Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,046,068 T→A 15.6% intergenic (‑167/+100) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,046,0680TA15.6% 1243.9 / 59.8 501intergenic (‑167/+100)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base T (114/286);  new base A (82/4);  total (208/293)
Fisher's exact test for biased strand distribution p-value = 7.98e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 528 total aligned reads displayed.

ATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGC  >  NC_000913/3045919‑3046217
                                                                                                                                                     |                                                                                                                                                     
attaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCct                                                                                                                                                       <  2:2322529/150‑1 (MQ=255)
   aattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCtctc                                                                                                                                                    <  1:8391608/150‑1 (MQ=255)
   aattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCtctc                                                                                                                                                    <  1:4140103/150‑1 (MQ=255)
   aattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCACCCAAAACCactc                                                                                                                                                    >  1:14993/1‑150 (MQ=255)
        gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCGAACCCACACCCCaa                                                                                                                                               >  2:9773261/1‑150 (MQ=255)
         ttGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCGTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                              <  2:8831662/150‑1 (MQ=255)
              gTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACTCCCCCAAGGGGc                                                                                                                                         >  1:7213536/1‑150 (MQ=255)
                aTCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                                       <  1:1891285/150‑1 (MQ=255)
                   tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTGCCCCGCACCCGCAACCACACCCCCAAgggggggggg                                                                                                                                    >  1:3559135/1‑150 (MQ=255)
                        gaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgggttagg                                                                                                                               <  1:9901633/150‑6 (MQ=37)
                         aTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGACTGt                                                                                                                              >  2:5571563/1‑150 (MQ=255)
                          ttGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTc                                                                                                                             <  2:2762132/150‑1 (MQ=255)
                           tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCt                                                                                                                            <  1:1176415/150‑1 (MQ=255)
                           tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCt                                                                                                                            <  1:1001505/150‑1 (MQ=255)
                           tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAACCACTCCCCAAAGGGGCGCGGGGACTGTCt                                                                                                                            >  1:8769862/1‑150 (MQ=255)
                            gCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCGAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTg                                                                                                                           >  1:4791881/1‑150 (MQ=255)
                            gCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCAAAACCACAACCCAAAAGGGCGAGGTGCCTGTATg                                                                                                                           >  1:2199943/1‑150 (MQ=255)
                             cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                          <  1:5714525/150‑1 (MQ=255)
                             cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                          <  2:2048180/150‑1 (MQ=255)
                              tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAg                                                                                                                         >  2:10215872/1‑150 (MQ=255)
                              tAACAAACTGGCTCAATAATCAATGATGTCACGCTTTTCCCACTCGCCCTTTCAGGGAGAGGGCCGGGGTCAGGGTAAATATTCGCGCCAGTGAAGGCCTGTTACCCACAACCAAACCCACTACACAAAGGGGCGAGCGGACTGtattat                                                                                                                         >  2:8322320/1‑145 (MQ=255)
                                      tGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAAACCACAACCCCAAGGGGCGAGGGGACAGGCTGAGCAcagttg                                                                                                                 >  1:863212/1‑145 (MQ=255)
                                        gCTCAATCTGCACGGGGGGCCCGCGGTGCCCCCTCGCCCGGGCCGGGAGCGGGCCGGGGGGCGGGTAAAGCGTCGCGCCAGTGCCGGCCTGTTCCCCTCCCCCGAACCCGCGCCCCACAGGGGCGCGGGGACTGTCTGCGCACTTTTGTa                                                                                                               <  2:577928/150‑1 (MQ=255)
                                         cTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTACCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTAc                                                                                                              >  2:6720517/1‑150 (MQ=255)
                                         cTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCAAACCCACTCCCCAAAGGGGCGCGGGGACTGTCTGAGCCCTGTTGTAc                                                                                                              >  1:3238037/1‑150 (MQ=255)
                                          tCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCAAAACCACACCCCAAAAGGGGGAGGGCCCAGTAAGAGCCCGTTTGTaaa                                                                                                             >  1:10269950/1‑148 (MQ=255)
                                            aaTCTTCAATGTTGGCTCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                                           <  2:2601399/150‑1 (MQ=255)
                                                ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTGTGTACTTTGTCa                                                                                                       >  2:10141065/1‑150 (MQ=255)
                                                ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACAACCCAAAGGGGCGAGGGGACAGTGTGAGCCCGTTTGTAAATTGtga                                                                                                       >  1:8246207/1‑148 (MQ=255)
                                                  cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATc                                                                                                     <  1:4527335/150‑1 (MQ=255)
                                                   aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                                    <  1:6457443/150‑1 (MQ=255)
                                                   aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                                    <  2:406420/150‑1 (MQ=255)
                                                   aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                                    <  1:3868870/150‑1 (MQ=255)
                                                   aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                                    <  2:7377155/150‑1 (MQ=255)
                                                      gTTGGCACGCTGTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGAc                                                                                                 <  1:6166791/150‑1 (MQ=255)
                                                           cACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCGCCCCCCGACCCACTCCCCCCACGGGCGGGGGGTCTGTCTGAGGACCGTTGTGAGTTTTCATCTGTCTaaaa                                                                                            >  2:7770398/1‑150 (MQ=255)
                                                           cACGCTTGTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaa                                                                                            <  1:7117545/150‑1 (MQ=255)
                                                            aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                                                                                           >  2:10279961/1‑150 (MQ=255)
                                                            aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                                                                                           <  1:8027750/150‑1 (MQ=255)
                                                            aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCGCTCCCCCAAGGGGCGCGGGGACGGTCTGTGCACGTTTGTACTGTGTCATCTGACGaaaat                                                                                           >  2:5524135/1‑149 (MQ=25)
                                                             cGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAg                                                                                          <  1:6852206/150‑1 (MQ=255)
                                                                 tttCCCCCTCGCCCTGTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcgc                                                                                      <  1:1642008/150‑1 (MQ=255)
                                                                  ccggcccTCTCCCTGAAAGGGCGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACTTTGTCATCTGAATAAAAAGGCGcc                                                                                     >  2:457771/5‑150 (MQ=21)
                                                                    cccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGa                                                                                   <  2:6541096/150‑1 (MQ=255)
                                                                     ccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGaa                                                                                  <  2:1009205/150‑1 (MQ=255)
                                                                       ccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgc                                                                                <  2:5256200/150‑1 (MQ=255)
                                                                         tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACCCCCAAACCCACTCCCCCAAGGGGCGGGGGGCCTGTCTGAGGACTTGTGTTCGTTGTAATAATAAAAAAAAGGCGCCGGAgcgc                                                                              >  1:236540/1‑150 (MQ=255)
                                                                         tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCAAACCCACAACCCCAATGGGGGGTGGGACTGAGTGAGCCATGTTTTTCATTTTCATCAGAGTAAAAAGGCGCCGAAgcgc                                                                              >  1:7882351/1‑150 (MQ=255)
                                                                           gCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCt                                                                            >  2:9423291/1‑150 (MQ=255)
                                                                           gCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCt                                                                            >  2:2399502/1‑150 (MQ=255)
                                                                             ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCttt                                                                          <  2:3294499/150‑1 (MQ=255)
                                                                             ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCttt                                                                          <  2:8115742/150‑1 (MQ=255)
                                                                               tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCACACCCTAACCCTCAACCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCGTTAg                                                                        >  2:7874160/1‑150 (MQ=255)
                                                                               tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCCCCCGAACCCACTCCCCCAAGGGGCGCGGGGACTGGCGGAGCACGGTTGTACTTTGGCATCTGACTAAAAAGGCGCCGAACCGccggtag                                                                        >  2:3070020/1‑145 (MQ=255)
                                                                               ggtCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTGCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAg                                                                        <  1:7569809/148‑1 (MQ=255)
                                                                                ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGa                                                                       <  1:3721830/150‑1 (MQ=255)
                                                                                ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGa                                                                       <  1:5143703/150‑1 (MQ=255)
                                                                                ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGa                                                                       <  2:3899729/150‑1 (MQ=255)
                                                                                tgcAGGGAGAGGGCCGGGGTGAGGGAACATACTCCCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGa                                                                       <  1:3009508/148‑1 (MQ=255)
                                                                                   aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                                    <  2:4926360/150‑1 (MQ=255)
                                                                                   aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                                    <  1:42829/150‑1 (MQ=255)
                                                                                   aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTCACCCAAAACCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCCCTTTTGTACTTTGTCATCTTACTAAAAATGCGCCGAAGCGCCGTTAGaaaa                                                                    >  2:4773626/1‑150 (MQ=37)
                                                                                   aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCACACCCGAACCCACAACCCAAAGGGGCGCGGGGACGGTCTGAGCACTTTTGTACGTTGTGATCTTACAAAAAATGCGCCGCAGCGCCGTTATaaaa                                                                    >  1:9064843/1‑150 (MQ=255)
                                                                                     ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCAAAGCGCCTTTAGAAAATa                                                                  <  2:8683017/150‑1 (MQ=255)
                                                                                        gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc                                                               <  1:4912759/150‑1 (MQ=255)
                                                                                          gggCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGa                                                             <  2:9060236/150‑1 (MQ=255)
                                                                                          gggCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGa                                                             <  2:1295812/150‑1 (MQ=255)
                                                                                                gggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGt                                                       <  1:9773261/150‑1 (MQ=255)
                                                                                                  gTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAAACCCCACCCCCAAGGGGCGAGGGGGCGGTCGGGGCCCGTTTGTGCTGTGTCATCTGACGAAAAACGCGCCCAAGCGCCGGTAGAAAAAATTCGAAACAGTgg                                                     >  1:7406577/1‑149 (MQ=255)
                                                                                                        gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                               <  2:6379683/150‑1 (MQ=255)
                                                                                                        gTAAATAGTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                               <  1:2588885/150‑1 (MQ=255)
                                                                                                         tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                                              <  1:1010163/150‑1 (MQ=255)
                                                                                                         tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACAGTCTGAGCACTTTTGTACTTTTTCATCTGACTAAAAATGCGCCGAAGCGCCGTTAGAAAATAGTCGAATCAGTTGATTACTg                                              >  2:8390652/1‑150 (MQ=38)
                                                                                                                tCGCGCGAGTTGCGGCCTGTTCCCCTCAGCCTAAGCCTCTCCCCATAGGGGCGAGGGGACTGTCTGAGGACTTTTGTAGTTTGTCATCTGACTAACAAGGCGCCGTAGCGCCTTTTGAAATTCGTCGACTCAGTGAATTACTGGTATTCg                                       <  2:1427276/150‑1 (MQ=255)
                                                                                                                tCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGATTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCg                                       <  1:4382792/150‑1 (MQ=255)
                                                                                                                 cgcgCCAGTGCCGGCCTGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGc                                      <  2:6934265/150‑1 (MQ=255)
                                                                                                                 cgcgCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGc                                      <  1:7032401/150‑1 (MQ=255)
                                                                                                                  gcgcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAACTAGTCGAATCAGTGAATTACTGGTCTTCGCt                                     >  2:7577343/1‑150 (MQ=255)
                                                                                                                    gcCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTaa                                   <  1:2077328/150‑1 (MQ=255)
                                                                                                                       aGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCg                                <  2:4242422/150‑1 (MQ=255)
                                                                                                                       aGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATATGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCg                                <  2:715957/150‑1 (MQ=255)
                                                                                                                         tGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGt                              <  2:9306682/150‑1 (MQ=255)
                                                                                                                                 ggggccccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCCTCGGACTAAAAAGGCGCCGAAGCGCCTTTAGAACATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGa                      <  2:2854217/146‑1 (MQ=255)
                                                                                                                                   ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTa                                                                                                               >  2:5317494/1‑59 (MQ=255)
                                                                                                                                   ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTa                                                                                                               <  1:5317494/59‑1 (MQ=255)
                                                                                                                                   ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGc                    >  2:5946080/1‑150 (MQ=255)
                                                                                                                                      cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGa                 <  2:2208537/150‑1 (MQ=255)
                                                                                                                                      cccTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCGTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGa                 >  1:7737169/1‑150 (MQ=255)
                                                                                                                                       ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGaa                <  1:1780102/150‑1 (MQ=255)
                                                                                                                                        cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAAc               >  2:5057557/1‑150 (MQ=255)
                                                                                                                                            cccTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGt           >  1:9630865/1‑150 (MQ=255)
                                                                                                                                            acagtccccTCGCCCCTTTGGGGAGAGGGTACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGt           >  2:6275302/7‑150 (MQ=255)
                                                                                                                                             ccTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAATGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGTACAGGtt          >  1:930908/1‑150 (MQ=255)
                                                                                                                                               tAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTAc        >  2:1949489/1‑150 (MQ=255)
                                                                                                                                                aaCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACg       <  2:6243797/150‑1 (MQ=255)
                                                                                                                                                aaCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACg       <  2:3638684/150‑1 (MQ=255)
                                                                                                                                                aaCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACg       <  2:8883267/150‑1 (MQ=255)
                                                                                                                                                  cccTCGCCCCTTTGGGGAGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGt     >  2:1714172/1‑150 (MQ=255)
                                                                                                                                                   ccTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTc    <  1:5484331/150‑1 (MQ=255)
                                                                                                                                                     tctcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGc  <  2:6104845/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGC  >  NC_000913/3045919‑3046217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: