Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,411,241 C→A 7.5% A38S (GCA→TCA)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,411,2410CA 7.5% 1696.2 / 15.7 542A38S (GCA→TCA) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (297/184);  new base A (3/57);  total (300/242)
Fisher's exact test for biased strand distribution p-value = 1.27e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 553 total aligned reads displayed.

CGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCT  >  NC_000913/2411092‑2411390
                                                                                                                                                     |                                                                                                                                                     
cGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGc                                                                                                                                                       <  1:2315554/150‑1 (MQ=255)
  cAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCaa                                                                                                                                                     >  2:8028716/1‑150 (MQ=255)
    aCTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTATCAGCGTTCCGCTTAATGCacac                                                                                                                                                   >  1:4948059/1‑150 (MQ=255)
      tttGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAAcgc                                                                                                                                                 >  1:6607987/1‑150 (MQ=255)
      tttGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAAcgc                                                                                                                                                 >  1:172579/1‑150 (MQ=255)
      tttGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAAcgc                                                                                                                                                 <  2:8302305/150‑1 (MQ=255)
        tGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATACACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGa                                                                                                                                               <  1:1324799/150‑1 (MQ=255)
            cAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAAt                                                                                                                                           >  1:10277377/1‑150 (MQ=255)
             aGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATa                                                                                                                                          <  2:4493067/150‑1 (MQ=255)
             aGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATa                                                                                                                                          >  1:3630725/1‑150 (MQ=255)
             aGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATa                                                                                                                                          <  2:5178394/150‑1 (MQ=255)
              gCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaa                                                                                                                                         <  2:10109248/150‑1 (MQ=255)
               ccATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaac                                                                                                                                        <  1:5225715/150‑1 (MQ=255)
                cATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaaca                                                                                                                                       <  1:2766817/150‑1 (MQ=255)
                cATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaaca                                                                                                                                       <  2:5022620/150‑1 (MQ=255)
                cATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaaca                                                                                                                                       <  2:3548820/150‑1 (MQ=255)
                 aTGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaacaa                                                                                                                                      <  2:6527482/150‑1 (MQ=255)
                     tCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAacaaacaa                                                                                                                                  >  1:8510692/1‑150 (MQ=255)
                      cccATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAAcaaacaaa                                                                                                                                 <  1:5619580/150‑1 (MQ=255)
                        cATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGc                                                                                                                               >  1:7228168/1‑150 (MQ=255)
                        cATTACGGTGGCAACACCGGTACGGACCAAACAATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAAGAACAAACAAAGc                                                                                                                               >  2:9560787/1‑150 (MQ=255)
                          ttACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGa                                                                                                                             >  2:7750674/1‑150 (MQ=255)
                           tACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGAc                                                                                                                            >  2:9189457/1‑150 (MQ=255)
                                tGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCAt                                                                                                                       >  1:9283566/1‑150 (MQ=255)
                                 ggCAACACCGGTGCGGACCAAACCATCTCCAATAATAAACAAGGCGGCAATCTGTACAACGTTAGGATCAGAAATGCCGGAAAATACTTCTGGGACTGTCAGCGTTCGGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATc                                                                                                                      <  2:2388490/150‑1 (MQ=255)
                                  gCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATaacaa                                                                                                                                      <  2:5881928/133‑1 (MQ=255)
                                    aaCACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCAt                                                                                                                   >  1:5940664/1‑150 (MQ=255)
                                     acacCGGTACGGACCAACCCATCGCCAATAATAAACATGGCGGCAATCTGGACAACTTTCGGTTCTTAAAAGCGGGAAATTACTTCTGGGACTGTCTGGGTTCCGCTTAATGAAAACGCGACAATAACAAACAACGCGACCGCATCCATa                                                                                                                  <  2:2298130/150‑1 (MQ=255)
                                           gTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCAct                                                                                                            >  2:2545828/1‑150 (MQ=255)
                                           gTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCAct                                                                                                            >  2:469214/1‑150 (MQ=255)
                                            tACGGACCAAACCATCGCCAATAATAACCAAGGCGGCAATCAGGACAAGGTTTGTATCATAAAAGCCGTAAAATACTTCTGGTACTGTCTGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCActc                                                                                                           <  2:3303624/150‑1 (MQ=255)
                                              cGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCCAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTg                                                                                                         >  1:767522/1‑150 (MQ=255)
                                                 aCCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGccc                                                                                                      >  2:200666/1‑150 (MQ=255)
                                                 aCCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGAAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGccc                                                                                                      <  2:3260211/150‑1 (MQ=255)
                                                 aCCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCGGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGccc                                                                                                      >  2:2073393/1‑150 (MQ=255)
                                                   cAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCga                                                                                                    >  1:6115656/1‑149 (MQ=255)
                                                    aaaCCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGGTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTc                                                                                                   >  2:9652990/1‑150 (MQ=255)
                                                     aaCCATCGCCAATAATAACCAAGGCGGCAATCAGGACAACTTTAGGATCAGAAAAGCCTGAAAATACTTCTGGGACTGTCAGCGTTCCGATTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCg                                                                                                  <  2:7130420/150‑1 (MQ=255)
                                                          tCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGTGACCGCATCCATACGCACTCTGCCCGTCGCacac                                                                                             >  2:5469364/1‑150 (MQ=255)
                                                          tCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAAc                                                                                             >  2:6004122/1‑150 (MQ=255)
                                                            gCCAATAATAAACAAGGCGGAAATCAGGACAACGTTAGGATCAGAAAAGCCGTAAAATACTTCTGGGAGTGTCTGCGTTCCGCTTAATGCAAACGCTACAATAACAAAAAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACaa                                                                                           <  2:9857992/150‑1 (MQ=255)
                                                               aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGAc                                                                                        >  2:2652432/1‑150 (MQ=255)
                                                                   ataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATg                                                                                    >  2:3952997/1‑150 (MQ=255)
                                                                   ataaAAAAGGCGCCAATCATGACAACGTTAGCAACAGAAAAGCCGTAAAATACTTCTGGGACTGTCAGCTTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATg                                                                                    <  2:1647965/150‑1 (MQ=255)
                                                                    taaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATgg                                                                                   >  2:7662743/1‑150 (MQ=255)
                                                                      aaCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCa                                                                                 >  2:6409498/1‑150 (MQ=255)
                                                                       aCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCac                                                                                >  2:4375656/1‑149 (MQ=255)
                                                                          cggcggcAATAAGGACAACGTTAGGATCAGAAAAGCCGTAAAATAGTTCTGGGACTGTCTGCGTTCCGCTTAATGCAAACGCTACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACgg                                                                             <  2:3033489/149‑1 (MQ=255)
                                                                            gcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGcc                                                                           >  2:3185816/1‑150 (MQ=255)
                                                                             cggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCa                                                                          >  1:5527945/1‑150 (MQ=255)
                                                                             cggaAATCTGGACAACTTTTGGATCAGAAAAGCCGGAAAATAGTTCTGGGACTGTCTGCGTTCCGCTTAATGCAACCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCa                                                                          <  2:1081487/150‑1 (MQ=255)
                                                                              ggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAg                                                                         >  1:4786964/1‑150 (MQ=255)
                                                                              ggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAg                                                                         >  1:9896100/1‑150 (MQ=255)
                                                                                 aaTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTaa                                                                      >  2:1854136/1‑150 (MQ=255)
                                                                                 aaTCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTaa                                                                      >  2:7326094/1‑150 (MQ=255)
                                                                                       gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                >  2:4153769/1‑150 (MQ=255)
                                                                                         aaaCTTTAGGATCATAAAAGCGGTAAAATACTTCTGGGACTGTCAGCGTTCCGATTACTGCAAACGCGAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGaa                                                              <  2:9073178/149‑1 (MQ=255)
                                                                                           agttttggATAATAAAAGCCTTAAAATACTTCGGTGATTGACCGCGTCCCGATTAATCAAACCGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAcc                                                            <  1:8902609/144‑1 (MQ=255)
                                                                                              ttAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaa                                                         >  1:9676237/1‑150 (MQ=255)
                                                                                                tgtaTCTGAAATGCGGTAAATTAGTTGGGGGAGTGTCTGGGTTCGGCTTATTGAAACGGCGAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                       <  1:10007013/147‑1 (MQ=255)
                                                                                                aGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                       >  1:2840373/1‑150 (MQ=255)
                                                                                                  gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                     >  2:9001660/1‑150 (MQ=255)
                                                                                                   aTCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                    >  1:7732724/1‑150 (MQ=255)
                                                                                                    tCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAt                                                   >  1:6466083/1‑150 (MQ=255)
                                                                                                        aaaaGCCGTAAAATACTTCTGGGACTTTCTGCGTTCGGATTAATGCAAAGGCGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAc                                               <  2:7892504/150‑1 (MQ=255)
                                                                                                          aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                             >  2:2813912/1‑150 (MQ=255)
                                                                                                           aGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGATTAATGCAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGt                                            <  1:6900634/150‑1 (MQ=255)
                                                                                                             ccGTAAAATACTTCTGGTACTGTCAGGGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                          <  2:6997928/150‑1 (MQ=255)
                                                                                                             ccGCAAAATACGACTGCGACGGACAGCGGTCCGCTACAGGCAACCGCTACAATAACAACCAAAGCGCCCGCATCCATACGCACTCTGCCCGTCGCAAACACGCCGATGGCATCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                          <  2:10204164/150‑1 (MQ=255)
                                                                                                                gAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                       >  2:8697462/1‑150 (MQ=255)
                                                                                                                 aaaaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                      <  2:6815277/150‑1 (MQ=255)
                                                                                                                   aaTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                    >  1:9417446/1‑150 (MQ=255)
                                                                                                                     tACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTa                                  >  1:8011395/1‑150 (MQ=255)
                                                                                                                          cTGGGACTGTCAGCTTTCGGATTAATGAAAACGCGAAATTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCtt                             <  2:1225713/150‑1 (MQ=255)
                                                                                                                          cTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCtt                             <  2:4278704/150‑1 (MQ=255)
                                                                                                                           tggttcTGTCAGCGTTCCGCTTAATGCAAACGCTAAAATAACAAACCAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                            <  1:8342616/145‑1 (MQ=255)
                                                                                                                           tGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTg                            >  2:5649055/1‑150 (MQ=255)
                                                                                                                            gggACTGTCCGGTTTCCGATTAATGCAACCGCGACAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                           <  2:1762298/150‑1 (MQ=255)
                                                                                                                            gggACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                           <  1:3126916/150‑1 (MQ=255)
                                                                                                                            gggACGTTCAGCGTTCCGCTTAATGCAAACGCCACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                           <  1:1155683/150‑1 (MQ=255)
                                                                                                                            gggACGGTCCGCTTCCCGCTTAATGCAACCGCGACAAAAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                           <  1:2646577/150‑1 (MQ=255)
                                                                                                                                cTGACAGCGTCCCGCTTAATGAAAACGCTACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAt                       <  1:2027030/150‑1 (MQ=255)
                                                                                                                                 tGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAtt                      >  2:6606503/1‑150 (MQ=255)
                                                                                                                                 tGTCAGCGTTCCGATTAATGAAACCGCGAAAAAAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAtt                      <  1:8680158/150‑1 (MQ=255)
                                                                                                                                     aGCTTTCCGATTAATGAAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgg                  <  2:4654267/150‑1 (MQ=255)
                                                                                                                                     aGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACTACTTATCCTTGTTAATTGAgg                  >  2:5799301/1‑150 (MQ=255)
                                                                                                                                     aGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgg                  >  1:2522090/1‑150 (MQ=255)
                                                                                                                                     aGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgg                  >  1:161087/1‑150 (MQ=255)
                                                                                                                                      gCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggg                 >  1:7046306/1‑150 (MQ=255)
                                                                                                                                      gCGTTCCGCTTAATGAAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggg                 >  2:3706002/1‑150 (MQ=255)
                                                                                                                                         ttCCGTTTATTGTAAACGCTAAATTAACAAACCTCGCGAGCGCATCCATACGCACTCTGCCCGTCGCTAACACGACGATGGCTACGTCCAGTTATGATAGAACCGAAATCAATTCACCGTTCACAACTTTTCCTTGTTAATTGAGGGGGa              <  2:6202310/150‑1 (MQ=255)
                                                                                                                                         ttCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTGGTTAATTGAGGGGGa              >  2:5254801/1‑150 (MQ=255)
                                                                                                                                            cGCTTAATGCAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGa           <  1:3297325/150‑1 (MQ=255)
                                                                                                                                              cTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt         <  2:5558856/150‑1 (MQ=255)
                                                                                                                                              cTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt         >  1:5814604/1‑150 (MQ=255)
                                                                                                                                               ttAATGCAAACGCGAAAAAAACAACCAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACtt        <  1:4000254/150‑1 (MQ=255)
                                                                                                                                                  aTGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCACCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTGATCCTGGTTAATTGAGGGGGATGACTTGag     >  1:7513933/1‑149 (MQ=255)
                                                                                                                                                    gCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTc   >  1:454028/1‑150 (MQ=255)
                                                                                                                                                     cAAACGCGACAATCACAAACCAAGTGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAGCAATTCACCGTTCACAACTTATCCTGGTTAATAGAGGGGGATGACGGGATTCt  >  2:481265/1‑150 (MQ=255)
                                                                                                                                                     cAAACGCGACAATAACAAACAAAGTGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCt  >  2:9268404/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCT  >  NC_000913/2411092‑2411390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑