Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,718,314 A→C 5.3% V72G (GTT→GGT)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,718,3140AC 5.3% 1877.2 / 17.7 621V72G (GTT→GGT) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (361/210);  new base C (4/45);  total (365/256)
Fisher's exact test for biased strand distribution p-value = 1.29e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 650 total aligned reads displayed.

TCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAA  >  NC_000913/2718165‑2718463
                                                                                                                                                     |                                                                                                                                                     
tctGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGaaa                                                                                                                                                       >  2:3815352/1‑150 (MQ=255)
  tGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAcc                                                                                                                                                     >  2:9848655/1‑150 (MQ=255)
   ggAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                                                                                                    <  1:10143548/150‑1 (MQ=255)
   ggAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                                                                                                    >  2:5766640/1‑150 (MQ=255)
   ggAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                                                                                                    >  2:4335120/1‑150 (MQ=255)
       aaGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTgcg                                                                                                                                                <  2:176373/150‑1 (MQ=255)
        aGTGCCTGACAGGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTCCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTgcgc                                                                                                                                               <  2:6647160/150‑1 (MQ=255)
         gTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGcc                                                                                                                                              >  1:7934653/1‑150 (MQ=255)
          tGCCTGACATGCATTTTCGCCATAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCa                                                                                                                                             >  1:9823668/1‑150 (MQ=255)
                  aTGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAAt                                                                                                                                     >  2:9524496/1‑150 (MQ=255)
                   tGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATc                                                                                                                                    >  2:8820562/1‑150 (MQ=255)
                    gCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCt                                                                                                                                   >  2:8546585/1‑150 (MQ=255)
                    gCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCt                                                                                                                                   >  2:1016042/1‑150 (MQ=255)
                    gCATTTTCACCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCt                                                                                                                                   >  1:894944/1‑150 (MQ=255)
                        tttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTccc                                                                                                                               >  2:5497616/1‑150 (MQ=255)
                         ttCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCa                                                                                                                              >  1:6558477/1‑150 (MQ=255)
                            gCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt                                                                                                                           >  1:3215867/1‑150 (MQ=255)
                               gTAGACGCGGTTTACTCCCGCACGCTGACGACGAAACACTTCCGTAAAAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCGGGCGTATCACCACCGGGCGCGGGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg                                                                                                                        <  2:3944460/150‑1 (MQ=255)
                                  gACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTc                                                                                                                     >  2:7963143/1‑150 (MQ=255)
                                  gACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTc                                                                                                                     >  2:6259040/1‑150 (MQ=255)
                                     gcgGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACg                                                                                                                  >  1:1594044/1‑150 (MQ=255)
                                       gggTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg                                                                                                                >  2:5181604/1‑150 (MQ=255)
                                        ggTTTCTCCCGCCCGCGGCCGCCGCAACACGGCCGGATCAATAAAACTTTTCCCACTGCGCCCCCGGTGAGGGGCCTTTTCCGGGGTCTCCCCCCCCGGCGCGCGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg                                                                                                               <  2:8079574/150‑1 (MQ=255)
                                         ttttcgaccGCACGCTGACGACGCACAACTGCCGTAAAAAAAAAACTTTTACCACGGAGCCCACCTTGTTCGGCATTTTCCGGCGTATCCTCACCCGGGGCGGGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGc                                                                                                              <  2:2628165/143‑1 (MQ=255)
                                          tttCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCt                                                                                                             >  2:280271/1‑150 (MQ=255)
                                           ttcttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTg                                                                                                            >  2:1064349/1‑150 (MQ=255)
                                            tcttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTCCCACTGATGCCACGGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa                                                                                                           <  2:1631290/150‑1 (MQ=255)
                                             cttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc                                                                                                          >  2:9290638/1‑150 (MQ=255)
                                              ttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct                                                                                                         >  2:6327369/1‑150 (MQ=255)
                                              ttcCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct                                                                                                         >  1:3932732/1‑150 (MQ=255)
                                                ccGCACGCTGACGACGAAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct                                                                                                       <  2:644867/150‑1 (MQ=255)
                                                 cGCACGCTGACGACGCAACACTTCCGGAACAATAAAACTTTTACCACTGATGCCACCGTGATGGGCCTTTTCCGGCGTCTCATCACCGGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTg                                                                                                      <  2:1209850/150‑1 (MQ=255)
                                                  gCACGCTGACGCCGAAACACTCCCGTATCAATAAAATTTTTCCCACTGATCCCACCGTTATGGGCCTTTCCCGGGTTATCATCACCGGGGGGGCGGCAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt                                                                                                     <  2:3492905/150‑1 (MQ=255)
                                                  gCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt                                                                                                     >  1:6410446/1‑150 (MQ=255)
                                                   cccGCTGACGCCGAAACACTCCCGGAACAATAAATATTTCACAACTGCTCCCCCGGTGCTCGGCCTTTCCGGGCGTCAACCCCCCCGGGGGGCGGTAACCGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt                                                                                                    <  2:7767180/148‑1 (MQ=255)
                                                   cccGCTGACGACGCAACACTCCCGGATCAATAAAACTTTTCCCCCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt                                                                                                    <  2:7038110/148‑1 (MQ=255)
                                                   cACGCTGACGACGAAACACTTCCGGACCAATAAAACTTTTCCCACTGAGCCCCCGGGGAGGGGCCTTTCCGGGCGTCCCCTCACCCGGCGCGCGGGAACCGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt                                                                                                    <  2:1007789/150‑1 (MQ=255)
                                                            gacgCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATcgc                                                                                           >  1:1847502/1‑150 (MQ=255)
                                                                 aaCACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg                                                                                      >  2:5249439/1‑150 (MQ=255)
                                                                 aaCACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTATTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg                                                                                      >  2:9724830/1‑150 (MQ=255)
                                                                       tCCGGATCAAACACACTTTTACCCCGGCGGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGa                                                                                <  2:9440990/150‑1 (MQ=255)
                                                                         cGGATCAATAAAACTTTTACCCCTGATGCCACGGTTATCGGCCTTTTCGGGCGTCTCATCCCCCGGCGCGCGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACg                                                                              <  1:7675936/150‑1 (MQ=255)
                                                                          ttaacAATAAATCTTTCACCCCTTATCCCCCGGTGTTCGGCCTTGACGGGCTTCACATCACCGGGCGGGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                                                             <  1:8770641/146‑1 (MQ=255)
                                                                          gtaaaaaTAAAACTTTTACCACTGATGCCACCGTGATGGGCCTTTTCCGGCGTCTCACCACCCGGCGCGCGGGAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                                                             <  2:7339318/145‑1 (MQ=255)
                                                                          ggATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                                                             >  1:1202378/1‑150 (MQ=255)
                                                                           gATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGc                                                                            >  1:4211550/1‑150 (MQ=255)
                                                                               aaTACACCTTTTACCACTGCCGCCCCCGTGATCGGCCTTTCCCGCCTTCTCAACCCCCGGCGGGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAcc                                                                        <  1:7227074/150‑1 (MQ=255)
                                                                               aaTAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAcc                                                                        >  2:3734156/1‑150 (MQ=255)
                                                                                 tAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGt                                                                      >  1:8609380/1‑150 (MQ=255)
                                                                                   aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCa                                                                    >  1:5512105/1‑150 (MQ=255)
                                                                                    aaattttACCACTGAGGCCCCCGTGAGCGGCCTTTTCCGGCGTCTCATCCCCCGGCGCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAg                                                                   <  2:8170697/147‑1 (MQ=255)
                                                                                       ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCAc                                                                >  1:8481202/1‑150 (MQ=255)
                                                                                       ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCAc                                                                >  2:4577183/1‑150 (MQ=255)
                                                                                       ttttACCACTGATGACACCGTGATCGGCCTTTTCCGGCGACTCATCACCCTGCGCGCGGGAAACCGTGAGCCACGGCGAATCTTCCCACTTGCGGACACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCAc                                                                >  2:9409255/1‑150 (MQ=255)
                                                                                           aCCACTGAGGCCACCGTGTTCGGCCTTTGCCGGCGTCTAATCCCCCGGCGCGGGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACgaga                                                            <  2:2218157/150‑1 (MQ=255)
                                                                                              aCTGATGCCACCGTGATCGGCCGTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAttt                                                         >  2:5865200/1‑150 (MQ=255)
                                                                                               ctgctgCCACCGTGATCGGCCTGTCCCGGCGTCACATCACCGGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtttt                                                        <  1:4852147/150‑1 (MQ=255)
                                                                                               cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtttt                                                        >  1:576801/1‑150 (MQ=255)
                                                                                               cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtttt                                                        >  2:6542051/1‑150 (MQ=255)
                                                                                               cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtttt                                                        >  1:4398156/1‑150 (MQ=255)
                                                                                                tGATCCCACCGTGATCGGCCTTTCCCGGCGTCCCACCACCCGGCGCGCGGGAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTc                                                       <  2:1808232/150‑1 (MQ=255)
                                                                                                  ttGCCACCGTGATCGGCCTTTCCCGGCGTCCCACCACCCGGCGCGCGGCAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCt                                                     <  1:9984365/149‑1 (MQ=255)
                                                                                                     ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGa                                                  <  1:9562094/150‑1 (MQ=255)
                                                                                                       aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCGTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcg                                                >  2:3168620/1‑150 (MQ=255)
                                                                                                          gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgccc                                             <  1:4903818/150‑1 (MQ=255)
                                                                                                          gTGACCGGCCTTTCCCGGCGTCACACCACCCGGCGCGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgccc                                             <  1:2692947/150‑1 (MQ=255)
                                                                                                             aTCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgcc                                          >  1:2324194/1‑150 (MQ=255)
                                                                                                             aTCGGCCTTTTCCGGCGTCTCAACACCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgcc                                          <  1:5291180/150‑1 (MQ=255)
                                                                                                              tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCGGGACgcccgccc                                         >  1:9256283/1‑150 (MQ=255)
                                                                                                                ggCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGccccc                                       >  2:875007/1‑150 (MQ=255)
                                                                                                                ggCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGccccc                                       >  1:2439397/1‑150 (MQ=255)
                                                                                                                ggCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGccccc                                       >  1:10047767/1‑150 (MQ=255)
                                                                                                                 gctttttCCGGCTTATCAACCCCGGGCGGGGGGTAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCt                                      <  2:4735513/147‑1 (MQ=255)
                                                                                                                 gCCTTTTCGGGGTTCTAACCCCCCGGCGCGGGGCAACCGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCt                                      <  2:2083868/150‑1 (MQ=255)
                                                                                                                    ttttCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc                                   >  2:4426858/1‑150 (MQ=255)
                                                                                                                    ttttCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc                                   <  1:6864484/150‑1 (MQ=255)
                                                                                                                     tttCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCATGAGATTTTCCTGGACGCCCGCCCCCTTTcc                                  >  1:1056932/1‑150 (MQ=255)
                                                                                                                         cGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGt                              >  2:5344876/1‑150 (MQ=255)
                                                                                                                           gCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAc                            >  1:368299/1‑150 (MQ=255)
                                                                                                                                tcatcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                       >  1:5975353/1‑150 (MQ=255)
                                                                                                                                tcatcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                       >  2:9638552/1‑150 (MQ=255)
                                                                                                                                tcatcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                       >  1:5077899/1‑150 (MQ=255)
                                                                                                                                tcatcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTGTCCCGGGACGCggg                       >  2:2382669/1‑150 (MQ=255)
                                                                                                                                 catcaCGCGGCGGGCGTGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCTGTACGCGGGt                      <  1:8239560/150‑1 (MQ=255)
                                                                                                                                 catcaCCCGGCGCGGGGGAACCGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                      <  1:4620490/150‑1 (MQ=255)
                                                                                                                                   tcccccGGGGCGGGGTAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                    <  2:6935554/147‑1 (MQ=255)
                                                                                                                                   tcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                    <  2:5981508/150‑1 (MQ=255)
                                                                                                                                    caCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgt                   <  1:1340905/150‑1 (MQ=255)
                                                                                                                                     aCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgtg                  <  2:3323807/150‑1 (MQ=255)
                                                                                                                                       ccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTggg                >  1:7751139/1‑150 (MQ=255)
                                                                                                                                         ggCGCGCGGTAAACCGCGCGCCACGGCGAATCTTCCCCCTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTGCCCGGTACGCGGGTTGTGGGTa              <  2:1425206/150‑1 (MQ=255)
                                                                                                                                          gcgcgcGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTAc             <  2:4118323/150‑1 (MQ=255)
                                                                                                                                           cgcgcgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTCCg            >  2:9162636/1‑150 (MQ=255)
                                                                                                                                                ggtaaaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt       <  1:3333929/147‑1 (MQ=255)
                                                                                                                                                gggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGTGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACTCGGGTTGTGGGTACGTTTAt       >  2:8653523/1‑150 (MQ=255)
                                                                                                                                                gggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt       >  2:8514230/1‑150 (MQ=255)
                                                                                                                                                gggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt       >  2:3017000/1‑150 (MQ=255)
                                                                                                                                                  gAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa     <  1:3052265/150‑1 (MQ=255)
                                                                                                                                                  gAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCa     >  1:2863790/1‑150 (MQ=255)
                                                                                                                                                    aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa   <  1:7394942/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTGGAAAAGTGCCTGACATGCATTTTCGCCGTAGACGCGGGTTTCTTCCGCACGCTGACGACGCAACACTTCCGGATCAATAAAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAA  >  NC_000913/2718165‑2718463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑