Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,630 G→C 6.3% pseudogene (1239/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,6300GC 6.3% 1187.3 / 47.3 369pseudogene (1239/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base G (192/144);  new base C (11/13);  total (210/159)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 374 total aligned reads displayed.

TTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGA  >  NC_000913/1466482‑1466779
                                                                                                                                                    |                                                                                                                                                     
  gATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAg                                                                                                                                                    >  1:5905516/1‑150 (MQ=255)
       cACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTaa                                                                                                                                               >  1:8144881/1‑150 (MQ=255)
        aCCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAAc                                                                                                                                              <  1:1333562/150‑1 (MQ=255)
        aCCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAAc                                                                                                                                              >  1:2970161/1‑150 (MQ=255)
        aCCGGCGAAAGCGCAACGGTGCATAACAAGGGCACCAAGACAGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACAAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAAc                                                                                                                                              <  2:580347/150‑1 (MQ=18)
         ccGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACg                                                                                                                                             >  1:1090004/1‑150 (MQ=255)
          cGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACgg                                                                                                                                            >  1:8751327/1‑150 (MQ=255)
           ggCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGt                                                                                                                                           <  1:7678282/150‑1 (MQ=255)
             cGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtga                                                                                                                                         <  2:3048051/150‑1 (MQ=255)
              gACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCAGCGGTCTCCAGGTTGACGGCGCCCAGGCGGGTGGTCACAACGAAGGCGAGAGCGCCATCACCACCGGTGGCCCCGGCACGCCGAGTAACGGggat                                                                                                                                        >  1:4866425/1‑150 (MQ=18)
                 aGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACTCAGATCAACGGTAACGAc                                                                                                                                     >  1:2001742/1‑150 (MQ=255)
                 aGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGAc                                                                                                                                     <  1:9420948/150‑1 (MQ=255)
                   cgcAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTGGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGc                                                                                                                                   <  1:7817739/150‑1 (MQ=255)
                             gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACTCAGATCAACGGTGAAGACGCCACCGCgaaa                                                                                                                         >  1:4117231/1‑149 (MQ=14)
                             gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaac                                                                                                                         >  2:9646970/1‑150 (MQ=255)
                                aaCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCCGTTGTAAACAACGAAGGCGAGAGCGCAATCACCAACGGGGGCACCGGCACGCAGATAAACGGTCACGACGCCACGGCCaaaaac                                                                                                                      >  2:6242157/1‑150 (MQ=14)
                                      ggCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGc                                                                                                                >  1:10024533/1‑150 (MQ=255)
                                        cACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCaa                                                                                                              >  1:6243911/1‑150 (MQ=255)
                                         aCCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGTTTGACGGCGACCAGGCGGTTGTTAAGAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCaaa                                                                                                             <  2:6588291/150‑1 (MQ=255)
                                              gACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccac                                                                                                        >  2:2782233/1‑150 (MQ=255)
                                              gACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccac                                                                                                        >  1:6051322/1‑150 (MQ=255)
                                              gACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccac                                                                                                        <  1:494918/150‑1 (MQ=255)
                                              gACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCAATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccac                                                                                                        <  1:4655310/150‑1 (MQ=255)
                                               aCCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGTTGACGCCACGGCAAACAACAACGGCAAAaccacc                                                                                                       <  1:219336/150‑1 (MQ=255)
                                                 cGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTAAAAAACGAAGGCGAGAGCGCCATCACCAAAGGAGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGAAAAACCACAGt                                                                                                     >  2:9193870/1‑150 (MQ=17)
                                                   tCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAAAAACAGAGGAAAAACCACTGTTg                                                                                                   >  2:3491008/1‑150 (MQ=11)
                                                   tCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATAAACGGTTAAGACGCCACCGCAAAAAACAAAGGCAAAACCACAGTTg                                                                                                   >  2:5146823/1‑150 (MQ=18)
                                                     aCCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGAc                                                                                                 >  1:1129056/1‑150 (MQ=255)
                                                       cGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg                                                                                               >  1:5069395/1‑150 (MQ=255)
                                                        gATCCGGAGTCCATCGGTATCCAGGTTGACGGAGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCAACAACGGTGGCACCGGCACTCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGc                                                                                              >  2:8328978/1‑150 (MQ=255)
                                                         aTCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCa                                                                                             >  2:9507114/1‑150 (MQ=255)
                                                         aTCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAAAGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACTGCAAAACCACCGTTGACGGCa                                                                                             >  1:2523364/1‑150 (MQ=255)
                                                         aTCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCCGTAGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGAAGACGCCACAGCCAAAAACAAAGGCAAAACCACCGTTGAAggaa                                                                                             >  2:9355798/1‑148 (MQ=17)
                                                          tCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa                                                                                            <  2:10109166/150‑1 (MQ=255)
                                                          tCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa                                                                                            <  2:826640/150‑1 (MQ=255)
                                                            cGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAgg                                                                                          >  1:4443020/1‑150 (MQ=255)
                                                                 tCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTcc                                                                                     >  1:9324647/1‑150 (MQ=255)
                                                                  ccATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCa                                                                                    <  1:98898/150‑1 (MQ=255)
                                                                   cagcGGGATCCAGGAGGCCGGAGACCAGGCGGTTGCTAACAACGACGGCGAGAGCCCCATCACCTACGTTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCTAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc                                                                                   <  2:8109520/147‑1 (MQ=17)
                                                                   cATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc                                                                                   <  1:7289159/150‑1 (MQ=255)
                                                                    aTCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATAAACGGTGATGACGCCCCGGCAAACAACAACTGCAAATCCAACTGTGACGGCGAGGATTCCAcc                                                                                  >  2:8684183/1‑150 (MQ=18)
                                                                     tCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCg                                                                                 <  2:4645055/150‑1 (MQ=255)
                                                                     tCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCg                                                                                 <  1:5332394/150‑1 (MQ=255)
                                                                        gTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTa                                                                              >  2:7060190/1‑150 (MQ=255)
                                                                        gTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTa                                                                              >  1:3744179/1‑150 (MQ=255)
                                                                        gTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAAAGGAGGCACCGGCACTCAGATAAACGGTCAAGACGCCACCGCGAAAAACAAAGGAAAAACCACAGTTGAAGGAAAGGATTCCACCGGTa                                                                              >  2:3811705/1‑150 (MQ=255)
                                                                           tCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACgg                                                                           >  1:2834196/1‑150 (MQ=255)
                                                                           tCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATGAACGGTGAGGACGCCACCGCGAAAAACAAAGGAAAAACCACCGTTGAAGGAAATGATTCCACCGGTAccc                                                                           >  1:8864646/1‑148 (MQ=18)
                                                                              aGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaaa                                                                        >  1:5514262/1‑150 (MQ=255)
                                                                               ggTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAt                                                                       >  1:5705603/1‑150 (MQ=255)
                                                                                gTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAtt                                                                      >  1:2692924/1‑150 (MQ=255)
                                                                                     cGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTgg                                                                 <  2:1246155/150‑1 (MQ=255)
                                                                                      ggCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCACCGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGc                                                                <  1:6584794/150‑1 (MQ=255)
                                                                                       gCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCa                                                               >  1:9208504/1‑150 (MQ=255)
                                                                                        cGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCaa                                                              >  2:5617554/1‑150 (MQ=255)
                                                                                         gACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAAt                                                             >  1:3281061/1‑150 (MQ=255)
                                                                                         gACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAAt                                                             >  1:6509650/1‑150 (MQ=255)
                                                                                            cAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAAc                                                          >  1:6938192/1‑150 (MQ=255)
                                                                                             aGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACg                                                         <  1:5807063/150‑1 (MQ=255)
                                                                                              ggCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACgg                                                        <  1:1473043/150‑1 (MQ=255)
                                                                                                 ggTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGAGGCACCGGCACGCAGATAAACGGTGAAGACGCCACGGCCAACAACAACGGCAAAACCACCGTAGACGGCAAGGATTCCACCGGGACGGAACTAGCCGGCAATACCGGGaa                                                     >  1:807927/1‑150 (MQ=17)
                                                                                                  gttgttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAg                                                    <  2:1129056/150‑1 (MQ=255)
                                                                                                  gttgttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAg                                                    >  1:1598288/1‑150 (MQ=255)
                                                                                                    tgttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCTGCACGCATATTAACGGTGATGACGCCAATGAAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGt                                                  <  2:6905223/150‑1 (MQ=18)
                                                                                                      ttAATAACGAAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGa                                                <  2:6075729/150‑1 (MQ=255)
                                                                                                      ttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGa                                                <  1:4594608/150‑1 (MQ=255)
                                                                                                      ttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGa                                                >  2:8563863/1‑150 (MQ=255)
                                                                                                      ttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGa                                                <  2:5346085/150‑1 (MQ=255)
                                                                                                      tgaacaacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGa                                                <  1:9125450/148‑1 (MQ=255)
                                                                                                         acaacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCACTAACGGGACGGTGAGTc                                             >  1:10106677/1‑150 (MQ=255)
                                                                                                           aacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAg                                           >  2:2357889/1‑150 (MQ=255)
                                                                                                           aacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAg                                           <  2:5069395/150‑1 (MQ=255)
                                                                                                           aacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAg                                           <  2:289833/150‑1 (MQ=255)
                                                                                                             cGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGa                                         >  1:54581/1‑150 (MQ=255)
                                                                                                             cGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGa                                         <  2:8415950/150‑1 (MQ=255)
                                                                                                              gAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGAc                                        >  2:4320043/1‑150 (MQ=255)
                                                                                                               aaGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaa                                                                         <  2:5496433/116‑1 (MQ=39)
                                                                                                               aaGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACg                                       >  1:3434298/1‑150 (MQ=255)
                                                                                                               aaGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGAtt                                              <  1:8308885/143‑1 (MQ=255)
                                                                                                                    gagaGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTa                                                                              <  1:9453731/106‑1 (MQ=17)
                                                                                                                        gcgcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGACAGTGCTGGCACTCACGGGAAGGTGATTCAGGACGGCGATCTgg                              >  1:8213039/1‑150 (MQ=255)
                                                                                                                         cgcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGa                             <  2:1428266/150‑1 (MQ=255)
                                                                                                                         cgcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGa                             >  2:1651639/1‑150 (MQ=255)
                                                                                                                           ccATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATg                           >  2:1984699/1‑150 (MQ=255)
                                                                                                                            cATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAGTGGAAAAacca                                                                                                         >  1:4006888/1‑71 (MQ=14)
                                                                                                                             aTCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGAc                                                                                                 <  1:1769039/78‑1 (MQ=39)
                                                                                                                             aTCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTc                         >  2:4547183/1‑150 (MQ=255)
                                                                                                                              tCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCa                        >  2:8867980/1‑150 (MQ=255)
                                                                                                                                aCCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATAGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgc                      >  2:9311918/1‑150 (MQ=255)
                                                                                                                                 ccAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcg                     >  1:9564164/1‑150 (MQ=255)
                                                                                                                                  cAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCa                                                                                    <  1:9776990/86‑1 (MQ=38)
                                                                                                                                     cGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcgg                 <  2:2948582/150‑1 (MQ=255)
                                                                                                                                      ggTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcggc                <  2:2096022/150‑1 (MQ=255)
                                                                                                                                       gTGGCACCGTCACCCAGATTAAGGGTTATGACGCCACGGCAAACAACAACGTCAAACACACCGTTGACGGCAAGGATTCCACCGTTACGTAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcggcg               <  2:7781640/150‑1 (MQ=14)
                                                                                                                                           cACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa           <  1:8563863/150‑1 (MQ=255)
                                                                                                                                           cACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa           >  2:4013775/1‑150 (MQ=255)
                                                                                                                                           cACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCa           <  1:2098406/150‑1 (MQ=255)
                                                                                                                                              cGGCACCCAGATTAATGGTGATGACGCCACGTCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACgg        <  2:4242075/150‑1 (MQ=255)
                                                                                                                                                    gCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGACATTGCTGGCAAGAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa  >  2:7252070/1‑150 (MQ=255)
                                                                                                                                                    gCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa  >  1:9666006/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGA  >  NC_000913/1466482‑1466779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: