Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,228 C→A 14.4% intergenic (‑24/+284) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2280CA14.4% 917.7 / 27.0 418intergenic (‑24/+284)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (138/188);  new base A (71/3);  total (226/192)
Fisher's exact test for biased strand distribution p-value = 1.72e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 427 total aligned reads displayed.

ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCG  >  NC_000913/2177079‑2177376
                                                                                                                                                     |                                                                                                                                                     
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGTAACCGCATAACCTCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc                                                                                                                                                       <  1:8410117/150‑1 (MQ=255)
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc                                                                                                                                                       <  2:5736429/150‑1 (MQ=255)
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGGTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTc                                                                                                                                                       >  1:9890727/1‑150 (MQ=255)
 tGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCg                                                                                                                                                      <  1:5837621/150‑1 (MQ=255)
 tGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCg                                                                                                                                                      <  1:96255/150‑1 (MQ=255)
  gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGa                                                                                                                                                     <  2:6360938/150‑1 (MQ=255)
   cAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAt                                                                                                                                                    <  2:6827459/150‑1 (MQ=255)
       ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                <  1:4189885/150‑1 (MQ=255)
         ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                              <  2:2349182/150‑1 (MQ=255)
                gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAt                                                                                                                                       >  1:1371075/1‑150 (MQ=255)
                gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAt                                                                                                                                       >  1:1683268/1‑150 (MQ=255)
                gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAt                                                                                                                                       <  2:2820355/150‑1 (MQ=255)
                 tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATg                                                                                                                                      >  1:5059992/1‑150 (MQ=255)
                 tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATATAAtt                                                                                                                                      >  1:4757955/1‑149 (MQ=255)
                    cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                   <  2:1269852/150‑1 (MQ=255)
                    cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATGaaa                                                                                                                                   >  2:2420488/1‑150 (MQ=255)
                     tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGaaaa                                                                                                                                  >  2:3608890/1‑149 (MQ=255)
                     tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAAAATAAAGAAat                                                                                                                                  >  1:4434753/1‑150 (MQ=255)
                      accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                 <  1:516766/150‑1 (MQ=255)
                         accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                              <  2:6229990/150‑1 (MQ=255)
                          ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                             <  2:8329396/150‑1 (MQ=255)
                              ttGCATCGTTTCGAGATTGTGGATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATtttttt                                                                                                                         >  1:6516497/1‑150 (MQ=255)
                              ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATTTGttt                                                                                                                         >  1:10095793/1‑150 (MQ=255)
                               tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                        <  1:1005594/150‑1 (MQ=255)
                                gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTGttttt                                                                                                                       >  2:4224685/1‑150 (MQ=255)
                                 cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATTAAAATATTTGTTTTTa                                                                                                                      >  1:8596448/1‑150 (MQ=255)
                                    cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAat                                                                                                                   <  2:8213646/150‑1 (MQ=255)
                                     gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAAAAAAATGAAAATAATTTTTTTTAAata                                                                                                                  >  2:2003510/1‑150 (MQ=255)
                                      tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                 <  2:507967/150‑1 (MQ=255)
                                       ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                <  1:6855431/150‑1 (MQ=255)
                                        tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAATAAAATATTTTTTTTTAAATATCg                                                                                                               >  2:8680843/1‑150 (MQ=255)
                                         cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                              >  1:5943992/1‑150 (MQ=255)
                                          gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTACATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAAAGAAAAAATTTTTTTTTTAAAATCgag                                                                                                             >  1:6149130/1‑150 (MQ=255)
                                             aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATa                                                                                                          >  1:7489245/1‑150 (MQ=255)
                                               tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAAc                                                                                                        >  1:2356401/1‑150 (MQ=255)
                                                 tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGATATAACGa                                                                                                      >  2:7860166/1‑150 (MQ=255)
                                                       ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATTAAATTATTTTTTTTTAAATATCGAGTTAAAGCTCACaa                                                                                               >  1:4025169/1‑150 (MQ=255)
                                                        tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGTAATTATTTGTTTTTAAATATAGAGATAAAGATCACaaa                                                                                              >  1:8819476/1‑150 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                            <  1:3516185/150‑1 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                            <  1:19153/150‑1 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                            >  2:4924012/1‑150 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGGTTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACaaaaa                                                                                            >  1:5718973/1‑150 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAATTAAAATATTTTTTTTTAAAAAAAGAGATAACGAACACaaaaa                                                                                            >  2:1847321/1‑150 (MQ=255)
                                                          aaTGCCGGAACCGCATAAACGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                            >  2:5388247/1‑150 (MQ=255)
                                                           aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                           <  2:2930759/150‑1 (MQ=255)
                                                              ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                        <  2:8441795/150‑1 (MQ=255)
                                                               cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATAGAGATAACGATCACACAATCGACa                                                                                       >  2:8112081/1‑150 (MQ=255)
                                                               cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTAATTTTTTTTTAAAATAGAGATAAAGAACACAAAAAccaaa                                                                                       >  1:9264898/1‑146 (MQ=255)
                                                               cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTGTCATATCCTGTCGTGTGTTTTCGGTTTCAAAATATAATGAAATTATTTGTTTTTGAATATCGAGATAACGATCACCACAACGAcc                                                                                       >  1:8227456/1‑149 (MQ=255)
                                                                    ccGCAGAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAATAAAATATTTTTTTTTAAATAACGAGAAAAAGATAACAAAAAAGACAAAATg                                                                                  >  2:2641475/1‑150 (MQ=255)
                                                                       cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                               <  2:7540292/150‑1 (MQ=255)
                                                                              gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                        <  2:4333475/150‑1 (MQ=255)
                                                                              gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAAATATTTTTTTTTTAATATAGGGATAAAGATCAAAAAAAAGAAAAAATGAAAattttta                                                                        >  1:7739666/1‑149 (MQ=255)
                                                                                cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                      <  2:4724470/150‑1 (MQ=255)
                                                                                cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAACCACAATAATAAAATTATTCga                                                                      >  2:10073968/1‑150 (MQ=255)
                                                                                   gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                   <  2:5160558/150‑1 (MQ=255)
                                                                                    cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTAgaggag                                                                  >  2:7057159/1‑150 (MQ=255)
                                                                                     tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGATTTAAAAAAAAAAAAAAAAAAATTGTTTTTAAAAATAGAGAGAAAGATCAAAAAAAAGACAAAATTAAAATTATAACAGGAGt                                                                 >  1:3280741/1‑150 (MQ=255)
                                                                                       tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATAAAAAGAAAATAATTTTTTTTTAATATAGAGAAAAAGATAACAAAAAACACAAAATTAAAAATATTAGTGGAGTgg                                                               >  1:2738773/1‑149 (MQ=255)
                                                                                         cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAATAAAATAATTTTTTTTTAATAAATAGAGAAAGCTCACAAAAAAGACAAAAATAAAAATATTAGTGGGGTGtaa                                                             >  1:10213090/1‑150 (MQ=255)
                                                                                                cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTAAATTATTTTTTTTTTAATAAATATATAAAGAAAAAAAAAAACAAAAAAATAAAATTATTATAGGAGTTTAAGGCaaaa                                                      >  1:1445352/1‑150 (MQ=255)
                                                                                                 aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTAAAAAAAAAAATAAAAAATTTTTTTTTTAATATAGAGATAAACAAAACAAAAAAGACAAAATTAAAAAAAATATAGGAGTGAAAGGCaaaaa                                                     >  1:2090036/1‑150 (MQ=255)
                                                                                                    accTGCTTTGCCGACACCCCCACAATTTTAAGATCCTGTCGTTTTTTTTCGATTAAAAAATATACTTAAATTATTTGTTTTTAATTATCGATATAACGAACACAATCACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                  <  1:8170464/148‑1 (MQ=255)
                                                                                                    aTCTGCTTTGTCGATACCACGTACATTTTCGTATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                  <  2:8869861/150‑1 (MQ=255)
                                                                                                    aTCTGATTTGTCGATACCACGTACTTTTTCATATCCTGTCGTTTTTTTTCGTTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAATTTATTCGAGGAGTGAAAGGCAAAAAAAc                                                  <  2:7458328/150‑1 (MQ=255)
                                                                                                      cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                <  2:6005682/150‑1 (MQ=255)
                                                                                                       ttcTTTGTCTATACAACTTACATTTTAATATCCTGTCGTTTTTTTTCGATTTAAAAATATATTTAAATTTTTTTTTTTTAAATTTCGATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                               <  2:1019266/148‑1 (MQ=255)
                                                                                                               ctacaCCACTTAATTTTTAATTTCCTGTTTTTTTTTTTCTTTTTAAAAATATATTTAATTTTTTTTTTTTTAAATATCGATATAACTATCAAAAAAACGACAATATTAAATTTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                       <  1:7339481/146‑1 (MQ=255)
                                                                                                                gATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                      <  2:6096866/150‑1 (MQ=255)
                                                                                                                gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTGAAAATATAATGAAAATATTTGTTTTTAAATATAGATATAAAGATCACAAAAAAGACAATATTAAAATTATTAGAGGAGTGAAAGGCAAAAAAACGGCCACCCGaga                                      >  2:7171280/1‑148 (MQ=255)
                                                                                                                 aTACCACTTACATTTTCATATCCTGTCTTTTTTTTACTATTACAAAATATATTGAATTTATTTTTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                     <  1:5641210/150‑1 (MQ=255)
                                                                                                                 aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTACAAAATATAATTAAATTTTTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                     <  2:3520919/150‑1 (MQ=255)
                                                                                                                   aCCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTTAATTATTTGTTTTTAAAAATCGTGATAACGATCACAAAAACGACAAAATGGAAATTATTCGAGGGGGGTAAGGCAAAAAAACGGCCTCCCGCGAggg                                   >  2:9765131/1‑150 (MQ=255)
                                                                                                                    ccACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATAGAGATAACGCTCACAAAAACGAAAATATGAAAATTATTCGAGGAGTGTAAGGCAAAAAAACGGCCTCCCGCTAGGGa                                  >  2:9652223/1‑150 (MQ=255)
                                                                                                                     cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                 <  2:3109398/150‑1 (MQ=255)
                                                                                                                        gTACATTTTCATACCCTGTCGTTTGTTTTCTATTCCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                              <  1:8468173/150‑1 (MQ=255)
                                                                                                                          aCATTTTCATATCCTGTCGTTTGTTTCCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt                            <  1:6724376/150‑1 (MQ=255)
                                                                                                                            aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                          <  2:4471653/150‑1 (MQ=255)
                                                                                                                             ttttCATTTCCTGGCGTTTGTTTTCTTTTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAACGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGc                         <  1:4875435/150‑1 (MQ=255)
                                                                                                                             ttttCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACACAAACGACAAAATGAAAATTATTCGCGGAGTGAAAGGCAAAAAAACCGCCGCCCGATAGGGAACACGTAGc                         >  2:162827/1‑150 (MQ=255)
                                                                                                                                tCATATCTTGTTTTTTTTTTTCTATTTCAAAATATATTTAATTTTTTTTTTTTTAAATTTCTTGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                      <  1:1101990/150‑1 (MQ=255)
                                                                                                                                tCATATCCTGTCGTTTTTTTTCGATTACAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                      <  2:8638567/150‑1 (MQ=255)
                                                                                                                                tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                      <  2:6599453/150‑1 (MQ=255)
                                                                                                                                tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                      <  2:3247492/150‑1 (MQ=255)
                                                                                                                                     tCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                  >  2:6457101/1‑150 (MQ=255)
                                                                                                                                      ccttttgtttgtttTCTATTAAAAAATATAATTAATTTTTTTTTTTTAAAATATCTTTATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                <  1:3731680/144‑1 (MQ=255)
                                                                                                                                      ccTGTCTTTTTTTTACTATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGTGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                <  1:7071541/150‑1 (MQ=255)
                                                                                                                                      ccTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                <  2:931593/150‑1 (MQ=255)
                                                                                                                                      ccTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                <  2:9401488/150‑1 (MQ=255)
                                                                                                                                      ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                <  2:9239971/150‑1 (MQ=255)
                                                                                                                                           cGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATTAAAATTAATCGAGGAGTGTAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt           >  1:7979486/1‑150 (MQ=255)
                                                                                                                                             tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGTAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGtttt         >  1:9092169/1‑150 (MQ=255)
                                                                                                                                             tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt         >  2:4765487/1‑150 (MQ=255)
                                                                                                                                               tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa       <  1:4262924/150‑1 (MQ=255)
                                                                                                                                                 ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATAACGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg     >  2:2153294/1‑150 (MQ=255)
                                                                                                                                                  tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTATCAAAGTGCGCGTGTTTTTATGc    <  2:1403398/150‑1 (MQ=255)
                                                                                                                                                  tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc    >  2:7625877/1‑150 (MQ=255)
                                                                                                                                                  tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTATGcc   >  2:3940262/1‑150 (MQ=255)
                                                                                                                                                   ttCTATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc   <  1:2226617/150‑1 (MQ=255)
                                                                                                                                                    tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg  >  2:2709425/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCG  >  NC_000913/2177079‑2177376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: