Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 71,999 T→G 12.0% L217V (TTG→GTG)  yabI → DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091371,9990TG12.0% 1623.0 / 79.7 627L217V (TTG→GTG) yabIDedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (198/340);  new base G (87/2);  total (285/342)
Fisher's exact test for biased strand distribution p-value = 5.56e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 668 total aligned reads displayed.

ATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACG  >  NC_000913/71850‑72148
                                                                                                                                                     |                                                                                                                                                       
 tagtCCGGCCGGAATGCAGCGCGGTGAGTTTACATGGGTGCGGCTGGCAACAGCGGTGTTTTTGTGGGTTGGGGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTACAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGttt                                                                                                                                                        <  2:6609208/147‑1 (MQ=255)
     ccTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTttgttg                                                                                                                                                    >  2:8097633/1‑150 (MQ=255)
       tGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTtttg                                                                                                                                                  >  1:9557101/1‑150 (MQ=255)
       tGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTtgtg                                                                                                                                                  >  1:8268543/1‑150 (MQ=255)
        gCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTgg                                                                                                                                                 <  1:2458269/150‑1 (MQ=255)
        gCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGGGTGAGTCATTATTTGTCCCGCGGTCGtgtggtgtgg                                                                                                                                                 >  1:3630345/1‑150 (MQ=255)
          cGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCt                                                                                                                                               >  2:6126482/1‑150 (MQ=255)
          cGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCt                                                                                                                                               <  2:2488363/150‑1 (MQ=255)
           ggAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTg                                                                                                                                              >  2:4829836/1‑150 (MQ=255)
             aaTGCAGAGCGGAGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGAc                                                                                                                                            <  2:3438880/150‑1 (MQ=255)
                   gagCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGtt                                                                                                                                      >  2:9072900/1‑150 (MQ=255)
                      cGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGAt                                                                                                                                   <  2:2198922/150‑1 (MQ=255)
                      cGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGAt                                                                                                                                   >  2:1751461/1‑150 (MQ=255)
                        gTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGGTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGTTGAttt                                                                                                                                 >  1:5714644/1‑150 (MQ=255)
                             tttAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGTCGCCGTTGATTTCTGcc                                                                                                                            >  2:8837916/1‑150 (MQ=255)
                                aaaTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGTCGCCGTTGATTTCTGcccta                                                                                                                         >  1:7968403/1‑147 (MQ=255)
                                 aaTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCg                                                                                                                        >  1:5434623/1‑150 (MQ=255)
                                  aTGGTTGCTGGTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGTCCGTTTGAGTCATTATTTGTCCCGCGGTGGTTTGTTGTGGGTGTCGGCGTTGTTTTGTGCCCTCgg                                                                                                                       >  1:3872439/1‑150 (MQ=255)
                                  aTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGGCGTGTGTTGTGGCTGACGCCGGTGCTTTCAGCCCTCgg                                                                                                                       >  2:8737010/1‑150 (MQ=255)
                                     gTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgt                                                                                                                    >  1:1421906/1‑150 (MQ=255)
                                     gTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgt                                                                                                                    <  1:7855920/150‑1 (MQ=255)
                                          tgctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGTGTCATTATGTGTCCCGCGGTCGGTGGTTTTGGCGGACGCCGGTGATTTATGCCCTCGGCGtggtgg                                                                                                               >  1:2778446/1‑150 (MQ=255)
                                          tgctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGtggtgg                                                                                                               <  2:5998847/150‑1 (MQ=255)
                                          tgctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGtggtgg                                                                                                               <  1:9408669/150‑1 (MQ=255)
                                          tgctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGtggtgg                                                                                                               >  2:4511796/1‑150 (MQ=255)
                                            ctgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCCTTATTTGTCCCGCGGTCGGGTGTTGTGGCTGACGCCGTTGAGTTCTGCCATCGGCGTGGTGgcg                                                                                                             >  2:9238208/1‑150 (MQ=255)
                                             tgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGGTGATTTCTGCCCACGGCGTGGTGgcgc                                                                                                            >  1:5053781/1‑150 (MQ=255)
                                             tgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATGATTTGTCCCGCGGGCGGGTGTTGTGGCTGACGCCGGTGAGTTATTCCATCGGCGGGGTGgcgc                                                                                                            >  1:4565649/1‑150 (MQ=255)
                                                cAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtgg                                                                                                         <  1:3438280/150‑1 (MQ=255)
                                                 aaCAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggt                                                                                                        <  1:4339482/150‑1 (MQ=255)
                                                 aaCAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggt                                                                                                        <  2:5181875/150‑1 (MQ=255)
                                                 aaCAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggt                                                                                                        <  2:5062516/150‑1 (MQ=255)
                                                 aaCAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggt                                                                                                        <  1:5595118/150‑1 (MQ=255)
                                                    aGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTggtggt                                                                                                     <  1:5385081/150‑1 (MQ=255)
                                                    aGCGGTGTTTTTGTGGGTTGGTGGCTGGCCGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTggtggt                                                                                                     <  1:6727147/150‑1 (MQ=255)
                                                     gCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGgtggtg                                                                                                    <  1:9905955/150‑1 (MQ=255)
                                                      cGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGgtgt                                                                                                   <  1:1982038/150‑1 (MQ=255)
                                                           tttgtGGGGGGTGGGGGCTGGCTGTGCTGGCGGTTCTGGCGCAGCGGTAACGCGACTGACCGTTTGAGTCATGCTTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAAtt                                                                                              <  1:500088/150‑1 (MQ=255)
                                                             tttGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCg                                                                                            <  1:9172783/150‑1 (MQ=255)
                                                                 tgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGGCGTTGATTTATTCCATCGGCGTGGTGGCGCTGGTGGGGTTTATTCGCCAc                                                                                        >  1:8817235/1‑150 (MQ=255)
                                                                   ggTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAccc                                                                                      <  1:6884744/150‑1 (MQ=255)
                                                                   ggTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAccc                                                                                      <  1:3291061/150‑1 (MQ=255)
                                                                   ggTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTTGCTGACGCCGGTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAccc                                                                                      >  2:2781781/1‑150 (MQ=255)
                                                                   ggTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGGTGATTTCTGCCATCGGCGTGGTGGCGCTGGGGGTGTTAATTCGCCAccc                                                                                      >  2:9660074/1‑150 (MQ=255)
                                                                         tggctggcTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTTGCTGAAGCCGGTGATTTATTCCAACCGCCTGGTGGCGCAGGTGGTGTTAATTCGCCACCCCTTGAt                                                                               >  1:8071999/1‑150 (MQ=255)
                                                                         tggctggcTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGAt                                                                               <  1:2619956/150‑1 (MQ=255)
                                                                          ggctggctGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTCTTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGGTGATTTCTGCCATCCGCGTGGTGGCGCTGGTGGGGTTAATTAGcccccccgggctg                                                                               >  1:1208426/1‑143 (MQ=255)
                                                                            ctggctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGTCTGACCGTTTGGGTCATTAGTTGTCCCGCGGTCGTGTGGTGTGGCTGACGCCGTTGATTTCTGCCCTCGGCGTGGTGGCGCTGGTGGTGTTACTTCGCCACCCGTTGATGcc                                                                            >  2:2568724/1‑150 (MQ=255)
                                                                               gctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgt                                                                        >  2:1006077/1‑150 (MQ=255)
                                                                                ctgTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgtg                                                                       <  2:2889663/150‑1 (MQ=255)
                                                                                 tgtgCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgtgt                                                                      >  2:5947458/1‑150 (MQ=255)
                                                                                 tgtgCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGTCGCCGGTGTTTTCTGCCCACGGCGTGGTGGCGCTGGTGGTGTTTATTCGCCCCCCGGTGATGCCGgggt                                                                      >  1:7452281/1‑150 (MQ=255)
                                                                                  gtgCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGta                                                                     <  1:648467/150‑1 (MQ=255)
                                                                                   tgCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGACGCCGGTGATTTCTGCCATCGGCGGGGTGGCGCGGGTGGTGTTAATTCGCCACCCCTTGTTGCCGGTGtat                                                                    >  2:7788215/1‑150 (MQ=255)
                                                                                     cTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTatat                                                                  <  1:4752372/150‑1 (MQ=255)
                                                                                     cTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTatat                                                                  <  1:1751461/150‑1 (MQ=255)
                                                                                     cTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTatat                                                                  >  2:5648746/1‑150 (MQ=255)
                                                                                      tGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTTTGGCGGACGCCGGTGGTTTATGCCCTCGGCGTGGGGGCGCTGGTGGGGGTAATTAGCCACCCCGTGTTGCCGGGGTatatg                                                                 >  1:4832640/1‑149 (MQ=255)
                                                                                      tGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGGTTGTTGTTGCTGAAGCCCGTGATTTATTCCAACGGCCTGGTGGGGCTTGTGGGGTTAATACACCACCCGATTGTGGCCGTGTGtata                                                                   >  1:6266086/1‑150 (MQ=255)
                                                                                             tATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGGCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATAttt                                                          <  2:2133113/150‑1 (MQ=255)
                                                                                               tGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTg                                                        <  1:8506379/150‑1 (MQ=255)
                                                                                               tGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGGTGATTTGTGCCAAAGGCGTGGTGGCGCGGGTGGTGGTAATTAGCCACCCCGTGATGCCGGGGTATATCGATAttttt                                                        >  1:7499964/1‑149 (MQ=255)
                                                                                                ggCGCAGCGGTAAAGCGACTGACCGTTTGAGTGATTATTTGTCCCGCGGTCGTTTTTTGTTGCTGACGCCGTTGTTTTGTTGCATAGGGGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATAtttttg                                                       >  2:309803/1‑148 (MQ=255)
                                                                                                ggCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGc                                                       <  2:1884491/150‑1 (MQ=255)
                                                                                                ggCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGc                                                       <  1:7073060/150‑1 (MQ=255)
                                                                                                 gcgcAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTAGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCg                                                      >  2:2710443/1‑150 (MQ=255)
                                                                                                    cAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTaa                                                   <  1:157557/150‑1 (MQ=255)
                                                                                                        ggTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTg                                               <  2:4612267/150‑1 (MQ=255)
                                                                                                        ggTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTg                                               <  1:2225247/150‑1 (MQ=255)
                                                                                                           aaaGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATGTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGtt                                            <  2:5736482/150‑1 (MQ=255)
                                                                                                            aaGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTg                                           <  2:947048/150‑1 (MQ=255)
                                                                                                               cGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTgggg                                        <  2:1433291/150‑1 (MQ=255)
                                                                                                                  cTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGttt                                     <  2:7933856/150‑1 (MQ=255)
                                                                                                                   tGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTa                                    <  2:6457455/150‑1 (MQ=255)
                                                                                                                   tGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTa                                    <  2:7599224/150‑1 (MQ=255)
                                                                                                                   tGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTa                                    <  2:8449310/150‑1 (MQ=255)
                                                                                                                   tGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTGTGTTGTGGCTGAAGCCGTTGATTTCTGCCAACCGCCTGGTGGCGCTGGTGGTGTTAATTCGCCCCCCCGTGATGCCGGTGTATATCGAGAATTTTCGGAAAGTGGTTGGGGTTTa                                    >  2:3937057/1‑150 (MQ=255)
                                                                                                                     aCCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTATGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTAGCCACCCCTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAgg                                  >  2:239672/1‑150 (MQ=255)
                                                                                                                      ccGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGga                                 <  2:5477526/150‑1 (MQ=255)
                                                                                                                        gTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTTCGTAAAGTGGTTGGGGTTTAGgaga                               >  2:4330866/1‑150 (MQ=255)
                                                                                                                        gTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGgaga                               <  1:10237105/150‑1 (MQ=255)
                                                                                                                          ttGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATa                             <  2:1111977/150‑1 (MQ=255)
                                                                                                                             aGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTc                          <  2:7220593/150‑1 (MQ=255)
                                                                                                                              gTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATAGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCt                         >  2:5274795/1‑150 (MQ=255)
                                                                                                                               tCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTTCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTAtt                        >  2:7477327/1‑150 (MQ=255)
                                                                                                                                 attattTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTtgt                      <  2:421582/150‑1 (MQ=255)
                                                                                                                                     tttGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCgg                  <  1:1349458/150‑1 (MQ=255)
                                                                                                                                     tttGTCCCGCGGTCGTTTGTTGTGGCATACGCCGTTGAATTATGCCAAAGGCGTGGTTGCGCAGGTGGTGTTAATTCGCCACCCGAAGATTCCGGTGTAAATAGATAATTTTCGTAAATTGGTTGGGGTTTACTAGATAGTCtttttctg                  >  2:6204269/1‑144 (MQ=255)
                                                                                                                                         tCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTg              <  1:299602/150‑1 (MQ=255)
                                                                                                                                           ccGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGc             <  1:6010697/149‑1 (MQ=255)
                                                                                                                                            cgcgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTAGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTTGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCt           >  2:7155812/1‑150 (MQ=255)
                                                                                                                                            cgcgGTCGTGTGTTGTGGCTGACGCCGGTGATTTCAGCCATCGGCCTGGTGGCGCTTGTGGTGTTAAATCGCCACCCCGTGATTCCCGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTATGAGATAGTCATGTGCGGGTTTCCt           >  2:1992126/1‑150 (MQ=255)
                                                                                                                                              cgGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGa         <  2:5103303/150‑1 (MQ=255)
                                                                                                                                                gTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATg       <  1:1006077/150‑1 (MQ=255)
                                                                                                                                                 tCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGc      >  2:8570318/1‑150 (MQ=255)
                                                                                                                                                   gTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGa    >  2:1553840/1‑150 (MQ=255)
                                                                                                                                                   gTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGa    >  2:6893802/1‑150 (MQ=255)
                                                                                                                                                    tttGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTTTGCGGGTTGCCTGATGCGAc   >  1:8943345/1‑150 (MQ=255)
                                                                                                                                                    tttGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGAc   >  1:6804473/1‑150 (MQ=255)
                                                                                                                                                     ttgttgTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTCATGCGACg  >  2:8287150/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
ATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACG  >  NC_000913/71850‑72148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: