Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,721 A→C 8.5% intergenic (+72/+30) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,7210AC 8.5% 1624.3 / 31.3 577intergenic (+72/+30)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (342/168);  new base C (3/63);  total (346/231)
Fisher's exact test for biased strand distribution p-value = 4.22e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 603 total aligned reads displayed.

TGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCGGC  >  NC_000913/3608572‑3608870
                                                                                                                                                      |                                                                                                                                                     
tggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaa                                                                                                                                                       >  1:8843564/1‑150 (MQ=255)
   ctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAg                                                                                                                                                    >  1:7698699/1‑150 (MQ=255)
    tggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                                                                   >  1:9891788/1‑150 (MQ=255)
      gccgTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGATTGCGTTAAGATTGTTGGGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcg                                                                                                                                                 <  2:8706377/147‑1 (MQ=255)
        aGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcggc                                                                                                                                               >  1:919337/1‑150 (MQ=255)
         gTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCa                                                                                                                                              >  1:3340239/1‑150 (MQ=255)
          tGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCAt                                                                                                                                             >  1:6646666/1‑150 (MQ=255)
               gCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAg                                                                                                                                        >  1:7378833/1‑150 (MQ=255)
               gCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAg                                                                                                                                        >  1:7640827/1‑150 (MQ=255)
                   aTACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCaa                                                                                                                                    >  2:721891/1‑150 (MQ=255)
                       gggggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGGTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAg                                                                                                                                >  2:9422397/1‑150 (MQ=255)
                          ggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                                                             >  2:6944174/1‑150 (MQ=255)
                          ggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                                                             >  1:5640028/1‑150 (MQ=255)
                            cGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCt                                                                                                                           >  1:245988/1‑150 (MQ=255)
                             gACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCCATCAGCCCCTa                                                                                                                          >  1:6412072/1‑150 (MQ=255)
                             gACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTa                                                                                                                          >  1:1439335/1‑150 (MQ=255)
                                  tggtGGTGACAGCGATTGCGTTTTGATTGTTGCGCTGGAGTTAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAAc                                                                                                                     <  2:6851203/147‑1 (MQ=38)
                                  tGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGACCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAATAGGCGGCATCGAAGCCAATCAGCCCCTATCAAc                                                                                                                     <  2:7648080/150‑1 (MQ=255)
                                     tGGTTACAGCTAATGCGTTATTATTGTTGCGCAGGATATATGGCAAACCGATCGCAACTTTGAGCGCGATGGGTCCCCTCGCCCCTGTGGGGAGAGGGTTTGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGc                                                                                                                  <  2:4869459/150‑1 (MQ=255)
                                     tGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCTCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGc                                                                                                                  >  1:4871436/1‑150 (MQ=255)
                                          aCAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                                             >  1:1944185/1‑150 (MQ=255)
                                          aCAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                                             >  2:8078299/1‑150 (MQ=255)
                                                   ggtttATGATTTTTGCGCTGGATATACGCAAACCCGACCCCAAAATTGCGCGCGATGGGTCCCCGCGCCACTGTGGGTTGTGGGTTTGGTTGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCaaa                                                                                                    <  1:371047/147‑1 (MQ=255)
                                                   gCGTTAAGATTTTGGCGCAGTATATACGGCAAACCGCTCGAAACATGGCGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCaaa                                                                                                    <  1:4201731/150‑1 (MQ=37)
                                                     gttacttttgttgCGCTGTAGATACGTCAACCCGATCGCAACATTGAGCGCGCTCGGTCCCCGCGCCCCTGTGGGGTGTGGTTTTGGTTGTGGGTACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                                                  <  1:1095936/143‑1 (MQ=255)
                                                     gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                                                  >  1:4393384/1‑150 (MQ=255)
                                                      ttaattttgttgCTCTTGAGATACGGCAACCCTCTCGAATATTGGCGCGCGATGGGGCCCCTCCCCCCTGTGGGGTGTGGGTTCGGGTGTGGTGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                                                 <  2:4627269/144‑1 (MQ=255)
                                                       tAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAAc                                                                                                >  1:462094/1‑150 (MQ=255)
                                                           aTTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATa                                                                                            >  2:10187236/1‑150 (MQ=255)
                                                           aTTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATAACGATa                                                                                            >  1:6883418/1‑150 (MQ=255)
                                                            tttttgCGCTTGAGATACGCAAAACCGATCGAAACTTTGCGCGCGTTCGGCCCCCTCGCCCCTGGGGGGTGTGGGTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                                           <  1:8423743/147‑1 (MQ=255)
                                                            ttgttgCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCACTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                                           <  1:7127434/150‑1 (MQ=255)
                                                                  cgcAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATAACGATAAGGCAGt                                                                                     >  1:10031697/1‑150 (MQ=255)
                                                                        agaTATGGCAAACCGTTCGAACCATTGAGCGCTATGGGTCCCCTCGCCCCTCTGGGTTGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATcc                                                                               <  2:10086846/150‑1 (MQ=255)
                                                                        agaTAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATcc                                                                               >  2:1160213/1‑150 (MQ=255)
                                                                           tAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtc                                                                            >  1:4068554/1‑150 (MQ=255)
                                                                            aaGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTAACGAATCAAATAACGATAAGGCAGTCCATCCGtct                                                                           >  2:2140229/1‑150 (MQ=255)
                                                                             aGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtctc                                                                          >  2:1693676/1‑150 (MQ=255)
                                                                             aGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGGTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGtctc                                                                          >  2:1476454/1‑150 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                        <  2:8504775/150‑1 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                        >  2:9097983/1‑150 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                        >  2:3976768/1‑150 (MQ=255)
                                                                                cAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCttt                                                                       >  2:8183015/1‑150 (MQ=255)
                                                                                 aacccGCTCGCACCTTGGCGCGCGCTCGGCCCCCGCGCCCCTGGGGGGTGGGGGTTGGGGGGCGGCCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                                                      <  1:3529122/147‑1 (MQ=255)
                                                                                  aaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGTTTAGGGTGTGGTGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAg                                                                     <  2:655430/150‑1 (MQ=255)
                                                                                  aaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAg                                                                     >  1:2228692/1‑150 (MQ=255)
                                                                                    ccGATCGCAACTTTGAGCGCGATCGGTCCCCGCGCCCCTCTGGGGAGAGGGTTAGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCa                                                                   <  2:3440813/150‑1 (MQ=255)
                                                                                    ccGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCa                                                                   >  1:7880896/1‑150 (MQ=255)
                                                                                     cGATCGCAACCGTGTGCGGGTTGGGTCCCCTCGCCCCTCTGGGTTGTGGGTTAGGGTGCGGGGAAAAGTCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGCATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAgtat                                                                  <  2:7697331/150‑4 (MQ=255)
                                                                                          gCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGt                                                             >  1:8696240/1‑150 (MQ=255)
                                                                                           cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                                            >  1:1616875/1‑150 (MQ=255)
                                                                                           cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                                            >  1:5850410/1‑150 (MQ=255)
                                                                                           cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                                            >  2:8749807/1‑150 (MQ=255)
                                                                                               tttGCGCGCGATGGGTCCCCTCGCCCGTGGGGGGTGTGGTTTTGGGTGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtg                                                        <  2:3203007/149‑1 (MQ=255)
                                                                                                ttGAGCGCGATGGGTCCCCTCGCCCCTGGGGGGTGTGGTTTCGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtgt                                                       <  2:4307423/150‑1 (MQ=255)
                                                                                                   ttgcgcTATCGGTCCCCAGCCCCCTGTGGGGATTGGTTTTGGGTTCGGGCAAAAGGCGGCATCGAAGCCAATCAGCACCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTc                                                     <  1:8940426/148‑1 (MQ=255)
                                                                                                     gcgcGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCGTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCa                                                   >  2:1053774/1‑150 (MQ=255)
                                                                                                      cgcgATCGGTCCCCTCGCCCCTGGGGGGTGCGGGTTCGGGTGCGGGGAAACGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAt                                                  <  1:9328110/150‑1 (MQ=255)
                                                                                                      cgcgATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCGTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAt                                                  >  2:8375912/1‑150 (MQ=255)
                                                                                                      cgcgATCGCTCCCCCCGCCCCTCTGGTTTGTGGTTTAGGGTGAGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAt                                                  <  2:1904761/150‑1 (MQ=255)
                                                                                                       gcgATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATa                                                 >  1:723746/1‑150 (MQ=255)
                                                                                                        cgATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaa                                                >  1:828249/1‑150 (MQ=255)
                                                                                                            gggTCCCCTCGCCCCTCGGGGGTGTGGTTTTGGGTGTGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGt                                            <  2:2710068/149‑1 (MQ=255)
                                                                                                             ggcccccTCGCCCTTGTGGGTTGTGGGTTATGGTCAGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCATTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTa                                           <  1:6101673/147‑1 (MQ=255)
                                                                                                               tCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACa                                         >  2:2846854/1‑150 (MQ=255)
                                                                                                                  ccgcGCCCGTGTGGGTTGTGGGTTAGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaaa                                      <  2:3634658/147‑1 (MQ=255)
                                                                                                                  ccTCGCCCCTGGGGGGTGTGGGTTTGGGTGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaaa                                      <  2:3016814/150‑1 (MQ=255)
                                                                                                                  ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaaa                                      >  1:8088481/1‑150 (MQ=255)
                                                                                                                    tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAcc                                    >  2:929629/1‑150 (MQ=255)
                                                                                                                      gCCCCTTTGGGTTGTGGTTTTGGTTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCATATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa                                  <  2:7765974/150‑1 (MQ=255)
                                                                                                                      gCCCCTCTGGGGAGAGGGTTAGGGTGAGGAGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa                                  <  1:516393/150‑1 (MQ=255)
                                                                                                                       ccgctcgGGGGTGTGGTTTTGGGGGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATATCGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAg                                 <  2:9601000/147‑1 (MQ=255)
                                                                                                                       ccccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAg                                 <  2:5822403/150‑1 (MQ=255)
                                                                                                                         ccTGTGGGGAGTGGGTTTGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGa                               <  1:1780528/150‑1 (MQ=255)
                                                                                                                          ctctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGaa                              >  2:5029190/1‑150 (MQ=255)
                                                                                                                          ctctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACAGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCAGTCTCTTTAGAAACCAGTTCGTGTTCCATAAAGGTACAAAACCAAGGaa                              >  2:8887896/1‑150 (MQ=255)
                                                                                                                           tctGGGGTGTGGTTTTGGGTGCGGGGACCAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat                             <  1:9557055/150‑1 (MQ=255)
                                                                                                                           tctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat                             >  2:5574845/1‑150 (MQ=255)
                                                                                                                           ggtGGGGTGTGGTTTTGGGGGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat                             <  1:2638239/148‑1 (MQ=255)
                                                                                                                            ctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAata                            >  2:2471655/1‑150 (MQ=255)
                                                                                                                             tGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAatat                           >  2:7518577/1‑150 (MQ=255)
                                                                                                                              gggtAGTGGGTTTGGGTGAGGTTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATc                          <  2:7378487/150‑1 (MQ=255)
                                                                                                                                ggAGCGGGTTCGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCATCCCCTATCACCCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGTTACAAAACCCAGGAATATcgc                        <  2:7875718/150‑1 (MQ=255)
                                                                                                                                 gtgtgggTTTGGGTGGGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATcgcg                       <  2:53823/146‑1 (MQ=255)
                                                                                                                                  agagGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCgg                      >  1:10280891/1‑150 (MQ=255)
                                                                                                                                    agGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGt                    >  2:6128097/1‑150 (MQ=255)
                                                                                                                                      ggTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAg                  >  2:7589867/1‑150 (MQ=255)
                                                                                                                                      ggTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAg                  >  2:1305861/1‑150 (MQ=255)
                                                                                                                                       gTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGt                 >  1:7031549/1‑150 (MQ=255)
                                                                                                                                          tgggTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGc              <  2:9679458/149‑1 (MQ=255)
                                                                                                                                            ggTGAGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCg            <  1:2751024/150‑1 (MQ=255)
                                                                                                                                            ggTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCg            <  2:6773486/150‑1 (MQ=255)
                                                                                                                                             gTGGGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCgg           <  2:9804026/150‑1 (MQ=255)
                                                                                                                                             gTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCgg           >  1:3149498/1‑150 (MQ=255)
                                                                                                                                                aGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtc        <  1:1278365/150‑1 (MQ=255)
                                                                                                                                                   ggAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcgtc     >  1:3029596/1‑150 (MQ=255)
                                                                                                                                                    gAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCTCGGGTAGTGGCCGGAtcgtcg    >  2:7046833/1‑150 (MQ=255)
                                                                                                                                                     aaaaGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCgg   >  2:5274096/1‑150 (MQ=255)
                                                                                                                                                     aaaaGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCgg   <  2:4068554/150‑1 (MQ=255)
                                                                                                                                                     aaaaGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCgg   <  1:5325461/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCGGC  >  NC_000913/3608572‑3608870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: