Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,611 C→A 5.1% intergenic (+66/‑333) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6110CA 5.1% 1398.8 / 47.0 426intergenic (+66/‑333)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base C (239/163);  new base A (4/18);  total (243/183)
Fisher's exact test for biased strand distribution p-value = 2.03e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-01

Only 100 of 433 total aligned reads displayed.

GGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTT  >  NC_000913/3239462‑3239760
                                                                                                                                                        |                                                                                                                                                     
ggCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCGGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTcc                                                                                                                                                       >  1:10164867/1‑150 (MQ=255)
 gCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCa                                                                                                                                                      <  2:1367606/150‑1 (MQ=255)
   gtggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAgg                                                                                                                                                    <  1:663769/150‑1 (MQ=255)
    tggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCACTCTCCCTTCCAggg                                                                                                                                                   >  2:5439503/1‑150 (MQ=255)
     ggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                                                                                  <  1:3523810/150‑1 (MQ=255)
       tgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                                <  2:3883142/150‑1 (MQ=255)
         tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCACCCTCTCCCTGGAAGGGAGAgg                                                                                                                                              <  2:5862479/150‑1 (MQ=255)
          ttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAggg                                                                                                                                             <  1:3343589/150‑1 (MQ=255)
           tGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGt                                                                                                                                            >  2:3606264/1‑150 (MQ=255)
           tGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGGTTTCTCCCTCTCCCTTCCAGGGAGAGGGt                                                                                                                                            >  2:9236192/1‑150 (MQ=255)
            ggCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTGACATGAGCACCGTTTTCACCCTCTCCCTGGAAGGGAGAgggag                                                                                                                                           <  2:2030755/150‑3 (MQ=255)
             gCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAggga                                                                                                                                            >  1:4531185/1‑147 (MQ=255)
             gCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAggga                                                                                                                                            <  2:4531185/148‑2 (MQ=255)
              cagtCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                                                                                         >  1:2574928/4‑150 (MQ=255)
               aTTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCggg                                                                                                                                        <  2:1245532/150‑1 (MQ=255)
               aTTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCggg                                                                                                                                        <  2:3449388/150‑1 (MQ=255)
               aTTCTGGTGATGACGTGTCTTATCACCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCCCCGTTGTCTCCCTCTCCCTTCCAGGGAGAGGGTCggg                                                                                                                                        <  2:6952790/150‑1 (MQ=255)
                ttCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCACTCCCTTCCAGGGAGAGGGTCgggg                                                                                                                                       >  2:6064660/1‑150 (MQ=255)
                 tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGt                                                                                                                                      <  2:5624669/150‑1 (MQ=255)
                   tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                    <  2:2892257/150‑1 (MQ=255)
                     gTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                                                                                                  <  2:7756967/150‑1 (MQ=255)
                        atgaCGTTTATTATCAACGCCTGGGTGAATTAACGGCATGATAAGCAGCGGGGGGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTACCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTa                                                                                                                               >  1:1591249/1‑150 (MQ=255)
                         tgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTaa                                                                                                                              >  1:5197585/1‑150 (MQ=255)
                          gaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAt                                                                                                                             >  2:10003512/1‑150 (MQ=255)
                              tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                         >  2:4428686/1‑150 (MQ=255)
                              tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                         >  1:2894405/1‑150 (MQ=255)
                              tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                         >  1:6828904/1‑150 (MQ=255)
                                tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCg                                                                                                                       >  1:5365113/1‑150 (MQ=255)
                                    atCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAcc                                                                                                                   >  1:4642509/1‑150 (MQ=255)
                                     tCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCg                                                                                                                  >  1:2150907/1‑150 (MQ=255)
                                           ccTGGGTTAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTg                                                                                                            <  2:3356889/150‑1 (MQ=255)
                                             ggggTTAATTCTGGGCTTTATATGCTGGGGGGTGTTTAATTTTTAATCTGCCTAAGCGTTTTACCCTGTCTTTAACTTTACCCCGGTTTCCCCCCTCTCCCTCCCTGGGTTTGGGTGGGGTTGTGGGTAATTTTTCGCACCGATGCTGGc                                                                                                          <  2:8791133/149‑1 (MQ=255)
                                              gggTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGcc                                                                                                         >  1:3932496/1‑150 (MQ=255)
                                               ggTGAATTATCGGCATTATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCGTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                                                                                        >  2:9516400/1‑150 (MQ=255)
                                                  gAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                    <  1:9416972/150‑1 (MQ=255)
                                                   ttttATGGGCATGATAAGCTGCGGGGTGGATAATTTTTATTCTGCCTATGCCGTGTACCCTGTCTTTAACATGCGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                    <  2:7551753/148‑1 (MQ=255)
                                                   aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                    >  1:1408080/1‑150 (MQ=255)
                                                      tATCGGCATTATAAGCAGCGGGGTGGATATTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                                 <  2:5067602/150‑1 (MQ=255)
                                                       aTCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCGGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGTGAGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                                <  2:6783064/150‑1 (MQ=255)
                                                           gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                            >  1:4903668/1‑150 (MQ=255)
                                                           gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                            >  1:2513736/1‑150 (MQ=255)
                                                           gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                            >  1:587247/1‑150 (MQ=255)
                                                               gATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTaa                                                                                        >  1:9691956/1‑150 (MQ=255)
                                                                aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAc                                                                                       >  1:1021911/1‑150 (MQ=255)
                                                                 tatgcaggGGGGTTTATAATTTTAATCGTGCCTCAGCGTGTTACCTTGTCATTAAATGGAGCAGCTTTTTCACCCCCTCCCTTCCTGGGTGAGGGTGGGGGTTAGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                                                                                      <  2:4024422/147‑1 (MQ=16)
                                                                 tAAGCTGCGGTTTGTATTATTTTTAATCTGCCTAAGCCTTGTACCCTGTCATTAACATGACCACCTTTTTCTCCATCTCCGTTCCCGGGAGTGGGTGGTGGTGTTGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                                                                                      <  2:6397258/150‑1 (MQ=255)
                                                                  aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                     >  2:1560604/1‑150 (MQ=255)
                                                                   tgccgggggtTGTATATTTTTTAATGTGCCTAGGCGTTTTACCGTTTATTTAAATTGCGCCCGTTTTTCCCCCTCGCCCTCCCCGGGTGTGGGTGGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCct                                                                                    <  2:2989342/144‑1 (MQ=9)
                                                                    gcagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAACCctc                                                                                   >  1:7914084/1‑150 (MQ=255)
                                                                      gggggggTGTTTATTTTTTATTCTGCCAACGCGGTGTACCCTGTCTTTAACATGTGCACGGTTTTGCCCCTGGCCCTGCCTGGGAGTGGGTGGGGGTGGGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCtctc                                                                                 <  2:8276220/147‑1 (MQ=16)
                                                                               gATTATTTTTAATCTGCCTAAGCGGTGTACCCTGTCATTAACCTGAGCCCCGTTTTCTCCCTCTCCCGACCAGGTATAGGGTGGGGGTGCGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                        <  2:2268874/150‑1 (MQ=37)
                                                                               gATATTTTTTAATCTGCCTAAGCGGTGTACCGTGTCTTTAACATGAGCACGGTTTTCACCCTCTCCCTCCCTGGGTGAGGGTCGGGGTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                        <  2:9352396/150‑1 (MQ=25)
                                                                                 tattttttAATCTCCCATCGCGGTGAACCTTTTATTTAAATTGCGCACTTTTTTCCCCCCCGCCCTCCCGGGGTGTGGGTGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                      <  1:8235794/147‑1 (MQ=12)
                                                                                 tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                      >  1:6426638/1‑150 (MQ=255)
                                                                                 ataTTTTTTATCGTGCCTACGCGGTGGACCGTGTATTTAACATGCGCACGTTTTTCGCCCCCGCCCTGCCGGTGTGTGGGTGGGGTTGGGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACggg                                                                       <  2:4786791/148‑1 (MQ=12)
                                                                                    tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                   >  1:8156050/1‑150 (MQ=255)
                                                                                     ttttAATGTGCCTATGCCGTGTCCCCTGTCTTTACATTGCGCACCGTTGTCACCCTCGCCCTCCCCGGGTGGGGGTGGGGGTGAGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                                  <  2:704044/150‑1 (MQ=21)
                                                                                      tttatgcTGCATAAGCCTGTTACCCTGAATTAAAATTGCGCACGTTTTTCTCCCTCGCCCTCCGGTGGTGTGGGGGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                                 <  2:178896/144‑1 (MQ=12)
                                                                                      tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                                 <  2:6617625/150‑1 (MQ=255)
                                                                                       ttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGTATTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAggg                                                                <  1:9343647/150‑1 (MQ=255)
                                                                                         aaTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCGCTCCCCAAACGGGGCGAGGGGa                                                              >  1:4419729/1‑150 (MQ=255)
                                                                                                ccTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                        >  2:9190129/1‑150 (MQ=255)
                                                                                                  tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                      >  2:3202696/1‑150 (MQ=255)
                                                                                                    aGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGt                                                    >  1:10052825/1‑150 (MQ=255)
                                                                                                             ccccgtgcATTAACAGGCGCCCCGTTTCCCCCCTCCCCCTCCCCGGTGGCGGGTGGGGGTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                            <  2:9738250/143‑1 (MQ=21)
                                                                                                             ccccTGACATTAACATGAGCACCGTGTTCTCCCACTCCCTCCCCGGGTTTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                            <  2:6245536/149‑1 (MQ=25)
                                                                                                               cttgacATCAACATGAGCACCGTTTGCCCCCTCCCCCTCCCCGGTAGTGGGTGGGGGTGCGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                          <  1:8963688/145‑1 (MQ=25)
                                                                                                               ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                          >  1:54645/1‑150 (MQ=255)
                                                                                                               ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                          <  2:10292751/150‑1 (MQ=255)
                                                                                                               ccTGTCATTAACATGAGCACCGTTTTCGCCCTCCCCCTCCCCGGGAGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                          <  1:6245063/150‑1 (MQ=25)
                                                                                                               ccTGACTTTAACTCGAGAACCTTTTTCACCCTCTCCTTCCCCGGGTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                          <  1:6010113/150‑1 (MQ=21)
                                                                                                                 tGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtg                                        >  1:6892387/1‑150 (MQ=255)
                                                                                                                    cATTAACTTGAGCCCGGTTTTCGCCCGCTCCCTTCCCGGTTGCGGGTGGGGGTGTGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttg                                     <  2:9049585/150‑1 (MQ=25)
                                                                                                                    aaTTAACCTGTGCACCTTTTTCTCCCTCCCCTTTCCGGGGTTTGGGGGGGGGTTTGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttg                                     <  1:7357400/149‑1 (MQ=25)
                                                                                                                        aaaaTGTGCACGTTTTTCTCCTTGTCCCTTCCTGGTTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcat                                 <  1:10004975/147‑1 (MQ=25)
                                                                                                                         aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatc                                >  1:1092624/1‑150 (MQ=255)
                                                                                                                         aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAGGTTGtcatc                                >  1:7654625/1‑150 (MQ=255)
                                                                                                                           aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                              >  2:7265408/1‑150 (MQ=255)
                                                                                                                              gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTc                            >  2:10196643/1‑150 (MQ=255)
                                                                                                                               aGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCt                           <  2:2592901/150‑1 (MQ=255)
                                                                                                                                 cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                         >  2:8732515/1‑150 (MQ=255)
                                                                                                                                 cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                         >  2:7488614/1‑150 (MQ=255)
                                                                                                                                 cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCATTTTTTATGTTGTCATCAGTCTgg                         >  1:6518056/1‑150 (MQ=255)
                                                                                                                                   ccGTGTTCCCCCTCGCCCTCCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGaa                       <  2:8410110/150‑1 (MQ=25)
                                                                                                                                     gTTTTCTCCCTACCCCTCCCCGGGTTTGGTTCGGGTTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGc                     <  1:8347924/150‑1 (MQ=25)
                                                                                                                                     gTTTTCCCCCGCCCCCTCCCGGGGTGTGGGGGGGGGTTTGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGc                     <  2:3763316/150‑1 (MQ=25)
                                                                                                                                      ttttCTCCCTCTCCCTTCATGGTTGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGcc                    <  2:4087108/150‑1 (MQ=37)
                                                                                                                                       tttCCCCCCCCCCCTTCCTGGGTGTGGGTGGGGGGTTGGGCAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCg                   <  1:3620884/150‑1 (MQ=25)
                                                                                                                                       tttCCCCCCCCCCCTCCCTGGGTGGGGGTGGGGGTGCGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCg                   <  1:8751641/150‑1 (MQ=25)
                                                                                                                                         tctccctctcccTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGACGCa                 >  1:716575/1‑150 (MQ=255)
                                                                                                                                          ctccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGAAAATTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAACCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCAGGAAGCCGCAc                >  2:7992528/1‑150 (MQ=255)
                                                                                                                                            ccctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCTACCTAAACCTCTACCCAAACGGGGCGAGGGGACTTACCGAGTGCTTTTTTGATGTTTTATTAAGTCTGGAATACGCACGt              >  1:9099127/1‑150 (MQ=255)
                                                                                                                                             cctctccctTCCTGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt             <  2:10269280/150‑1 (MQ=255)
                                                                                                                                              ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                                                                                      <  1:1839563/76‑1 (MQ=255)
                                                                                                                                              ctctccctGCCCGGGAGTGGGGGGGGGTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg            <  2:5299391/150‑1 (MQ=37)
                                                                                                                                                 cccctCCCCGGGTGTGGGGGGGGGGGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt         <  1:9897714/149‑1 (MQ=37)
                                                                                                                                                   cctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCttt       >  2:5121876/1‑150 (MQ=255)
                                                                                                                                                    ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTa      >  1:4826829/1‑150 (MQ=255)
                                                                                                                                                     ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt     >  2:765614/1‑150 (MQ=255)
                                                                                                                                                     ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCACCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt     >  1:2303817/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
GGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTT  >  NC_000913/3239462‑3239760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: