Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,391,407 A→C 17.6% F208V (TTT→GTT)  tldD ← putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,391,4070AC17.6% 1915.8 / 146.9 820F208V (TTT→GTT) tldDputative peptidase
Reads supporting (aligned to +/- strand):  ref base A (433/224);  new base C (2/161);  total (435/385)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 846 total aligned reads displayed.

CGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGC  >  NC_000913/3391258‑3391556
                                                                                                                                                     |                                                                                                                                                     
 ggCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCaaaa                                                                                                                                                      >  1:8277546/1‑150 (MQ=255)
  gCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAAc                                                                                                                                                     <  2:1006877/150‑1 (MQ=255)
  gCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAAc                                                                                                                                                     <  2:8137876/150‑1 (MQ=255)
  gCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAAc                                                                                                                                                     <  2:3264376/150‑1 (MQ=255)
     aaCCTGCGCCACGTACGACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCTGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAc                                                                                                                                                  <  2:10196486/150‑1 (MQ=255)
        cTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAccgc                                                                                                                                               >  1:4627797/1‑150 (MQ=255)
        cTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGAccgc                                                                                                                                               >  2:3147551/1‑150 (MQ=255)
                  tactacCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACt                                                                                                                                     <  2:260375/150‑1 (MQ=255)
                     tacCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGc                                                                                                                                  >  2:4912067/1‑150 (MQ=255)
                      acCGGCTTGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGAAAGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTggta                                                                                                                                 <  2:538035/150‑3 (MQ=255)
                                tGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcgttcgc                                                                                                                       >  2:3974839/1‑150 (MQ=255)
                                   ccGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACcgcgttcgcgtt                                                                                                                    <  2:6237254/150‑1 (MQ=255)
                                    cGCTGGTGCAGCAACGGCTTAACTATTTACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATGGGAAAGTATGAATTAATACCCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGttt                                                                                                                   <  2:3842472/150‑1 (MQ=255)
                                     gCTGGTGCAGCAACGGCAGAAAGATTGCCCCGCGCCACACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTAATACCCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTg                                                                                                                  <  2:46888/150‑1 (MQ=255)
                                      cTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGAACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGc                                                                                                                 <  2:9159961/150‑1 (MQ=255)
                                       tGGTGCAGCAACGGCAGAAAGATGGACCCGCGCCATCCGCACTGCTTCTTTTGCCCAGGCATCCGCACGGACTTCGCCGTCGAGATCGGCACGGAAGAATTCATACCCACAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGcc                                                                                                                <  2:9385009/150‑1 (MQ=255)
                                          tGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtc                                                                                                             <  2:8564852/150‑1 (MQ=255)
                                          tGCAGCAACGGAAGAAAGTTTGCCCTGCGCCATCCGCACTGCTTCTTTTGCCCTGGCATCCGCAGGGACTTCGCCGTCGAGATCGGCATGTAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtc                                                                                                             <  2:290871/150‑1 (MQ=255)
                                            cagcaACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtctt                                                                                                           >  1:10209118/1‑150 (MQ=255)
                                            cagcaACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtctt                                                                                                           <  2:5200646/150‑1 (MQ=255)
                                            cagcaACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtctt                                                                                                           >  1:5639733/1‑150 (MQ=255)
                                            cagcaACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtctt                                                                                                           <  2:7829950/150‑1 (MQ=255)
                                             agcaACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGAGCGGCAAGGAAGAATTCAAAACCAAAACGACCGCAGCCGCCACTGGCACCGCGTTCGCGTTTGCCAtcttc                                                                                                          >  2:245352/1‑150 (MQ=255)
                                             agcaACCGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCCAGATCGGCAAGGAAGAATTCCTAACCAACACGACCGCCGCCGCCACAGGCACCGCGATCGCGATTGcccacatc                                                                                                          >  2:3732386/1‑144 (MQ=255)
                                               caACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATcttctt                                                                                                        >  1:8538728/1‑150 (MQ=255)
                                                  cGGCAGAAAGATTGACCAGCGCCATCCGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcga                                                                                                     <  2:1711164/150‑1 (MQ=255)
                                                  cGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGCCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcga                                                                                                     <  2:7228470/150‑1 (MQ=255)
                                                   ggCATAAAGATTGACCAGCGCCATCCGCACTGCTTCTTTGGCCCATCCATCCGCACGCACTTCGCCGTCTTGATCGCAACGGAATAATACATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcgac                                                                                                    <  1:5285045/150‑1 (MQ=255)
                                                   gccataaaGATTGCCCCGCGCCAACCGCACTTCTTCTTTTGCCCCTGCACCCGCACGGACTGCGCGGTCTATATCGGAATGGAAGAATTCAAACCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTcgac                                                                                                    <  1:9346943/145‑1 (MQ=255)
                                                    gcagcccgATTGCCCAGCGCCAGCCGCACGGCTTCTTGGGCCCCTGCATCCGCCCGGCCTGCGCCGCCCAGCTCGGCAAGGCAGCCATCATACCCACCCCGACCGCCGCCGCCACGGGCACCGCGTTCGCGTTTGCCATCTTCTTcgacg                                                                                                   <  2:7019900/143‑1 (MQ=255)
                                                      aGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgag                                                                                                 >  2:7667829/1‑150 (MQ=255)
                                                      aGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgag                                                                                                 >  1:98209/1‑150 (MQ=255)
                                                       taaaGTTTGACCAGCGCCATACGCACTGGTTATTTGGCCCATGCATCCGCACGGACTTCGCCGTCGAGAGCGGCAAGGAATAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACgaga                                                                                                <  2:9872845/149‑1 (MQ=255)
                                                        aaaGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGaa                                                                                               >  2:4280817/1‑150 (MQ=255)
                                                          aGATTGCCCCGCGCCATCCGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACg                                                                                             <  2:1135838/150‑1 (MQ=255)
                                                             ttGACCCGCGCCATACGCACTGTTTTTTTTGCCCTTGAATCCGCACGGACTTCGCCTTCTAGATCGGCAAGTAATAATTAAAAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTc                                                                                          <  1:2487144/150‑1 (MQ=255)
                                                              tGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCa                                                                                         >  1:2887655/1‑150 (MQ=255)
                                                               gACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCAc                                                                                        <  2:8277546/150‑1 (MQ=255)
                                                                aCCAGCGCCATCCGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTGCGCCGTCGTGTTCGGAAAGGAATAATTAATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACg                                                                                       <  1:1627850/150‑1 (MQ=255)
                                                                aCCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACg                                                                                       >  2:1417061/1‑150 (MQ=255)
                                                                aCCAGCGCCATACACACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACg                                                                                       >  2:8810035/1‑150 (MQ=255)
                                                                 ccAGCGCCATCCTCATTTTTTATTTTGCCCATGAATCCGCACTGTCTTCGCCGTCTATTTGGGAAAGTAATATTTAATAACAAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACgg                                                                                      <  1:3818692/150‑1 (MQ=255)
                                                                   aGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGaa                                                                                    >  2:9566628/1‑150 (MQ=255)
                                                                       ccttACTCACTGCTTCTTTTGCCCTGGCATCCGCACGTACTGGGCCGTCGAGATCGGAATGTAATAATACATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGAc                                                                                <  1:9036256/147‑1 (MQ=255)
                                                                        cATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGAGCGCCAAGGAATAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACg                                                                               <  1:5011076/150‑1 (MQ=255)
                                                                         aTACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGc                                                                              >  1:5741321/1‑150 (MQ=255)
                                                                          tccgaaCTGCTTATTTTGCCCAGGCAACCGCAGGTAGTGCGCCGTCGAGATCGGCACGGAATAATCCAAAACCCACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCa                                                                             <  1:7076630/145‑1 (MQ=255)
                                                                               aCTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGc                                                                        >  1:5925292/1‑150 (MQ=255)
                                                                                cTGCTTCTTTGGACCATGCATCCGCACGTCTTTCGCGGTCTTTTTCGGCATGGATTATTTCATACCAAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcg                                                                       <  1:6059060/150‑1 (MQ=255)
                                                                                  gCTTCTTTTGCCCATGCATCCGCACGTACTTCGCCGTCGATATCGGCAAGGAAGAATTAATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgga                                                                     <  1:8375456/150‑1 (MQ=255)
                                                                                  gCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcgga                                                                     >  1:6556713/1‑150 (MQ=255)
                                                                                   tttcttTGGCCCATGCATCCGCACGTACTTCGCCGTCTAGATCGGAAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggac                                                                    <  1:2275905/149‑1 (MQ=255)
                                                                                    tccttTTGCCCATGCATCCGCACGGACTTCGCCGTCTAGATCGGCAAGTAAGAATACATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggacg                                                                   <  2:1918629/148‑1 (MQ=255)
                                                                                     tcttTTGCCCATGCATCCGCACGGACTTCGCCGTCTAGATCGGAAAGGAAGAATACATAACAAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGcggacgg                                                                  <  1:8839966/150‑1 (MQ=255)
                                                                                       ttttGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCggacggac                                                                >  1:3226877/1‑150 (MQ=255)
                                                                                       ttttGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCggacggac                                                                <  2:4051224/150‑1 (MQ=255)
                                                                                       ttgtccccATGCATCCGCACGGACTTCGCCGTCGAGATGGGCAAGGAAGAATACAAAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCggacggac                                                                <  1:1001635/145‑1 (MQ=255)
                                                                                            cccATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccg                                                           >  2:3565430/1‑150 (MQ=255)
                                                                                            cccATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccg                                                           >  2:4449580/1‑150 (MQ=255)
                                                                                             ccATGCATCCGCACGGACTTCGCCGTCTAGATCGGCACGGAAGAATTAATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgc                                                          <  1:9431976/150‑1 (MQ=255)
                                                                                             ccATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgc                                                          >  2:6654280/1‑150 (MQ=255)
                                                                                             ccATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgc                                                          >  1:1174020/1‑150 (MQ=255)
                                                                                             ccATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgc                                                          >  1:3576941/1‑150 (MQ=255)
                                                                                             ccATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgc                                                          >  2:7401070/1‑150 (MQ=255)
                                                                                             ccATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATccgc                                                          >  1:683671/1‑150 (MQ=255)
                                                                                                   aTCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAg                                                    >  1:1541386/1‑150 (MQ=255)
                                                                                                    tCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCAAAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGc                                                   <  1:1962375/150‑1 (MQ=255)
                                                                                                       gCACGGACTTCGCCGTCGATATCGGCAAGGAAGAATTAATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTg                                                <  1:3316207/150‑1 (MQ=255)
                                                                                                         aCGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGcc                                              <  1:5376755/150‑1 (MQ=255)
                                                                                                         aCGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGcc                                              <  2:8390148/150‑1 (MQ=255)
                                                                                                           ggACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGt                                            >  1:1082418/1‑150 (MQ=255)
                                                                                                             aCTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCg                                          <  2:6782951/150‑1 (MQ=255)
                                                                                                               ttCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGt                                        >  2:8654347/1‑150 (MQ=255)
                                                                                                                 cGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTgg                                      <  1:412434/150‑1 (MQ=255)
                                                                                                                   ccGTCGATATCGGCAAGGAAGAATTCATACCCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGcc                                    <  1:3001850/150‑1 (MQ=255)
                                                                                                                   ccGTCGAGATCGGCAAGGAATAATTCATACCCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGcc                                    <  1:9670701/150‑1 (MQ=255)
                                                                                                                   ccGGCGAGAGCGGCAAGGAAGAATCCATCACCCAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGcc                                    <  2:2116853/150‑1 (MQ=255)
                                                                                                                      tCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCa                                 >  2:7960285/1‑150 (MQ=255)
                                                                                                                        gagaGCGGCAAGGAAGAATACATAACCAAAACGACCCCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAc                               <  2:5739664/150‑1 (MQ=255)
                                                                                                                         agaTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAcc                              <  2:5531835/150‑1 (MQ=255)
                                                                                                                           aTCGGCAAGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCTACCaa                            <  2:6108258/150‑1 (MQ=255)
                                                                                                                            tCGGCAAGGAAGAATTCATAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCaaa                           <  2:6435645/150‑1 (MQ=255)
                                                                                                                             cGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCaaaa                          <  1:3923598/150‑1 (MQ=255)
                                                                                                                              ggCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGTCGTCGGTGGCCGCAACCAAaat                         >  2:2242557/1‑150 (MQ=255)
                                                                                                                              ggCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaat                         >  2:9715764/1‑150 (MQ=255)
                                                                                                                                cAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaatta                       >  1:6257282/1‑150 (MQ=255)
                                                                                                                                cAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaatta                       >  1:126202/1‑150 (MQ=255)
                                                                                                                                 atggATGATTTCATAACCACCACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaa                      <  1:8021248/148‑1 (MQ=255)
                                                                                                                                 aaGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAaattaa                      <  2:2055970/150‑1 (MQ=255)
                                                                                                                                  aGGAAGAATTCATAACCACAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAtaattaat                     <  1:7826459/150‑1 (MQ=255)
                                                                                                                                    gaagaaTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTc                   >  1:5216681/1‑150 (MQ=255)
                                                                                                                                     aagaaTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCa                  >  2:5844241/1‑150 (MQ=255)
                                                                                                                                      agatTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCAt                 <  1:179560/150‑1 (MQ=255)
                                                                                                                                       gaaTTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATa                <  2:9262339/150‑1 (MQ=255)
                                                                                                                                          ttaaTAACCAACACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGac             <  1:2651548/147‑1 (MQ=255)
                                                                                                                                          ttCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGac             <  2:5621957/150‑1 (MQ=255)
                                                                                                                                              tAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGAcacca         <  1:4401156/150‑1 (MQ=255)
                                                                                                                                                 ccAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTg      >  2:600154/1‑150 (MQ=255)
                                                                                                                                                  cAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGa     >  1:7401268/1‑150 (MQ=255)
                                                                                                                                                   aaaaCGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAg    <  2:1550647/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGC  >  NC_000913/3391258‑3391556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑