Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,106 G→C 5.0% intergenic (+89/‑109) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1060GC 5.0% 1588.2 / 12.0 456intergenic (+89/‑109)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (255/169);  new base C (25/5);  total (281/175)
Fisher's exact test for biased strand distribution p-value = 1.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 460 total aligned reads displayed.

GGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAA  >  NC_000913/3780957‑3781255
                                                                                                                                                     |                                                                                                                                                     
 gggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGc                                                                                                                                                      <  2:8195296/150‑1 (MQ=255)
  ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACGCCCTCACTCTGAAACGACACCGCa                                                                                                                                                     <  2:4674619/150‑1 (MQ=255)
  ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAACCCACACCCACAGGGAGAGGGGTCCCTTCGGCGCGGGCTTTACTCCCACACACAGAAACGACACCCCa                                                                                                                                                     >  2:1648912/1‑150 (MQ=255)
     tGGGTAAAGTGTTGCCTGGGGCACGGGCTCCCATGGCGAATGGGATTGCGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCActc                                                                                                                                                  <  1:4268808/150‑1 (MQ=255)
     tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCGCACCCAAACCCAAAACCACAGGGGGGGGGGGCCGTTCCGCGCGGTATGTACTCCCTCACACAGAAACGACACCCCAcac                                                                                                                                                  >  2:10131267/1‑148 (MQ=255)
      gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCActct                                                                                                                                                 <  2:2634175/150‑1 (MQ=255)
          aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                                                                                             <  2:2507097/150‑1 (MQ=255)
            agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                                                                                           <  2:9809017/150‑1 (MQ=255)
            agagTGGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                                                                                           <  1:3569532/150‑1 (MQ=255)
              agTTGCCTGCGTCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCTTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                                                                                                         <  1:6213417/150‑1 (MQ=255)
              agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCGAACCCGCACCCACAGGGGGCGGGGGCCGGGCGGCGCAGTTTGTTCTACCGCACTCTGGAAAGACACCCCCCGCttttttttt                                                                                                                                         >  1:8938130/1‑148 (MQ=255)
              agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCCAACCCAAAACCACACGGAGGGGGGGCCCTTCGGCGCGGGTTGTACACCCGCACTCGGTAAACACACCCCCCACtttttttat                                                                                                                                         >  1:8599441/1‑148 (MQ=255)
               gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                                                                        <  1:6012019/150‑1 (MQ=255)
                ttGCCTGCGTCACTGGCTCTCATGGCGAAAGGGATTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                                                       <  2:8837614/150‑1 (MQ=255)
                gggCCTGCGGCCCGGGCGCCCCTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                                                       <  1:2726113/148‑1 (MQ=255)
                   ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                                                                                    <  1:2048566/150‑1 (MQ=255)
                             tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                                          <  1:9305457/150‑1 (MQ=255)
                             tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                                          <  2:2031711/150‑1 (MQ=255)
                             tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                                          <  2:8541016/150‑1 (MQ=255)
                             tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCTAACCCCCGCCCTCCGGGCGAGGGGTCCGTTCGGGGCGGTATGTAATCCCTCACCCGGGAACGACACCGCCCGCGTTTTTTCTCCCGCGCCCCTCCgg                                                                                                                          >  2:774454/1‑150 (MQ=255)
                              ggCTTCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                         <  1:6492928/150‑1 (MQ=255)
                              ggCTCCCATGGCGAAAGGGACTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACGCCCTCACTCTGAAACGACACCGCACTCATTTTTTCTCCCTCGCCCCTCCggg                                                                                                                         <  1:9524025/150‑1 (MQ=255)
                               gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgggg                                                                                                                        <  2:5666943/150‑1 (MQ=255)
                                 ccccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgag                                                                                                                      <  1:7271234/149‑1 (MQ=255)
                                  cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                     <  1:1386723/150‑1 (MQ=255)
                                  cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                     <  1:6895880/150‑1 (MQ=255)
                                  cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                     <  1:7495743/150‑1 (MQ=255)
                                  cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                     <  2:3698104/150‑1 (MQ=255)
                                  cccAGGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGATCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                     <  2:8594446/150‑1 (MQ=255)
                                   ccATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCAAAACCACTCCCGCCCGGCGAGGGGGCCCTTAGGCGCGGTTAGTAATCCCCCACACGGAAAAGACACCCCCCACTTTTTTTATACCACGCCCCAACCGGGagag                                                                                                                    >  2:3165692/1‑150 (MQ=255)
                                    cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                                                                   <  2:3322213/150‑1 (MQ=255)
                                        gCGAAAGGGAAGGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGCGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                                                                               <  1:1245202/150‑1 (MQ=255)
                                          gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCGCCCTCAGGGTGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTTAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                             <  1:3758111/150‑1 (MQ=255)
                                               ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCTGGGAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                        <  1:4827893/150‑1 (MQ=255)
                                               ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                        <  1:56298/150‑1 (MQ=255)
                                                gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                       <  1:1124621/150‑1 (MQ=255)
                                                gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACGCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                       <  2:4556725/150‑1 (MQ=255)
                                                   tGCGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGa                                                                                                    <  2:1912297/150‑1 (MQ=255)
                                                   tGCGGCATAGGCGTTTGCGCCCCCGCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGa                                                                                                    <  2:7022862/150‑1 (MQ=255)
                                                                     gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                                                                  >  2:3095062/1‑150 (MQ=255)
                                                                     gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAc                                                                                  >  2:5540091/1‑150 (MQ=255)
                                                                          cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                                                             >  2:7412367/1‑150 (MQ=255)
                                                                           tCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCCGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAttt                                                                            >  2:3952193/1‑150 (MQ=255)
                                                                                 tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                                      >  1:8797150/1‑150 (MQ=255)
                                                                                    cccacgcccTCGGGGTGCGGGGACCGTGGGGGGTTGTTTGTACGCCCCCCCTCTGAACCCACCCGGCACTCTTTTTTGCGCCCGCGCCCCTCGGGGGAGCGGGCGGGGGTTTGGGTAAAAAGCCGCACTGCTCACCAATTTTCTGCTATc                                                                   <  2:5772935/144‑1 (MQ=255)
                                                                                     ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                  >  1:786781/1‑150 (MQ=255)
                                                                                      ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                 >  1:9124483/1‑150 (MQ=255)
                                                                                      ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                 >  2:8999950/1‑150 (MQ=255)
                                                                                       tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCACGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGCACAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                                >  2:934046/1‑150 (MQ=255)
                                                                                            cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                           >  2:604107/1‑150 (MQ=255)
                                                                                             tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                          >  1:2589721/1‑150 (MQ=255)
                                                                                             tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                                          >  1:5123341/1‑150 (MQ=255)
                                                                                                 ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGcccg                                                      >  1:1551265/1‑147 (MQ=255)
                                                                                                 ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                      >  2:10245477/1‑150 (MQ=255)
                                                                                                 ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                      >  1:8984452/1‑150 (MQ=255)
                                                                                                 ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                                      >  1:1980888/1‑150 (MQ=255)
                                                                                                   agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa                                                    >  2:5043101/1‑150 (MQ=255)
                                                                                                       gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                >  2:4054692/1‑150 (MQ=255)
                                                                                                       gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTATTTGTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTCTCCTGCCCCCGCACGACgggg                                                >  2:9617935/1‑150 (MQ=255)
                                                                                                              ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                         >  1:7632019/1‑150 (MQ=255)
                                                                                                                 ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                                      >  2:4864761/1‑150 (MQ=255)
                                                                                                                   cgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                                    >  2:6522409/1‑150 (MQ=255)
                                                                                                                   cgcTGTATGTACTCCCTCACTCAGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                                    >  2:474625/1‑150 (MQ=255)
                                                                                                                      tgtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTATGGGGGCAGTATGCTAAACa                                 >  1:3031968/1‑150 (MQ=255)
                                                                                                                      tgtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa                                 >  1:4122833/1‑150 (MQ=255)
                                                                                                                         atgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                              >  1:10042693/1‑150 (MQ=255)
                                                                                                                           gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                            >  2:7007297/1‑150 (MQ=255)
                                                                                                                           gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                            >  2:7623535/1‑150 (MQ=255)
                                                                                                                            taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                           >  2:1441561/1‑150 (MQ=255)
                                                                                                                            taCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                           >  2:4414794/1‑150 (MQ=255)
                                                                                                                               tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                        >  1:9915676/1‑150 (MQ=255)
                                                                                                                                 ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                      >  1:10193959/1‑150 (MQ=255)
                                                                                                                                 ccTCACTCTGAAACGACACCGCACTCTATTTTTCTCCCTCGCCCCTACGGGGAGAGGGACGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGATAACCTGCCCCCGCAAGAAGGGGGCAGTATGCTAAAAATCGTACTTTAc                      >  1:5422487/1‑150 (MQ=255)
                                                                                                                                  cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                     >  1:4631344/1‑150 (MQ=255)
                                                                                                                                  cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                     >  1:8061392/1‑150 (MQ=255)
                                                                                                                                  cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                     >  1:964982/1‑150 (MQ=255)
                                                                                                                                   tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                    >  2:6797424/1‑150 (MQ=255)
                                                                                                                                       tctcAAACCACACCTATCTTTTTTTTGCGCCCCCGCCCGGGGGGGGTGGGGGGGGGGGTGTGGGCAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                <  2:5113563/150‑1 (MQ=255)
                                                                                                                                       tctGAACCCACACCGCACTTTTTTTTCCTCCATCCCCCCCCGGGGGTGTGGGCGGGGGTGTGGGCACAAGGCCACACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                <  1:8460277/150‑1 (MQ=255)
                                                                                                                                       tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                >  2:2314344/1‑150 (MQ=255)
                                                                                                                                       tctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                >  2:8637656/1‑150 (MQ=255)
                                                                                                                                       tctGAAACGACACCGCACTCTTTTTTTCTCCATCGCCCATCAGGGGAGAGGGCCGGGGTGAGGGGAAAAGTCCGCACTGCTCACCAATTTTCTTCTATGCTGACCCCGCACTAATGGGGCAGTATGCTAAACTTCGTAGTTTACGAACCa                >  2:5876943/1‑150 (MQ=255)
                                                                                                                                        ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg               >  2:3386707/1‑150 (MQ=255)
                                                                                                                                         tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa              >  1:4588551/1‑150 (MQ=255)
                                                                                                                                         tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa              >  2:3610512/1‑150 (MQ=255)
                                                                                                                                          gAAACGACACCTCACTCATTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTCAACATCGTACTTTACGAACCAGaa             >  2:8781121/1‑150 (MQ=255)
                                                                                                                                           aaaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa            >  1:3835767/1‑150 (MQ=255)
                                                                                                                                           aaaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa            >  1:2347085/1‑150 (MQ=255)
                                                                                                                                           aaaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa            >  1:5807090/1‑150 (MQ=255)
                                                                                                                                            aaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAt           >  1:173467/1‑150 (MQ=255)
                                                                                                                                               gACACCGCACTCTTTTTTTCTCCGTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAATGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAGTAAGGGGGCAGTATGCTAATCATCGTACTTTACGATCCAGAATTTcc        >  2:5369974/1‑150 (MQ=255)
                                                                                                                                               gACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc        >  2:8302490/1‑150 (MQ=255)
                                                                                                                                               gACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc        >  2:8106911/1‑150 (MQ=255)
                                                                                                                                               gACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc        >  1:6655037/1‑150 (MQ=255)
                                                                                                                                               gACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc        >  2:9532052/1‑150 (MQ=255)
                                                                                                                                                cccccGAACTTTTTTGATCCCCCGCCCCCGTCGTGGGTGCGGGCGTGGGCGCGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCg       <  2:1529848/147‑1 (MQ=255)
                                                                                                                                                  cccGCACTTTTTTTTCCCCCCCGCCCCGGGGGTGTGTGGGGGGGGGTGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCa    <  2:2546842/149‑1 (MQ=255)
                                                                                                                                                    cGCCCTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGCGGGCCGGGGTGAGGGGACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCCCGAAGGGGGCCGTATGCTCAACAGCGTCCTTTCCGAACCAGAAATTCCGCCa  >  1:3170707/1‑149 (MQ=255)
                                                                                                                                                    cGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaa   >  2:6310419/1‑150 (MQ=255)
                                                                                                                                                     gCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaaa  >  1:3439989/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAA  >  NC_000913/3780957‑3781255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: