc                    <  2:585158/150‑1 (MQ=255)
                                                                                                                                   aTAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGc                    >  1:152279/1‑150 (MQ=255)
                                                                                                                                   aTAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGc                    >  2:959494/1‑150 (MQ=255)
                                                                                                                                     aaaGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCt                  >  2:1826687/1‑150 (MQ=255)
                                                                                                                                     aaaGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCt                  >  2:174226/1‑150 (MQ=255)
                                                                                                                                      aaGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCTg                 >  2:1785529/1‑150 (MQ=255)
                                                                                                                                      aaGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCTg                 <  2:461260/150‑1 (MQ=255)
                                                                                                                                      aaGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCTg                 <  2:1206151/150‑1 (MQ=255)
                                                                                                                                        gCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCTGaa               <  2:120736/150‑1 (MQ=255)
                                                                                                                                          tGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCTGAATa             <  2:1387641/150‑1 (MQ=255)
                                                                                                                                             ggATCCTGAGCAAATAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCGAAAGTAGCAAAGTGGCGCAGTGCCTGAATAAAc          >  2:2290458/1‑150 (MQ=255)
                                                                                                                                             ggATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCTGAATAAAc          >  2:577216/1‑150 (MQ=255)
                                                                                                                                                tCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCTGAATAAACGcc       >  1:343424/1‑150 (MQ=255)
                                                                                                                                                     aGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCTGAATAAACGCCATAtt  >  1:1133746/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGTGCCTGAATAAACGCCATATT  >  NC_000913/2739030‑2739328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: