BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,411,234	C→A	5.7%	S40I (AGC→ATC)	yfbS ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,411,234	0	C	A	5.7%	1747.8 / 4.2	556	S40I (AGC→ATC)	yfbS	putative transporter
Reads supporting (aligned to +/- strand): ref base C (303/196); new base A (2/50); total (305/251)
Fisher's exact test for biased strand distribution p-value = 7.29e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 563 total aligned reads displayed.

TATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT  >  NC_000913/2411085‑2411383

tATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑c                                                                                                                                                       >  2:3402237/1‑150 (MQ=255)

 aTTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑Ct                                                                                                                                                      <  2:2749158/150‑1 (MQ=255)

    gCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGTCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTaa                                                                                                                                                   <  2:4158827/150‑1 (MQ=255)

    gCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTaa                                                                                                                                                   <  1:1096699/150‑1 (MQ=255)

    gCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTaa                                                                                                                                                   <  2:2882593/150‑1 (MQ=255)

     cccGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAAt                                                                                                                                                  <  2:3612233/150‑1 (MQ=255)

      ccGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATg                                                                                                                                                 >  1:2110466/1‑150 (MQ=255)

      ccGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATg                                                                                                                                                 <  1:2533443/150‑1 (MQ=255)

       cGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGc                                                                                                                                                >  1:9499180/1‑150 (MQ=255)

        gCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCa                                                                                                                                               <  2:507763/150‑1 (MQ=255)

        gCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCa                                                                                                                                               >  1:6603581/1‑150 (MQ=255)

         cAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCaa                                                                                                                                              >  2:1184954/1‑150 (MQ=255)

         cAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCaa                                                                                                                                              >  2:8028716/1‑150 (MQ=255)

           aCTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAAc                                                                                                                                            <  1:5612466/150‑1 (MQ=255)

             tttGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAAcgc                                                                                                                                          <  2:8301996/150‑1 (MQ=255)

                  ccAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACac                                                                                                                                     >  1:4722801/1‑149 (MQ=255)

                  ccAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACaa                                                                                                                                     >  1:7008007/1‑150 (MQ=255)

                   cAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAAt                                                                                                                                    >  1:10277377/1‑150 (MQ=255)

                    aGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATa                                                                                                                                   >  1:3637388/1‑150 (MQ=255)

                    aGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATa                                                                                                                                   <  2:4495141/150‑1 (MQ=255)

                     gCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATaa                                                                                                                                  <  2:10109248/150‑1 (MQ=255)

                     gCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATaa                                                                                                                                  <  1:6267359/150‑1 (MQ=255)

                      ccATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATaac                                                                                                                                 <  1:5225715/150‑1 (MQ=255)

                      ccATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATaac                                                                                                                                 <  2:7637881/150‑1 (MQ=255)

                       cATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATaaca                                                                                                                                <  1:2766817/150‑1 (MQ=255)

                         tGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATaacaaa                                                                                                                              <  2:5506815/150‑1 (MQ=255)

                          gTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATaacaaac                                                                                                                             <  1:8888104/150‑1 (MQ=255)

                            tCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAacaaacaa                                                                                                                           >  1:8510692/1‑150 (MQ=255)

                             cccATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAAcaaacaaa                                                                                                                          >  2:1066286/1‑150 (MQ=255)

                               cATTACGGTGGCAACACCGGTACTGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGc                                                                                                                        >  2:9560824/1‑150 (MQ=255)

                                 ttACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGa                                                                                                                      >  2:7750674/1‑150 (MQ=255)

                                         gCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATaacaa                                                                                                                               >  1:5881928/1‑133 (MQ=255)

                                           aaCACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCAt                                                                                                            >  2:1840834/1‑150 (MQ=255)

                                            acacCGTTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAAGTTTTGGATCAGAAATGCCGTAAAATAATTCTGGTAATGTCAGCGTTCCT‑ATTAATGCAAACGCGACAATAAAAAACAAAGCGACCGCATCCATa                                                                                                           <  2:3291868/150‑1 (MQ=255)

                                              acCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACg                                                                                                         >  2:1534160/1‑150 (MQ=255)

                                              acCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACg                                                                                                         >  1:1883257/1‑150 (MQ=255)

                                                 ggTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCAc                                                                                                      >  2:5980071/1‑150 (MQ=255)

                                                  gTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCAct                                                                                                     >  2:469214/1‑150 (MQ=255)

                                                     cGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCCAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTg                                                                                                  >  1:767522/1‑150 (MQ=255)

                                                      ggACCAAACAATCGCAAATAATAAAAAGGGCGGAAATCAGTAAAAGTTTTGTATCATAAAAGCCGTAAAATACTTCTGGTAGTTTAAGGTTTCCG‑CTTAATGCAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGc                                                                                                 <  2:8682358/150‑1 (MQ=255)

                                                        aCCAAACCATCGCCAATAATAAACAAGGCGGCACTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCCTACGCACTCTGccc                                                                                               >  1:7174671/1‑150 (MQ=255)

                                                          cacaCCATCGCCTATAATAACCCCGGCGGCAATCTGGACACCGTTTTGATAATAAATGCGGTAAAATACTTCTGGGACTGTCCGGGTTCCG‑CTTTATGCAACCGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGt                                                                                             <  2:6101618/147‑1 (MQ=255)

                                                           aaaCCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGGTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTc                                                                                            >  2:9652990/1‑150 (MQ=255)

                                                                 tCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAAc                                                                                      >  2:2154294/1‑150 (MQ=255)

                                                                   gCCAATAATAAACAAGGCGGAAATCAGGACAACGTTAGGATCAGAAAAGCCGTAAAATACTTCTGGGAGTGTCTGCGTTCCG‑CTTAATGCAAACGCTACAATAACAAAAAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACaa                                                                                    <  2:9857992/150‑1 (MQ=255)

                                                                    ccAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAg                                                                                   >  2:5577920/1‑150 (MQ=255)

                                                                      aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGAc                                                                                 >  1:9265159/1‑150 (MQ=255)

                                                                       ataataAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACg                                                                                >  1:6029463/1‑150 (MQ=255)

                                                                          ataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATTCATACGCACTCTGCCCGTCGCAAACAAGACGATg                                                                             <  2:6591798/150‑1 (MQ=255)

                                                                          ataaAAATGTCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATAATTCTGGGACTTTCAGCTTTCCG‑CTTAATTCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATg                                                                             <  2:6507854/150‑1 (MQ=255)

                                                                               cacggcggaAATCGGGACAAGGTTTGTATCATAAACGCGGTAAATTACTTGGGGTAGTGTCAGGGTTCCG‑CTTAATGCAACCGCGAAAATACCAACCAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAAc                                                                        <  2:3036763/147‑1 (MQ=255)

                                                                               cAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAAc                                                                        >  2:7085989/1‑150 (MQ=255)

                                                                                 cggcggaAATCATGACAACGTTTGTATAATAAAAGCGGTAAATTACTTCTGGTACTGTCTGCTTTCCT‑CTTAATGAAAAGGCTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACgg                                                                      <  2:2614738/149‑1 (MQ=255)

                                                                                     ggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAATGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAg                                                                  >  2:6358334/1‑150 (MQ=255)

                                                                                        aaTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTaa                                                               >  2:4420126/1‑150 (MQ=255)

                                                                                        aaTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTaa                                                               >  2:9333333/1‑150 (MQ=255)

                                                                                        aaTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTaa                                                               >  2:7326094/1‑150 (MQ=255)

                                                                                         aTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt                                                              <  2:6747268/150‑1 (MQ=255)

                                                                                           cAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGa                                                            <  2:9183087/150‑1 (MQ=255)

                                                                                            aGGACAACGTTACGATCAGAAAAGACGGAAAATACTTCTGGGACTTTCAGCGTTAAT‑ATTAATTAAAAATATACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGaa                                                           >  2:8972733/1‑150 (MQ=255)

                                                                                              gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                         >  1:2842129/1‑150 (MQ=255)

                                                                                                aaaCTTTAGGATCATAAAAGCGGTAAAATACTTCTGGGACTGTCAGCGTTCCG‑ATTACTGCAAACGCGAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGaa                                                       <  2:9073178/149‑1 (MQ=255)

                                                                                                     ttAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaa                                                  >  1:4991363/1‑150 (MQ=255)

                                                                                                       tttaTCATAAACGCCTTAAAATACTTGTGGTAGTGTCCG‑GTTACGTATTATTGAAACCGATACAATAAAAAACACCGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                <  1:1184954/147‑1 (MQ=255)

                                                                                                       tgtaTCTGAAATGCGGTAAATTAGTTGGGGGAGTGTCTGGGTTCGG‑CTTATTGAAACGGCGAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                <  1:10007013/147‑1 (MQ=255)

                                                                                                         gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                              >  2:3970446/1‑150 (MQ=255)

                                                                                                          ataataaaaGCGGTAAAATACTTGTGGTAGTTTCAGCGTTCCG‑ATTATTGAAAACGCTAAAATAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                             <  1:7283700/145‑1 (MQ=255)

                                                                                                          aTCATAAAAGCGTTAAATTACTTCTGGGACTTTCAGGTTTCCG‑ATTATGGAAACCGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                             <  1:4241789/150‑1 (MQ=255)

                                                                                                          aTCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                             >  1:7732724/1‑150 (MQ=255)

                                                                                                           tCATAAAAGCCGGAAAATACTTCTGGGAGTGTCAGCGTTCCG‑ATTAATGCAAACGCGACAATAACAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAt                                            <  2:6924201/150‑1 (MQ=255)

                                                                                                             aGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                          >  1:5548953/1‑150 (MQ=255)

                                                                                                               aaaaGCCGTAAAATACTTCTGGGACTTTCTGCGTTCGG‑ATTAATGCAAAGGCGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc                                        <  2:7892504/150‑1 (MQ=255)

                                                                                                                 aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                      >  2:3875583/1‑150 (MQ=255)

                                                                                                                    ccGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                   <  2:10204163/150‑1 (MQ=255)

                                                                                                                       gAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                >  1:155109/1‑150 (MQ=255)

                                                                                                                       gAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                >  2:247212/1‑150 (MQ=255)

                                                                                                                         aaaTACTCCGGGGACTGTCCGCTTCCCG‑ATTAATGAAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAAc                              <  2:323687/150‑1 (MQ=255)

                                                                                                                          aaTACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                             >  1:9416965/1‑150 (MQ=255)

                                                                                                                            tACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTa                           >  1:8011395/1‑150 (MQ=255)

                                                                                                                             aCTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCACCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTAt                          >  1:3328807/1‑150 (MQ=255)

                                                                                                                               ttCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATcc                        >  1:4856529/1‑150 (MQ=255)

                                                                                                                                  tggttcTGTCAGCGTTCCG‑CTTAATGCAAACGCTAAAATAACAAACCAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                     <  1:8342616/145‑1 (MQ=255)

                                                                                                                                  tGGGAGTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                     <  2:5274737/150‑1 (MQ=255)

                                                                                                                                  tGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                     <  1:6801985/150‑1 (MQ=255)

                                                                                                                                   gggACTGTCCGGTTTCCG‑ATTAATGCAACCGCGACAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                    <  2:1762298/150‑1 (MQ=255)

                                                                                                                                   gggACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                    <  2:4291230/150‑1 (MQ=255)

                                                                                                                                   gggACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                    <  2:5851793/150‑1 (MQ=255)

                                                                                                                                   gggACGGCCCGCGTTCCG‑CTTAATGCAACCGCCAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                    <  1:2646759/150‑1 (MQ=255)

                                                                                                                                    ggACTGTCAGCGTCCCG‑CTAAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGtt                   <  2:2007490/150‑1 (MQ=255)

                                                                                                                                        tGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTGGTTAAtt               >  1:8664682/1‑150 (MQ=255)

                                                                                                                                           cAGCGTTCCG‑CTTAATGCAAACGCTACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAg            <  2:569301/150‑1 (MQ=255)

                                                                                                                                            aGCTTTCCG‑ATTAATGAAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgg           <  2:4654267/150‑1 (MQ=255)

                                                                                                                                            aGCGTTCCG‑CTTAATGCAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgg           <  1:3165761/150‑1 (MQ=255)

                                                                                                                                             gCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggg          >  2:5197511/1‑150 (MQ=255)

                                                                                                                                              gtttCCG‑ATTAATGAAACCGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAACTCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgggg         <  2:2333718/148‑1 (MQ=255)

                                                                                                                                               gTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg        >  1:242436/1‑150 (MQ=255)

                                                                                                                                                ttCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGa       >  1:1699014/1‑150 (MQ=255)

                                                                                                                                                 tCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGAt      >  1:5806890/1‑150 (MQ=255)

                                                                                                                                                    g‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAc   >  2:490397/1‑150 (MQ=255)

                                                                                                                                                    g‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAc   >  1:9877794/1‑150 (MQ=255)

TATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACT  >  NC_000913/2411085‑2411383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`