Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,305,085 A→G 6.1% intergenic (+692/+23) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,305,0850AG 6.1% 1908.1 / 74.0 596intergenic (+692/+23)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (269/287);  new base G (17/19);  total (288/308)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01

Only 100 of 601 total aligned reads displayed.

CTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTC  >  NC_000913/2304936‑2305233
                                                                                                                                                     |                                                                                                                                                    
 tGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTTCTCCACCCCGTAGGTTGGATAAGTCGTTTACGCATGATCCTACTTCTAACGCCCTTGCCGGTGGGATGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATAc                                                                                                                                                     <  1:4970730/150‑1 (MQ=17)
 gccagcgTCTTCTCAGGCATACACCGCGGTGAAGTGCTCCACCCCGGAGGTTGGATAGGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCGGATGCGACGCTGGCGCGTCGTATCAGCATACGCCGCAGCCGGCATAc                                                                                                                                                     >  2:7814705/5‑150 (MQ=255)
       gTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGcc                                                                                                                                               <  2:9465915/150‑1 (MQ=255)
        tCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCg                                                                                                                                              <  2:598573/150‑1 (MQ=255)
        tCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCg                                                                                                                                              >  1:98845/1‑150 (MQ=255)
        tCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCg                                                                                                                                              <  2:1492614/150‑1 (MQ=255)
         cTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCgg                                                                                                                                             <  1:1676243/150‑1 (MQ=255)
          ttATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGa                                                                                                                                            <  1:1789486/150‑1 (MQ=255)
          ttATCAGGCCTACAACGCTGTGAAGTGCTCCACCCCGTAGGTGGGATAAGGCGGTTACGCCGCATCCTACATCTAACGCCCGAGCCTGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGa                                                                                                                                            <  1:7455561/150‑1 (MQ=17)
            aTCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAtg                                                                                                                                          >  2:3212026/1‑150 (MQ=255)
              cAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGAtgtg                                                                                                                                        <  2:4310824/150‑1 (MQ=255)
              cAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGAtgtg                                                                                                                                        <  2:8441852/150‑1 (MQ=255)
              cAGGCCTACACCGCGGTGATGTGCTCCACCCCGTTGTTTGGATTGGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGAtgtg                                                                                                                                        <  1:4885039/150‑1 (MQ=17)
               aGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTgg                                                                                                                                       >  1:3655532/1‑150 (MQ=255)
                 gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATCCGCCACATCCGGCATACCATGCCGGATGTGGCg                                                                                                                                     >  2:9219364/1‑150 (MQ=255)
                   cTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTa                                                                                                                                   >  2:8915907/1‑150 (MQ=255)
                      cacCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCa                                                                                                                                <  1:7388502/150‑1 (MQ=255)
                       acagCTTTGAACTGCTCCACACCGTAGGTTGGATAAGGCGGTTACGACGCATCAAACATCTAACGCCCTAGCCGCTTTACAGATGCGACGCTGGCGCGACTAATAATCATACACCACAACCGTCATACCAAGCCGCATGTGGCGGATCaa                                                                                                                               >  2:4824675/4‑149 (MQ=12)
                       acCGCTGTGAAGTGCGCCACCCCGTAGGTTGGATACGGCGGATCCGCCGCATCCCACATCTACCGCCCGAGCCGGTTGCCTGATGCGCCGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCAt                                                                                                                               <  2:6807749/150‑1 (MQ=21)
                        ccGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCAtt                                                                                                                              <  1:2109242/150‑1 (MQ=255)
                         cGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTa                                                                                                                             >  2:10193217/1‑150 (MQ=255)
                                     cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATc                                                                                                                 <  2:7259541/150‑1 (MQ=255)
                                      tCCACCCCGTAGGTTTGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATcc                                                                                                                <  1:3835039/150‑1 (MQ=255)
                                      tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCATCATCTTACGCCCGAGCCGGTTGCCTGATTCGACGCTGGGGCGTCTTATCAGCATACGCCACATCCGGCTTACCATGCCTGATGTGGCGTATCATTACAACGCAATATcc                                                                                                                <  2:1283060/150‑1 (MQ=25)
                                        cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGc                                                                                                              >  1:8399799/1‑150 (MQ=255)
                                         aCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGcc                                                                                                             >  1:1432413/1‑150 (MQ=255)
                                           cccGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCAc                                                                                                           >  1:7003654/1‑150 (MQ=255)
                                            ccGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCAct                                                                                                          >  2:2968643/1‑150 (MQ=255)
                                            ccGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCAct                                                                                                          >  2:1799156/1‑150 (MQ=255)
                                             cGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCActt                                                                                                         >  1:6654187/1‑150 (MQ=255)
                                                   ttGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATTTCCGCCACTTCttttt                                                                                                   >  1:730415/1‑150 (MQ=255)
                                                   ttGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCATGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCACTATCCGCCACTTCttttt                                                                                                   >  1:8606837/1‑150 (MQ=255)
                                                    tGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTg                                                                                                  <  1:8838783/150‑1 (MQ=255)
                                                    tGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACGTCTTTTTg                                                                                                  >  1:5399779/1‑150 (MQ=255)
                                                      gATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGaa                                                                                                >  2:4743983/1‑150 (MQ=255)
                                                       aTAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAAc                                                                                               >  1:4527103/1‑150 (MQ=255)
                                                       aTAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGGATCATTACAACGCAATATCCGCCACTTCTTTTTGAAc                                                                                               >  2:2241277/1‑150 (MQ=255)
                                                          aGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACggg                                                                                            >  2:2142966/1‑150 (MQ=255)
                                                          aGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACggg                                                                                            >  1:8301716/1‑150 (MQ=255)
                                                            gCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGcc                                                                                                                                               <  2:3783100/97‑1 (MQ=255)
                                                            gCGGTTACGCCGCATCCAACATCTAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCAGACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGtt                                                                                          >  1:6487131/1‑150 (MQ=255)
                                                             cGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTg                                                                                         <  1:10022493/150‑1 (MQ=255)
                                                              ggTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGc                                                                                        <  1:3485065/150‑1 (MQ=255)
                                                                ttACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCgg                                                                                      <  1:9066836/150‑1 (MQ=255)
                                                                 tACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGt                                                                                     <  1:1260364/150‑1 (MQ=255)
                                                                 tACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGt                                                                                     <  2:8587651/150‑1 (MQ=255)
                                                                   ctccgcATCCTACATCTTACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGGTGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGttt                                                                                   <  1:5575234/148‑1 (MQ=255)
                                                                   cgccgcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGGTGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGttt                                                                                   <  2:2374862/150‑1 (MQ=255)
                                                                     ccgcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCa                                                                                 >  2:969406/1‑150 (MQ=255)
                                                                     ccgcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAAAATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCa                                                                                 >  1:102937/1‑150 (MQ=255)
                                                                      cgcATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCaa                                                                                                                     >  2:417264/1‑113 (MQ=255)
                                                                          tCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGc                                                                            >  1:6176311/1‑150 (MQ=255)
                                                                          tCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAAAGGGTTGCGGTTTCAACTGc                                                                            >  1:5904214/1‑150 (MQ=255)
                                                                           cctaCATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCg                                                                           <  2:3441639/147‑1 (MQ=255)
                                                                            cAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCgg                                                                          >  2:9579829/1‑150 (MQ=255)
                                                                            cAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCgg                                                                          <  2:8677442/150‑1 (MQ=255)
                                                                             aaCATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGt                                                                         <  1:5251626/150‑1 (MQ=255)
                                                                                aTCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCAGGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTc                                                                      >  1:3777270/1‑150 (MQ=255)
                                                                                  cTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCgg                                                                    >  1:692407/1‑150 (MQ=255)
                                                                                   tAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGc                                                                   <  1:57003/150‑1 (MQ=255)
                                                                                    accGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCg                                                                  <  1:6452868/148‑1 (MQ=255)
                                                                                          cGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTAt                                                            <  1:923034/150‑1 (MQ=255)
                                                                                           gAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACAACCGGCATACCATGCCGGATGTGGCGTATCATTAAAACGCAAAATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATc                                                           >  1:1562416/1‑150 (MQ=255)
                                                                                              ccGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATctgc                                                        >  2:5127262/1‑150 (MQ=255)
                                                                                                 gTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCtgctgc                                                     >  2:9048076/1‑150 (MQ=255)
                                                                                                  ttGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCACTATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACGATCTGctgcc                                                    >  1:2486352/1‑150 (MQ=255)
                                                                                                      cTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATAGCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCg                                                <  1:2303723/150‑1 (MQ=255)
                                                                                                       tGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCgg                                               <  1:4184170/150‑1 (MQ=255)
                                                                                                       tGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCgg                                               <  1:9677608/150‑1 (MQ=255)
                                                                                                        gATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGc                                              >  2:8316678/1‑150 (MQ=255)
                                                                                                          tGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCtt                                            <  1:268918/150‑1 (MQ=255)
                                                                                                              aCGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCACCTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCg                                        <  2:7057643/150‑1 (MQ=255)
                                                                                                              aCGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCg                                        <  2:8044772/150‑1 (MQ=255)
                                                                                                              aCGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCg                                        >  1:3286612/1‑150 (MQ=255)
                                                                                                                gCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTa                                      <  1:6299991/150‑1 (MQ=255)
                                                                                                                 cTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAg                                     <  2:3686232/150‑1 (MQ=255)
                                                                                                                 cTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCCGCGGCTTGTCGTAg                                     <  1:4714378/150‑1 (MQ=255)
                                                                                                                    gcgcGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTc                                  >  2:2619143/1‑150 (MQ=255)
                                                                                                                       cgTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAAc                               <  1:3001981/150‑1 (MQ=255)
                                                                                                                       cgTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGACGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAAc                               <  2:10294661/150‑1 (MQ=255)
                                                                                                                         tCTTATCAGCATACGCCACATCCGGCATACCATGCCGGCTGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAAccc                             <  1:9640124/150‑1 (MQ=255)
                                                                                                                           ttATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCa                           <  2:5805700/150‑1 (MQ=255)
                                                                                                                            tATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAg                          <  2:6419930/150‑1 (MQ=255)
                                                                                                                             aTCAGCATACGCCACATCCGGCATACCATGCCGGCTGTGGCGTCTCATTACCACGCACTATCCGCCCCGTCTGTTTGAACGGGTTGCGGGTTCAACTGCGGGTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGc                         >  2:3965229/1‑150 (MQ=255)
                                                                                                                               cAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCAc                       <  1:2793333/150‑1 (MQ=255)
                                                                                                                                 gCATACGCCACATCCGGCATAACATGCCGGATGTGGCGTATCATTACAAAGCAATATACGCCACATCTTTTTTAAAGGGTTGCGGTTTCAACAGCGGTTTCGGCGTAAGATCAGCAGCCGGCGGCTAGTAGTAGTTCAAAACCAGCACta                     >  1:9993312/1‑149 (MQ=255)
                                                                                                                                     aCGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCt                 <  1:7694509/150‑1 (MQ=255)
                                                                                                                                     aCGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCt                 <  2:2811000/150‑1 (MQ=255)
                                                                                                                                      cGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTg                <  2:6245926/150‑1 (MQ=255)
                                                                                                                                       gCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTgg               >  2:3640154/1‑150 (MQ=255)
                                                                                                                                        ccACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGgt              <  1:4011541/150‑1 (MQ=255)
                                                                                                                                        ccACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGgt              <  2:554068/150‑1 (MQ=255)
                                                                                                                                          acaTCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGgtgt            >  2:8125689/1‑150 (MQ=255)
                                                                                                                                           caTCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTa           <  1:2850930/150‑1 (MQ=255)
                                                                                                                                            aTCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTAc          >  2:7582860/1‑150 (MQ=255)
                                                                                                                                            aTCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTAc          >  2:6580252/1‑150 (MQ=255)
                                                                                                                                             tCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACt         >  1:7127293/1‑150 (MQ=255)
                                                                                                                                             tCCGGCATACCATGCCGGATGTGGCGTATCCTTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACt         <  2:19818/150‑1 (MQ=255)
                                                                                                                                             tCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACt         >  1:7928419/1‑150 (MQ=255)
                                                                                                                                             tCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACt         >  1:6777078/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTGTACTGCAACTC  >  NC_000913/2304936‑2305233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: