Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,523 G→T 6.2% L72I (CTT→ATT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5230GT 6.2% 1087.5 / 7.4 396L72I (CTT→ATT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (207/143);  new base T (8/33);  total (217/179)
Fisher's exact test for biased strand distribution p-value = 1.44e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 433 total aligned reads displayed.

AGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238374‑238672
                                                                                                                                                     |                                                                                                                                                     
aGTATTTAAATATCCTGTGACAAACGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaag                                                                                                                                                       <  2:198890/150‑1 (MQ=255)
  tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCgtaagta                                                                                                                                                     >  1:4662182/1‑150 (MQ=255)
     tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                                                  >  1:2356163/1‑150 (MQ=255)
      gACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAt                                                                                                                                                 >  1:7460208/1‑150 (MQ=255)
       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTATGAATGATTTTATTATCGTTAGTAAAAtt                                                                                                                                                >  2:4513330/1‑150 (MQ=255)
         atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                              >  1:9118527/1‑150 (MQ=255)
          tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                             >  2:3971064/1‑150 (MQ=255)
          tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                             <  1:2909885/150‑1 (MQ=255)
          tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                             <  2:10208548/150‑1 (MQ=255)
            tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                                           >  2:5593020/1‑150 (MQ=255)
             ccTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGa                                                                                                                                          <  1:4908952/150‑1 (MQ=255)
             ccTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGa                                                                                                                                          <  1:6789637/150‑1 (MQ=255)
              cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                         >  2:1643608/1‑150 (MQ=255)
               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                        >  2:5179548/1‑150 (MQ=255)
               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                        <  1:6506910/150‑1 (MQ=255)
               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                        >  2:6553840/1‑150 (MQ=255)
               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                        >  2:983793/1‑150 (MQ=255)
               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAat                                                                                                                                        >  2:1251702/1‑150 (MQ=255)
                   aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                    <  2:2517853/150‑1 (MQ=255)
                   aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATATTATTATCGTAAGTAAAATTAAATGAATAatt                                                                                                                                    >  1:7546039/1‑150 (MQ=255)
                   aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGATTATTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAAAAttt                                                                                                                                    >  2:8891854/1‑150 (MQ=255)
                    cAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTa                                                                                                                                   <  2:8159692/150‑1 (MQ=255)
                      aaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaa                                                                                                                                 >  1:1805151/1‑150 (MQ=255)
                        aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  2:6518454/150‑1 (MQ=255)
                        aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGCTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  1:1390192/150‑1 (MQ=255)
                          gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAgg                                                                                                                             >  1:805030/1‑150 (MQ=255)
                          gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAgg                                                                                                                             >  1:9429250/1‑150 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAAc                                                                                                                        >  2:1060099/1‑150 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                        >  2:3580529/1‑150 (MQ=255)
                               aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAc                                                                                                                        >  1:7250915/1‑150 (MQ=255)
                                ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACg                                                                                                                       >  1:8882417/1‑150 (MQ=255)
                                    aGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAt                                                                                                                   <  2:186307/150‑1 (MQ=255)
                                      gTGCTTTTACGATTGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGTAATTGACTACCTTCGTTTTTTTGTTTAAGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                 <  2:6198700/150‑1 (MQ=255)
                                      gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGTAAACGAGATGa                                                                                                                 >  1:9485954/1‑150 (MQ=255)
                                       tGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGaa                                                                                                                >  1:4303427/1‑150 (MQ=255)
                                          tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                             >  2:4744801/1‑150 (MQ=255)
                                          tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                             >  2:8481607/1‑150 (MQ=255)
                                          tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                             >  2:9343575/1‑150 (MQ=255)
                                           aTTACGATAGCTATTAGTAAAAATATATGAGTTAGCTGTATTGTTTTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGGAAAACGACATGAAACCg                                                                                                            <  2:2125505/150‑1 (MQ=255)
                                               cGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGc                                                                                                        >  2:9016318/1‑150 (MQ=255)
                                                gATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGca                                                                                                       >  1:2159952/1‑150 (MQ=255)
                                                     cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                  >  2:8326452/1‑150 (MQ=255)
                                                             aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAtt                                                                                          <  2:1153225/150‑1 (MQ=255)
                                                               aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                        <  2:1669557/150‑1 (MQ=255)
                                                                    taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCCAAGCACAGAATCAACAATCTCCAAt                                                                                   >  1:8679884/1‑150 (MQ=255)
                                                                    taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                   >  1:10027544/1‑150 (MQ=255)
                                                                        agTTAGCTGTATTTTTATGTCTGTGGCGAAATTTACTACCTTCGTTTTTTTTATTATCAATGATTTTATTATCTTAAGTAAAATTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATa                                                                               <  1:9750179/150‑1 (MQ=255)
                                                                         gTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGTTTTTATTATAGTAAGTAAAATTACATGGATATTTAAAAAGGAAAACGACATGCAACCGAAGCACAGAATCAACCGTCTCCACTCATaa                                                                              >  1:6246490/1‑150 (MQ=255)
                                                                           tAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaaa                                                                            <  2:8548965/150‑1 (MQ=255)
                                                                            tggtgttttgtttTGTCTGTGGGTAATTTGACTACGTTGGTTTTTTTGATTATGATTGATTTTATTATGTTATTTAAAATTACTTTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAat                                                                           <  1:1016034/147‑1 (MQ=255)
                                                                            aGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTAATATCGTAAGTAAAATTACATGAAAATTTTAAAAGGAAAACGACATGAAAACGAAGCACAGAATAAACAATATACAATCAAAAAat                                                                           >  1:7114436/1‑150 (MQ=255)
                                                                              cTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAatat                                                                         >  2:2886400/1‑150 (MQ=255)
                                                                                 tATTGTTATGTCTGTGGCGAATTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                      <  2:2356163/150‑1 (MQ=255)
                                                                                  tttGTTTTGTCTGTGGCGAAATTTACTACCTTCTTTTTTTTGATTAATAATTTTTTTATTATCGTAAGTAAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTcc                                                                     <  1:5508313/149‑1 (MQ=255)
                                                                                  tttGTTTTGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTTATTAAGATTTATTTTTTTATCGTAAGTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTcc                                                                     <  1:3123814/149‑1 (MQ=255)
                                                                                     gTTTTGTCTGTGGCTAATTGGACTACCTTCGTTTTTTTTATTAAGAATTTTTTTATTATCTTATTTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                  <  1:3519395/150‑1 (MQ=255)
                                                                                       tATGTGTGTGGCGAAATTTAATACTTTCGTTTTTTTGATTAAGAATGATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                <  1:8172009/150‑1 (MQ=255)
                                                                                       tATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                >  2:9584111/1‑150 (MQ=255)
                                                                                       tATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTAATATCGTAAGTAAAATTACAAGAAAATTTAAAAAGGAAAACCACAAGAAACCCAAGCACACAAACAACATTCACCAAACATAAAAAAATTACGTGGa                                                                >  1:5924852/1‑150 (MQ=255)
                                                                                        ttGTCTGTGGCTAAATTGACTACCTTGGTTTTTTTGATTAAGAATTATTTTATTATCTTAAGTAAAATTACTTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                                               <  1:8742608/149‑1 (MQ=255)
                                                                                        aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                                               <  2:7250915/150‑1 (MQ=255)
                                                                                         tGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTTATAATTATTTTATTATCGTAAGTAAAATTAAAATAATATTTAAAAATGAAAAAGACAAGAAACCCAAGCACACAAAAAAAAATCTCCAAAAAAAAAAAATTTGCGGGGTGc                                                              >  1:1050704/1‑150 (MQ=255)
                                                                                            cTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                           <  2:548222/150‑1 (MQ=255)
                                                                                                 gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                      <  2:4218245/150‑1 (MQ=255)
                                                                                                 gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGACTATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCAAAAAAAATTACCGTGGAGCAGTatata                                                      >  2:5359421/1‑149 (MQ=255)
                                                                                                    aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                   <  2:7044347/150‑1 (MQ=255)
                                                                                                      aTTGACTACCTTCGTTTTTTTGATTAAGATTTATTTTATTATGGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGa                                                 <  1:4139549/150‑1 (MQ=255)
                                                                                                       ttGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCTTAAGTAAATTTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                <  2:8698421/150‑1 (MQ=255)
                                                                                                       ttGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                <  2:304767/150‑1 (MQ=255)
                                                                                                       ttGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                >  2:5021181/1‑150 (MQ=255)
                                                                                                       ttGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                <  2:768174/150‑1 (MQ=255)
                                                                                                       attaCTACCTACTTTTTTATTATTAATAATGATTTTATTAACTAAATAAAAATTACATTAAAATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                <  2:9485954/147‑1 (MQ=255)
                                                                                                         gACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAata                                              >  2:599440/1‑150 (MQ=255)
                                                                                                           cTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAAtata                                            >  2:3132577/1‑150 (MQ=255)
                                                                                                            tACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATag                                           <  1:1594651/150‑1 (MQ=255)
                                                                                                              ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  2:6835787/150‑1 (MQ=255)
                                                                                                                ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                       >  2:7895698/1‑150 (MQ=255)
                                                                                                                ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                       <  2:10052996/150‑1 (MQ=255)
                                                                                                                  cGTTTTTTTTATTAAGAATGATTTTATTATAGTAATTAAAATTACATGAATATTTAAAAATGAAAAAGACATGAAAACGAAGCACAGAATAAACATTATACAATCATAAAATATTTAAGTGGAGCATTTTATTATTTAATATAGAGGttt                                     >  1:5312524/1‑150 (MQ=255)
                                                                                                                     ttttttTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                  <  2:7565907/150‑1 (MQ=255)
                                                                                                                          ttaTTAAGATTTTTTTTATTATCTTAATTAAAATTAAATTAATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCgg                             <  2:4388681/148‑1 (MQ=255)
                                                                                                                           taTTAAGAATGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGt                            <  1:3871696/149‑1 (MQ=255)
                                                                                                                           gATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGt                            >  2:1437431/1‑150 (MQ=255)
                                                                                                                            agtAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATCTTTCCGTGGAGCATTGTATTAGTGAATATAGAGGTTTAACTCCGGTa                           >  1:2620230/3‑150 (MQ=255)
                                                                                                                            aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                           >  1:9958616/1‑150 (MQ=255)
                                                                                                                            aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                           >  1:96212/1‑150 (MQ=255)
                                                                                                                              tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                         >  2:6326184/1‑150 (MQ=255)
                                                                                                                                aGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAACAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaa                       >  1:6157525/1‑150 (MQ=255)
                                                                                                                                 gAATTATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                      <  1:5593020/150‑1 (MQ=255)
                                                                                                                                  aaTGATTTTATTATCGAAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                     >  1:7853932/1‑150 (MQ=255)
                                                                                                                                            ttatCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt           <  1:5801856/150‑1 (MQ=255)
                                                                                                                                             tatCGTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          <  1:1897926/150‑1 (MQ=255)
                                                                                                                                             tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          >  2:2964541/1‑150 (MQ=255)
                                                                                                                                              atCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTg         <  1:7628985/150‑1 (MQ=255)
                                                                                                                                               tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGa        >  1:4467133/1‑150 (MQ=255)
                                                                                                                                               tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGa        >  2:6418178/1‑150 (MQ=255)
                                                                                                                                                 gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt      >  2:9255184/1‑150 (MQ=255)
                                                                                                                                                  taagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATg     >  2:5916915/1‑150 (MQ=255)
                                                                                                                                                    agtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa   <  2:2926788/150‑1 (MQ=255)
                                                                                                                                                     gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg  <  1:3285072/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238374‑238672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: