Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,828,980 A→C 6.1% Q12H (CAA→CAC xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,828,9800AC 6.1% 1526.0 / 17.1 595Q12H (CAA→CACxanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (298/244);  new base C (12/41);  total (310/285)
Fisher's exact test for biased strand distribution p-value = 5.58e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 614 total aligned reads displayed.

ATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGG  >  NC_000913/3828831‑3829129
                                                                                                                                                     |                                                                                                                                                     
atataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGGTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                       >  2:2554338/1‑150 (MQ=255)
 tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                      <  1:7571833/150‑1 (MQ=255)
   taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGTAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                    >  1:1683318/1‑150 (MQ=255)
   taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                    <  2:1579580/150‑1 (MQ=255)
   taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                    >  2:2486136/1‑150 (MQ=255)
    aaaaaaGTGCATTTATCTCATTTCAATATGTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCgg                                                                                                                                                   >  2:106277/1‑150 (MQ=255)
    aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                                   >  2:8925422/1‑150 (MQ=255)
     aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                  >  2:8252440/1‑150 (MQ=255)
      aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                 <  2:5627939/150‑1 (MQ=255)
               tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCCGACt                                                                                                                                        >  1:4982647/1‑150 (MQ=255)
                     tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                                                                                                                  >  2:2346900/1‑150 (MQ=255)
                       aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACac                                                                                                                                >  1:9948934/1‑149 (MQ=255)
                       aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                                >  2:1762154/1‑150 (MQ=255)
                        tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGc                                                                                                                               <  2:45507/150‑1 (MQ=255)
                         ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCg                                                                                                                              >  1:1573741/1‑150 (MQ=255)
                           aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGaa                                                                                                                            <  2:8030489/150‑1 (MQ=255)
                             atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACt                                                                                                                          >  2:9709140/1‑150 (MQ=255)
                              tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCACACTCAAAAAACCGAAcag                                                                                                                         >  1:19484/1‑148 (MQ=255)
                               atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGa                                                                                                                        >  2:490060/1‑150 (MQ=255)
                                tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACGGTTTCGCAGACTCAAAACAGCGAACTGAt                                                                                                                       >  2:2108198/1‑150 (MQ=255)
                                tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACGGTTGCGCAGACTCAAAAAAGCGAACTGAt                                                                                                                       >  1:6756335/1‑150 (MQ=255)
                                  tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAttt                                                                                                                     >  2:7459978/1‑150 (MQ=255)
                                    gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACACCGAACGGATATAc                                                                                                                   >  1:315842/1‑150 (MQ=255)
                                       gAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAGTTAACGt                                                                                                                >  1:3346544/1‑150 (MQ=255)
                                          gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCtt                                                                                                             >  2:7367865/1‑150 (MQ=255)
                                          gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCtt                                                                                                             <  2:544165/150‑1 (MQ=255)
                                          gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCtt                                                                                                             <  2:5768659/150‑1 (MQ=255)
                                          gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTCTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACACAAAAAAGCCAACAGATTTACCCTcgt                                                                                                             >  1:5212310/1‑148 (MQ=255)
                                             gtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                          <  2:7273626/150‑1 (MQ=255)
                                             gtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                          <  2:5518491/150‑1 (MQ=255)
                                               ggaaactgTTTGATTTTATCTTTCGGTCAGATAAAATGCCCGCTTTGATACCACGGGTTTTTTTTCTTTGTCTTTTACCACCCTCTAGTCATAAAATGCGAAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGa                                                                                                        <  1:4205156/143‑1 (MQ=255)
                                               ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATTCGCAACCCGTTGCGCAGAAACAAAAAAGCGAACTGATTTAACGTcattaata                                                                                                        >  1:7405388/1‑143 (MQ=255)
                                               ggCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGa                                                                                                        <  2:877097/150‑1 (MQ=255)
                                                gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGAt                                                                                                       >  2:3751549/1‑150 (MQ=255)
                                                 cAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                      <  2:3092697/150‑1 (MQ=255)
                                                  aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCCGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                     >  1:5583091/1‑150 (MQ=255)
                                                       tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAAAACCGAACAGATTTACCGTCTTGAAGATCGTccgc                                                                                                >  1:8046869/1‑150 (MQ=255)
                                                       tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgc                                                                                                >  1:953168/1‑150 (MQ=255)
                                                           cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                            >  2:7612612/1‑150 (MQ=255)
                                                            ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                           >  1:790929/1‑150 (MQ=255)
                                                            ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                           >  2:4440351/1‑150 (MQ=255)
                                                            ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                           >  1:7157138/1‑150 (MQ=255)
                                                             tttCTCTTTCGGTCAGATAAAATGCCCGCTTTGCTACCACGGGATTGTCTTCGTTGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                          <  1:5973970/150‑1 (MQ=255)
                                                               tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTCTATTCGATGTCTGTTTCCACCCACGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                        <  2:6879913/150‑1 (MQ=255)
                                                                ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCAGGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAATTGCGCTACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTATTGAAGATCGTCCGCCGCTTCCTc                                                                                       <  2:4983441/150‑1 (MQ=255)
                                                                ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTc                                                                                       <  2:419638/150‑1 (MQ=255)
                                                                 tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCTTCCTCa                                                                                      >  1:889564/1‑150 (MQ=255)
                                                                 tctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCCAACAGATTTACCGTCTTGAAGATCGTCCGCCCCTTCCTCa                                                                                      >  2:8138128/1‑150 (MQ=255)
                                                                  ctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaa                                                                                     <  2:8481609/150‑1 (MQ=255)
                                                                   tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaaa                                                                                    <  2:5145322/150‑1 (MQ=255)
                                                                   tttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaaa                                                                                    <  2:2932911/150‑1 (MQ=255)
                                                                   ttgcGGTCAGATAAAAGCCCCGCTGTGCAACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTAATAAACTGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaaa                                                                                    <  1:277740/147‑1 (MQ=255)
                                                                     tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                                  >  2:1991534/1‑150 (MQ=255)
                                                                       ggTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCCGCCTCAAAACACCGCACGGCTTTACCGTCTTGGAGAGCGTCCGCCGCGTCCGCAAAcccg                                                                                >  1:2201074/1‑149 (MQ=255)
                                                                         tCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                              >  2:3671253/1‑150 (MQ=255)
                                                                            gCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTg                                                                           >  2:7924730/1‑150 (MQ=255)
                                                                            gCTAAAAGGCCCGCTTTGCTACCAGGGGTTTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTg                                                                           <  1:10165804/150‑1 (MQ=255)
                                                                             cTAACATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                          <  2:1411212/150‑1 (MQ=255)
                                                                             cTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                          >  2:6792696/1‑150 (MQ=255)
                                                                              taaccggCCCGCTTTGCTCCCAGGGTTTTGTTTGCGTGGTCTGTTTCCACCCTCGCGTCAGAAAAGGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTGACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgcc                                                                         <  1:9080551/144‑1 (MQ=255)
                                                                              tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgcc                                                                         <  2:6597054/150‑1 (MQ=255)
                                                                              tAAAAGGCCCGCTTTGCTACCAGGGGTTTGTTTTCGTTTTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACGGTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgcc                                                                         <  1:5315597/150‑1 (MQ=255)
                                                                                aaaTGCCCGGTTTGCTACCACGGGTTTGTTTTCGATGTCTTTTTCCACCCGCGAGTAATAAATTGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                       <  1:1266352/150‑1 (MQ=255)
                                                                                  aTGCCCGATTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                     <  2:3022649/150‑1 (MQ=255)
                                                                                     cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGACCTGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCGGTTTGCCGCCTGTc                                                                  >  1:6399074/1‑150 (MQ=255)
                                                                                       cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                                <  2:6137638/150‑1 (MQ=255)
                                                                                            tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                           <  2:1254706/150‑1 (MQ=255)
                                                                                              cTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                         >  2:476372/1‑150 (MQ=255)
                                                                                                 cctcGGGTTTGTTTTCGATGTCTGTTACCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                      <  1:7805887/147‑1 (MQ=255)
                                                                                                  cACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGc                                                     >  2:7741973/1‑150 (MQ=255)
                                                                                                   aCGGGTTTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCg                                                    <  1:3452686/150‑1 (MQ=255)
                                                                                                   aCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGCGTCAGAAAATGCGCCACCCGGGGCGCAGACGCAAAACCGCGCACGGGGTACCCGGCGAGAACAGCGGCCGCCGCGTCCGCCAACCCGGTGTGCCGCCCGGCCGCCGCTGCTGGCg                                                    >  1:1229722/1‑150 (MQ=255)
                                                                                                   aCGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAAAAGCGAAATGAATTACCCTCGTGTAGATAGTCCGCCGCGTCCACAAACCCGGTTTGCCGCCGGTCAGCATCATCTGGCg                                                    >  1:3887385/1‑150 (MQ=255)
                                                                                                    cGGGATTGTTTCCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                                   <  2:2752266/150‑1 (MQ=255)
                                                                                                     gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAt                                                  >  2:8585420/1‑150 (MQ=255)
                                                                                                            ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGACACAAAAAAGCGAACTGATTTTCCCGGTTTAATATAGTCCCCCGCTTCCAAAAACACAGTTTTCCCCCGGTCAGCAACAGAAGGCGATTTTAGtt                                           >  1:2596136/1‑150 (MQ=255)
                                                                                                              ttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                         <  1:1762154/150‑1 (MQ=255)
                                                                                                               tCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACGGAGTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGGTTGCCGCCTGTCAGCATCTGCTGGCGATGGTCGTTGCg                                        >  2:427217/1‑150 (MQ=255)
                                                                                                               tCGATGTCTGTTTCCACCCTCGAGCAAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                        <  1:731002/150‑1 (MQ=255)
                                                                                                                  aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTgtggtg                                     <  2:7676099/150‑1 (MQ=255)
                                                                                                                    gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                   <  2:10138299/150‑1 (MQ=255)
                                                                                                                     tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATc                                  >  2:3917778/1‑150 (MQ=255)
                                                                                                                      cTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCCCACACAAAAAAGCGAACTGAGTTACCGGCGTGTAGATCGTCCGCCGCGGCCACAAACACAGTTTTGCGCCGGTCAGCATCTGCTGGGGCTGTTAGTTGCGGTGATCa                                 >  1:9876915/1‑150 (MQ=255)
                                                                                                                       tGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAACCCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                                <  2:9016316/150‑1 (MQ=255)
                                                                                                                       tGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                                <  2:3224518/150‑1 (MQ=255)
                                                                                                                         ttcccccccTCGTGTCCGAAAATGCGCACCCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                              <  1:113207/144‑1 (MQ=255)
                                                                                                                           tCCACCCTCGCGCCACAAAATGCGCAACGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                            <  1:4534776/150‑1 (MQ=255)
                                                                                                                             cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgc                          >  2:6377961/1‑150 (MQ=255)
                                                                                                                              aCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcg                         <  2:5078404/150‑1 (MQ=255)
                                                                                                                                  tCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAt                     <  1:8701506/150‑1 (MQ=255)
                                                                                                                                      gTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAAt                 <  1:8231544/150‑1 (MQ=255)
                                                                                                                                       tCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc                >  2:9615524/1‑150 (MQ=255)
                                                                                                                                           aaaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc            <  2:5275561/150‑1 (MQ=255)
                                                                                                                                            aaaTGCGCACACGGTTGCGCAGACTCAAATCAGCGAACTGAGTTACCGTCTTGAAGATCGTCCGCCGCGGCCTCAAACCCGGTTTGCCGCCTGGCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa           >  2:9376781/1‑150 (MQ=255)
                                                                                                                                            aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa           >  2:704443/1‑150 (MQ=255)
                                                                                                                                            aaaTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa           <  1:2459061/150‑1 (MQ=255)
                                                                                                                                                gcgcAACGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcg       <  1:6969423/150‑1 (MQ=255)
                                                                                                                                                 cgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc      >  2:2641943/1‑150 (MQ=255)
                                                                                                                                                  gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCt     >  2:9234112/1‑150 (MQ=255)
                                                                                                                                                  gcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCt     <  1:10004519/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGG  >  NC_000913/3828831‑3829129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: