Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,630,528 T→G 6.0% Y129S (TAT→TCT)  ydfI ← putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,630,5280TG 6.0% 2102.5 / 89.7 613Y129S (TAT→TCT) ydfIputative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (286/289);  new base G (18/19);  total (305/308)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 618 total aligned reads displayed.

CGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGACATTTCC  >  NC_000913/1630379‑1630677
                                                                                                                                                     |                                                                                                                                                     
cGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAt                                                                                                                                                       <  2:6332253/150‑1 (MQ=255)
  gTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGACATAc                                                                                                                                                     <  2:9856233/150‑1 (MQ=255)
  gTAAATGCGGGAAGTCCTGCCGCTGTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATAc                                                                                                                                                     <  2:7323461/150‑1 (MQ=255)
           ggAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCg                                                                                                                                            <  2:7547585/150‑1 (MQ=255)
           ggAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCg                                                                                                                                            <  2:242867/150‑1 (MQ=255)
            gAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCgg                                                                                                                                           <  1:4425826/150‑1 (MQ=255)
             aaGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGt                                                                                                                                          <  1:8612070/150‑1 (MQ=255)
               gTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGa                                                                                                                                        >  2:7096469/1‑150 (MQ=255)
                tCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCAATAACTGTCCGGTCGCCGGAGAGTGGAAATACCCATTTTCGGTGAt                                                                                                                                       >  1:5021691/1‑150 (MQ=255)
                    gCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGAGTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTc                                                                                                                                   >  1:7490484/1‑150 (MQ=255)
                     ccGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCa                                                                                                                                  <  1:7670954/150‑1 (MQ=255)
                     ccGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCa                                                                                                                                  >  1:8308325/1‑150 (MQ=255)
                       gCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCaga                                                                                                                                <  1:10069703/150‑1 (MQ=255)
                        cTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCagag                                                                                                                               <  2:4190863/150‑1 (MQ=255)
                         tttACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCagaga                                                                                                                              <  2:6872071/150‑1 (MQ=255)
                         tgtACGGCGAGCCAGCGCCTCAACAATCACCCCGGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCagaga                                                                                                                              <  1:3747509/148‑1 (MQ=255)
                            aCGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGAc                                                                                                                           <  1:4279088/150‑1 (MQ=255)
                            aCGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGAc                                                                                                                           >  1:2575717/1‑150 (MQ=255)
                              ggCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACaa                                                                                                                         >  2:31287/1‑150 (MQ=255)
                              ggCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACaa                                                                                                                         <  1:1122487/150‑1 (MQ=255)
                              ggCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACaa                                                                                                                         >  2:6058907/1‑150 (MQ=255)
                              ggCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACaa                                                                                                                         <  2:6149125/150‑1 (MQ=255)
                              ggCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACaa                                                                                                                         >  1:5820233/1‑150 (MQ=255)
                                cGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATc                                                                                                                       <  1:7203118/150‑1 (MQ=255)
                                 gAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcg                                                                                                                      >  2:10268582/1‑150 (MQ=255)
                                 gAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATcg                                                                                                                      >  2:8158699/1‑150 (MQ=255)
                                    ccAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGa                                                                                                                   >  1:8145173/1‑150 (MQ=255)
                                     cAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAt                                                                                                                  >  1:3170780/1‑150 (MQ=255)
                                     cAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGAt                                                                                                                  >  1:7427003/1‑150 (MQ=255)
                                        cgcCTCAACACTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTg                                                                                                               <  2:2598533/150‑1 (MQ=255)
                                        cgcCTCAACACTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTg                                                                                                               <  1:5216111/150‑1 (MQ=255)
                                          ccTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCg                                                                                                             <  2:8626522/150‑1 (MQ=255)
                                           cTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCgg                                                                                                            <  1:2988373/150‑1 (MQ=255)
                                           cTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCgg                                                                                                            >  2:380903/1‑150 (MQ=255)
                                           cTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCgg                                                                                                            >  1:9575637/1‑150 (MQ=255)
                                           cTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATAGCGATTTGCgg                                                                                                            >  2:1254981/1‑150 (MQ=255)
                                             caacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGtt                                                                                                          <  1:4688635/150‑1 (MQ=255)
                                             caacaaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGtt                                                                                                          <  1:9820231/150‑1 (MQ=255)
                                                caaTCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTcac                                                                                                       <  1:1647534/150‑1 (MQ=255)
                                                   tCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTcacaca                                                                                                    >  1:796892/1‑150 (MQ=255)
                                                       cccTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGc                                                                                                <  2:3247737/150‑1 (MQ=255)
                                                        ccTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCt                                                                                               <  2:5167486/150‑1 (MQ=255)
                                                        ccTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCt                                                                                               <  2:3694453/150‑1 (MQ=255)
                                                               cTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAaca                                                                                        >  1:2322932/1‑150 (MQ=255)
                                                                 gTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTca                                                                                                        <  2:1516743/132‑1 (MQ=255)
                                                                 gTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACg                                                                                      <  2:3609446/150‑1 (MQ=255)
                                                                 gTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACg                                                                                      >  2:7932504/1‑150 (MQ=255)
                                                                   tttCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGt                                                                                    >  2:830263/1‑150 (MQ=255)
                                                                         cTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTaa                                                                              <  2:3187724/150‑1 (MQ=255)
                                                                         cTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTaa                                                                              <  1:1331348/150‑1 (MQ=255)
                                                                         cTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTaa                                                                              <  1:8807231/150‑1 (MQ=255)
                                                                             tggGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTTAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCa                                                                          <  2:4012936/150‑1 (MQ=255)
                                                                             tggGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCa                                                                          >  2:6461636/1‑150 (MQ=255)
                                                                             tggGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCa                                                                          <  1:5372284/150‑1 (MQ=255)
                                                                             tggGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCa                                                                          <  1:6595929/150‑1 (MQ=255)
                                                                              ggggATTTTGCTCGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCat                                                                         <  1:6068387/150‑1 (MQ=255)
                                                                                gggatttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatct                                                                       >  2:9613604/5‑150 (MQ=255)
                                                                                ggATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatct                                                                       <  2:481335/150‑1 (MQ=255)
                                                                                ggATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatct                                                                       <  1:3828571/150‑1 (MQ=255)
                                                                                ggATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatct                                                                       >  2:2977237/1‑150 (MQ=255)
                                                                                 gATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatcta                                                                      >  1:631829/1‑150 (MQ=255)
                                                                                   ttttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatctatc                                                                    >  1:4072125/1‑150 (MQ=255)
                                                                                   ttttGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCatctatc                                                                    <  1:6518539/150‑1 (MQ=255)
                                                                                      tGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGt                                                                 >  1:9349164/1‑150 (MQ=255)
                                                                                       gCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTa                                                                >  2:8988441/1‑150 (MQ=255)
                                                                                              ggCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCaa                                                         <  2:8222490/150‑1 (MQ=255)
                                                                                               gCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAg                                                        >  2:3091301/1‑150 (MQ=255)
                                                                                               gCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAg                                                        >  2:9350181/1‑150 (MQ=255)
                                                                                                 aGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGc                                                      >  2:557345/1‑150 (MQ=255)
                                                                                                   cTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCtt                                                    <  2:9223198/150‑1 (MQ=255)
                                                                                                    tACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCttt                                                   >  1:8988825/1‑150 (MQ=255)
                                                                                                     aCCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGGTTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCtttt                                                  <  1:4910926/150‑1 (MQ=255)
                                                                                                     aCCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCtttt                                                  <  2:7886729/150‑1 (MQ=255)
                                                                                                      ccATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCttttt                                                 <  2:8539659/150‑1 (MQ=255)
                                                                                                         tCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTaa                                              <  1:8499757/150‑1 (MQ=255)
                                                                                                         tCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTaa                                              <  1:1888450/150‑1 (MQ=255)
                                                                                                         tCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTaa                                              >  2:8438108/1‑150 (MQ=255)
                                                                                                         tCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTaa                                              <  1:7053636/150‑1 (MQ=255)
                                                                                                          cGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAac                                             <  1:2747553/150‑1 (MQ=255)
                                                                                                          cGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTCTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAac                                             <  1:6763438/150‑1 (MQ=255)
                                                                                                            ggTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAacga                                           <  2:1664079/150‑1 (MQ=255)
                                                                                                              tGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGc                                        <  2:2147374/150‑1 (MQ=255)
                                                                                                               gATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGc                                        <  2:499801/150‑1 (MQ=255)
                                                                                                                   gAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAac                                    >  2:744700/1‑150 (MQ=255)
                                                                                                                   gAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAac                                    >  2:4350032/1‑150 (MQ=255)
                                                                                                                   gAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAac                                    >  1:9154295/1‑150 (MQ=255)
                                                                                                                      cATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAacgac                                 >  2:2345532/1‑150 (MQ=255)
                                                                                                                       aTTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAacgacg                                >  1:5544018/1‑150 (MQ=255)
                                                                                                                         tAACTGTCCGGTCGCCGGAGAGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGAcgcg                              >  1:3402369/1‑150 (MQ=255)
                                                                                                                          aaCTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGa                             <  2:4570688/150‑1 (MQ=255)
                                                                                                                            cTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGc                           >  2:6614069/1‑150 (MQ=255)
                                                                                                                               tCCGGTAGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGt                        >  2:2869957/1‑150 (MQ=255)
                                                                                                                               gccGGTCGCCGGAGTGTGGAAAGACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGt                        <  1:4184337/149‑1 (MQ=255)
                                                                                                                                 cGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTcc                      >  2:9076305/1‑150 (MQ=255)
                                                                                                                                  ggTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGGTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCca                     >  1:1510949/1‑150 (MQ=255)
                                                                                                                                    tCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGTCGCGAGCCGTCcaca                   <  2:5302677/150‑1 (MQ=255)
                                                                                                                                         ggAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCg              <  2:1839800/150‑1 (MQ=255)
                                                                                                                                           agagTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGc            >  2:3923209/1‑150 (MQ=255)
                                                                                                                                              gTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGa         >  1:853541/1‑150 (MQ=255)
                                                                                                                                                ggAAAGACCCTCTCTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGACa       <  2:5844692/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGATTTTGCACGTCGGCAGCTACCATCGGGTGATCGAGCATTAACTGTCCGGTCGCCGGAGAGTGGAAATACCCTTTTTCGGTGATTGTCAGAGAGACAATCGCGATTTGCGGTTCACACATCGCTGCCAACACGGTTTCTAAGCCATCTATCTGTACGTGCAAGGCTTTTTTAACGACGCCAACGACGCGAGCCGTCCACACATCGGCCGACATTTCC  >  NC_000913/1630379‑1630677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: