BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,049	T→A	6.1%	intergenic (‑189/+10)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,049	0	T	A	6.1%	1608.3 / 63.5	476	intergenic (‑189/+10)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (246/200); new base A (13/17); total (259/217)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 483 total aligned reads displayed.

ACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAT  >  NC_000913/2817900‑2818198

  taAAGCCATATCAATTAAGAGATTGTGCAGCCGGGATGATTACTCGGCTTGCGGCTCGCCCTTCGGGCCGTTGCTATAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                     <  1:3980091/150‑1 (MQ=17)

    aaGCGAAATCTATTTAGAGATGGTGCTTCAGGGAGGATTTCTCGCCTTGAGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                                                                                                   <  1:959865/150‑1 (MQ=17)

       ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                                >  1:1188509/1‑150 (MQ=255)

           atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTACAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATG                                                                                                                                            <  1:3144065‑M1/150‑1 (MQ=255)

              aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTG                                                                                                                                         >  1:8518205‑M1/1‑150 (MQ=255)

               aTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTTGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGC                                                                                                                                        <  2:4997999‑M1/150‑1 (MQ=255)

               aTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGC                                                                                                                                        >  1:3275001‑M1/1‑150 (MQ=255)

                   agagATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCACCC                                                                                                                                    >  1:6322511‑M1/1‑150 (MQ=255)

                    gagaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAAGATGGTGCATCCG                                                                                                                                   >  1:9496794‑M1/1‑150 (MQ=255)

                     agaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGG                                                                                                                                  <  1:4665498‑M1/150‑1 (MQ=255)

                       aTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGA                                                                                                                                <  2:3017946‑M1/150‑1 (MQ=255)

                         ggTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGG                                                                                                                              >  1:9994715‑M1/1‑150 (MQ=255)

                          gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGA                                                                                                                             <  2:1021444‑M1/150‑1 (MQ=255)

                           tGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGAT                                                                                                                            <  1:9182169‑M1/150‑1 (MQ=255)

                            gCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATT                                                                                                                           <  2:3954599‑M1/150‑1 (MQ=255)

                               tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGa                                                                                                                        <  1:5939091/150‑1 (MQ=255)

                                ccGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGaa                                                                                                                       >  2:5542926/1‑150 (MQ=255)

                                 cGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAc                                                                                                                      <  2:5045145/150‑1 (MQ=255)

                                  gggAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAAcc                                                                                                                     >  1:1848789/1‑150 (MQ=255)

                                       gaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGa                                                                                                                >  2:1404435/1‑150 (MQ=255)

                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCAGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                          >  1:1623385/1‑150 (MQ=255)

                                               gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCAGCGGTTAAATCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCgg                                                                                                        >  1:9803426/1‑150 (MQ=255)

                                                 cTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                                                                      >  2:1712646/1‑150 (MQ=255)

                                                  ttGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                                                                     <  2:5358444/150‑1 (MQ=255)

                                                     cGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                                  <  1:2978673/150‑1 (MQ=255)

                                                      ggCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                                                                 >  2:189815/1‑150 (MQ=255)

                                                      ggCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCAACCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAg                                                                                                 >  2:2010182/1‑150 (MQ=255)

                                                        ttcGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTG                                                                                                                                         >  2:688123‑M1/2‑108 (MQ=255)

                                                        cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCCTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                                                                                               >  2:7510810/1‑150 (MQ=255)

                                                          cGCCCTTCGGGCCTTTGCTAACGCAACGTTATCATCCGTAGTGCCTGCGGTTTTTTCCGCTACCTCTTAGTTACCTTCTGTTCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                             <  1:8259635/150‑1 (MQ=14)

                                                          cGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                             >  2:4337842/1‑150 (MQ=255)

                                                          cGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAGTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                             <  2:9731110/150‑1 (MQ=255)

                                                              cTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGt                                                                                                       <  1:4923946/136‑1 (MQ=255)

                                                              cTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTACGCTACCTCTTCGATACCTTCATTGCTGAAGATTTAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAc                                                                                         >  1:573317/1‑150 (MQ=255)

                                                                   ggCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTAt                                                                                    >  2:4595438/1‑150 (MQ=255)

                                                                     acGTTGCTTTAGCAACGTTATCATCCCAAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                                                                  >  1:8410005/2‑150 (MQ=255)

                                                                        ttGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc                                                                               >  2:7264485/1‑150 (MQ=255)

                                                                         tGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGGGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                              <  1:6752509/150‑1 (MQ=255)

                                                                         tGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCt                                                                              <  1:774270/150‑1 (MQ=255)

                                                                           cTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                            <  1:4403255/150‑1 (MQ=255)

                                                                           cTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTAAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa                                                                            >  1:7469644/1‑150 (MQ=255)

                                                                            tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGAACTCTATCCAGCTGAg                                                                           >  1:3745928/1‑150 (MQ=255)

                                                                            tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTAGAACCACCGACCGCGCGGATCGTAGCCGAATACTCTATCCATCttat                                                                           >  1:5099660/1‑147 (MQ=17)

                                                                             aaaGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTactcgccttgcggctcgcccttcgggccgttg                                                                                           >  1:3364171‑M1/1‑101 (MQ=255)

                                                                              aaGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                                <  2:9032996/79‑1 (MQ=255)

                                                                              aaGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                                                                                                >  1:9032996/1‑79 (MQ=255)

                                                                              aaGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                                         >  1:2472917/1‑150 (MQ=255)

                                                                               aGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                                                        >  1:2103301/1‑150 (MQ=255)

                                                                                   aCGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                                                                    <  2:8431820/150‑1 (MQ=255)

                                                                                     gTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                                  >  2:6516182/1‑150 (MQ=255)

                                                                                        atcatcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAt                                                               >  1:5871059/1‑150 (MQ=255)

                                                                                          catcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                             <  1:7044996/150‑1 (MQ=255)

                                                                                           atcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                                                            >  1:3577025/1‑150 (MQ=255)

                                                                                           atcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                                                            >  1:6604571/1‑150 (MQ=255)

                                                                                             cccTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                             >  1:5491739/1‑147 (MQ=255)

                                                                                                      cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttattt                                                 <  1:7032814/150‑1 (MQ=255)

                                                                                                      cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCACGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttagtt                                                 >  2:8048223/1‑150 (MQ=255)

                                                                                                         cGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGGTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTATTTTAc                                              >  2:2206427/1‑150 (MQ=255)

                                                                                                            ttAATTCCGCTATCTCTTCGATACCTTCATTGCTGAAGCTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgc                                           <  1:6638370/150‑1 (MQ=255)

                                                                                                             tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                          <  2:2631040/150‑1 (MQ=255)

                                                                                                               aTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGa                                        <  1:1404435/150‑1 (MQ=255)

                                                                                                                ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGACATTTATTTTACTGCTGat                                       >  1:8771131/1‑150 (MQ=255)

                                                                                                                ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGat                                       <  1:8141992/150‑1 (MQ=255)

                                                                                                                 tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGatc                                      >  1:5257351/1‑149 (MQ=255)

                                                                                                                  ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGatat                                     <  2:3818593/150‑1 (MQ=255)

                                                                                                                   cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                                    >  1:5570764/1‑150 (MQ=255)

                                                                                                                   cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGATAtt                                    >  2:6371608/1‑150 (MQ=255)

                                                                                                                      tcccTATTCGATACCTTCTTTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGAt                                 <  1:96576/148‑1 (MQ=255)

                                                                                                                      tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGAt                                 <  1:10006815/150‑1 (MQ=255)

                                                                                                                       aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTACTGCTGATATTGATa                                >  2:9286933/1‑150 (MQ=255)

                                                                                                                        ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAc                               <  2:4235388/150‑1 (MQ=255)

                                                                                                                        ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATAc                               <  2:4747257/150‑1 (MQ=255)

                                                                                                                         ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGAttttttgata                              <  2:4877757/150‑7 (MQ=11)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTa                                               <  2:1565803/132‑1 (MQ=17)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                             >  2:5715414/1‑150 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                             >  2:6127490/1‑150 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTACTGCTGATATTGATACCg                             >  2:2525216/1‑150 (MQ=255)

                                                                                                                          tctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGATATTTATACCg                             >  2:7344611/1‑150 (MQ=255)

                                                                                                                           ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGc                            >  1:6522868/1‑150 (MQ=255)

                                                                                                                            ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACAGCTGGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTATTTTAATGATGATATTTATACCGct                           >  2:8675130/1‑150 (MQ=11)

                                                                                                                             tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                          <  1:9314916/150‑1 (MQ=255)

                                                                                                                             tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                          >  1:7590702/1‑150 (MQ=255)

                                                                                                                              cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctac                         >  1:4980962/1‑150 (MQ=255)

                                                                                                                               gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGATATTGATAACGctact                        >  2:2931563/1‑150 (MQ=255)

                                                                                                                                  aCCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                     <  1:2589443/150‑1 (MQ=255)

                                                                                                                                  aCCTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                     <  1:1762494/150‑1 (MQ=25)

                                                                                                                                  aCCTTCATTGCTGAAGCTTTCTTAGAATATGGTGAATCCTGTAGGATTCGACCCTACGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                     <  2:1143094/150‑1 (MQ=14)

                                                                                                                                  aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                     <  1:7946836/150‑1 (MQ=255)

                                                                                                                                  aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTaaa                     <  2:7962405/150‑1 (MQ=255)

                                                                                                                                    cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGa                                                                                                                        <  1:4753395/49‑1 (MQ=255)

                                                                                                                                    cTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGc                   <  2:5929556/150‑1 (MQ=255)

                                                                                                                                       cATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCat                <  2:3568865/150‑1 (MQ=255)

                                                                                                                                       cATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCat                <  2:6630683/150‑1 (MQ=255)

                                                                                                                                        aTTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATT                                                                                                                           <  1:6886135‑M1/42‑1 (MQ=255)

                                                                                                                                        aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGcta                          <  2:3355362/139‑1 (MQ=255)

                                                                                                                                            cTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCaa           <  1:8947334/150‑1 (MQ=255)

                                                                                                                                            cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCaa           <  1:10102554/150‑1 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc                                                                                                          <  2:9510556/54‑1 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg          >  2:9210475/1‑150 (MQ=255)

                                                                                                                                             tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAATTAGTTTACTGCTGATATTGATACCGCTCCTATAGCCCTCTCacg          >  2:709317/1‑148 (MQ=11)

ACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAT  >  NC_000913/2817900‑2818198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`