Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,695 C→G 9.8% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,6950CG 9.8% 1826.8 / 45.2 581intergenic (+46/+56)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (332/181);  new base G (3/64);  total (335/246)
Fisher's exact test for biased strand distribution p-value = 6.49e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 620 total aligned reads displayed.

CCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATA  >  NC_000913/3608546‑3608844
                                                                                                                                                     |                                                                                                                                                     
ccacGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCACGCCCctc                                                                                                                                                       >  1:6490401/1‑150 (MQ=255)
 ctcGCTGTTAGGGATGACCGGGTTGTGGCTGGCTGTGCTGGCATATAGGGGGGCGACGGTGCTGGTGACAGCGATTGCTTTAAGATTGTTGCGCTGGATATAAGGCAAACCGATCGAAACTTTGAGCGCGATCGGTCCCCTCGCCCctct                                                                                                                                                      <  1:4609783/148‑1 (MQ=255)
 cacGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCctct                                                                                                                                                      >  2:9177997/1‑150 (MQ=255)
 cacGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCctct                                                                                                                                                      >  1:4778859/1‑150 (MQ=255)
  acGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTg                                                                                                                                                     >  2:7892712/1‑150 (MQ=255)
  acGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTg                                                                                                                                                     <  2:7213473/150‑1 (MQ=255)
  acGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTg                                                                                                                                                     >  1:9804026/1‑150 (MQ=255)
    gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                   <  2:8858990/150‑1 (MQ=255)
    gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                   <  1:6907865/150‑1 (MQ=255)
      tGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                 <  2:5552775/150‑1 (MQ=255)
        ttAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCATCATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGtaga                                                                                                                                               <  2:608270/150‑1 (MQ=255)
        ttAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                                                                               >  1:8644485/1‑150 (MQ=255)
        tgaGGGCTGACCGGGGTGTGGCTGGCAGTGCTGGCAGATTCGGGGGCGACGGTGCGGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGCGCGCGATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                                                                               <  1:7491194/148‑1 (MQ=255)
          aGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCTCCCCAGAGGGGCGAgg                                                                                                                                             <  2:8773080/150‑1 (MQ=255)
          aGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAgg                                                                                                                                             <  2:6432807/150‑1 (MQ=255)
           gggAGGACCGGGTTGTGGCTGGCAGTGCGGGCAGATCCGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                                                                                            <  2:3273213/150‑1 (MQ=255)
              aTGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                                                                                         >  2:5589214/1‑150 (MQ=255)
               tGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                           <  1:7029732/147‑1 (MQ=255)
               tGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAg                                                                                                                                        >  2:1153372/1‑150 (MQ=255)
                gACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                                       <  2:1244091/150‑1 (MQ=255)
                gACCGGGGTGGGGCTGGCAGTGCTGGCAGATACGGGGGCGCCGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGACCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTGAgg                                                                                                                                       <  2:3414095/150‑1 (MQ=255)
                  ccGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGt                                                                                                                                     <  1:7350470/150‑1 (MQ=255)
                   cGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTg                                                                                                                                    >  1:2021126/1‑150 (MQ=255)
                     ggTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAg                                                                                                                                  <  1:5365145/150‑1 (MQ=255)
                      gTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                 <  2:9489810/150‑1 (MQ=255)
                               ggcAGTGCTGGCCGATACGGGGGCGACGGTGGTGGTGACTGCGAATGCTTTTAGATTGTTGCGCAGGAGATAAGGCTACCCTCTCGCAACTTTGAGCGCGCTCGGTCCCCTCGCCCCTCTGGGGAGAGGTTTAGGGTGAGGGGAAAAggc                                                                                                                        <  2:9871071/150‑1 (MQ=37)
                               ggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGTGTGAGGGGAAAAggc                                                                                                                        <  2:6824194/150‑1 (MQ=255)
                                gctgTGCTGGCAGATCCGGGGGCGACGGTGCTGTTGACAGCGAATGCTTTATGTTTGTTGCGCAGGAGATAAGGCAACCCGATCGAAACATTGCGCGCTATCGGTCCCCTCGCCCCTCGGGGTAGAGGGTTTGGGTGTGGGGAAAAggcg                                                                                                                       <  2:7792158/147‑1 (MQ=255)
                                gccgTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGATTGCGTTAAGATTGTTGGGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcg                                                                                                                       <  2:8706377/147‑1 (MQ=255)
                                gcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcg                                                                                                                       >  1:3508469/1‑150 (MQ=255)
                                gcAGTGCAGGCAGATACGGCGGCGACGGTGCTGGTGACAGCGAATGCGTGAAGATTGTTGCGCAGGAGAACCGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGCGTGAGGGTGAGGGGACAAggcg                                                                                                                       >  1:7811240/1‑150 (MQ=38)
                                 ctttGCTGGCAGATACGGGGGCGAGGGTGCTGGTTAATGCGATTGCTTTATGTTTTTTGCGCAGTATATATGGCAAACCGATCGCAACTTTGTGCGCTATGGGTCCCCTCGCCCATCTGGGTTGTGGTTTTGGTTGAGGGTAAAAggcgg                                                                                                                      <  2:5035516/147‑1 (MQ=255)
                                  aGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcggc                                                                                                                     >  1:1319629/1‑150 (MQ=255)
                                   gTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCa                                                                                                                    >  1:3340239/1‑150 (MQ=255)
                                       tGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGGGAGGGGAAAAGGCGGCATCGa                                                                                                                >  2:2572213/1‑150 (MQ=255)
                                                  ggggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGc                                                                                                     >  1:9176417/1‑150 (MQ=255)
                                                    ggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                                   >  2:6944174/1‑150 (MQ=255)
                                                     gCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGcccc                                                                                                  >  2:1905892/1‑150 (MQ=255)
                                                      cgcgggTGCTGGTGACAGCTATTGCGTTAATTTGGTGGGGCTGGAGATACGGCAAACCGATCGCAACATTGCGCGCGATGGGTCCCCTCGCCCCTCTGGGGTGTGGGTTTGGGTTTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCt                                                                                                 <  2:4137832/146‑1 (MQ=255)
                                                      cGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCt                                                                                                 >  1:245988/1‑150 (MQ=255)
                                                       gACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTa                                                                                                >  1:7652392/1‑150 (MQ=255)
                                                       gACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTa                                                                                                >  2:8988685/1‑150 (MQ=255)
                                                           gtggtggtgACAGCTAATGCTTTATGTTTGTTGCGCCGGATATACGGAAAACCTATCGAAAAATTGAGCGCGATGGGTCCCCTCGCCCCTCGGGGGAGTGGGTTTGGGTGCGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCaa                                                                                            <  2:8258147/150‑1 (MQ=255)
                                                               tGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGc                                                                                        >  1:2915665/1‑150 (MQ=255)
                                                                  tGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCtt                                                                                     >  2:2211603/1‑150 (MQ=255)
                                                                        cGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGaa                                                                               >  1:5683862/1‑150 (MQ=255)
                                                                         gAATGCTTTATGTTTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACTTTGAGCGCGATCGGTCCCCCCGCCCCTCTGGGGTGCGGTTTCGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAAt                                                                              <  2:7369313/150‑1 (MQ=255)
                                                                         gAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAAt                                                                              >  1:5181803/1‑150 (MQ=255)
                                                                          aaTGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATc                                                                             <  2:7739953/150‑1 (MQ=255)
                                                                             ggtttATGATTTTTGCGCTGGATATACGCAAACCCGACCCCAAAATTGCGCGCGATGGGTCCCCGCGCCACTGTGGGTTGTGGGTTTGGTTGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCaaa                                                                          <  1:371047/147‑1 (MQ=255)
                                                                             ggtttAAGTTTGTTGCGCAGGTTATACGGCAACCCGAGCGAAACATGGCGCGCTATCGGTCCCCTCGCCCCTCTGGGGATCGGGTTAGGGTGAGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCaaa                                                                          <  1:4201537/147‑1 (MQ=37)
                                                                               gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACAATGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                        >  2:9659546/1‑150 (MQ=255)
                                                                                ttttgtttgttgCGCTGTTGATAAGGAAAACCTTTCCCAAATTTGCGGGCGATGGGGCCCCTCGCCCCTGTGGTTTGTGGGTTTGGGTGTGGTGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                       <  2:3819787/144‑1 (MQ=255)
                                                                                ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                       >  1:9444157/1‑150 (MQ=255)
                                                                                     aTTGTGGCGCAGGAGAGAAGGCAACCCGAACGCAACTTGGCGCGCGAGCGGTCCCCTCGCCCCTGTGGGTTGAGGTTTCGGGTGGGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATa                                                                  <  1:4332174/150‑1 (MQ=255)
                                                                                      tttttgCGCTTGAGATACGCAAAACCGATCGAAACTTTGCGCGCGTTCGGCCCCCTCGCCCCTGGGGGGTGTGGGTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                 <  1:8423743/147‑1 (MQ=255)
                                                                                      ttgttgCGCTGGATATAAGCAAACCCGATCGAAACTTGGCGCGCGTTCGGTCCCCCCGCCCCGCGGGGGTGTGGGTTTGGGTGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                 <  1:1154718/150‑1 (MQ=255)
                                                                                            cgcAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATAACGATAAGGCAGt                                                           >  1:10031697/1‑150 (MQ=255)
                                                                                             gcAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTc                                                          >  1:6773211/1‑150 (MQ=255)
                                                                                                ggATATAAGCCAACCCGATCCAAACTTTGCGCGCTTGCGGTCCCCTCGCCCCTGTGGGGTTAGGTTTTGGGTTTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCAt                                                       <  1:1055718/150‑1 (MQ=255)
                                                                                                 gagaTAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATc                                                      >  1:8867630/1‑150 (MQ=255)
                                                                                                  ataTACGGCAACCCGATCGCAACTTTGCGCGCGTTCGGCCCCCCCGCCCCTCTGGGGAGCGGGTTCGGGTGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATcc                                                     <  2:4643313/148‑1 (MQ=255)
                                                                                                    aTAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGt                                                   >  1:3180178/1‑150 (MQ=255)
                                                                                                      aaGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtct                                                 >  1:8097808/1‑150 (MQ=255)
                                                                                                      aaGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtct                                                 >  1:9644872/1‑150 (MQ=255)
                                                                                                      aaGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtct                                                 >  2:9785936/1‑150 (MQ=255)
                                                                                                      aaGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtct                                                 >  1:832584/1‑150 (MQ=255)
                                                                                                       aGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtctc                                                >  2:1693676/1‑150 (MQ=255)
                                                                                                       aGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGGTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtctc                                                >  2:1476454/1‑150 (MQ=255)
                                                                                                            aaCCGATCGCAACATTGAGCGCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAg                                           >  2:7598588/1‑150 (MQ=255)
                                                                                                              cctaTCCCAACATGTAGCGCGATCGGACCCCTCGCCCCTCTGGGGTGTGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCa                                         <  1:613310/147‑1 (MQ=255)
                                                                                                                gATCGCAACATTGCGCGCGATGGGCCCCCCCGCCCCTCGGGGGTGTGGGTTTGGGGGGGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                       <  1:181524/150‑1 (MQ=255)
                                                                                                                gATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                       >  2:1016158/1‑150 (MQ=255)
                                                                                                                 aTCGCAACTTTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAcc                                      <  2:10045147/150‑1 (MQ=255)
                                                                                                                   cGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAg                                    >  1:8791658/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                  >  2:7681064/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                  >  2:397740/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                  >  1:5850410/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                  >  1:1616875/1‑150 (MQ=255)
                                                                                                                            gcgcgcGATCGGGCCCCTCGCCCGCGTGGGGAGTGGGTTCGGGTGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTc                           <  2:7062291/148‑1 (MQ=255)
                                                                                                                               cgcgATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCACCCAGTTCGTGTTCCAt                        >  1:4049216/1‑150 (MQ=255)
                                                                                                                               cgcgATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAt                        >  2:6634343/1‑150 (MQ=255)
                                                                                                                               cgcgATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAt                        >  2:938812/1‑150 (MQ=255)
                                                                                                                               cgcgATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAt                        <  2:10248117/150‑1 (MQ=255)
                                                                                                                                 cgATCGGTCCCCGCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaa                      <  2:2865225/150‑1 (MQ=255)
                                                                                                                                  gATCGGTCCCCTCGCCCCTCGGGGGAGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                     <  2:4886555/150‑1 (MQ=255)
                                                                                                                                     gggTCCCCTCGCCCCTCGGGGGTGTGGTTTTGGGTGTGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGt                  <  2:2710068/149‑1 (MQ=255)
                                                                                                                                      ggaccccccccccCTCGGGGGTTTTGGTTTGGGTGAGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTa                 <  1:2609534/147‑1 (MQ=255)
                                                                                                                                      ggTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTa                 >  1:800093/1‑150 (MQ=255)
                                                                                                                                        tCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATACGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACa               >  2:8119771/1‑150 (MQ=255)
                                                                                                                                           ccgcGCCCGTGTGGGTTGTGGGTTAGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaaa            <  2:3634658/147‑1 (MQ=255)
                                                                                                                                           ccTCGCCCCTGGGGGGTGTGGGTTTGGGTGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaaa            <  2:3016814/150‑1 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAc           >  1:2232212/1‑150 (MQ=255)
                                                                                                                                             tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAcc          >  2:5891071/1‑150 (MQ=255)
                                                                                                                                               gCCCCTCTGGGGTGTGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa        <  2:8460498/150‑1 (MQ=255)
                                                                                                                                               gCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa        <  1:7746853/150‑1 (MQ=255)
                                                                                                                                                 cccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAgg      >  1:7205696/1‑150 (MQ=255)
                                                                                                                                                   ctctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATACAGGTCCAAAACCCAGGaa    >  2:5292518/1‑150 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat   >  2:7487213/1‑150 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat   >  1:9254094/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATA  >  NC_000913/3608546‑3608844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: