Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,4600GA100.0% 2143.9 / ‑6.7 573V359I (GTC→ATC) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (274/298);  minor base C (1/0);  total (275/298)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 573 total aligned reads displayed.

GAAAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAA  >  NC_000913/2019313‑2019606
                                                                                                                                                      |                                                                                                                                                  
 aaaGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCAtct                                                                                                                                                 <  2:4016006/150‑1 (MQ=255)
 aaaGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCAtct                                                                                                                                                 <  2:6932093/150‑1 (MQ=255)
    gTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCtt                                                                                                                                              <  1:10022615/150‑1 (MQ=255)
    gTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCtt                                                                                                                                              <  2:9062633/150‑1 (MQ=255)
       gCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTAc                                                                                                                                           >  2:8509434/1‑150 (MQ=255)
        cATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                                                                          >  2:3091063/1‑150 (MQ=255)
         aTTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACaa                                                                                                                                         >  1:4166031/1‑150 (MQ=255)
              gTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCg                                                                                                                                    <  1:6778069/150‑1 (MQ=255)
              gTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCg                                                                                                                                    >  1:2260041/1‑150 (MQ=255)
               ttAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgt                                                                                                                                   <  2:3929441/150‑1 (MQ=255)
               ttAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgt                                                                                                                                   >  1:5677177/1‑150 (MQ=255)
                tAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                                                  <  2:5640310/150‑1 (MQ=255)
                tAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                                                  <  2:8560582/150‑1 (MQ=255)
                   ggCAAAGCAACATCAGTGGCGTAAGCTTTAGTGGTCTGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                                               <  1:8961343/150‑1 (MQ=255)
                   ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                                               <  1:5528636/150‑1 (MQ=255)
                   ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                                               >  2:4323929/1‑150 (MQ=255)
                    gCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAAc                                                                                                                              >  2:10248225/1‑150 (MQ=255)
                      aaaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAg                                                                                                                            >  2:5487961/1‑150 (MQ=255)
                      aaaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAg                                                                                                                            <  1:9643694/150‑1 (MQ=255)
                       aaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAgg                                                                                                                           <  1:7843175/150‑1 (MQ=255)
                       aaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAgg                                                                                                                           <  1:6093403/150‑1 (MQ=255)
                            aCATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACa                                                                                                                      >  2:10091112/1‑150 (MQ=255)
                             cATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCTACGCACAGCAAACCAAGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAg                                                                                                                     >  2:8973786/1‑150 (MQ=255)
                             cATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAg                                                                                                                     <  2:7090088/150‑1 (MQ=255)
                               tCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAAAGCACAGCAAACCATGAACCTCGGGCGGGGGAA‑‑‑GACGTCGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAgcg                                                                                                                   >  2:3200153/1‑150 (MQ=255)
                                   tGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGt                                                                                                               >  2:5849342/1‑150 (MQ=255)
                                     gCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTg                                                                                                             >  1:1117688/1‑150 (MQ=255)
                                      cGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                                                                                            <  1:3948131/150‑1 (MQ=255)
                                       gAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTACCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                                                                                           >  1:1782452/1‑150 (MQ=255)
                                          aGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                                                                                           <  2:5041751/150‑1 (MQ=255)
                                          aGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                                                                                        <  2:9404241/150‑1 (MQ=255)
                                             tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                                                                                                     >  1:9191800/1‑150 (MQ=255)
                                             tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                                                                                                     >  1:8044388/1‑150 (MQ=255)
                                              ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCg                                                                                                    <  1:471438/150‑1 (MQ=255)
                                               tAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                                   >  2:2368656/1‑150 (MQ=255)
                                                  tGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTa                                                                                                >  1:602415/1‑150 (MQ=255)
                                                   ggTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                                                                                               <  2:2249540/150‑1 (MQ=255)
                                                   ggTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                                                                                               <  1:2629025/150‑1 (MQ=255)
                                                    gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                                                                                              >  2:3645375/1‑150 (MQ=255)
                                                    gTCAGCAGCAGGCCGCTTCCAAGAAACAGAAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGCTTCCCATATCTTTACAAGTGCGTGTAACAGGCAACAGCGGCGATGATATTTTCGCCTAAc                                                                                              >  2:794618/1‑150 (MQ=255)
                                                     tCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACg                                                                                             >  2:6021064/1‑150 (MQ=255)
                                                         cagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                                                                                         >  1:7328900/1‑150 (MQ=255)
                                                         cagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                                                                                         >  2:6132220/1‑150 (MQ=255)
                                                          agcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                                                                                        <  1:3094649/150‑1 (MQ=255)
                                                            cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg                                                                                      <  1:1286452/150‑1 (MQ=255)
                                                            cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg                                                                                      >  2:3801445/1‑150 (MQ=255)
                                                             agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGt                                                                                     <  2:4496236/150‑1 (MQ=255)
                                                                ccGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGc                                                                                  >  2:7708222/1‑150 (MQ=255)
                                                                 cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCa                                                                                 >  2:6760033/1‑150 (MQ=255)
                                                                 cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCa                                                                                 <  2:174442/150‑1 (MQ=255)
                                                                       ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTaa                                                                           >  2:3298945/1‑150 (MQ=255)
                                                                        cagcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAt                                                                          >  1:827439/1‑150 (MQ=255)
                                                                          gcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc                                                                        <  2:4711292/150‑1 (MQ=255)
                                                                           caacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcg                                                                       >  2:1117128/1‑150 (MQ=255)
                                                                            aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgc                                                                      >  1:2076629/1‑150 (MQ=255)
                                                                            aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgc                                                                      >  1:23630/1‑150 (MQ=255)
                                                                            aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgc                                                                      <  1:3492046/150‑1 (MQ=255)
                                                                            aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgc                                                                      <  2:9933417/150‑1 (MQ=255)
                                                                                  aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCttt                                                                >  1:852745/1‑150 (MQ=255)
                                                                                  aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCttt                                                                >  2:7256505/1‑150 (MQ=255)
                                                                                    aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc                                                              >  1:823355/1‑150 (MQ=255)
                                                                                    aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc                                                              <  1:4323929/150‑1 (MQ=255)
                                                                                        aaCGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCg                                                          >  2:1460828/1‑150 (MQ=255)
                                                                                         aCGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGc                                                         <  1:2876484/150‑1 (MQ=255)
                                                                                           gCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCtt                                                       >  2:8333082/1‑150 (MQ=255)
                                                                                            cacaGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGATCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCttt                                                      >  1:9547212/1‑150 (MQ=255)
                                                                                             acaGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCGTTTCCCCGCTttg                                                     <  1:5029374/150‑1 (MQ=255)
                                                                                               aGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgtt                                                   <  1:10248225/150‑1 (MQ=255)
                                                                                                gCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                  >  1:1402579/1‑150 (MQ=255)
                                                                                                gCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                  >  2:6494927/1‑150 (MQ=255)
                                                                                                gCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                  <  1:9370158/150‑1 (MQ=255)
                                                                                                 cAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTgc                                                 >  1:5910193/1‑150 (MQ=255)
                                                                                                 cAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTATTCCCCGCTTTGTTgc                                                 >  1:9910001/1‑150 (MQ=255)
                                                                                                  aaaCCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTgcg                                                <  2:1292986/150‑1 (MQ=255)
                                                                                                  aaaCCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTgcg                                                <  1:7519358/150‑1 (MQ=255)
                                                                                                     ccATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTc                                             >  1:336040/1‑150 (MQ=255)
                                                                                                        tGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTGTGGTGCGCTCAAg                                          >  2:5207169/1‑150 (MQ=255)
                                                                                                          aaCCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGAc                                        <  2:9177024/150‑1 (MQ=255)
                                                                                                             ctctGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCa                                     <  1:9772659/150‑1 (MQ=255)
                                                                                                               ctGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAgg                                   <  2:9757873/150‑1 (MQ=255)
                                                                                                                tGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGa                                  >  1:6993813/1‑150 (MQ=255)
                                                                                                                  gCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTGTGTTGCGCTCAAGACGCAGGATa                                >  1:331757/1‑150 (MQ=255)
                                                                                                                   cGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATaa                               <  2:8359903/150‑1 (MQ=255)
                                                                                                                    gggggAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAAt                              >  2:8162364/1‑150 (MQ=255)
                                                                                                                      gggAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTa                            <  1:6603035/150‑1 (MQ=255)
                                                                                                                        gAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGc                          <  1:6876357/150‑1 (MQ=255)
                                                                                                                        gAA‑‑‑GACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGc                          <  1:7232082/150‑1 (MQ=255)
                                                                                                                                 gacgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATaa                    <  1:6348490/150‑1 (MQ=255)
                                                                                                                                 gacgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATaa                    >  1:868479/1‑150 (MQ=255)
                                                                                                                                   cgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGc                  <  1:2538206/150‑1 (MQ=255)
                                                                                                                                       aCGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTa              <  2:7465959/150‑1 (MQ=255)
                                                                                                                                       aCGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTa              >  2:1833893/1‑150 (MQ=255)
                                                                                                                                         gCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGc            <  2:8750449/150‑1 (MQ=255)
                                                                                                                                           ttCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTGGATATTTTCGCCTAACGTCAGAGGGAGCACCGTAATCCGCGTCTTTTCCCCGCGTTGTTGCGCTCAAGACGCCGGCTAATTAGCCGATAAGCAGTAGCgc          >  1:3801916/1‑149 (MQ=255)
                                                                                                                                               ggTTCCCATCTCTTTACACGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGAcaca      <  1:8877916/150‑1 (MQ=255)
                                                                                                                                               ggTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGAcaca      <  1:5240846/150‑1 (MQ=255)
                                                                                                                                               ggTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGAcaca      <  2:9604908/150‑1 (MQ=255)
                                                                                                                                                   cccATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTCGCCGATAAGCAGTAGCGACACAGGaa  <  1:4372634/150‑1 (MQ=255)
                                                                                                                                                   cccATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACCGGaa  >  2:2685518/1‑150 (MQ=255)
                                                                                                                                                   cccATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGaa  >  1:7101300/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GAAAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAA‑‑‑GACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGAA  >  NC_000913/2019313‑2019606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑