Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,460,604 A→C 11.5% intergenic (‑135/+46) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,6040AC11.5% 1183.2 / 41.1 453intergenic (‑135/+46)fadI/yfcZbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base A (228/161);  new base C (2/62);  total (230/223)
Fisher's exact test for biased strand distribution p-value = 4.73e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 509 total aligned reads displayed.

TAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGT  >  NC_000913/2460455‑2460751
                                                                                                                                                     |                                                                                                                                                   
tAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGagaaaagaaa                                                                                                                                                     <  2:5922162/150‑1 (MQ=255)
tAAAACCTGACCCATAACACTCCCCTGTAAAAAATAAATACAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAACGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAgacaagaaaa                                                                                                                                                    <  2:9389975/150‑1 (MQ=255)
     ccTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGcc                                                                                                                                                >  2:4963069/1‑150 (MQ=255)
           ccATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGcc                                                                                                                                          >  2:1090877/1‑150 (MQ=255)
            cATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCa                                                                                                                                         >  2:2711284/1‑150 (MQ=255)
             aTAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAg                                                                                                                                        >  2:9005927/1‑150 (MQ=255)
             aTAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACAATTTTTTTACATTAAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCACCTGCCAg                                                                                                                                        >  2:7649318/1‑150 (MQ=255)
              tAACACTCCCCTGTAAAAAAATAAATAATGTGTTCTGACATTATAATTTTCTTAACAATTTTTTTAATTTTAGCCATGTGTAGAAATGGTAAAGTGGTAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGc                                                                                                                                       <  2:9761778/150‑1 (MQ=255)
                    cccccTGTAAAAAAATAAATAACGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTCGCCACGTGGAGAAAAGGGAAAGTGGGCGCTATGACACAGAGATAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGa                                                                                                                                 <  2:2921447/149‑1 (MQ=255)
                      cccTGTAAAAAAATAAATACCGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACa                                                                                                                               <  2:8591447/150‑1 (MQ=255)
                      cccTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACa                                                                                                                               >  1:9198174/1‑150 (MQ=255)
                       ccTGTAAAAAAATAAATAACGTGGACTGACCTGAACATAGTCTTAACAATTTTTTTACATTTCGCCAAGTGGAGAAAAGGTAATGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAg                                                                                                                              <  2:6109289/150‑1 (MQ=255)
                       ccTGAAAAAAAATAAATACCGTGGTCTGACCTGATCTTAGTCTTAACCTTTTTTTTAATTTTAGCCAAGGGGATAAAAGGGAATGTGGGAGCTATTACACAGAGACAAAAGACGAACATAAACGAAAACGCCCCTGCCAGCAACTGACAg                                                                                                                              <  2:918306/150‑1 (MQ=255)
                         tttAAAAAAATAATTAAATTGTTCTTACCTGATATTATTCTTATCCATTTTTTTACATTTAGCCACGTGTTTAATCGGGATTGTGTTAGCTATTACACAGCTATAAAATAAGAACAGAAAACAAAACGCCCCTGCCAGCAACTGACAggg                                                                                                                            <  2:7987196/148‑1 (MQ=255)
                         tGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAggg                                                                                                                            >  1:8458014/1‑150 (MQ=255)
                          gTAAAAAAATAACTAATGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTGAGCCACGTGGAGAAACGGGAAAGTGGGAGCTATGACACCGAGAGAAACGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgggg                                                                                                                           <  2:9341665/150‑1 (MQ=255)
                           tAAAAAAATAAATAAAGTGTTCTGACCTTATAATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGc                                                                                                                          <  2:5706669/150‑1 (MQ=255)
                              aaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgta                                                                                                                       >  1:1901236/1‑150 (MQ=255)
                                aaataaatAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgtacg                                                                                                                     >  1:7751025/1‑150 (MQ=255)
                                aaataaatAAAGTGGTCTGACCAGATCATAGTCTTAACCATTTATTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCAGCCAGCAACTGACAGGGGcgtacg                                                                                                                     >  2:6601458/1‑150 (MQ=255)
                                  ataaataaAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgtacgta                                                                                                                   >  2:8379818/1‑150 (MQ=255)
                                  ataaataaAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgtacgta                                                                                                                   >  2:3494284/1‑150 (MQ=255)
                                    aaataaaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAcc                                                                                                                 >  2:7864085/1‑150 (MQ=255)
                                       gcccgTGGTCTGACCGGTCCATAGTCTTAACCATTTTTTTACATTTAGCCACGTGGCGAAACGGGAATGTGGGAGCTATGACACAGAGAGAAAAGATGCCGAGAACAGAAACCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCga                                                                                                              <  2:7438330/146‑1 (MQ=255)
                                         aaGTGGTCTTACCTGATCATTGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGATAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCgaga                                                                                                            <  2:10220495/150‑1 (MQ=255)
                                             ggactgacctgacCTTTGTCTTAACCATTTTTTTACATTTCGCCAAGTGGAGATAAGGGAATGTGGGAGCTATGACACAGAGCGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGaa                                                                                                        <  2:504785/147‑1 (MQ=255)
                                              gTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAt                                                                                                       >  2:3697744/1‑150 (MQ=255)
                                               tCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAAtt                                                                                                      >  2:2474197/1‑150 (MQ=255)
                                                    ccTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGc                                                                                                 >  1:5436964/1‑150 (MQ=255)
                                                     cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGACTTAACGCa                                                                                                >  2:6428340/1‑150 (MQ=255)
                                                     cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                                                                >  1:2533437/1‑150 (MQ=255)
                                                       gATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGACTTAACGCAgt                                                                                              >  1:2856446/1‑149 (MQ=255)
                                                        aTCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGACGAAGAGAAAAGACAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAc                                                                                             <  2:6870310/150‑1 (MQ=255)
                                                        aTCATAGTATTAACATTTTTTTTAATTTTCGCCATGTGGATAAATGGGAATGTGGTAGCAATGACACCGAGACAAAAGAACAAGAGAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAc                                                                                             <  1:4187494/150‑1 (MQ=255)
                                                          cATTGTTTTAACATTTTTTTTAATTTTCGCCATGTGTATAAATGGGAATGTGGGTGCTATGACACAGAGATAAAATAAGACGACAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAccc                                                                                           <  1:1648971/150‑1 (MQ=255)
                                                           aTAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAATCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCa                                                                                          >  2:8748514/1‑150 (MQ=255)
                                                            tAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAg                                                                                         >  2:3608244/1‑150 (MQ=255)
                                                              gTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                                                                       >  1:6180123/1‑150 (MQ=255)
                                                              gTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                                                                       >  1:5997686/1‑150 (MQ=255)
                                                                  tAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGACTTAACGCAGACCCAGCTGGaa                                                                                   >  2:5254181/1‑150 (MQ=255)
                                                                     ccATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                                                                <  2:1691330/150‑1 (MQ=255)
                                                                     cattttttttAATTTTAGCCAATTGGATAAATGGTAAAGTGTGATATATTACACACTGACAAAAGAATAAGACAAATTAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                                                                <  2:1834191/147‑1 (MQ=255)
                                                                       aTTTTTTTTCTTTTAGCCTAGTGTATAAATGGGAATGTGGGAGCTATGACACAGAGAGAAATGAAGAAGATAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                                                              <  2:2158927/150‑1 (MQ=255)
                                                                       aTTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGCCACAGAGAGAAAAGAACAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCa                                                                              <  2:3181652/150‑1 (MQ=255)
                                                                              tACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTc                                                                       >  1:7567956/1‑150 (MQ=255)
                                                                               aatttttGCCAAGTGTATAAATGGTAATGTGGGATATATCACACAGAGACAAAATAAGAACATAAAACAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCa                                                                      <  1:7976315/147‑1 (MQ=255)
                                                                                aaTTTAGCCACGGGTAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAATAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAg                                                                     <  1:10011679/149‑1 (MQ=255)
                                                                                    tAGCCATGTGTATAAATGGGAAAGTGGGAGCTATGACACAGAGAGAAAACAATAAGACAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTc                                                                 <  1:6336432/150‑1 (MQ=255)
                                                                                      gCCATGTGGATAAAAGGTAATGTGGGAGCTATTACACAGAGAGAAAAGAAGAAGACAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                                                               <  2:4573591/150‑1 (MQ=255)
                                                                                      gCCATGGGGATAACAGGTAAAGTGGGAGCTATGACACAGAGAGAAAATAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                                                               <  2:886393/150‑1 (MQ=255)
                                                                                       ccACGTGGAGAAAAGGGAAAGTGGGAGCTATTACACAGACACAAAATAATAATATAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                                                              <  1:4741835/150‑1 (MQ=255)
                                                                                       ccACGTGGAGAAAAGGGAAAGTGGGAGCAATGACACAGACAGAAAAGACGCCGACAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCa                                                              <  2:23078/150‑1 (MQ=255)
                                                                                          cgTGGATAAAAGGTAAAGTGGGTGTTCTGACACAGTGAGAACAGATGACGAGAAAAGAAATCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGc                                                           <  1:9909195/149‑1 (MQ=255)
                                                                                          aGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGc                                                           <  2:8259334/150‑1 (MQ=255)
                                                                                            tGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaa                                                         >  1:1428809/1‑150 (MQ=255)
                                                                                              gagaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGGACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaaa                                                       <  1:6726497/150‑1 (MQ=255)
                                                                                                gaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt                                                     >  1:6443070/1‑150 (MQ=255)
                                                                                                     gggAACGTGGGAGCTATGCCCCAGAGAGAAAAGACGAAGAGAAAAGAAAACGCCCCTGCCAGCACCTGACAGGGGCGTACGTACCCGAGAGGAATTACCGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt                                                <  1:708666/150‑1 (MQ=255)
                                                                                                     gggAAAGTGGGAGCTATGCCACATACACAAAAGAACAACAGAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAAt                                                <  1:1116944/150‑1 (MQ=255)
                                                                                                      tgAATGTGGTAGATATTACACATATATAAAATAAGAAGATAAAATAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc                                               <  1:7874283/149‑1 (MQ=255)
                                                                                                      ggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACACAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATc                                               >  2:305287/1‑150 (MQ=255)
                                                                                                       gAAAGTGGGAGCTATGACACAGAGATAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCg                                              <  2:7265423/150‑1 (MQ=255)
                                                                                                        attgTGGTAGCTATGACACAGCGAGAAAAGAAGAAGACAACAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa                                             <  1:8708155/147‑1 (MQ=255)
                                                                                                        attgTGGGAGCTATCACACATAGATAAAAGAAGAACACAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa                                             <  1:3788163/147‑1 (MQ=255)
                                                                                                        aatgTGGGAGCTATGACCCAGCGAGAAACTAAGAAGACAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGa                                             <  1:3739413/147‑1 (MQ=255)
                                                                                                         aaGTGGGAGATAACAAACACATAAAAAACAACAACACAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGat                                            <  1:8935244/150‑1 (MQ=255)
                                                                                                             gggAGCTATGACACAGAGAGAAAAGAAGAAGACAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc                                        <  2:7737316/150‑1 (MQ=255)
                                                                                                                  cTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGgcg                                   >  2:4760767/1‑150 (MQ=255)
                                                                                                                    aTGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGca                                 >  1:5491768/1‑150 (MQ=255)
                                                                                                                    aTGACACAGAGAGAAAAGAAGAAGACAAAATAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGca                                 <  1:3178040/150‑1 (MQ=255)
                                                                                                                     tGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGcac                                >  1:9587777/1‑150 (MQ=255)
                                                                                                                         acaGAGGGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCg                            >  1:3911663/1‑150 (MQ=255)
                                                                                                                         acaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCg                            >  1:4566850/1‑150 (MQ=255)
                                                                                                                              gataaaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGa                       <  1:7693164/147‑1 (MQ=255)
                                                                                                                              cacaaaaCAACAACACAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGa                       <  1:9840805/147‑1 (MQ=255)
                                                                                                                                 aaaaGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGActc                    >  1:3578860/1‑150 (MQ=255)
                                                                                                                                   cccaagacgaGCAACGAAAACGCCCCTGCCAGCCACCGACCGGGGCGTACGTCCCCGAGAGGACATCACCCCGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCtt                  <  2:10118881/147‑1 (MQ=255)
                                                                                                                                   aataagacgaTAAAATAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCtt                  <  2:4348174/147‑1 (MQ=255)
                                                                                                                                   aagaacacgACAAAACAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCtt                  <  1:1019646/150‑1 (MQ=255)
                                                                                                                                    agaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGCTATCCAGGCGCACACCGTCAGACTCTTc                 >  1:4108848/1‑150 (MQ=255)
                                                                                                                                       agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGt              <  1:5003468/150‑1 (MQ=255)
                                                                                                                                        gaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTg             >  1:1800094/1‑150 (MQ=255)
                                                                                                                                         aagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGa            >  2:5496994/1‑150 (MQ=255)
                                                                                                                                         aagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGa            >  2:4166638/1‑150 (MQ=255)
                                                                                                                                         aagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGa            >  1:3362155/1‑150 (MQ=255)
                                                                                                                                           gagaAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa          <  1:576785/150‑1 (MQ=255)
                                                                                                                                           gagaAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa          >  2:9679143/1‑150 (MQ=255)
                                                                                                                                           gacaaaacaaaacGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGaaa          <  2:6290792/147‑1 (MQ=255)
                                                                                                                                            agaaaagaaaACGCCCCTGCCAGCAACTGACACGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAg         >  1:2387747/1‑150 (MQ=255)
                                                                                                                                              aaaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTc       >  2:3893848/1‑150 (MQ=255)
                                                                                                                                                aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg     <  1:8344744/150‑1 (MQ=255)
                                                                                                                                                aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg     >  1:7033274/1‑150 (MQ=255)
                                                                                                                                                aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg     >  2:7489949/1‑150 (MQ=255)
                                                                                                                                                aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTAAGTGAAAGTCgg     >  1:9263697/1‑150 (MQ=255)
                                                                                                                                                  gaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgggg   <  2:4010761/150‑1 (MQ=255)
                                                                                                                                                   aaatCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGt  >  2:7178653/1‑150 (MQ=255)
                                                                                                                                                   aaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGt  <  1:48073/150‑1 (MQ=255)
                                                                                                                                                   aaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGt  >  2:1370746/1‑150 (MQ=255)
                                                                                                                                                   aaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGt  >  2:535293/1‑150 (MQ=255)
                                                                                                                                                   aaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGt  >  1:1038823/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TAAAACCTGACCCATAACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGT  >  NC_000913/2460455‑2460751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: