Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,688 A→G 9.9% intergenic (+295/+420) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,6880AG 9.9% 561.4 / 45.0 265intergenic (+295/+420)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (82/131);  new base G (6/19);  total (90/175)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

ACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATC  >  NC_000913/2304539‑2304837
                                                                                                                                                     |                                                                                                                                                     
aCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGGTACGCCGCATCCGACCGCGCACGCCCa                                                                                                                                                       >  1:8435223/1‑150 (MQ=11)
 cAAGGCGGACACGCCGCATCCGACATACAACGCCCGAGCCGTTTGCCTGATGCTACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                                                                      <  1:6517864/150‑1 (MQ=11)
   cggCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    <  1:3449748/149‑1 (MQ=21)
   aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                                    <  2:2473524/150‑1 (MQ=34)
     gaggTCACGCCGCATCGGTCAGCCATCGCCCGTGCCGTTTGCGTGATTGGACGGTGTCTCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                                  <  2:9291953/148‑1 (MQ=11)
     gCGGTCACGCCGCTTCCGACATCCATCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                                  <  2:33494/150‑1 (MQ=21)
     gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                                  <  1:9531391/150‑1 (MQ=34)
     gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                                  <  1:6765618/150‑1 (MQ=34)
      cGGTCACGCCGCATCCGACATCCAACGCCCGAGCGGGTTGCCTGTTGCGACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGATGTGCTCCACCCCGTTGGTCGGATACGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                                                 <  1:3825591/150‑1 (MQ=11)
       ggTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGt                                                                                                                                                <  1:229051/150‑1 (MQ=34)
       ggTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGt                                                                                                                                                <  1:1537687/150‑1 (MQ=34)
        gTCACGCCGCATCCTACATCCAACGACCGAGCCGGTTGACTTATGCGAAGATTGCGAGTCTTAACAGTCCTACACCTCTGTGAAGTGCTACAACCCGTAGGTCTGATAAGGCTGTTACGCCGCATCCGACATCTAACGCCAAAGCCTGtt                                                                                                                                               <  2:3140312/150‑1 (MQ=11)
        gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                               <  1:573156/150‑1 (MQ=34)
        gTCACGCAGCATCCGACATCCAACGCGCGAGGCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                               <  2:8212353/150‑1 (MQ=255)
          cACGCCGCCTCCGACATCCCACGCCCTAGCCGGTTGCCCGAAGCGACGCTGGCGCGGCTTATCAGGCCCACAGCGCCGTGACGTGCACCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCTACATCCAACGCCCGAGCCGGTTGc                                                                                                                                             <  2:7485218/150‑1 (MQ=11)
            cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                           <  1:3501271/150‑1 (MQ=32)
            cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                           <  2:6368975/150‑1 (MQ=32)
               cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                        <  1:9027663/150‑1 (MQ=32)
                gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  1:8472019/150‑1 (MQ=32)
                gcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  2:9707928/150‑1 (MQ=32)
                acATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                       <  2:4060425/149‑1 (MQ=17)
                 cATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGAGAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGCTGcagcgagc                                                                                                                                      >  2:7146281/1‑143 (MQ=11)
                  aTCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                             >  1:5002889/1‑142 (MQ=32)
                  aTCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                             <  2:5002889/142‑1 (MQ=32)
                   tCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGGTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGa                                                                                                                                    >  1:6354534/1‑150 (MQ=17)
                     cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGCCGCGGGCGCGGCGTATCAGGCCGCCACCCCGGTGAACTGCGCCACCCCCGAGGGCGGCTAAGGCGGGGACGCCGCCACCCACCGCGAACCCCCCCGCCGGGTGCCGGCTGCGACg                                                                                                                                  >  1:7080277/1‑150 (MQ=2)
                     cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACg                                                                                                                                  >  2:3601388/1‑150 (MQ=32)
                      gACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGc                                                                                                                                 >  2:1504602/1‑150 (MQ=32)
                         aTCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                              <  1:3700290/150‑1 (MQ=32)
                         aTCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCGACACCGCTGGGAAGTGCTCCACCCCGTAGGGCGGATAAGGCGGTTACGCCGCATCCGACCTCGAACGCCCAAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                              >  1:8689800/1‑150 (MQ=11)
                          tCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                             <  2:783823/150‑1 (MQ=32)
                          tCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                             <  1:6837806/150‑1 (MQ=32)
                            cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                           <  1:4798872/150‑1 (MQ=32)
                             aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                          <  2:1143154/150‑1 (MQ=17)
                             aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATAAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                          >  1:9249560/1‑150 (MQ=17)
                              aCGCCCGAGCGGTTTGCCTGATGCGACTCTGTCGAGTCTTATCAGGGCTACACCGCGGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                         <  1:5390113/150‑1 (MQ=11)
                               cGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                        <  2:38497/150‑1 (MQ=32)
                               cGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                        <  1:1145672/150‑1 (MQ=32)
                               atcccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                        <  2:8777950/148‑1 (MQ=11)
                                gCCCGAGCCGGTTGCCTGCTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCCGACAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                                       <  1:8016644/150‑1 (MQ=9)
                                gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                                       <  2:7688782/150‑1 (MQ=31)
                                gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                                       <  2:3807013/150‑1 (MQ=31)
                                gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                                       <  1:9836541/150‑1 (MQ=31)
                                gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                                       <  2:2118849/150‑1 (MQ=31)
                                 cccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                                      <  1:4663353/150‑1 (MQ=11)
                                 cccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                                      <  1:5818418/150‑1 (MQ=11)
                                                                                                   aCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCg                                                    >  1:9898142/1‑150 (MQ=11)
                                                                                                    ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                                   <  1:7773085/150‑1 (MQ=31)
                                                                                                    ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                                   >  1:5950468/1‑150 (MQ=31)
                                                                                                    ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATCCGACGATGGCGCGTCTTGTCAGGACTACGCAGCTGTGATGAGCTCCACCCCGTCGGTTGGAGAAGGCGGTTACGCCGCGTCCgg                                                   >  2:4486002/1‑149 (MQ=11)
                                                                                                    ccccGTAGGTCGGAGCAGGCGGTTACGCCGCATCCGACATCGAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                                   <  2:2867012/150‑1 (MQ=9)
                                                                                                      ccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGGAGTGCTCGACCCCGTAGGGTGGCTCTGGCGGTTCCGCCGCATCCgcgg                                                 >  2:4120449/1‑147 (MQ=11)
                                                                                                       cGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACAt                                                <  1:5192318/150‑1 (MQ=32)
                                                                                                           ggTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCaa                                            <  2:1902601/150‑1 (MQ=34)
                                                                                                           ggTCCGATAAGGCGGTTACGCCGCAGCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCaa                                            <  1:8902085/150‑1 (MQ=255)
                                                                                                            gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                           <  2:6036944/150‑1 (MQ=34)
                                                                                                            ctctgATTAGTCGGTTTCTCCGCATCGGACATTTAAGTTCTGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTTGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                           <  1:9180491/146‑1 (MQ=11)
                                                                                                             tCGGATTCGGCGGTGACGCCGCAGCCGACATCTAACGCCCACGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCTCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg                                          <  1:8980277/150‑1 (MQ=11)
                                                                                                             tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg                                          <  1:5331925/150‑1 (MQ=34)
                                                                                                             tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg                                          >  2:5332071/1‑150 (MQ=34)
                                                                                                                gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGAcc                                       >  2:3735347/1‑150 (MQ=17)
                                                                                                                  tAAGGCGGTGACGCCGCATCCGACATCCAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                     <  2:5555446/150‑1 (MQ=255)
                                                                                                                  cacggcggcGACGCCGCATCCGACATCCACCGCCCTAGCCGGTTGCCTGATGCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                     <  2:5757181/147‑1 (MQ=11)
                                                                                                                   aaGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAg                                    <  2:8994035/150‑1 (MQ=37)
                                                                                                                    aGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTGGGAGCAGGCGGTTCCGCCGCATCCGCCAGCCACCGTCCgcgc                                   >  1:408961/1‑150 (MQ=11)
                                                                                                                      gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCg                                 >  1:3784280/1‑150 (MQ=17)
                                                                                                                      gCGGTCACGCCGCATCCGACATCAAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                 <  1:7688720/150‑1 (MQ=255)
                                                                                                                          ttACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTg                             >  2:424279/1‑150 (MQ=21)
                                                                                                                          ttACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGATCCACCCCGTAGGTAGGGTACGGCCGATACGCCGCATCCGACATCCAACGTCAGCGCCGGTTg                             >  2:2590827/1‑150 (MQ=11)
                                                                                                                           tACGCCGGATCCGTCATCTCCCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                            <  1:6062881/150‑1 (MQ=11)
                                                                                                                           tACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                            <  2:10234863/150‑1 (MQ=37)
                                                                                                                           ctcgcctcATCCGTCTTCTAACGCCCATGCCGGTTGCCTGATTCGACGCTGGCGCGTCTTATCCGGCCTACACCGCTGTGATGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCTTCCTACATCCAACGTCCTAGCCGGTTGc                            <  1:10127262/148‑1 (MQ=11)
                                                                                                                            aCGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGct                           >  2:7260575/1‑149 (MQ=37)
                                                                                                                              gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCTAAGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTg                         <  1:9726778/150‑1 (MQ=255)
                                                                                                                              gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTg                         >  1:4258625/1‑150 (MQ=37)
                                                                                                                               ccgcATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTATTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                        <  1:3251682/150‑1 (MQ=255)
                                                                                                                               ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                        <  2:9405894/150‑1 (MQ=37)
                                                                                                                               ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGGAGGGTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                        >  1:8410375/1‑150 (MQ=255)
                                                                                                                                cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAt                       <  2:4553894/150‑1 (MQ=21)
                                                                                                                                 gcATCCGACATCCAAGGCCGATGCGGGTGGCCTGATGCTACGCTGGCGCGTCGTATGAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATg                      <  2:6682615/150‑1 (MQ=11)
                                                                                                                                      cGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACg                 >  2:9510480/1‑150 (MQ=21)
                                                                                                                                        tcATCCAACGCCCTCGCCGGTTGCCTGATGCGACGCGTGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCt               <  2:6948040/149‑1 (MQ=11)
                                                                                                                                          aTCTACCGCCAAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTgg             <  2:6546542/150‑1 (MQ=255)
                                                                                                                                          aTCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTgg             >  2:52006/1‑150 (MQ=17)
                                                                                                                                             tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc          >  2:2295038/1‑150 (MQ=37)
                                                                                                                                             tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc          <  2:8689800/150‑1 (MQ=37)
                                                                                                                                             caaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc          <  1:9398380/149‑1 (MQ=25)
                                                                                                                                              aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGcc                           <  1:1914984/132‑1 (MQ=37)
                                                                                                                                              aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg         >  2:592856/1‑150 (MQ=37)
                                                                                                                                              aaCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg         >  2:10027834/1‑150 (MQ=37)
                                                                                                                                              aaCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg         >  1:7443014/1‑150 (MQ=37)
                                                                                                                                               ccGCCCCCGCCGGTGGCCGGCTGCGACGCTGGCGCGTCTTCTCAGGCCTACCCCGCTGTGACGTGCTCCCCCCCGGAGGTGGGATAAGGCGGTTACGCCGCCTCCGACATCCAACGGCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt        <  2:7080277/149‑1 (MQ=11)
                                                                                                                                               aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGCTAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt        >  2:3649059/1‑150 (MQ=37)
                                                                                                                                               aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGc                                   >  2:8558940/1‑123 (MQ=38)
                                                                                                                                                 gCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt      >  2:7744164/1‑150 (MQ=37)
                                                                                                                                                 gCCCAAGCCGGTTGCCAGATGCGACGCTGGCGCGTCTTATCAGGCCAACACCGCTGTGAATTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACCCTGGCGCGTCt      >  1:244102/1‑150 (MQ=255)
                                                                                                                                                    cAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt   <  1:2920027/150‑1 (MQ=37)
                                                                                                                                                    cAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt   <  1:2920154/150‑1 (MQ=37)
                                                                                                                                                    cAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTAACGCCGCATCCCACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt   >  2:1180302/1‑150 (MQ=255)
                                                                                                                                                     aaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATAAGGCCAACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCAACCGACATCCAACCACCGAGCCGGTTGCCTGATGCGAAGCTGGCGCGTCTTATc  >  1:7118284/1‑150 (MQ=11)
                                                                                                                                                     |                                                                                                                                                     
ACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATC  >  NC_000913/2304539‑2304837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: