Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,481,750 C→A 9.2% A143S (GCT→TCT)  emrY ← putative multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,481,7500CA 9.2% 1732.4 / 31.3 536A143S (GCT→TCT) emrYputative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (245/225);  new base A (2/64);  total (247/289)
Fisher's exact test for biased strand distribution p-value = 1.33e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 539 total aligned reads displayed.

TAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAA  >  NC_000913/2481601‑2481898
                                                                                                                                                     |                                                                                                                                                    
   ggTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCaaa                                                                                                                                                   >  2:5437125/1‑150 (MQ=255)
   ggTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCaaa                                                                                                                                                   <  2:2577116/150‑1 (MQ=255)
    gTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAAt                                                                                                                                                  >  1:956159/1‑150 (MQ=255)
    gTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAAt                                                                                                                                                  <  1:2733767/150‑1 (MQ=255)
    gTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAAt                                                                                                                                                  <  1:2650651/150‑1 (MQ=255)
     ttAATCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATg                                                                                                                                                 <  2:8366911/150‑1 (MQ=255)
     ttAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATg                                                                                                                                                 >  2:6908592/1‑150 (MQ=255)
            tAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCtttt                                                                                                                                          <  2:92864/150‑1 (MQ=255)
               tGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTc                                                                                                                                       >  1:8278130/1‑150 (MQ=255)
               tGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTc                                                                                                                                       >  1:5620869/1‑150 (MQ=255)
                gTCAGCACGATAATCCCCATAGGGACATTGAATAAAAATATCCAACCCCAGCTAAACTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCTATAATCACGGACATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCt                                                                                                                                      <  1:4917730/150‑1 (MQ=255)
                     gACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCgg                                                                                                                                 >  1:819571/1‑150 (MQ=255)
                     gACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCAGAATGCCAGAGCAAATGTTCTATTTTCTGGCgg                                                                                                                                 >  1:4712498/1‑150 (MQ=255)
                         aTAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATaa                                                                                                                             <  1:4023751/150‑1 (MQ=255)
                            aTCCCCATAGGGACATTGATTAAAAATATCCACCCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTc                                                                                                                          <  2:6785549/150‑1 (MQ=255)
                            aTCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTc                                                                                                                          >  2:3144090/1‑150 (MQ=255)
                            aTCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTc                                                                                                                          <  1:7204087/150‑1 (MQ=255)
                            aTCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTc                                                                                                                          <  1:10041835/150‑1 (MQ=255)
                             tCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTcc                                                                                                                         <  2:8506405/150‑1 (MQ=255)
                              ccccATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCt                                                                                                                        <  2:9606250/150‑1 (MQ=255)
                              ccccATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCt                                                                                                                        <  1:7621669/150‑1 (MQ=255)
                              ccccATAGGGACATTGATTAAAAATATCCAACCCCACCTAAACTTATCACAAATATAACCGCCCAATATCGCCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCt                                                                                                                        <  1:8158290/150‑1 (MQ=255)
                               cccATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCtt                                                                                                                       <  2:1610634/150‑1 (MQ=255)
                               cccATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCtt                                                                                                                       <  2:3003389/150‑1 (MQ=255)
                                ccATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATCTCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTta                                                                                                                      <  1:3855180/150‑1 (MQ=255)
                                ccATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTta                                                                                                                      >  2:5619804/1‑150 (MQ=255)
                                ccATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTta                                                                                                                      >  1:3045345/1‑150 (MQ=255)
                                   tAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaata                                                                                                                   <  2:5808714/150‑1 (MQ=255)
                                     gggACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaataat                                                                                                                 <  2:1052440/150‑1 (MQ=255)
                                      ggACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTtaataata                                                                                                                <  2:2480308/150‑1 (MQ=255)
                                          aTTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAAct                                                                                                            >  2:3335978/1‑150 (MQ=255)
                                          aTTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAAct                                                                                                            >  2:6228722/1‑150 (MQ=255)
                                          aTTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCAAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAAct                                                                                                            >  1:189952/1‑150 (MQ=255)
                                             gATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCtg                                                                                                         <  2:39159/150‑1 (MQ=255)
                                                   aaaTATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAg                                                                                                   >  2:4671722/1‑150 (MQ=255)
                                                   aaaTATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAg                                                                                                   >  1:5940117/1‑150 (MQ=255)
                                                    aaTATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGt                                                                                                  <  2:1776614/150‑1 (MQ=255)
                                                    aaTATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGACCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGt                                                                                                  <  2:4826470/150‑1 (MQ=255)
                                                     atatCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTg                                                                                                 <  1:8910573/150‑1 (MQ=255)
                                                        tCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGaa                                                                                              >  2:8969867/1‑150 (MQ=255)
                                                        tCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGaa                                                                                              <  1:3483373/150‑1 (MQ=255)
                                                             ccccAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAAc                                                                                         <  2:4088572/150‑1 (MQ=255)
                                                             ccccAGCGACAGGTATCACACATATACCCGCCCACGAGCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATATTGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAAGTAAc                                                                                         <  2:3286717/150‑1 (MQ=255)
                                                              cccAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACg                                                                                        >  1:2072209/1‑150 (MQ=255)
                                                                   cTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGcccc                                                                                   >  1:1340480/1‑150 (MQ=255)
                                                                    tAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCg                                                                                  <  2:2237524/150‑1 (MQ=255)
                                                                     aaaGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGc                                                                                 <  2:3072575/150‑1 (MQ=255)
                                                                            tCACAACTATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAc                                                                          <  2:2818211/150‑1 (MQ=255)
                                                                            tCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAc                                                                          <  2:7986231/150‑1 (MQ=255)
                                                                              acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                        >  1:788662/1‑150 (MQ=255)
                                                                              acaAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTCATTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAccc                                                                        <  1:6100525/150‑1 (MQ=255)
                                                                               caAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAAcccc                                                                       >  2:3616341/1‑150 (MQ=255)
                                                                                   tataCCCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAAGGCCAGAGCAAATGTTATTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGaa                                                                   <  1:8717934/150‑1 (MQ=255)
                                                                                       aCCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGAc                                                               >  1:9237898/1‑150 (MQ=255)
                                                                                         cGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGActc                                                             >  2:8706165/1‑150 (MQ=255)
                                                                                          gCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGActct                                                            >  2:4319261/1‑150 (MQ=255)
                                                                                           cccAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTa                                                           >  2:9062362/1‑150 (MQ=255)
                                                                                             cAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGGCAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaaa                                                         >  1:9683880/1‑150 (MQ=255)
                                                                                             cAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaaa                                                         >  2:5231699/1‑150 (MQ=255)
                                                                                                   cGGCCCACTTTTGGGAGCTATAATCCCGGTCTTTGACCATAATGCCTGTGCAATTGTTCTTTTTTGTGGGGTATATTTCCTTAATAATAAACTGTGTGACTGTGTATTTAAGGGCCCGGCCTTTAACCCCTGAACGACTCTAAAAAatat                                                   <  2:2712444/150‑1 (MQ=255)
                                                                                                    ggCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATc                                                  >  2:7638674/1‑150 (MQ=255)
                                                                                                     gCCCAAATAACGGAGCGATAATCACGGACATTGACAAAAATGCCAGAGAAAATTTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTTCAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCa                                                 <  2:8209432/150‑1 (MQ=255)
                                                                                                     ccccACATTACGGACCGAAAATCACGTTCTTTTACAATAACGCCAGACCAAATGTTCTTTTTTCTGGCGTAAATTCCCTTAATATTAAAATCTGTTACAGTGTAATTAACGGCCCCGCAATAAACCCCTCAACGACTCTAAAAAATATCa                                                 <  2:3520596/149‑1 (MQ=255)
                                                                                                         acaTATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGcac                                             >  1:8439076/1‑150 (MQ=255)
                                                                                                             atCGTAGCTATAATCACGGTCATTGACAATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCATTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCa                                         <  2:2432649/150‑1 (MQ=255)
                                                                                                                ggAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGACTTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGa                                      >  1:6246153/1‑150 (MQ=255)
                                                                                                                ggAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGa                                      >  1:1580866/1‑150 (MQ=255)
                                                                                                                 gAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGAt                                     >  1:5298844/1‑150 (MQ=255)
                                                                                                                  tgctaTAATCCCGTACATTTACAAAAATGCCTGATAAATTTTTATTTTTTGTGGGTTAAAATCCCTTAATAATAAACTCTGTTACTGTTTATTTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGAtt                                    <  1:9768294/146‑1 (MQ=255)
                                                                                                                  tgctaTAATCACTTTATTTTACAATAATGCCTGAGAAATTTTTCTTTTTTCTGGGGTATAATTCCTTAATAATAAACTCTGTGACTGTGTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGAtt                                    <  1:6984880/146‑1 (MQ=255)
                                                                                                                  aGCTATAATCCCGGAATTTGACCATAATGCCATAGCAATTTTTCTTTTTTCTGGCGGATATTTCCTTAATAATAAACTCTGTGACTGTGGAATTAAGGGCCCCGCATTTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGAtt                                    <  1:5799472/150‑1 (MQ=255)
                                                                                                                    cGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTgg                                  >  2:7147516/1‑150 (MQ=255)
                                                                                                                     tataataACGGTAATTGAACATAATGAAAGAGAAACTGTTCTTTTTCCTGGCGTATAATTCATTAATAATAAACTCTTTTACTGTGTAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                 <  2:6638222/149‑1 (MQ=255)
                                                                                                                     taTAATCAGGGTATTTGACAATATTGCCTGAGAATTTGTTATTTTTTCGGGGTTATATTTCTTTAATAATAAACGGTGTTACTGTGTAATTAACGGCCCCGCATTTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                 <  1:4532178/149‑1 (MQ=255)
                                                                                                                     taTAATCACGGTAATTGACAATAAGGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATACATTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                 <  1:6377091/149‑1 (MQ=255)
                                                                                                                     gATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                 >  2:4207286/1‑150 (MQ=255)
                                                                                                                     gATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                 >  2:4926452/1‑150 (MQ=255)
                                                                                                                     gATAATCACGGTATTTGACCTTAATGCCAGAGCAAATTTTCTTTTTTGTGGCGTATAATTCCTTAATAATAAACTCTGTGACAGTGTATTTAACGGCCCCGCCTTTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGt                                 <  2:5297750/150‑1 (MQ=255)
                                                                                                                      aTAATCACGTTCTTTCAACATAATCCCAGACCAAATTTTATTTTTTCTGGCGTATAATTCCTTAATAATAAACTCTTTTACAGTTTAATTAACGGCCCCGCATTTAACCCCTCAACGACTCTAAAAAATATCAGCACATCAAGATTGGTc                                <  1:3362454/150‑1 (MQ=255)
                                                                                                                      aTAATCACGTTAATTGACAATAATGCCAGAGAAATTTTTCTTTTTTCTGGCGTATATTTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTc                                <  2:7313494/150‑1 (MQ=255)
                                                                                                                        aaTCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGa                              >  1:9950020/1‑150 (MQ=255)
                                                                                                                        aaTCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGa                              >  1:8526289/1‑150 (MQ=255)
                                                                                                                         aTCACGGTAATTGACAATATTGCCAGAGCAATTTTTTTTTTTTGTGGCTGATAATTCCTTATTATTAACCTCTGTGACAGTGTATTTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGAt                             <  2:7812603/150‑1 (MQ=255)
                                                                                                                             cgttctttGACCATATTGCCAGAGAATTTTTTCTTTTTTCTGGGGTATATTTAATTAATAATAAACTCTTTGACTGTGTAATTAACGGCCCCGCCATTAACCCCTTAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGc                         <  2:2071864/144‑1 (MQ=255)
                                                                                                                             cGGTCATTGACAATAATGCCTGAGAAAATGTTCTTTTTTCTGGCGTATATTTCCTTAATAATAAACTCTGTGACAGTGTAATTAACGGCCCCGCAATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGc                         <  2:6318270/150‑1 (MQ=255)
                                                                                                                               taaaTTCACCATAATCCCATAGCAATTTTTGTTTTTTCTGGCGTATAATTCCTTAAAAATAAACTCGGTGACAGTGGAATTAACGGCCCCGCCATTACCCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTa                       <  1:248270/147‑1 (MQ=255)
                                                                                                                               gTCATTGACCATACTGCCAGAGAAAATTTTCTTTTTTCGGGCGTATATTACCTTAATAATAAACTCTTTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTa                       <  1:5450274/150‑1 (MQ=255)
                                                                                                                               gTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTa                       >  1:7407767/1‑150 (MQ=255)
                                                                                                                                  aTTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTccac                    >  1:6547496/1‑150 (MQ=255)
                                                                                                                                  aTTGACAATAAGGCCAGAGAAATTGTTCTTTTTTCTGGCGGATAATTCCTTAATAAAAAACTCTGTGACAGGGCAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTacac                    <  1:2360286/150‑1 (MQ=255)
                                                                                                                                   ttGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTAcaca                   >  1:3272844/1‑150 (MQ=255)
                                                                                                                                   ttGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTAcaca                   >  2:1015871/1‑150 (MQ=255)
                                                                                                                                   ttGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTAcaca                   >  2:9738367/1‑150 (MQ=255)
                                                                                                                                           attGCCAGAGAATTTTTTGTTTTTTGTGGGGTATATTTCCTTAATAATAAACTCTGTTACTGGGGATTTAACGGCCCCGCATTTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGaa           <  2:4426306/148‑1 (MQ=255)
                                                                                                                                           aaTGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGaa           <  2:5940117/150‑1 (MQ=255)
                                                                                                                                             tGCCAGAGCAATTGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACTGTGTAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGa         <  1:3713840/150‑1 (MQ=255)
                                                                                                                                               ccAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACa       >  1:4292540/1‑150 (MQ=255)
                                                                                                                                                cAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAg      >  2:3981209/1‑150 (MQ=255)
                                                                                                                                                  gagCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCg    >  1:2190557/1‑150 (MQ=255)
                                                                                                                                                   agCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGa   >  2:1491243/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAA  >  NC_000913/2481601‑2481898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: