Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,662,370 A→C 6.4% K605N (AAA→AAC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,662,3700AC 6.4% 1238.1 / 13.0 383K605N (AAA→AAC) ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (280/69);  new base C (2/32);  total (282/101)
Fisher's exact test for biased strand distribution p-value = 1.67e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 417 total aligned reads displayed.

TACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTG  >  NC_000913/1662221‑1662519
                                                                                                                                                     |                                                                                                                                                     
tACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGaaa                                                                                                                                                       >  2:6779811/1‑150 (MQ=255)
 aCTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGaaaa                                                                                                                                                      <  1:4106340/150‑1 (MQ=255)
  cTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAAc                                                                                                                                                     <  2:4786296/150‑1 (MQ=255)
  cTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAAc                                                                                                                                                     <  1:3333748/150‑1 (MQ=255)
     gATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGAc                                                                                                                                                  >  1:9625513/1‑150 (MQ=255)
            aGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATc                                                                                                                                           >  2:7364432/1‑150 (MQ=255)
            aGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATc                                                                                                                                           >  2:3020782/1‑150 (MQ=255)
            aGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATc                                                                                                                                           >  2:7135127/1‑150 (MQ=255)
            aGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATc                                                                                                                                           >  2:7858676/1‑150 (MQ=255)
            aGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATc                                                                                                                                           >  1:6820001/1‑150 (MQ=255)
                     gCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaa                                                                                                                                  >  1:825804/1‑150 (MQ=255)
                      ccAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaa                                                                                                                                 >  2:3684129/1‑150 (MQ=255)
                       aaaaCGATTCGTACCATACTTTTAAAAACCCTTTCCTGACGGCCCCAGTCCCCTTTATTGTAAAAAATATCACCTTGCTAAAACGACGCAACTTAACCCTGAGAGGCCCCACTACGTGGAGTTGAAAACGACCGGGATCTTTAAGaaaaa                                                                                                                                <  2:8639173/149‑1 (MQ=255)
                        aaaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaaaa                                                                                                                               >  1:10232280/1‑150 (MQ=255)
                        aaaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaaaa                                                                                                                               >  1:9273226/1‑150 (MQ=255)
                        aaaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaaaa                                                                                                                               >  1:7958497/1‑150 (MQ=255)
                        aaaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaaaa                                                                                                                               >  1:7038759/1‑150 (MQ=255)
                         aaCGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAAt                                                                                                                              >  1:779041/1‑150 (MQ=255)
                             gtttggACCTGCCTTTTATCAAACATTTAGTTACGGTGTCTGTAAGATTTATTGTATCATATATCTCCATGCTAAAAGGAATTAACTTAACCCTGAGTTGCCCGACTCCGAGGATTTGAAAACGACCGGGATCTTTAAGAAAAAATGccc                                                                                                                          <  2:8946696/146‑1 (MQ=255)
                              ttAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCg                                                                                                                         >  2:5456611/1‑150 (MQ=255)
                              ttAGGACCAGACGTTTATCAAAACTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCg                                                                                                                         >  1:1168338/1‑150 (MQ=255)
                                  gACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaag                                                                                                                     >  2:3730467/1‑150 (MQ=255)
                                  gACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaag                                                                                                                     >  2:5314076/1‑150 (MQ=255)
                                    ccAGTCTTTTATCAAACCTTTACTGATGGTCGAATTCAGAATTATTGGATCAATTATCTCAATGCTAAAACGATGTAAGGTAACCCTGTTATGCCCTACTAGGTGGTGTTTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGgaagaa                                                                                                                   <  2:8129069/150‑1 (MQ=255)
                                       gACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAacac                                                                                                                >  2:2643883/1‑150 (MQ=255)
                                          gTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTAc                                                                                                             >  2:5978792/1‑150 (MQ=255)
                                             taaaaacccTTTACTGTTGGCCGCAGACAGCATCATTGGCTAAATTATCGACCGGAGAAAACGCAGGAACGTAACCCTTAGCTGCCCGACTACGTGGCTACCAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTc                                                                                                          <  2:9530433/143‑1 (MQ=255)
                                             tATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTc                                                                                                          >  2:2170481/1‑150 (MQ=255)
                                              aTCAACGCTTTCGTGACGGTCGCAGTCAGCTTTACTGTTTAAAATATCCCCTTGCTAAACCGATGGACCTTAACCCTGAGAGGCCCCACTACTAGTTTTTCAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCg                                                                                                         <  2:9761100/150‑1 (MQ=255)
                                                 aaaCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCtt                                                                                                      >  2:1260517/1‑150 (MQ=255)
                                                   aCCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTc                                                                                                    >  1:160261/1‑150 (MQ=255)
                                                     cTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcg                                                                                                  >  2:3972545/1‑150 (MQ=255)
                                                     cTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcg                                                                                                  >  1:2412015/1‑150 (MQ=255)
                                                      tttACTGATGGTCGCAGTCTGCTTGATTGGATCAAATATCTCCATGCGAACACGAAGGAACGTAACCCTGAGATGCCCGCCTACGAGGATTTGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcgc                                                                                                 <  2:10028290/150‑1 (MQ=255)
                                                      tttACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCcgc                                                                                                 >  2:2015301/1‑150 (MQ=255)
                                                        tACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCgcgc                                                                                               >  1:1851977/1‑150 (MQ=255)
                                                          cTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCAt                                                                                             >  1:4889049/1‑150 (MQ=255)
                                                            taaGGTCTCAGACACCATTAATGCAAAAAATATCTCCATGCGAAAACCAAGCAACTTAACCCTGAGATGCCCGACTACGAGGAGATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTc                                                                                           <  2:1689793/149‑1 (MQ=255)
                                                            gAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTc                                                                                           >  2:4158349/1‑150 (MQ=255)
                                                            gAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTc                                                                                           >  2:2502380/1‑150 (MQ=255)
                                                             aaGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGTGATGCCCGACTACGAGGAGATGAAAACGACCGTGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTcc                                                                                          >  2:6088492/1‑150 (MQ=255)
                                                             aaGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCCTTcc                                                                                          >  2:6263419/1‑150 (MQ=255)
                                                                 tCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGa                                                                                      >  2:779792/1‑150 (MQ=255)
                                                                 tCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGa                                                                                      >  1:4401519/1‑150 (MQ=255)
                                                                 tCGCAGACAGATTGAATGTATCAAATAACTCCTCGCCAAAACCACGCAACTAAACCCTGATATCCCCGACTACGAGGAGTTCAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGa                                                                                      <  2:2846553/150‑1 (MQ=255)
                                                                  cGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGaa                                                                                     >  1:7168882/1‑150 (MQ=255)
                                                                    cAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGa                                                                                   >  2:5620682/1‑150 (MQ=255)
                                                                        cAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCca                                                                               >  2:3421418/1‑150 (MQ=255)
                                                                        cAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCca                                                                               >  1:2169151/1‑150 (MQ=255)
                                                                          gaaTGAATGTATAAAATATCTCCTTGCGAAAACGAAGGAAGGTAACCCTGAGATGCCCGAATACGAGGTGATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCcaca                                                                             <  2:10129011/148‑1 (MQ=255)
                                                                           cctGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAg                                                                            >  2:2240487/3‑150 (MQ=255)
                                                                           cATTAATGTTTCAAATATCGCCTTGAGAAAACGAAGTAACTTAACCCTGCGTTGCCCTACTAGGTGGATATTAAAACGACCGGGATCTTTATGAAAAAATGCCCTGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAg                                                                            <  2:9135602/150‑1 (MQ=255)
                                                                           cATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAg                                                                            >  1:10059059/1‑150 (MQ=255)
                                                                            aTGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAgg                                                                           >  2:7734297/1‑150 (MQ=255)
                                                                              gAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCa                                                                         >  1:5317975/1‑150 (MQ=255)
                                                                              gAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCa                                                                         >  1:8539565/1‑150 (MQ=255)
                                                                                ttGGATCAATTATCTCCATGCTAAAACGATGTAACTTAACCCTTTGATGCCCGACTAGGAGGATTTTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCaaa                                                                       <  2:4091003/149‑1 (MQ=255)
                                                                                    aTCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCg                                                                   >  2:7582362/1‑150 (MQ=255)
                                                                                      aaattatCTCAATGCTAAACCTATGCAACGTAACCCTTAGATGCCCGACTACGTGTATTTGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGtt                                                                 <  1:6626563/146‑1 (MQ=255)
                                                                                         atatCTCCATGCGAAAACGAAGGAACGGAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCGTTCCGCGCATTCCGTGAAGAGCCACAGGCAAACCCGTGGac                                                              >  2:141848/1‑149 (MQ=255)
                                                                                           atCTCCATGCTAAAACGAAGGAACGTAACCCTGATATGCCCGAATACGTGTAGTTTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGaaaa                                                            <  2:6817781/150‑1 (MQ=255)
                                                                                              tcCATGCGAAAACGAAGTAACGTAACCCTGTGCTGCCCGACTAGGTGGTGATTAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCTAACCCGTTGAAAacac                                                         <  2:5174482/150‑1 (MQ=255)
                                                                                               cactGCGAAAACGAAGGAACGTACCCCGGTGATGCCCGACTCCGCGTTGTTTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACAcc                                                        <  2:2412015/147‑1 (MQ=255)
                                                                                                  tGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTc                                                     <  2:6617222/150‑1 (MQ=255)
                                                                                                   gCGAAACCGAAGGACCGTAACCCTGAGATGCCCGACTACGAGGAGATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCg                                                    <  2:3460823/150‑1 (MQ=255)
                                                                                                    cGAAAACGACGTACCGTAACCCTGCGATGCCCGACTCCGAGGATACGAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCTGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgg                                                   <  1:3742044/150‑1 (MQ=255)
                                                                                                     taaaaCGATGCACCGTAACCGTGAGAGGCCCGACTAGGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCggg                                                  <  1:7302263/149‑1 (MQ=255)
                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCggg                                                  >  2:3337767/1‑150 (MQ=255)
                                                                                                     gAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCggg                                                  >  2:696590/1‑150 (MQ=255)
                                                                                                        aaCGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGac                                               >  1:5779586/1‑149 (MQ=255)
                                                                                                        aaCGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGaa                                               >  1:6300055/1‑150 (MQ=255)
                                                                                                           gAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAAt                                            >  2:6473786/1‑150 (MQ=255)
                                                                                                            atgtaaCGTAACCCTGTGAGGCCCTACTACTAGTAGATCAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATc                                           <  1:3001053/146‑1 (MQ=255)
                                                                                                            aaGGACCGTACCCCGGAGATCCCCGACCACGCGGAGATTAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATc                                           <  1:5654183/150‑1 (MQ=255)
                                                                                                            aaGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATc                                           >  1:9462169/1‑150 (MQ=255)
                                                                                                                aaCTTACCCCTTAGAGGCCCGACTACGAGGATATTAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGaaa                                       <  1:2145024/150‑1 (MQ=255)
                                                                                                                aaCTTAACCCTGAGATGCCCGACTACGAGGTTATTAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGaaa                                       <  1:7513281/150‑1 (MQ=255)
                                                                                                                    tAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTa                                   >  2:8261700/1‑150 (MQ=255)
                                                                                                                    tAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTa                                   >  2:7975516/1‑150 (MQ=255)
                                                                                                                        ccTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCt                               <  1:5966139/150‑1 (MQ=255)
                                                                                                                           gtgaggCCCTACTACTTGGTTATCAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGaa                            <  2:934098/145‑1 (MQ=255)
                                                                                                                           ccgagccccGACAAGGAGCAGATGAACACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGaa                            <  1:3495497/144‑1 (MQ=255)
                                                                                                                                 cccGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTg                      >  1:2804214/1‑150 (MQ=255)
                                                                                                                                 cccGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTg                      >  2:5758551/1‑150 (MQ=255)
                                                                                                                                  ccGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTgg                     >  1:657500/1‑150 (MQ=255)
                                                                                                                                     aCTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGa                  >  2:8417209/1‑150 (MQ=255)
                                                                                                                                       tACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAg                >  2:8500215/1‑150 (MQ=255)
                                                                                                                                          gaggagATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGAtt             >  2:6010830/1‑150 (MQ=255)
                                                                                                                                            gtatattaaaaCGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGc           <  1:5134936/143‑1 (MQ=255)
                                                                                                                                            ggagATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGc           >  1:3997095/1‑150 (MQ=255)
                                                                                                                                             tatatcaaaaCGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCa          <  2:4684844/144‑1 (MQ=255)
                                                                                                                                             tagaTGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCa          <  2:2987747/149‑1 (MQ=255)
                                                                                                                                             gttataaaaaCGACCGGGATCTTTAATAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTTAAAACACCTTCGGGGAAAATCTAAATTTATTCTGAACGACTGGCGAAGATTGCa          <  1:8068938/144‑1 (MQ=255)
                                                                                                                                             gagaTGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCa          <  2:5109860/150‑1 (MQ=255)
                                                                                                                                             gagaTGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCa          >  1:1419037/1‑150 (MQ=255)
                                                                                                                                                  taaaaCGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATAc     <  1:8484896/149‑1 (MQ=255)
                                                                                                                                                   aaaaCGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATAcc    >  2:4296673/1‑150 (MQ=255)
                                                                                                                                                   aaaaCGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATAcc    >  2:4934616/1‑150 (MQ=255)
                                                                                                                                                    aaaCGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCt   <  2:1103460/150‑1 (MQ=255)
                                                                                                                                                     aaCGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTAAGGGGAAAATCGAAATTAAATCTGAACGACTGTCGAAGATTGCAGATACCTg  >  1:4597498/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCGAAGATTGCAGATACCTG  >  NC_000913/1662221‑1662519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑